##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527585_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2781506 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.98947656413468 31.0 31.0 33.0 28.0 33.0 2 29.72533476469222 31.0 30.0 31.0 25.0 33.0 3 31.549374691264372 31.0 31.0 33.0 30.0 34.0 4 35.42562482338704 35.0 35.0 37.0 33.0 37.0 5 35.74553209664117 37.0 35.0 37.0 35.0 37.0 6 33.82793170318525 35.0 33.0 35.0 32.0 37.0 7 35.37118165483015 35.0 35.0 37.0 35.0 37.0 8 36.29296359598002 37.0 35.0 37.0 35.0 37.0 9 38.518097030889024 39.0 39.0 39.0 37.0 39.0 10 36.440942065197774 39.0 35.0 39.0 32.0 39.0 11 36.83204638062978 38.0 37.0 39.0 33.0 39.0 12 36.90468149268777 39.0 37.0 39.0 33.0 39.0 13 36.90147459685509 39.0 37.0 39.0 33.0 39.0 14 37.84231887330101 40.0 38.0 40.0 33.0 40.0 15 37.9182532771815 40.0 38.0 40.0 33.0 40.0 16 37.872105614728135 40.0 38.0 40.0 33.0 40.0 17 37.900170267473804 40.0 38.0 40.0 34.0 40.0 18 37.72728802310691 40.0 37.0 40.0 33.0 40.0 19 37.61434776700104 40.0 37.0 40.0 33.0 40.0 20 37.52449248716343 39.0 37.0 40.0 33.0 40.0 21 37.52667224158424 39.0 37.0 40.0 33.0 40.0 22 37.40417277546768 39.0 37.0 40.0 33.0 40.0 23 37.13293733682401 39.0 36.0 40.0 32.0 40.0 24 36.82458855023142 38.0 36.0 40.0 32.0 40.0 25 36.209732785045226 37.0 35.0 39.0 31.0 40.0 26 35.223095330371386 37.0 34.0 39.0 30.0 40.0 27 34.87381260367585 36.0 34.0 38.0 29.0 40.0 28 34.80949025455994 36.0 34.0 38.0 29.0 40.0 29 34.389733834836235 35.0 33.0 38.0 28.0 40.0 30 33.9221630296681 35.0 33.0 38.0 26.0 39.0 31 33.776529333389895 35.0 33.0 38.0 25.0 39.0 32 33.26037297780411 35.0 33.0 38.0 24.0 39.0 33 32.87924599120045 35.0 32.0 38.0 23.0 39.0 34 32.28936734272728 35.0 31.0 38.0 21.0 39.0 35 32.042832192344726 35.0 31.0 37.0 20.0 39.0 36 31.369110115167825 34.0 30.0 37.0 17.0 39.0 37 30.37514569445473 34.0 28.0 37.0 12.0 38.0 38 29.583666905625947 33.0 26.0 36.0 10.0 38.0 39 28.48069894510384 33.0 23.0 36.0 9.0 38.0 40 27.793145871337327 32.0 22.0 35.0 9.0 38.0 41 27.357967230701643 32.0 21.0 35.0 8.0 38.0 42 26.900365125942564 31.0 20.0 35.0 8.0 37.0 43 26.429974625256964 31.0 18.0 35.0 8.0 37.0 44 26.064586953973855 30.0 18.0 34.0 8.0 37.0 45 25.765205252118815 30.0 18.0 34.0 8.0 36.0 46 25.414969085092753 29.0 18.0 34.0 8.0 36.0 47 25.210173912980952 29.0 18.0 33.0 8.0 35.0 48 24.601187270493035 28.0 16.0 33.0 8.0 35.0 49 24.071016204890444 27.0 15.0 33.0 8.0 35.0 50 25.541089611167475 30.0 15.0 34.0 8.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 11.0 13 21.0 14 54.0 15 130.0 16 320.0 17 737.0 18 1509.0 19 3205.0 20 5863.0 21 9714.0 22 15492.0 23 23785.0 24 35833.0 25 52229.0 26 74937.0 27 108676.0 28 145743.0 29 169132.0 30 159509.0 31 141506.0 32 156840.0 33 207952.0 34 302591.0 35 532258.0 36 527313.0 37 104637.0 38 1502.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.48732808773376 0.22649600612042542 0.16911701790325098 0.11705888824255638 8 98.56771835113783 0.7308271130819061 0.33471076460018423 0.36674377118007295 9 96.48244512145578 1.9009845745434308 0.6935811031865471 0.9229892008142351 10 50.92985598449186 33.35955414081436 7.7799580515015965 7.930631823192185 11 27.160717970768356 43.8922655568602 15.27643657788263 13.670579894488814 12 29.93389192761044 36.73376940405665 16.904475489177447 16.427863179155462 13 20.079446170527763 37.542468001147576 19.217467084378033 23.160618743946625 14 20.836949479886076 44.244916243215 18.65029232365488 16.267841953244034 15 24.6240705574606 38.87142432912242 17.732731836638138 18.771773276778838 16 21.58485367279452 39.909782686070066 22.221990533185977 16.283373107949433 17 28.589943721135242 38.87976513442718 15.168113964161861 17.36217718027572 18 25.434854355877718 39.658767588493426 16.54449064643398 18.361887409194875 19 22.461105602504542 39.61019677829204 20.066359734618587 17.862337884584825 20 19.68861472885552 44.12196126846391 16.852129745540726 19.33729425713984 21 21.133587344409825 38.71097168224695 22.318269311660664 17.837171661682554 22 20.498284023115534 39.6461844770423 15.544277093056783 24.31125440678539 23 19.73919883688908 47.01823400704511 14.77444233447636 18.468124821589456 24 20.396145109879324 45.761972111510815 15.488156415984722 18.35372636262514 25 20.639214871368246 41.427305927076915 15.601799888261969 22.33167931329287 26 