##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2988820
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.534069632831685	31.0	30.0	33.0	28.0	33.0
2	29.30080499996654	31.0	30.0	31.0	25.0	33.0
3	31.16829250339599	31.0	31.0	33.0	30.0	34.0
4	35.17699961857857	35.0	35.0	37.0	33.0	37.0
5	35.545429969017874	37.0	35.0	37.0	33.0	37.0
6	33.32846909482672	35.0	33.0	35.0	28.0	36.0
7	35.31771836376898	35.0	35.0	37.0	33.0	37.0
8	36.23535977409145	37.0	35.0	37.0	35.0	37.0
9	38.48294042464919	39.0	39.0	39.0	37.0	39.0
10	36.14526535555838	39.0	35.0	39.0	31.0	39.0
11	36.482786852336375	38.0	35.0	39.0	32.0	39.0
12	36.68927235497621	39.0	35.0	39.0	32.0	39.0
13	36.669659932682464	39.0	35.0	39.0	33.0	39.0
14	37.52759818256034	40.0	37.0	40.0	33.0	40.0
15	37.73503924625772	40.0	37.0	40.0	33.0	40.0
16	37.60383629659866	40.0	37.0	40.0	33.0	40.0
17	37.798299328832115	40.0	37.0	40.0	33.0	40.0
18	37.757838210397416	40.0	37.0	40.0	33.0	40.0
19	37.48243119358141	40.0	37.0	40.0	32.0	40.0
20	37.576607490581566	40.0	37.0	40.0	33.0	40.0
21	37.6632011964588	40.0	37.0	40.0	33.0	40.0
22	37.67499280652565	40.0	37.0	40.0	33.0	40.0
23	37.506261668484555	40.0	37.0	40.0	33.0	40.0
24	37.34854725276196	39.0	37.0	40.0	33.0	40.0
25	36.86359901231924	38.0	36.0	40.0	32.0	40.0
26	36.32183403483649	38.0	35.0	40.0	31.0	40.0
27	35.96257252025883	38.0	35.0	40.0	31.0	40.0
28	35.748523832147804	38.0	35.0	40.0	30.0	40.0
29	35.691703080145345	38.0	35.0	40.0	30.0	40.0
30	35.304924016836075	37.0	35.0	40.0	29.0	40.0
31	35.0844182654024	37.0	34.0	40.0	28.0	40.0
32	34.62648403048695	37.0	34.0	40.0	25.0	40.0
33	34.447829243647995	36.0	34.0	40.0	25.0	40.0
34	33.959381294290054	36.0	33.0	40.0	22.0	40.0
35	33.64139325887808	36.0	33.0	40.0	21.0	40.0
36	33.14124069030587	36.0	33.0	40.0	17.0	40.0
37	32.33411915070162	35.0	31.0	40.0	12.0	40.0
38	31.78254193962835	35.0	30.0	40.0	10.0	40.0
39	31.09025468244993	35.0	28.0	39.0	9.0	40.0
40	30.641436419724172	35.0	25.0	40.0	9.0	40.0
41	30.377639670505417	35.0	24.0	40.0	8.0	40.0
42	30.13511017726059	35.0	23.0	40.0	8.0	40.0
43	29.996702377526915	35.0	23.0	39.0	8.0	40.0
44	29.768020489691583	35.0	23.0	39.0	8.0	40.0
45	29.52753628522293	35.0	23.0	39.0	8.0	40.0
46	29.19738525571965	35.0	20.0	39.0	8.0	40.0
47	29.154851078351992	35.0	22.0	38.0	8.0	40.0
48	29.031804859442857	35.0	21.0	38.0	8.0	40.0
49	28.86838284005059	35.0	20.0	38.0	8.0	40.0
50	28.467332258215617	35.0	18.0	37.0	8.0	40.0
51	28.28535442080821	34.0	18.0	37.0	8.0	40.0
52	27.745814067090024	34.0	15.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	1.0
13	24.0
14	29.0
15	130.0
16	363.0
17	993.0
18	2425.0
19	4725.0
20	8221.0
21	13566.0
22	21791.0
23	32977.0
24	48000.0
25	68462.0
26	99102.0
27	137292.0
28	158323.0
29	140051.0
30	99391.0
31	88565.0
32	101746.0
33	129683.0
34	187091.0
35	225928.0
36	370582.0
37	482421.0
38	528877.0
39	38056.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.50823401877665	0.22279695665848062	0.16340897076438193	0.1055600538004965
8	98.61751460442582	0.7037559973501248	0.32186615453590384	0.3568632436881445
9	96.53997229675926	1.8745859569997525	0.6585876700503878	0.9268540761906037
10	51.52364478289091	33.09095228217156	7.403691088790894	7.98171184614664
11	27.224824512683938	43.42660314103894	15.36452513031899	13.984047215958137
