FastQCFastQC Report
Fri 17 Jun 2016
SRR1527582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248476
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC22370.9002881566026497No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG21650.871311514995412No Hit
CGAGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC21440.8628599945266343No Hit
CGAGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC17440.7018786522642027No Hit
CGAGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT13990.5630322445628552No Hit
CGAGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC10740.4322349039746294No Hit
CGAGTAGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTG8740.35174423284341344No Hit
CGAGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG8490.3416828989520114No Hit
CGAGTAGGGATCTCTGTTTGTCTCTGTATCTGTCTCTGTCTCTCTGTGTC8230.3312191117049534No Hit
CGAGTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT7620.30666945700993253No Hit
CGAGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC6690.26924129493391713No Hit
CGAGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA6620.2664241214443246No Hit
CGAGTAGGGGATGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTG5790.23302049292486998No Hit
CGAGTAGGGGCTCCTGCTTTTGCTACCCGGAGGACGAAGGTGCACAGACT5700.2293984127239653No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTGCTCGAGTAGGGGCCGGTAG5690.22899595936830922No Hit
CGAGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA5520.22215425232215585No Hit
CGAGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG5460.2197395321882194No Hit
CGAGTAGGGGGCGCCTGCTCTCCTGCTTTTGCTACCCGGAGGACGAAGGT5340.21491009192034644No Hit
CGAGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT5250.2112880117194417No Hit
CGAGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT5250.2112880117194417No Hit
CGAGTAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT5170.20806838487419307No Hit
CGAGTAGGGGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAGCC4940.19881195769410326No Hit
CGAGTAGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT4900.19720214427147892No Hit
CGAGTAGGGACCCTTCTCCTGCCCAAACACGGAAGTTGTGTCCCTGACTC4880.19639723756016678No Hit
CGAGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC4710.18955553051401342No Hit
CGAGTAGGGGTTTACGTTCCGAGAAGACCTAGCGGTTGGCTTCTCCGACT4430.1782868365556432No Hit
CGAGTAGGGGGACACTGGAAGAGGGGCCTGTCCATCCTCACCATCGCCTT4420.17788438319998712No Hit
CGAGTAGGGGGTCTCGGTCCATGCGAGGGGGGGAGGGGGTGGCGCGCGCG4400.17707947648867498No Hit
CGAGTAGGGATCTGGGACCCAGTGCTATCCGTGATGGCTGTGAAGGACCT4370.17587211642170672No Hit
CGAGTAGGGCAGCCGGCCCAACCTGCAGTCCTTCTTTGAGAGGGTCCAGA4320.17385984964342632No Hit
CGAGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4300.17305494293211415No Hit
CGAGTAGGGGACGGGCTTGAGCCGCCATTTTGACTGAGCAACCATAGTGA4240.1706402227981777No Hit
CGAGTAGGGAGCAGATGTGGATCAGCAAGCAGGAGTACGATGAGTCCGGC4200.1690304093755534No Hit
CGAGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCATCGACTG3980.16017643555111963No Hit
CGAGTAGGGGTGCTCGAGTAGGGGAGACGTCATTGCAGGGTTGTTTGTGC3910.15735926206152706No Hit
CGAGTAGGGGTCAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTG3780.15212736843799804No Hit
CGAGTAGGGGGGTGGGTCTGGGCCTGGAGAGTGAAAGCGAAAGCTGGGGC3780.15212736843799804No Hit
CGAGTAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGC3760.15132246172668587No Hit
CGAGTAGGGATGCAAGTCGATAGCAAATGCAGAGCGGCAGTTCTTTTCTA3750.1509200083710298No Hit