26.757626983368006 39.999805860566184 15.014132631746973 18.22843452431884 27 22.23709026692734 39.5331881362111 16.0523471817066 22.177374415154954 28 25.627951189032128 43.48094161939611 14.108975497446346 16.78213169412541 29 29.4433662914982 38.46783001726403 14.84487180685571 17.243931884382057 30 24.70578887839897 42.55388987117051 15.450478985125324 17.289842265305197 31 29.21104610236325 39.227526383189534 14.664969264851488 16.89645824959572 32 25.527933428869108 39.68720542037299 17.995611010725845 16.789250140032056 33 28.88877464222619 39.60444449877153 15.091285080815933 16.415495778186347 34 23.151522951954803 41.58218605316688 15.844869649750889 19.421421345127424 35 23.15436313996806 45.98278774160473 15.53172993335265 15.33111918507456 36 27.559854265998347 40.903417069745664 14.778037509176684 16.7586911550793 37 24.037409949861694 40.28285396472271 18.22178345112324 17.457952634292358 38 29.138135959440675 38.51241018354805 14.800255688824688 17.549198168186585 39 27.299168148477836 35.39489039390891 15.2007221986938 22.105219258919448 40 27.174307731135578 34.43656062579049 19.304901733089917 19.084229909984014 41 26.65379833802264 33.27477992138072 16.36893826581715 23.70248347477949 42 27.153455717873697 35.28955177518941 16.980836999812333 20.576155507124557 43 26.41597753159619 35.131112426146125 18.27211589692778 20.1807941453299 44 26.256711292371833 35.72402863772359 18.174614759054986 19.84464531084959 45 29.114228047683522 33.37303604594058 17.429730512894814 20.083005393481084 46 25.996528499309367 33.65292039636082 20.10813566463635 20.242415439693463 47 28.206086918381622 33.3765233653999 17.633900484126226 20.783489232092258 48 27.291654233354162 33.71655498855656 18.152971807359037 20.838818970730244 49 26.649088659165216 33.576810547954956 17.816175841432663 21.957924951447165 50 23.891805374498563 33.953153435584895 20.00696744856923 22.148073741347314 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 122.0 4 244.0 5 274.5 6 305.0 7 1179.0 8 2053.0 9 26480.0 10 50907.0 11 40629.0 12 30351.0 13 22974.5 14 15598.0 15 15156.0 16 14714.0 17 17721.0 18 20728.0 19 26296.5 20 31865.0 21 40838.5 22 49812.0 23 60520.0 24 71228.0 25 82756.5 26 94285.0 27 97231.0 28 100177.0 29 97644.5 30 95112.0 31 93343.0 32 91574.0 33 89282.0 34 86990.0 35 83410.0 36 79830.0 37 78500.0 38 77170.0 39 76857.5 40 76545.0 41 72860.0 42 69175.0 43 72633.0 44 76091.0 45 82180.5 46 88270.0 47 92055.5 48 95841.0 49 107290.0 50 118739.0 51 145580.5 52 172422.0 53 176958.0 54 181494.0 55 181556.5 56 181619.0 57 176120.5 58 170622.0 59 161368.0 60 152114.0 61 145331.5 62 138549.0 63 122312.5 64 106076.0 65 91423.0 66 76770.0 67 65366.5 68 53963.0 69 47208.0 70 40453.0 71 33208.0 72 25963.0 73 27858.0 74 29753.0 75 19722.5 76 9692.0 77 6202.0 78 2712.0 79 2076.5 80 1441.0 81 817.0 82 193.0 83 121.5 84 50.0 85 30.5 86 11.0 87 8.0 88 5.0 89 2.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2781506.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.590564435844545 #Duplication Level Percentage of deduplicated Percentage of total 1 81.85205581823841 22.583444202594514 2 7.761914636984536 4.283112118744936 3 2.70356946414141 2.2377902252152584 4 1.3236940387810145 1.4608586268132355 5 0.823667828665367 1.1362730150261986 6 0.5649608687733376 0.9352553552172894 7 0.42880062254894663 0.8281595844596876 8 0.33875373002125025 0.7477125292827194 9 0.2779934924931155 0.6902997630639093 >10 2.8970782174854155 19.01646820188643 >50 0.6695212932193941 12.641215837029279 >100 0.32140822243053463 16.884429710513402 >500 0.022341007225535524 4.254680863349856 >1k 0.01241167068085307 6.918398305044489 >5k 0.0013064916506161125 2.158593043750673 >10k+ 5.22596660246445E-4 3.223308618008202 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36749 1.321190750622145 No Hit CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGA 26623 0.9571433604673153 No Hit CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14785 0.5315465794429349 No Hit CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11263 0.4049245264975161 No Hit CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 8924 0.32083339025693275 No Hit CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA 7552 0.27150759336848457 No Hit CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 