##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527579_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	943786
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63207231300316	31.0	31.0	34.0	30.0	34.0
2	31.86719870818173	33.0	31.0	34.0	30.0	34.0
3	32.552161189083115	34.0	31.0	34.0	31.0	34.0
4	35.37596234739655	37.0	35.0	37.0	33.0	37.0
5	35.62711038307413	37.0	35.0	37.0	35.0	37.0
6	35.876141413413634	37.0	35.0	37.0	35.0	37.0
7	36.48171407501277	37.0	37.0	37.0	35.0	37.0
8	36.67692993962614	37.0	37.0	37.0	35.0	37.0
9	38.6571426149572	39.0	39.0	39.0	39.0	39.0
10	37.40102523241497	39.0	37.0	39.0	34.0	39.0
11	37.370118861691104	39.0	37.0	39.0	35.0	39.0
12	37.20525839544134	39.0	37.0	39.0	35.0	39.0
13	37.197365716380624	39.0	37.0	39.0	34.0	39.0
14	38.04373766934453	40.0	38.0	40.0	34.0	40.0
15	38.00920123841634	40.0	38.0	40.0	34.0	40.0
16	38.0900585514089	40.0	38.0	40.0	35.0	40.0
17	38.013660935847746	40.0	38.0	40.0	34.0	40.0
18	37.87192011748426	40.0	37.0	40.0	34.0	40.0
19	37.80572608621022	40.0	37.0	40.0	34.0	40.0
20	37.64842665604279	39.0	37.0	40.0	33.0	40.0
21	37.5015384843598	39.0	36.0	40.0	33.0	40.0
22	37.34570442875821	39.0	36.0	40.0	33.0	40.0
23	37.13294221359503	38.0	36.0	40.0	33.0	40.0
24	36.97426111427802	38.0	36.0	40.0	33.0	40.0
25	36.745956180744365	38.0	35.0	40.0	32.0	40.0
26	36.168070939810505	38.0	35.0	40.0	31.0	40.0
27	36.07743386742334	38.0	35.0	39.0	31.0	40.0
28	35.92099480178769	38.0	34.0	39.0	31.0	40.0
29	35.61237081287495	37.0	34.0	39.0	31.0	40.0
30	35.36183308504258	37.0	34.0	38.0	30.0	40.0
31	35.224725732316436	37.0	34.0	38.0	30.0	40.0
32	35.10096780414204	36.0	34.0	38.0	30.0	40.0
33	34.52352757934532	36.0	33.0	38.0	29.0	39.0
34	34.032922717649974	36.0	32.0	38.0	27.0	39.0
35	33.909345974617125	35.0	32.0	38.0	27.0	39.0
36	33.62726825784659	35.0	32.0	38.0	27.0	39.0
37	33.49558692330677	35.0	32.0	38.0	27.0	39.0
38	32.951325830219986	34.0	31.0	37.0	26.0	38.0
39	32.16447160691089	34.0	30.0	37.0	24.0	38.0
40	31.88876715696143	33.0	30.0	36.0	24.0	38.0
41	31.613114625561302	33.0	29.0	36.0	23.0	38.0
42	31.29195813457712	33.0	29.0	36.0	23.0	38.0
43	30.714801872458374	32.0	28.0	35.0	23.0	38.0
44	30.352678467364424	32.0	28.0	35.0	22.0	37.0
45	29.97115023956702	31.0	27.0	34.0	21.0	37.0
46	29.70275359032662	31.0	27.0	34.0	21.0	37.0
47	29.272978196328406	31.0	26.0	34.0	20.0	37.0
48	28.741137291716555	31.0	26.0	34.0	19.0	36.0
49	28.197782124337508	30.0	25.0	33.0	18.0	36.0
50	31.745398851010716	33.0	30.0	36.0	24.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	12.0
15	21.0
16	54.0
17	150.0
18	255.0
19	407.0
20	656.0
21	961.0
22	1543.0
23	2269.0
24	3308.0
25	4724.0
26	6711.0
27	9267.0
28	12588.0
29	17424.0
30	24006.0
31	33628.0
32	52516.0
33	82515.0
34	120261.0
35	227609.0
36	281030.0
37	60569.0
38	1294.0
39	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.56102336758545	0.2765457423610861	0.13477631581735688	0.027654574236108606
8	99.24612147245244	0.5583892958785148	0.1572390351202497	0.0382501965487939
9	97.71166344912936	1.4605005795805406	0.25291750460379786	0.5749184666863039
10	53.05715490587909	35.84329498424431	4.403540633152007	6.696009476724596
11	33.37250181714923	22.507856653944856	23.52185770926884	20.597783819637076
12	28.78586883043402	16.93402953635676	28.7719885652044	25.508113068004825
13	25.426950601089654	16.63290195023024	30.368113110387313	27.572034338292788
14	27.236576935873174	17.810817282731467	29.07195063287652	25.880655148518837
15	25.861371115909748	20.139735067059693	27.43248999243473	26.56640382459583
16	30.02248391054752	23.260463706814893	23.110217782421014	23.606834600216576
17	30.955852280071966	22.156823686725595	22.673466230692128	24.213857802510315
18	30.567628678535176	20.360124011163546	24.26450487716495	24.807742433136326
19	30.994526301513265	21.819988853405327	21.549270703316218	25.63621414176519
20	31.66056712008866	20.976577317315577	23.677931225934692	23.684924336661066
21	32.015838336233	20.071711171812254	25.316226347922093	22.59622414403265
22	33.3412447313268	19.465323706857273	23.02820766572083	24.16522389609509
23	30.92353563201827	21.869152540936188	23.64985282680608	23.55745900023946
24	29.790757650569088	21.14218689406285	22.525445386983915	26.54161006838415
