FastQCFastQC Report
Fri 17 Jun 2016
SRR1527580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265519
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT23290.8771500344608107No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23090.8696176168183821No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC22670.8537995397692821No Hit
CGAGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA20160.7592676983568031No Hit
CGAGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG15200.5724637408245737No Hit
CGAGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC10890.4101401406302374No Hit
CGAGTAGGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCGACTGCT9310.35063404125505143No Hit
CGAGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT9290.3498807994908086No Hit
CGAGTAGGGATCTCTGTTTGTCTCTGTATCTGTCTCTGTCTCTCTGTGTCTG8260.3110888486323013No Hit
CGAGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC8010.3016733265792655No Hit
CGAGTAGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG7770.2926344254083512No Hit
CGAGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC7270.2738033813022797No Hit
CGAGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA6360.23953088102922956No Hit
CGAGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG6310.2376477766186224No Hit
CGAGTAGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG6200.23350494691528667No Hit
CGAGTAGGGGCTCCTGCTTTTGCTACCCGGAGGACGAAGGTGCACAGACTTT6000.22597252927285807No Hit
CGAGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC5780.2176868698661866No Hit
CGAGTAGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG5700.21467390280921517No Hit
CGAGTAGGGGATGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCT5670.2135440401628509No Hit
CGAGTAGGGGGCGCCTGCTCTCCTGCTTTTGCTACCCGGAGGACGAAGGTGC5590.21053107310587943No Hit
CGAGTAGGGATCTGGGACCCAGTGCTATCCGTGATGGCTGTGAAGGACCTGA5270.19847920487799367No Hit
CGAGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5170.19471299605677936No Hit
CGAGTAGGGGGACACTGGAAGAGGGGCCTGTCCATCCTCACCATCGCCTTCC5100.19207664988192935No Hit
CGAGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA4980.1875571992964722No Hit
CGAGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT4950.1864273366501079No Hit
CGAGTAGGGGTTTACGTTCCGAGAAGACCTAGCGGTTGGCTTCTCCGACTCA4920.1852974740037436No Hit
CGAGTAGGGGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAGCCGA4750.17889491900767931No Hit
CGAGTAGGGGGTCTCGGTCCATGCGAGGGGGGGAGGGGGTGGCGCGCGCGCC4710.17738843547919358No Hit
CGAGTAGGGAGCAGATGTGGATCAGCAAGCAGGAGTACGATGAGTCCGGCCC4700.17701181459707213No Hit
CGAGTAGGGGAACAAAGATGGCGGCTGCCGCTGCTAGCCGAGCGGTCGGCGC4650.175128710186465No Hit
CGAGTAGGGACCCTTCTCCTGCCCAAACACGGAAGTTGTGTCCCTGACTCGA4620.1739988475401007No Hit
CGAGTAGGGGTGCTCGAGTAGGGGAGACGTCATTGCAGGGTTGTTTGTGCAG4620.1739988475401007No Hit
CGAGTAGGGGACGGGCTTGAGCCGCCATTTTGACTGAGCAACCATAGTGACA4520.1702326387188864No Hit
CGAGTAGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG4440.16721967166191495No Hit
CGAGTAGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA4340.16345346284070067No Hit
CGAGTAGGGGTGCAGTGCTCGAGTAGGGGTGCTCGAGTAGGGGCCGGTAGTG4260.1604404957837292No Hit
CGAGTAGGGCAGCCGGCCCAACCTGCAGTCCTTCTTTGAGAGGGTCCAGAGA4250.1600638749016078No Hit
CGAGTAGGGATGCAAGTCGATAGCAAATGCAGAGCGGCAGTTCTTTTCTACT4210.15855739137312208No Hit
CGAGTAGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA4170.15705090784463635No Hit
CGAGTAGGGGGCTGGCGAGATGGCTCAGTAGGTAAGAGCACCCGACGGCTCT4040.15215483637705776No Hit
CGAGTAGGGGTCTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCGTTCCGC4000.15064835284857203No Hit
CGAGTAGGGGACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAGATGTG4000.15064835284857203No Hit
CGAGTAGGGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCATCGACTGCT3970.14951849020220775No Hit
CGAGTAGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCC3870.14575228138099344No Hit
CGAGTAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG3800.14311593520614344No Hit
CGAGTAGGGGAATGCTGTACATCTATTTGATTGTAAATTGTGTTTGTGTATT3790.142739314324022No Hit
CGAGTAGGGCAAGCTGATGGTTGAACTGTCCAAATCCCAGGATGATGAAATT3790.142739314324022No Hit
CGAGTAGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA3780.14236269344190058No Hit
CGAGTAGGGGTCAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT3770.14198607255977916No Hit
CGAGTAGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC3770.14198607255977916No Hit
CGAGTAGGGGTCTGTCTGCCTGCCAGTGTGCTACTACTTTCCCAGATGTGTA3750.14123283079553628No Hit
CGAGTAGGGGGGGCAGCAGAGCCATGGAGCCCGGGGCTGCTGAGCTTTATGA3740.14085620991341485No Hit
CGAGTAGGGGGGGAAGGTGCCCGGGCGGCGGCCATTTTGTCTTCTCGGCTCC3670.13821986373856485No Hit
CGAGTAGGGGGGGCTGGAGAGATGGCTCAGGGGTTAAGAGCACTGACTGCTC3660.13784324285644342No Hit
CGAGTAGGGGGGTGGGTCTGGGCCTGGAGAGTGAAAGCGAAAGCTGGGGCTG3650.13746662197432197No Hit
CGAGTAGGGACTCGCTTATGTCCTCCGCGCCACCACGGTCGCCCACCCCGCG3580.13483027579947196No Hit
CGAGTAGGGGATTGACAAGGACATTGGCTCCAAGTCTGACCCACTCTGCGTC3530.13294717138886483No Hit
CGAGTAGGGGCTCGGATGTGGCTGAACCGAAGCGCGCAGGCGGGAGACTCTG3520.1325705505067434No Hit
CGAGTAGGGGAGCTGCGCACTCCTGGACTGGCGACGTTGTGCTTCTAACAGC3450.1299342043318934No Hit
CGAGTAGGGGCTCTCGCCAGGCGTCCTCGTTGGAGTGACATCGTCTTTAAAC3440.12955758344977197No Hit
CGAGTAGGGGGTCCCTCCGGACAGCTGGCTGGCTGGCTTTGAGGACCTTCCA3430.12918096256765052No Hit
CGAGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT3410.12842772080340767No Hit
CGAGTAGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT3350.1261679955106791No Hit
CGAGTAGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT3340.12579137462855766No Hit
CGAGTAGGGGCTTCCGCTTCCGGTCCGCAGAGACCCGGAGTCCGAATTCAGA3340.12579137462855766No Hit
CGAGTAGGGGCTGGTGATATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTC3340.12579137462855766No Hit
CGAGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG3330.12541475374643624No Hit
CGAGTAGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC3320.12503813286431478No Hit
CGAGTAGGGCCATCAGGCCTCTGACCCAGACTGCTGTGTCCCTTCTTCCTGT3320.12503813286431478No Hit
CGAGTAGGGGCTCTTCTCGCTGAGCGCCGCCAACATGGTGTTCAGGCGTTAC3280.12353164933582908No Hit
CGAGTAGGGGGGGCTGGAAAGATGGCTCAGTGGTTAAAAGCATTGACTGCTC3200.12051868227885765No Hit
CGAGTAGGGAGCTATAAACACAGTGGAGCACATGCCCCTGTGGTATGATAGG3170.11938881963249334No Hit
CGAGTAGGGACTTCTTTTCCTTTTTTTTAAAGGAAGTTTTCTTGTTACATGT3160.11901219875037192No Hit
CGAGTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG3150.11863557786825048No Hit
CGAGTAGGGGTGCTCGAGTAGGGGTGCTCGAGTAGGGGGGGTTGGGGATTTA3150.11863557786825048No Hit
CGAGTAGGGGCTCTCTTCTTTTCCGGCTGGAACCATGGAGGCTGTTCCAGAG3130.11788233610400761No Hit
CGAGTAGGGGAAGCAAAGATGTCGGAAGATTCAGAGGTTGTGCTGCAGCTCC3110.11712909433976477No Hit
CGAGTAGGGGGTCACGGCTAGGGTCGAGGTTGCTACGGCGGACCAGCGGTGG3090.11637585257552191No Hit
CGAGTAGGGGTGCTCGAGTAGGGCAAGATGAAAAGCTGAGACTGTCAACCCA3080.11599923169340047No Hit
CGAGTAGGGGGGCTGGAGCGATGGCTCAGTGGTTAAAAGCATTGGCTGCTCT3070.11562261081127904No Hit
CGAGTAGGGGCCTTGCTTCGCTGCGCTTCTCCTGCAGCCTGGTAGGCACCGG3060.1152459899291576No Hit
CGAGTAGGGGCAGGGTTGTTTGTGCAGTCTCGGTGGCGGCGGCGACCCACAG3040.11449274816491474No Hit
CGAGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG3000.11298626463642904No Hit
CGAGTAGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC3000.11298626463642904No Hit
CGAGTAGGGATACATAGATAGCAATGTGCAGGGATTTTTACCCAGCCAGCTA3000.11298626463642904No Hit
CGAGTAGGGGAATTGTGTTCGCAGCCTTCGCCGCGCCGCTGCCGCTCTCCAA2980.11223302287218617No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTC2970.11185640199006473No Hit
CGAGTAGGGAGTCTCAGCTCAGCGCACAGCTGGGCTTGAATTTGGGAGGAGC2960.11147978110794331No Hit
CGAGTAGGGGTTTTTCAGATCCAAGAGAACTCCTCTGAAAATTCTTTTTAAT2960.11147978110794331No Hit
CGAGTAGGGGGAGCAGGTCTGACTCCTGACTGGCTGGAGTGCTTTTGGAGAA2950.11110316022582187No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCTC2940.11072653934370044No Hit
CGAGTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG2920.10997329757945759No Hit
CGAGTAGGGGCTCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAG2900.10922005581521473No Hit
CGAGTAGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA2890.1088434349330933No Hit
CGAGTAGGGTCCCCACTGTGGCCGCGGCGCGGGAAGGACAGTGAGCGGCCGC2880.10846681405097186No Hit
CGAGTAGGGGGTCCGCGCTGGAGACCACAGCTCGGCACAGGCGTCCCGTCCT2870.10809019316885043No Hit
CGAGTAGGGCAAGCAGGAGTACGATGAGTCCGGCCCCTCCATCGTGCACCGC2870.10809019316885043No Hit
CGAGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT2850.10733695140460758No Hit
CGAGTAGGGGGGCTGGAAAGATGGCTCAGTGGTTAAAAGCATTGACTGCTCT2840.10696033052248616No Hit
CGAGTAGGGGATGTGATAAAAGAATTGTATAAAGGGAATAATTGTCTGATAC2840.10696033052248616No Hit
CGAGTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA2840.10696033052248616No Hit
CGAGTAGGGGCAGGGATGAGTCACTCTGGGTGCCGTGCTCAGTTCTCATGTT2820.10620708875824329No Hit
CGAGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC2810.10583046787612185No Hit
CGAGTAGGGGGTGACGGGCAGGAAACCATTACACCACCACCCCGGCTGTGCT2810.10583046787612185No Hit
CGAGTAGGGGGAGTGCTGCAGCTTCCCTGGCCCGCTGCGCAGCGTCGGCCCT2800.10545384699400043No Hit
CGAGTAGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCA2800.10545384699400043No Hit
CGAGTAGGGGACTGGAGAGATGGCTCAGTGGTTAAAAGCACTCACTGGCTGC2800.10545384699400043No Hit
CGAGTAGGGGGTCCCCCTCGCCACCAGCGGCAAACTAGGACTGTGCGTTTGC2800.10545384699400043No Hit
CGAGTAGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT2770.10432398434763612No Hit
CGAGTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA2740.10319412170127186No Hit
CGAGTAGGGGGGTTCGGCCATCTTGACTCCCTGACCTTGGACTGGTCCTCGT2730.10281750081915042No Hit
CGAGTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGGCTGTTTG2730.10281750081915042No Hit
CGAGTAGGGGGGAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC2720.102440879937029No Hit
CGAGTAGGGGACTTCCGGCGGTGGCGATTTGGAGGACTGAAGGAAGATGGGT2700.10168763817278613No Hit
CGAGTAGGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTAGCG2690.10131101729066469No Hit
CGAGTAGGGACGCTCAGGAGCAGCCAAGCGGTAGAAGCCGGGTGGCATGGCA2670.10055777552642185No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT206.302644E-446.00000428
CGGGTTA206.302644E-446.00000441
AAACTGA206.302644E-446.00000410
TTCTACG206.302644E-446.00000421
CGGCGTA351.01559635E-746.00000434
TCGTTTA206.302644E-446.00000425
ACTATAC206.302644E-446.00000440
TGATAAG206.302644E-446.00000413
GTCGCAA351.01559635E-746.00000444
TCCATAC206.302644E-446.00000421
TCGCAAT351.01559635E-746.00000445
ATGATTG206.302644E-446.00000420
ACTGCAT206.302644E-446.00000431
ATCTATC206.302644E-446.00000442
ATGATAG405.5824785E-946.00000445
GTATTCG206.302644E-446.00000424
GATAACT206.302644E-446.0000049
CAAAATT206.302644E-446.00000432
ATAGGGC206.302644E-446.00000436
CGAGTAT206.302644E-446.0000041
Produced by FastQC (version 0.11.5)