12	29.615266225466907	36.24898789488829	17.249884569830236	16.885861309814576
13	19.936597051679257	37.10711919754284	19.584183724680642	23.372100026097257
14	20.814669334386146	43.513025207272435	19.000575477947816	16.6717299803936
15	24.48166835072035	38.26503436138677	18.137124350077958	19.116172937814923
16	21.7268687977195	39.293466986971445	22.25741262438019	16.72225159092886
17	28.48953767707657	38.2969867706988	15.404172884282092	17.809302667942532
18	25.396410623590583	39.11587181563292	16.728909736953046	18.75880782382345
19	22.61169960051124	39.08515735306911	20.068153987192268	18.234989059227388
20	19.863491277494127	43.48415093582083	17.0704492073795	19.581908579305544
21	21.300513246030206	38.20678394818022	22.334499902971743	18.158202902817834
22	20.837956116460678	39.092718865639284	15.738920376603478	24.330404641296568
23	19.94556380109876	46.27063523397194	14.946400251604311	18.837400713324993
24	20.568853259814908	45.115028673523334	15.602344738057159	18.7137733286046
25	20.790077689522956	40.8762655496149	15.799546309245791	22.534110451616357
26	26.630108203237395	39.65297341425713	15.157854939407525	18.559063443097944
27	22.32918007775644	39.130426054429506	16.119471898608815	22.42092196920524
28	25.694153545546406	42.877021700871914	14.27964882461975	17.14917592896193
29	29.35623423290797	38.03173158637857	15.008498337136395	17.603535843577063
30	24.82026351536727	41.854711892987865	15.687395025461553	17.63762956618331
31	29.16826707530062	38.82415133731707	14.758868048259849	17.248713539122463
32	25.85829859275567	39.2549568056959	17.889702290536064	16.997042311012372
33	28.8606540373793	39.14411038470031	15.206369068729463	16.78886650919092
34	23.33733714308657	41.18474849606199	15.980286534485181	19.49762782636626
35	23.45875629847231	45.38965210350573	15.551789669501675	15.599801928520286
36	27.78665158825222	40.57741851299175	14.667996065336823	16.967933833419206
37	24.741938290027502	39.9270949739362	17.690627070215	17.640339665821294
38	29.530550518264732	38.17258985151331	14.65360911664135	17.64325051358061
39	27.951867292108588	35.28887654659699	14.902469871052789	21.856786290241633
40	27.754732636960405	33.93115008598711	18.83800295768899	19.476114319363493
41	27.15543257874345	32.793878520620176	16.358730201216535	23.69195869941984
42	27.812882676106288	34.05691878400171	17.132045422608254	20.998153117283742
43	27.102736196893755	34.445567146900785	18.27922056196091	20.17247609424455
44	27.296960004282628	34.47106215830997	18.141406976666378	20.09057086074103
45	29.780415013282834	32.16075240395875	17.70805869875068	20.350773884007737
46	27.103371899277978	32.47488975582337	19.802597680690038	20.61914066420862
47	28.976284955266628	31.982086575973128	17.837574695030145	21.2040537737301
48	28.730535796735836	32.09443860787869	18.153853360188972	21.0211722351965
49	27.99195000033458	31.472320179870316	18.274369149028715	22.26136067076639
50	24.180579626742325	33.25533153552238	19.79389859543231	22.77019024230298
51	23.425030614088506	33.87420453556922	19.554640292824594	23.146124557517684
52	26.99991970075147	30.305237518485555	20.221927048132706	22.47291573263027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	141.0
4	282.0
5	321.5
6	361.0
7	1252.0
8	2143.0
9	27291.5
10	52440.0
11	41223.0
