FastQCFastQC Report
Fri 17 Jun 2016
SRR1527583_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527583_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2988820
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA383461.2829812434338637No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG293960.9835319624467181No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA158630.5307445747820211No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111000.37138402446450436No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT99690.3335430035933914No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA75940.25408020556607624No Hit
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG69730.23330277500819724No Hit
CAAAGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA62340.20857729806411895No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA61740.2065698168507973No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC60350.20191915203993546No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC60070.20098232747371875No Hit
CAAAGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG59560.19927596844239534No Hit
CAAAGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA59390.19870718209862087No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT52520.17572152220608803No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAA48340.16173606975328056No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA46040.15404072510221425Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA45570.15246819815177895No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAA45480.1521670759697807No Hit
CAAAGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG44430.14865398384646783No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAA41400.13851620371919351No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39880.13343058464544535No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAA38080.12740814100548042No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA37920.126872812681928Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGGAG37280.12473149938771824No Hit
CAAAGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC36580.1223894379721763No Hit
CAAAGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC35090.11740419295909423No Hit
CAAAGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG35030.11720344483776206No Hit
CAAAGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG34440.11522942164466243No Hit
CAAAGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT32760.10960847424736184No Hit
CAAAGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC32700.10940772612602967No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32390.10837052749914683No Hit
CAAAGTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG31850.10656379440715734No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31760.10626267222515909No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAA30580.10231462583895985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATA253.4184784E-546.024
TCGGTTA253.4184784E-546.036
TACGTAC206.313085E-446.017
TGGGATT39150.043.473826
GTGGGAT125550.042.061335
GTGGGAC99100.041.7527775
TGGGGCT111550.041.731966
TGGGGGT99650.041.614656
TGGGGGC116900.041.415746
GTGGGGC297700.041.3567355
TGGGGTC59350.041.2721146
TGGGGTT49100.041.0346226
GTGGGGT291000.040.9890025
AGTGGGG1680750.040.976474
GGAAGCA178300.040.9562578
GTGGGGG537650.040.879385
AAGCAGT170150.040.82280310
TGGGGAC70500.040.8127676
TGGGACT32550.040.559146
GAAGCAG174650.040.4952779
Produced by FastQC (version 0.11.5)