FastQCFastQC Report
Fri 17 Jun 2016
SRR1527581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192955
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT17800.9224948822264258No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC17650.9147210489492368No Hit
CGAGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT16300.844756549454536No Hit
CGAGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA14770.7654634500272084No Hit
CGAGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG12170.6307170065559327No Hit
CGAGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC8540.44259024124795937No Hit
CGAGTAGGGATCTCTGTTTGTCTCTGTATCTGTCTCTGTCTCTCTGTGTCTG7220.3741805084086963No Hit
CGAGTAGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCT6660.3451581975071908No Hit
CGAGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT6630.343603430851753No Hit
CGAGTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG6210.32183669767562384No Hit
CGAGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC5140.2663833536316758No Hit
CGAGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC5080.2632738203208002No Hit
CGAGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG4660.24150708714467103No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTGCTCGAGTAGGGGCCGGTAGTG4550.23580627607473248No Hit
CGAGTAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG4470.23166023166023164No Hit
CGAGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA4430.22958720945298128No Hit
CGAGTAGGGGGCGCCTGCTCTCCTGCTTTTGCTACCCGGAGGACGAAGGTGC4370.22647767614210568No Hit
CGAGTAGGGGATGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT4330.22440465393485529No Hit
CGAGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA4030.2088569873804773No Hit
CGAGTAGGGGCTCCTGCTTTTGCTACCCGGAGGACGAAGGTGCACAGACTTT4000.2073022207250395No Hit
CGAGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC3970.2057474540696017No Hit
CGAGTAGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG3880.20108315410328834No Hit
CGAGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC3750.19434583192972454No Hit
CGAGTAGGGGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAGCCGA3720.19279106527428674No Hit
CGAGTAGGGGGACACTGGAAGAGGGGCCTGTCCATCCTCACCATCGCCTTCC3650.18916327641159855No Hit
CGAGTAGGGGAACAAAGATGGCGGCTGCCGCTGCTAGCCGAGCGGTCGGCGC3550.1839807208934726No Hit
CGAGTAGGGGGTCTCGGTCCATGCGAGGGGGGGAGGGGGTGGCGCGCGCGCC3510.18190769868622217No Hit
CGAGTAGGGACCCTTCTCCTGCCCAAACACGGAAGTTGTGTCCCTGACTCGA3500.18138944313440958No Hit
CGAGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCATCGACTGCT3420.17724339871990877No Hit
CGAGTAGGGATCTGGGACCCAGTGCTATCCGTGATGGCTGTGAAGGACCTGA3410.17672514316809618No Hit
CGAGTAGGGCAGCCGGCCCAACCTGCAGTCCTTCTTTGAGAGGGTCCAGAGA3390.175688632064471No Hit
CGAGTAGGGGTGCTCGAGTAGGGGAGACGTCATTGCAGGGTTGTTTGTGCAG3340.173097354305408No Hit
CGAGTAGGGGTTTACGTTCCGAGAAGACCTAGCGGTTGGCTTCTCCGACTCA3330.1725790987535954No Hit
CGAGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT3140.16273224326915603No Hit
CGAGTAGGGGGGTGGGTCTGGGCCTGGAGAGTGAAAGCGAAAGCTGGGGCTG3140.16273224326915603No Hit
CGAGTAGGGGACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAGATGTG3140.16273224326915603No Hit
CGAGTAGGGGACGGGCTTGAGCCGCCATTTTGACTGAGCAACCATAGTGACA3110.16117747661371823No Hit
CGAGTAGGGGAGGCCGGTGTGTGTCGGTGTGTATGTGTGTGTGTGTGTGTGT2970.15392189888834185No Hit
CGAGTAGGGAGCAGATGTGGATCAGCAAGCAGGAGTACGATGAGTCCGGCCC2940.15236713223290405No Hit
CGAGTAGGGATGCAAGTCGATAGCAAATGCAGAGCGGCAGTTCTTTTCTACT2890.14977585447384104No Hit
CGAGTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA2870.14873934337021585No Hit
CGAGTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC2830.14666632116296546No Hit
CGAGTAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT2820.14614806561115287No Hit
CGAGTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG2810.14562981005934025No Hit
CGAGTAGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTCT2780.14407504340390248No Hit
CGAGTAGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCC2760.14303853230027727No Hit
CGAGTAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC2730.14148376564483947No Hit
CGAGTAGGGGAATGCTGTACATCTATTTGATTGTAAATTGTGTTTGTGTATT2720.14096551009302688No Hit
CGAGTAGGGGCTCTTCTCGCTGAGCGCCGCCAACATGGTGTTCAGGCGTTAC2710.14044725454121426No Hit
CGAGTAGGGGGGGCAGCAGAGCCATGGAGCCCGGGGCTGCTGAGCTTTATGA2710.14044725454121426No Hit
CGAGTAGGGACTCGCTTATGTCCTCCGCGCCACCACGGTCGCCCACCCCGCG2680.13889248788577646No Hit
CGAGTAGGGGTCTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCGTTCCGC2680.13889248788577646No Hit
CGAGTAGGGGATGTGATAAAAGAATTGTATAAAGGGAATAATTGTCTGATAC2650.13733772123033866No Hit
CGAGTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA2650.13733772123033866No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT2600.1347464434712757No Hit
CGAGTAGGGGGGGAAGGTGCCCGGGCGGCGGCCATTTTGTCTTCTCGGCTCC2570.1331916768158379No Hit
CGAGTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA2530.1311186546085875No Hit
CGAGTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG2500.1295638879531497No Hit
CGAGTAGGGGTCAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT2500.1295638879531497No Hit
CGAGTAGGGGGTCCCTCCGGACAGCTGGCTGGCTGGCTTTGAGGACCTTCCA2490.1290456324013371No Hit
CGAGTAGGGGATTGACAAGGACATTGGCTCCAAGTCTGACCCACTCTGCGTC2490.1290456324013371No Hit
CGAGTAGGGACGCAGGAGTGCAGTGCTCGAGTAGGGGGGGTTGGGGATTTAG2480.1285273768495245No Hit
CGAGTAGGGGTGCTCGAGTAGGGGTGCTCGAGTAGGGGGGGTTGGGGATTTA2450.1269726101940867No Hit
CGAGTAGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAG2450.1269726101940867No Hit
CGAGTAGGGCAAGCTGATGGTTGAACTGTCCAAATCCCAGGATGATGAAATT2380.1233448213313985No Hit
CGAGTAGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC2340.12127179912414811No Hit
CGAGTAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAAC2310.11971703246871032No Hit
CGAGTAGGGGGAGTGCTGCAGCTTCCCTGGCCCGCTGCGCAGCGTCGGCCCT2310.11971703246871032No Hit
CGAGTAGGGGTCTGTCTGCCTGCCAGTGTGCTACTACTTTCCCAGATGTGTA2310.11971703246871032No Hit
CGAGTAGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC2290.11868052136508514No Hit
CGAGTAGGGACATTAAGTGCTCTAAGGCCTATATTCATACACGAATGCGAGC2270.11764401026145993No Hit
CGAGTAGGGGACTGGAGAGATGGCTCAGTGGTTAAAAGCACTCACTGGCTGC2270.11764401026145993No Hit
CGAGTAGGGGGTCACGGCTAGGGTCGAGGTTGCTACGGCGGACCAGCGGTGG2260.11712575470964734No Hit
CGAGTAGGGGCTTCCGCTTCCGGTCCGCAGAGACCCGGAGTCCGAATTCAGA2250.11660749915783472No Hit
CGAGTAGGGGAGCTGCGCACTCCTGGACTGGCGACGTTGTGCTTCTAACAGC2250.11660749915783472No Hit
CGAGTAGGGGGGCTGGAGCGATGGCTCAGTGGTTAAAAGCATTGGCTGCTCT2250.11660749915783472No Hit
CGAGTAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA2240.11608924360602213No Hit
CGAGTAGGGATGTAAAACATGAAATAATAATAATAAGCCGGGCGGTGGTGGT2220.11505273250239692No Hit
CGAGTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG2220.11505273250239692No Hit
CGAGTAGGGGTGCTCGAGTAGGGCAAGATGAAAAGCTGAGACTGTCAACCCA2200.11401622139877174No Hit
CGAGTAGGGGAAGCAAAGATGTCGGAAGATTCAGAGGTTGTGCTGCAGCTCC2190.11349796584695913No Hit
CGAGTAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA2170.11246145474333394No Hit
CGAGTAGGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTC2140.11090668808789615No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT2130.11038843253608353No Hit
CGAGTAGGGGCTGGTGATATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTC2130.11038843253608353No Hit
CGAGTAGGGGCCCCTTTTGCTTCCTTTTCCTGCCCATGCCACCTCTCGAAGC2110.10935192143245835No Hit
CGAGTAGGGGGTGACGGGCAGGAAACCATTACACCACCACCCCGGCTGTGCT2110.10935192143245835No Hit
CGAGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT2090.10831541032883314No Hit
CGAGTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT2080.10779715477702055No Hit
CGAGTAGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCCCTTC2070.10727889922520795No Hit
CGAGTAGGGAGTCTCAGCTCAGCGCACAGCTGGGCTTGAATTTGGGAGGAGC2070.10727889922520795No Hit
CGAGTAGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGC2070.10727889922520795No Hit
CGAGTAGGGGGTCCGCGCTGGAGACCACAGCTCGGCACAGGCGTCCCGTCCT2060.10676064367339536No Hit
CGAGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG2060.10676064367339536No Hit
CGAGTAGGGGATCTTTTCCTGCAGTGTATTAGGCTGGCCCTTTCTGTAGGCA2060.10676064367339536No Hit
CGAGTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA2050.10624238812158276No Hit
CGAGTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT2040.10572413256977015No Hit
CGAGTAGGGGGTTAAGAGCGCCGACTGCTCTTCCGAAGGTCCCGAGTTTCAA2040.10572413256977015No Hit
CGAGTAGGGGCAGGGATGAGTCACTCTGGGTGCCGTGCTCAGTTCTCATGTT2030.10520587701795756No Hit
CGAGTAGGGGGGCTGGAAAGATGGCTCAGTGGTTAAAAGCATTGACTGCTCT2000.10365111036251975No Hit
CGAGTAGGGGGGTTCGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGT2000.10365111036251975No Hit
CGAGTAGGGCAAGCAGGAGTACGATGAGTCCGGCCCCTCCATCGTGCACCGC2000.10365111036251975No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTC1990.10313285481070716No Hit
CGAGTAGGGGAGCCAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC1990.10313285481070716No Hit
CGAGTAGGGGGGGTTGGAGAGATGGCTCAATGGTTAAGAGCACCGACTGCTC1980.10261459925889455No Hit
CGAGTAGGGGAATTGTGTTCGCAGCCTTCGCCGCGCCGCTGCCGCTCTCCAA1980.10261459925889455No Hit
CGAGTAGGGGGGAGCTGGAGAGATGGCTCAGAGGTTGAGAGCACTGACTGCT1980.10261459925889455No Hit
CGAGTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTTTG1960.10157808815526936No Hit
CGAGTAGGGGGTCCCCCTCGCCACCAGCGGCAAACTAGGACTGTGCGTTTGC1960.10157808815526936No Hit
CGAGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC1950.10105983260345676No Hit
CGAGTAGGGGGGGCTGGAAAGATGGCTCAGTGGTTAAAAGCATTGACTGCTC1950.10105983260345676No Hit
CGAGTAGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG1950.10105983260345676No Hit
CGAGTAGGGGGAGCAGGTCTGACTCCTGACTGGCTGGAGTGCTTTTGGAGAA1940.10054157705164417No Hit
CGAGTAGGGATACATAGATAGCAATGTGCAGGGATTTTTACCCAGCCAGCTA1930.10002332149983156No Hit
CGAGTAGGGAGAGCTGCAGGTGCCAAAGTGCATTGCACAGAGGCCACATCTC1930.10002332149983156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG206.29834E-446.00000414
TCACGCT206.29834E-446.00000437
CATGGGA206.29834E-446.00000445
TTCTAAT206.29834E-446.00000430
CTATGGG206.29834E-446.00000416
CGTGGCC206.29834E-446.00000441
TCGTTGC206.29834E-446.00000446
AAATCGC206.29834E-446.00000425
TGCCCAC206.29834E-446.00000432
CATATTG206.29834E-446.00000421
TCACCTA206.29834E-446.00000441
GGTGTTA206.29834E-446.00000428
CATATGT206.29834E-446.00000443
GATCGCT206.29834E-446.00000419
TTCAGCA206.29834E-446.00000423
CAAAAGG206.29834E-446.00000445
CATGCTC206.29834E-446.00000430
CCGATTA206.29834E-446.00000420
CACGGGA206.29834E-446.00000433
CGTGCCC206.29834E-446.00000445
Produced by FastQC (version 0.11.5)