CGAGTAGGGCAAGCTGATGGTTGAACTGTCCAAATCCCAGGATGATGAAA3750.1509200083710298No Hit
CGAGTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG3710.1493101949484055No Hit
CGAGTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA3700.1489077415927494No Hit
CGAGTAGGGGTCTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCGTTCC3680.14810283488143725No Hit
CGAGTAGGGGAACAAAGATGGCGGCTGCCGCTGCTAGCCGAGCGGTCGGC3660.14729792817012508No Hit
CGAGTAGGGGGGGCAGCAGAGCCATGGAGCCCGGGGCTGCTGAGCTTTAT3500.1408586744796278No Hit
CGAGTAGGGACTCGCTTATGTCCTCCGCGCCACCACGGTCGCCCACCCCG3500.1408586744796278No Hit
CGAGTAGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCT3460.1392488610570035No Hit
CGAGTAGGGGGTCCCTCCGGACAGCTGGCTGGCTGGCTTTGAGGACCTTC3460.1392488610570035No Hit
CGAGTAGGGGAATGCTGTACATCTATTTGATTGTAAATTGTGTTTGTGTA3450.13884640770134743No Hit
CGAGTAGGGGACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAGATG3450.13884640770134743No Hit
CGAGTAGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTC3420.13763904763437917No Hit
CGAGTAGGGGTCTGTCTGCCTGCCAGTGTGCTACTACTTTCCCAGATGTG3380.13602923421175486No Hit
CGAGTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT3360.1352243275004427No Hit
CGAGTAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAA3340.13441942078913055No Hit
CGAGTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT3340.13441942078913055No Hit
CGAGTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA3320.13361451407781838No Hit
CGAGTAGGGGATGTGATAAAAGAATTGTATAAAGGGAATAATTGTCTGAT3230.12999243387691367No Hit
CGAGTAGGGGGAGCAGGTCTGACTCCTGACTGGCTGGAGTGCTTTTGGAG3190.12838262045428936No Hit
CGAGTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATC3170.1275777137429772No Hit
CGAGTAGGGGGGGAAGGTGCCCGGGCGGCGGCCATTTTGTCTTCTCGGCT3160.1271752603873211No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG3150.12677280703166505No Hit
CGAGTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT3090.12435808689772855No Hit
CGAGTAGGGGAGCTGCGCACTCCTGGACTGGCGACGTTGTGCTTCTAACA3070.12355318018641641No Hit
CGAGTAGGGCCATCAGGCCTCTGACCCAGACTGCTGTGTCCCTTCTTCCT3040.12234582011944817No Hit
CGAGTAGGGGGGCTGGAGCGATGGCTCAGTGGTTAAAAGCATTGGCTGCT3000.12073600669682384No Hit
CGAGTAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA2990.12033355334116774No Hit
CGAGTAGGGGATTGACAAGGACATTGGCTCCAAGTCTGACCCACTCTGCG2960.11912619327419953No Hit
CGAGTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT2960.11912619327419953No Hit
CGAGTAGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCC2940.11832128656288736No Hit
CGAGTAGGGGTGCTCGAGTAGGGCAAGATGAAAAGCTGAGACTGTCAACC2930.11791883320723129No Hit
CGAGTAGGGGCTTCCGCTTCCGGTCCGCAGAGACCCGGAGTCCGAATTCA2920.11751637985157522No Hit
CGAGTAGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGG2920.11751637985157522No Hit
CGAGTAGGGGGTCCGCGCTGGAGACCACAGCTCGGCACAGGCGTCCCGTC2880.11590656642895088No Hit
CGAGTAGGGGTGCTCGAGTAGGGGTGCTCGAGTAGGGGGGGTTGGGGATT2880.11590656642895088No Hit
CGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAA2870.1155041130732948No Hit
CGAGTAGGGGACTGGAGAGATGGCTCAGTGGTTAAAAGCACTCACTGGCT2860.11510165971763872No Hit
CGAGTAGGGCAAGCAGGAGTACGATGAGTCCGGCCCCTCCATCGTGCACC2850.11469920636198265No Hit
CGAGTAGGGACTTCTTTTCCTTTTTTTTAAAGGAAGTTTTCTTGTTACAT2840.11429675300632655No Hit
CGAGTAGGGGCCTTGCTTCGCTGCGCTTCTCCTGCAGCCTGGTAGGCACC2830.11389429965067048No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGC2820.11349184629501441No Hit
CGAGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG2790.11228448622804617No Hit