25	28.336614444376163	21.46726058661603	22.782919009182166	27.41320595982564
26	27.892763825697774	22.147711451536683	22.63330882212705	27.32621590063849
27	27.616112127113563	20.912261889877577	22.19327262748123	29.27835335552763
28	28.420001991976996	22.573549512283506	22.443859095176236	26.56258940056326
29	30.52842487597824	21.59981182174773	21.68023259510101	26.191530707173023
30	28.379632670965666	21.870529971836834	21.487180356563883	28.262657000633617
31	29.941851224747985	21.748468402794703	21.150133610797365	27.159546761659954
32	33.02644879241692	21.751435177042254	21.917998359797668	23.304117670743153
33	31.5727294111165	21.685106581364842	23.438046336775496	23.304117670743153
34	28.55287109577807	23.208227288813355	26.467864537087856	21.77103707832072
35	28.37168595423115	25.043495029593572	24.81802018677963	21.766798829395647
36	28.760757205552956	24.96010748199274	22.798388617758686	23.48074669469562
37	29.54207839489037	26.12064599390116	20.806411622973854	23.53086398823462
38	30.633109624427572	25.070195997821543	21.224832748101793	23.071861629649092
39	30.007226214417255	23.593060291210083	21.51653023037002	24.883183264002646
40	28.094292562085048	23.825210376081017	22.667850550866405	25.41264651096753
41	26.886391618438925	22.817990519037153	23.821184039602198	26.47443382292172
42	27.09745641490762	22.11719605927615	22.482639072840666	28.302708452975565
43	25.516589565854968	22.324128563042894	24.931923126641	27.22735874446114
44	27.733511622338114	22.298169288376815	25.094248060471337	24.874071028813734
45	27.617383601791083	21.4667308055004	24.204215786205772	26.711669806502748
46	26.457586783444555	23.0750403163429	24.483092565475648	25.984280334736898
47	27.5081427357473	24.216824576757865	23.498653296404058	24.776379391090778
48	28.209996757739574	22.946621373913153	24.597419330229524	24.24596253811775
49	26.972004246725422	22.9834941395613	24.074525369098502	25.969976244614774
50	26.832777769536737	23.32488509047602	23.914425515953827	25.927911624033413
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	10.0
4	20.0
5	18.5
6	17.0
7	28.5
8	40.0
9	226.5
10	413.0
11	799.0
12	1185.0
13	1174.0
14	1163.0
15	1078.5
16	994.0
17	1263.5
18	1533.0
19	1448.5
20	1364.0
21	1819.0
22	2274.0
23	2511.0
24	2748.0
25	3994.0
26	5240.0
27	4943.5
28	4647.0
29	6271.0
30	7895.0
31	9430.5
32	10966.0
33	11803.5
34	12641.0
35	14584.5
36	16528.0
37	17735.5
38	18943.0
39	21735.5
40	24528.0
41	25627.5
42	26727.0
43	29900.5
44	33074.0
45	35024.5
46	36975.0
47	39291.5
48	41608.0
49	45359.5
50	49111.0
51	51711.5
52	54312.0
53	56940.5
54	59569.0
55	71536.0
56	83503.0
57	86799.0
58	90095.0
59	86041.5
60	81988.0
61	82609.0
62	83230.0
63	73098.5
64	62967.0
65	51327.5
66	39688.0
67	32709.5
68	25731.0
69	23101.0
70	20471.0
71	17030.0
72	13589.0
73	16274.0
74	18959.0
75	12586.5
76	6214.0
77	4001.5
78	1789.0
79	1222.5
80	656.0
81	495.0
82	334.0
83	190.5
84	47.0
85	27.5
86	8.0
87	4.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	943786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.655306792281948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.7002130927117	7.533324607825522
2	10.244760954149895	2.1832214196011654
3	3.9098367063086914	1.2498152884033287
4	1.716908498513846	0.7317674714376475
5	0.9289502484243533	0.4949124945864008
6	0.5429709169874885	0.34713130198730063
7	0.4049783081952776	0.30206176826279957
8	0.32798243231516055	0.2795802751037508
9	0.2839847889557897	0.2723350545598844
>10	6.193668249268165	18.72335464307484
>50	3.0268378738351203	22.401476090047353
>100	1.6209131792159663	34.73156288734313
>500	0.07099619723894732	4.836184713017657
>1k	0.026998553879599683	5.913271984749216
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCAATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA	4917	0.5209867491147357	No Hit
TGCAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4774	0.5058350092075957	No Hit
TGCAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4711	0.49915976715060406	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4269	0.45232711652853513	No Hit
TGCAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	3094	0.3278285543544829	No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	2906	0.30790878440663455	No Hit
TGCAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2365	0.2505864676950071	No Hit
TGCAATGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAG	2275	0.24105040761359034	No Hit
TGCAATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	2122	0.22483910547518188	No Hit
TGCAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1891	0.20036321793287884	No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1858	0.1968666625696927	No Hit
TGCAATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1608	0.17037760678797947	No Hit
TGCAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1569	0.16624531408603221	No Hit
TGCAATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1513	0.16031176559092847	No Hit
TGCAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1494	0.15829859735151824	No Hit
TGCAATGGGGTGCCAGGCGGTGGTGGCGCACGCCTTTAATCCCAGCACTT	1446	0.1532126986414293	No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1435	0.15204718018703392	No Hit
TGCAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1305	0.13827287118054304	No Hit
TGCAATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1228	0.13011424199977537	No Hit
TGCAATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCT	1219	0.1291606359916337	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCT	1127	0.11941266346396323	No Hit
TGCAATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1104	0.11697567033204562	No Hit
TGCAATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT	1074	0.11379698363824002	No Hit
TGCAATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGC	1069	0.11326720252260575	No Hit
TGCAATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1050	0.11125403428319555	No Hit
TGCAATGGGGGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAGTATCGGTG	1042	0.11040638449818074	No Hit
TGCAATGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCCCAGCACTTGG	1034	0.1095587347131659	No Hit
TGCAATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	982	0.10404901111056956	No Hit
TGCAATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTG	951	0.10076436819363713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.003178686693805587	0.0	0.0	0.0	0.0
8	0.004768030040708381	0.0	0.0	0.0	0.0
9	0.00582759227197691	0.0	0.0	0.0	0.0
10	0.019813813724721494	0.0	0.0	0.0	0.0
11	0.03528342230124202	0.0	0.0	0.0	0.0
12	0.04619691328330787	0.0	0.0	0.0	0.0
13	0.05371980512531443	0.0	0.0	0.0	0.0
14	0.06262012786797007	0.0	0.0	0.0	0.0
15	0.07258001284189425	0.0	0.0	0.0	0.0
16	0.08391732871646751	0.0	0.0	0.0	0.0
17	0.09440699480602595	0.0	0.0	0.0	0.0
18	0.10129414930927139	0.0	0.0	0.0	0.0
19	0.11040638449818073	0.0	0.0	0.0	0.0
20	0.11898883857145581	0.0	0.0	0.0	0.0
21	0.12651173041346236	0.0	0.0	0.0	0.0
22	0.13350484113983466	0.0	0.0	0.0	0.0
23	0.1415575140974755	0.0	0.0	0.0	0.0
24	0.15204718018703392	0.0	0.0	0.0	0.0
25	0.16084154670656273	0.0	0.0	0.0	0.0
26	0.17069547545736002	0.0	0.0	0.0	0.0
27	0.1816089664394259	0.0	0.0	0.0	0.0
28	0.1988798308091029	0.0	0.0	0.0	0.0
29	0.20693250376674374	0.0	0.0	0.0	0.0
30	0.21678643251754104	0.0	0.0	0.0	0.0
31	0.22875948573087543	0.0	0.0	0.0	0.0
32	0.23924915182043388	0.0	0.0	0.0	0.0
33	0.24846734323247008	0.0	0.0	0.0	0.0
34	0.2583212719832674	0.0	0.0	0.0	0.0
35	0.27400279300604163	0.0	0.0	0.0	0.0
36	0.28682349600439083	0.0	0.0	0.0	0.0
37	0.2987965492177252	0.0	0.0	0.0	0.0
38	0.30949812775353736	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTAA	20	7.8557647E-4	44.000004	31
CGCATAA	20	7.8557647E-4	44.000004	44
ACCGAAT	20	7.8557647E-4	44.000004	20
CGTTACG	20	7.8557647E-4	44.000004	44
AACGGTA	40	8.309144E-9	44.000004	44
ACGTGCG	20	7.8557647E-4	44.000004	38
GCGATTA	40	8.309144E-9	44.000004	23
AATTCGT	20	7.8557647E-4	44.000004	13
CAAGCCG	75	0.0	44.000004	26
ATGCGAA	20	7.8557647E-4	44.000004	18
TTACGAA	20	7.8557647E-4	44.000004	27
CTTTCGT	20	7.8557647E-4	44.000004	11
ACGTAAT	40	8.309144E-9	44.000004	21
ATCGCGC	20	7.8557647E-4	44.000004	27
GTCGGTA	65	0.0	44.000004	10
GTACGTT	20	7.8557647E-4	44.000004	19
AATCGCT	20	7.8557647E-4	44.000004	26
GTACGGT	20	7.8557647E-4	44.000004	14
ACGAATC	20	7.8557647E-4	44.000004	20
TGCCGAT	20	7.8557647E-4	44.000004	13
>>END_MODULE