12	30006.0
13	22322.0
14	13830.5
15	13023.0
16	14623.0
17	16223.0
18	18598.0
19	20973.0
20	25542.0
21	30111.0
22	36992.5
23	43874.0
24	54459.5
25	65045.0
26	75154.0
27	85263.0
28	92944.0
29	100625.0
30	108169.0
31	115713.0
32	115851.0
33	115989.0
34	108671.5
35	101354.0
36	95552.0
37	89750.0
38	87771.0
39	80880.0
40	75968.0
41	77113.5
42	78259.0
43	82289.5
44	86320.0
45	87307.0
46	88294.0
47	90161.5
48	92029.0
49	98323.5
50	104618.0
51	138588.5
52	172559.0
53	172325.5
54	172092.0
55	182703.0
56	193314.0
57	194179.5
58	195045.0
59	179269.5
60	163494.0
61	160633.0
62	157772.0
63	137328.0
64	106989.5
65	97095.0
66	81424.0
67	65753.0
68	57412.5
69	49072.0
70	41992.5
71	34913.0
72	35956.0
73	36999.0
74	26980.0
75	16961.0
76	10933.0
77	4905.0
78	3318.0
79	1731.0
80	1291.5
81	852.0
82	510.5
83	169.0
84	123.0
85	77.0
86	50.5
87	24.0
88	20.0
89	8.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2988820.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.483884814060694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.0854880964882	22.56028099749832
2	7.835270393448629	4.306873379611242
3	2.769724045433712	2.2836832989430307
4	1.2979646631997066	1.4269244518440727
5	0.795373461579976	1.0929976301112394
6	0.5316985459529178	0.8767884955664129
7	0.39417937904575445	0.758350645484004
8	0.29808709682356577	0.6554073146925312
9	0.2650648537919286	0.6556510718885884
>10	2.62788972560838	17.19822475950921
>50	0.7137002105189669	13.488724725321251
>100	0.3490106042840556	18.083707281439633
>500	0.02310283144744022	4.415628773316891
>1k	0.011734771528858524	6.793318968491158
>5k	0.0012223720342560962	2.2217733302831513
>10k+	4.889488137024386E-4	3.1816648759993624
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	38346	1.2829812434338637	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGAAG	29396	0.9835319624467181	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15863	0.5307445747820211	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11100	0.37138402446450436	No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9969	0.3335430035933914	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA	7594	0.25408020556607624	No Hit
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6973	0.23330277500819724	No Hit
CAAAGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	6234	0.20857729806411895	No Hit
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6174	0.2065698168507973	No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6035	0.20191915203993546	No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6007	0.20098232747371875	No Hit
CAAAGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5956	0.19927596844239534	No Hit
CAAAGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	5939	0.19870718209862087	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5252	0.17572152220608803	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	4834	0.16173606975328056	No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	4604	0.15404072510221425	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA	4557	0.15246819815177895	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAA	4548	0.1521670759697807	No Hit
CAAAGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4443	0.14865398384646783	No Hit
