SRR1527584_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2346568
Sequences flagged as poor quality	0
Sequence length	52
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30737	1.3098704150060856	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	18322	0.7807998745401795	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12228	0.5211014554021022	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9349	0.3984116377620423	No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7723	0.3291189515922829	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA	6376	0.27171596987600616	No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6260	0.2667725802107589	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5444	0.2319983908414331	No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	5200	0.22160022637315435	No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	4906	0.2090712904974414	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4850	0.20668482652111508	No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4595	0.1958178923432008	No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4590	0.19560481520245737	No Hit
CAAAGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	4565	0.19453942949874029	No Hit
CAAAGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4491	0.1913858878157377	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	4060	0.17301863828365513	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4021	0.17135663658585645	No Hit
CAAAGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3666	0.1562281595930738	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAA	3639	0.15507754303305935	No Hit
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA	3542	0.15094384650263704	No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAA	3437	0.14646922654702527	No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3241	0.1381166026298833	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAA	3223	0.137349524923207	No Hit
CAAAGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2922	0.12452228105045327	No Hit
CAAAGTGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2828	0.12051643080447702	Illumina Single End Adapter 2 (100% over 34bp)
CAAAGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	2820	0.12017550737928753	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAA	2770	0.11804473597185336	No Hit
CAAAGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2714	0.1156582719955271	No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2561	0.1091381114887785	No Hit
CAAAGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	2501	0.10658118579985751	No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2456	0.10466349153316673	No Hit
CAAAGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2452	0.104493029820572	No Hit
CAAAGTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2401	0.10231964298498915	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAC	20	6.312805E-4	46.0	26
CGACGTA	55	1.8189894E-12	46.0	28
CGACCAA	20	6.312805E-4	46.0	45
TTGTACG	20	6.312805E-4	46.0	45
CGAATAC	25	3.418253E-5	46.0	38
ATACGCT	40	5.6152203E-9	46.0	15
CAATACG	40	5.6152203E-9	46.0	13
ATATAGG	45	3.110472E-10	45.999996	22
TGGGATT	2785	0.0	43.1921	6
GTGGGAC	6990	0.0	42.545063	5
GTGGGAT	9465	0.0	42.379295	5
TGGGGCT	8740	0.0	41.63158	6
TGGGACT	2280	0.0	41.258774	6
TGGGGTC	4520	0.0	41.165928	6
GTGGGGC	23295	0.0	40.994205	5
TGGGGGT	7945	0.0	40.991817	6
GTGGGGT	23635	0.0	40.978634	5
TGGGGTT	3465	0.0	40.88889	6
TTATTCG	45	1.5896148E-8	40.888885	26
AGTGGGG	133590	0.0	40.88315	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content