6058 0.2177956833456408 No Hit CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 5659 0.20345093629134722 No Hit CAAAGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 5515 0.19827388472288035 No Hit CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA 5398 0.194067530323501 Illumina Single End Adapter 2 (97% over 34bp) CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 5361 0.19273731568438104 No Hit CAAAGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG 5165 0.1856907732717456 No Hit CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 5149 0.1851155453196937 No Hit CAAAGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA 5102 0.18342581321054136 No Hit CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 4812 0.17299980657960112 No Hit CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA 4770 0.17148983320546496 No Hit CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAA 4437 0.15951790145338532 No Hit CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAA 4425 0.15908648048934643 No Hit CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA 4390 0.15782816934423294 Illumina Single End Adapter 2 (97% over 34bp) CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAA 4127 0.14837285988238028 No Hit CAAAGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA 4093 0.14715050048427003 No Hit CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3840 0.13805470849244977 No Hit CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAA 3497 0.12572325927033773 No Hit CAAAGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA 3168 0.11389513450627106 No Hit CAAAGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 3060 0.11001234582992091 No Hit CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAA 3023 0.10868213119080096 No Hit CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2991 0.10753167528669721 No Hit CAAAGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC 2982 0.10720810956366804 No Hit CAAAGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 2972 0.10684859209363562 No Hit CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2927 0.10523076347848971 No Hit CAAAGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC 2907 0.10451172853842487 No Hit CAAAGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT 2832 0.1018153475131817 No Hit CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 2795 0.10048513287406176 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.003343512471301518 0.0 0.0 0.0 0.0 8 0.019306088140741022 0.0 0.0 0.0 0.0 9 0.02955233603666503 0.0 0.0 0.0 0.0 10 0.18579862851275533 0.0 0.0 0.0 0.0 11 0.39899248824198114 0.0 0.0 0.0 0.0 12 0.5860134761528467 0.0 0.0 0.0 0.0 13 0.6949113178256671 0.0 0.0 0.0 0.0 14 0.793347201120544 0.0 0.0 0.0 0.0 15 0.8898776418242491 0.0 0.0 0.0 0.0 16 0.9970857513879172 0.0 0.0 0.0 0.0 17 1.0921421704644894 0.0 0.0 0.0 0.0 18 1.1938496627366613 0.0 0.0 0.0 0.0 19 1.3035743945905562 0.0 0.0 0.0 0.0 20 1.4311311929580595 0.0 0.0 0.0 0.0 21 1.564691933075104 0.0 0.0 0.0 0.0 22 1.7172711473568636 0.0 0.0 0.0 0.0 23 1.8973174963491002 0.0 0.0 0.0 0.0 24 2.0981080033622073 0.0 0.0 0.0 0.0 25 2.3016667948945644 0.0 0.0 0.0 0.0 26 2.512020466610534 0.0 0.0 0.0 0.0 27 2.726005264773831 0.0 0.0 0.0 0.0 28 3.005422242483029 0.0 0.0 0.0 0.0 29 3.164544674719379 0.0 0.0 0.0 0.0 30 3.331612443043445 0.0 0.0 0.0 0.0 31 3.45963661412199 0.0 0.0 0.0 0.0 32 3.578888558931744 0.0 0.0 0.0 0.0 33 3.6852338265673343 0.0 0.0 0.0 0.0 34 3.8012860658938 0.0 0.0 0.0 0.0 35 3.9828064365131697 0.0 0.0 0.0 0.0 36 4.07829427655378 0.0 0.0 0.0 0.0 37 4.166843429422766 0.0 0.0 0.0 0.0 38 4.253702850182599 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAACG 20 7.858412E-4 44.0 9 TTATCGA 20 7.858412E-4 44.0 28 GTTATCG 20 7.858412E-4 44.0 27 TGGGATT 3360 0.0 41.57738 6 GTGGGAT 11520 0.0 40.77257 5 TGGGGTC 5530 0.0 40.499096 6 TAATTGG 60 3.6379788E-12 40.333332 14 TGGGGCT 9930 0.0 40.300102 6 GTGGGAC 8635 0.0 40.229298 5 TAGTTCG 55 7.8216544E-11 40.0 37 TGGGGTT 4395 0.0 39.895336 6 TGGGGAC 5990 0.0 39.813023 6 TGGGGGC 10155 0.0 39.688824 6 GGAAGCA 16180 0.0 39.64895 8 GCGTAAC 50 1.3496901E-9 39.6 36 TGGGGGT 8880 0.0 39.565315 6 GTGGGGC 27390 0.0 39.421688 5 TAAGGTA 190 0.0 39.368423 33 TGGGACT 2805 0.0 39.294117 6 AGTGGGG 155775 0.0 39.172783 4 >>END_MODULE