FastQCFastQC Report
Fri 17 Jun 2016
SRR1527579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943786
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA49170.5209867491147357No Hit
TGCAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC47740.5058350092075957No Hit
TGCAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC47110.49915976715060406No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC42690.45232711652853513No Hit
TGCAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA30940.3278285543544829No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA29060.30790878440663455No Hit
TGCAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC23650.2505864676950071No Hit
TGCAATGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG22750.24105040761359034No Hit
TGCAATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC21220.22483910547518188No Hit
TGCAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT18910.20036321793287884No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG18580.1968666625696927No Hit
TGCAATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT16080.17037760678797947No Hit
TGCAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT15690.16624531408603221No Hit
TGCAATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA15130.16031176559092847No Hit
TGCAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG14940.15829859735151824No Hit
TGCAATGGGGTGCCAGGCGGTGGTGGCGCACGCCTTTAATCCCAGCACTT14460.1532126986414293No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG14350.15204718018703392No Hit
TGCAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG13050.13827287118054304No Hit
TGCAATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA12280.13011424199977537No Hit
TGCAATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCT12190.1291606359916337No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCT11270.11941266346396323No Hit
TGCAATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT11040.11697567033204562No Hit
TGCAATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT10740.11379698363824002No Hit
TGCAATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGC10690.11326720252260575No Hit
TGCAATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC10500.11125403428319555No Hit
TGCAATGGGGGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAGTATCGGTG10420.11040638449818074No Hit
TGCAATGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCCCAGCACTTGG10340.1095587347131659No Hit
TGCAATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC9820.10404901111056956No Hit
TGCAATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTG9510.10076436819363713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA207.8557647E-444.00000431
CGCATAA207.8557647E-444.00000444
ACCGAAT207.8557647E-444.00000420
CGTTACG207.8557647E-444.00000444
AACGGTA408.309144E-944.00000444
ACGTGCG207.8557647E-444.00000438
GCGATTA408.309144E-944.00000423
AATTCGT207.8557647E-444.00000413
CAAGCCG750.044.00000426
ATGCGAA207.8557647E-444.00000418
TTACGAA207.8557647E-444.00000427
CTTTCGT207.8557647E-444.00000411
ACGTAAT408.309144E-944.00000421
ATCGCGC207.8557647E-444.00000427
GTCGGTA650.044.00000410
GTACGTT207.8557647E-444.00000419
AATCGCT207.8557647E-444.00000426
GTACGGT207.8557647E-444.00000414
ACGAATC207.8557647E-444.00000420
TGCCGAT207.8557647E-444.00000413
Produced by FastQC (version 0.11.5)