CGAGTAGGGGGTCCCCCTCGCCACCAGCGGCAAACTAGGACTGTGCGTTT2770.11147957951673403No Hit
CGAGTAGGGGGGGCTGGAAAGATGGCTCAGTGGTTAAAAGCATTGACTGC2760.11107712616107793No Hit
CGAGTAGGGGGAGTGCTGCAGCTTCCCTGGCCCGCTGCGCAGCGTCGGCC2740.11027221944976577No Hit
CGAGTAGGGGCAGGGATGAGTCACTCTGGGTGCCGTGCTCAGTTCTCATG2730.1098697660941097No Hit
CGAGTAGGGAGCCAAGCGGTAGAAGCCGGGTGGCATGGCAGCGAGCACGG2710.10906485938279753No Hit
CGAGTAGGGGCTCGGATGTGGCTGAACCGAAGCGCGCAGGCGGGAGACTC2700.10866240602714146No Hit
CGAGTAGGGACATTAAGTGCTCTAAGGCCTATATTCATACACGAATGCGA2680.10785749931582929No Hit
CGAGTAGGGGGGAGCTGGAGAGATGGCTCAGAGGTTGAGAGCACTGACTG2660.10705259260451715No Hit
CGAGTAGGGGCTGGTGATATGGCTCAGCGGGTAAGAGCACTGACTGCTCT2660.10705259260451715No Hit
CGAGTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC2650.10665013924886105No Hit
CGAGTAACAAAGTTTGGATTGCTACTGACCGCTCTCGTGCTCGTCGCTGC2650.10665013924886105No Hit
CGAGTAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGC2650.10665013924886105No Hit
CGAGTAGGGATGTAAAACATGAAATAATAATAATAAGCCGGGCGGTGGTG2640.10624768589320498No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC2630.1058452325375489No Hit
CGAGTAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTT2630.1058452325375489No Hit
CGAGTAGGGGAGGCCGGTGTGTGTCGGTGTGTATGTGTGTGTGTGTGTGT2620.10544277918189282No Hit
CGAGTAGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGC2600.10463787247058066No Hit
CGAGTAGGGGAAGCAAAGATGTCGGAAGATTCAGAGGTTGTGCTGCAGCT2590.10423541911492458No Hit
CGAGTAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTG2590.10423541911492458No Hit
CGAGTAGGGAGCTATAAACACAGTGGAGCACATGCCCCTGTGGTATGATA2590.10423541911492458No Hit
CGAGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT2580.10383296575926851No Hit
CGAGTAGGGGCTCTTCTCGCTGAGCGCCGCCAACATGGTGTTCAGGCGTT2570.10343051240361241No Hit
CGAGTAGGGGGGCTGGAAAGATGGCTCAGTGGTTAAAAGCATTGACTGCT2570.10343051240361241No Hit
CGAGTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC2560.10302805904795634No Hit
CGAGTAGGGTCCCCACTGTGGCCGCGGCGCGGGAAGGACAGTGAGCGGCC2550.10262560569230027No Hit
CGAGTAGGGGTTTTTCAGATCCAAGAGAACTCCTCTGAAAATTCTTTTTA2550.10262560569230027No Hit
CGAGTAGGGGGCTGTTGAGCTGGGAGGCGCGGTTGGAAGTCGTTACTTTG2540.10222315233664417No Hit
CGAGTAGGGGGGAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACT2540.10222315233664417No Hit
CGAGTAGGGGTGGACCAGCTGGTGAAAGCAGACAGTGAGTGGCGACGATG2530.1018206989809881No Hit
CGAGTAGGGAGTCTCAGCTCAGCGCACAGCTGGGCTTGAATTTGGGAGGA2520.10141824562533203No Hit
CGAGTAGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAG2500.10061333891401986No Hit
CGAGTAGGGGGTGACGGGCAGGAAACCATTACACCACCACCCCGGCTGTG2500.10061333891401986No Hit
CGAGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC2490.10021088555836379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGC408.267307E-944.00000412
TATTTGA408.267307E-944.00000424
ATTTACG207.844556E-444.00000411
GTTGAAC750.044.00000420
TAATATG207.844556E-444.00000435
GAGTGTC207.844556E-444.00000441
CTAGACG207.844556E-444.00000434
AAACTCG207.844556E-444.00000424
CAGAACC207.844556E-444.00000440
CCTTATA207.844556E-444.00000422
GATACTT207.844556E-444.00000441
ACTATGA207.844556E-444.00000429
GATACCG207.844556E-444.00000426
CCCCTGG207.844556E-444.00000417
GCCCACT207.844556E-444.00000435
GCAAGAA207.844556E-444.0000049
ACGCCTG207.844556E-444.00000412
ATCTATA207.844556E-444.00000432
CAAGTTC207.844556E-444.00000433
GTACATC408.267307E-944.00000417
Produced by FastQC (version 0.11.5)