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAA	4140	0.13851620371919351	No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3988	0.13343058464544535	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAAAA	3808	0.12740814100548042	No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3792	0.126872812681928	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGGAG	3728	0.12473149938771824	No Hit
CAAAGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3658	0.1223894379721763	No Hit
CAAAGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	3509	0.11740419295909423	No Hit
CAAAGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	3503	0.11720344483776206	No Hit
CAAAGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3444	0.11522942164466243	No Hit
CAAAGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	3276	0.10960847424736184	No Hit
CAAAGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3270	0.10940772612602967	No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3239	0.10837052749914683	No Hit
CAAAGTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	3185	0.10656379440715734	No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3176	0.10626267222515909	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAAAA	3058	0.10231462583895985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0034461760828688247	0.0	0.0	0.0	0.0
8	0.018368453101893056	0.0	0.0	0.0	0.0
9	0.028004362925836954	0.0	0.0	0.0	0.0
10	0.1678588874539116	0.0	0.0	0.0	0.0
11	0.3728896353744956	0.0	0.0	0.0	0.0
12	0.5393098279588601	0.0	0.0	0.0	0.0
13	0.6471115691142324	0.0	0.0	0.0	0.0
14	0.740492903553911	0.0	0.0	0.0	0.0
15	0.834610314438474	0.0	0.0	0.0	0.0
16	0.9402038262591926	0.0	0.0	0.0	0.0
17	1.0321130078091019	0.0	0.0	0.0	0.0
18	1.1230519067725724	0.0	0.0	0.0	0.0
19	1.2262698991575272	0.0	0.0	0.0	0.0
20	1.3391238013664255	0.0	0.0	0.0	0.0
21	1.4573979028512924	0.0	0.0	0.0	0.0
22	1.5859101585240998	0.0	0.0	0.0	0.0
23	1.7391478911409854	0.0	0.0	0.0	0.0
24	1.89583179984074	0.0	0.0	0.0	0.0
25	2.0482330819520747	0.0	0.0	0.0	0.0
26	2.1943777142818903	0.0	0.0	0.0	0.0
27	2.33811336915572	0.0	0.0	0.0	0.0
28	2.553817225527131	0.0	0.0	0.0	0.0
29	2.6533882937078848	0.0	0.0	0.0	0.0
30	2.7705248225052026	0.0	0.0	0.0	0.0
31	2.8409539550725706	0.0	0.0	0.0	0.0
32	2.9058290562830815	0.0	0.0	0.0	0.0
33	2.9668564851680594	0.0	0.0	0.0	0.0
34	3.036616457330987	0.0	0.0	0.0	0.0
35	3.189285403604098	0.0	0.0	0.0	0.0
36	3.2471008625477613	0.0	0.0	0.0	0.0
37	3.2995295802356783	0.0	0.0	0.0	0.0
38	3.3514898856404867	0.0	0.0	0.0	0.0
39	3.4048219698743987	0.0	0.0	0.0	3.345802022202742E-5
40	3.4599942452205217	0.0	0.0	0.0	3.345802022202742E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATA	25	3.4184784E-5	46.0	24
TCGGTTA	25	3.4184784E-5	46.0	36
TACGTAC	20	6.313085E-4	46.0	17
TGGGATT	3915	0.0	43.47382	6
GTGGGAT	12555	0.0	42.06133	5
GTGGGAC	9910	0.0	41.752777	5
TGGGGCT	11155	0.0	41.73196	6
TGGGGGT	9965	0.0	41.61465	6
TGGGGGC	11690	0.0	41.41574	6
GTGGGGC	29770	0.0	41.356735	5
TGGGGTC	5935	0.0	41.272114	6
TGGGGTT	4910	0.0	41.034622	6
GTGGGGT	29100	0.0	40.989002	5
AGTGGGG	168075	0.0	40.97647	4
GGAAGCA	17830	0.0	40.956257	8
GTGGGGG	53765	0.0	40.87938	5
AAGCAGT	17015	0.0	40.822803	10
TGGGGAC	7050	0.0	40.812767	6
TGGGACT	3255	0.0	40.55914	6
GAAGCAG	17465	0.0	40.495277	9
>>END_MODULE