FastQCFastQC Report
Fri 17 Jun 2016
SRR1527578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734346
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG42370.5769759759023676No Hit
TGCAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT39280.5348977185141609No Hit
TGCAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC38170.5197822279960672No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC32700.44529418012762373No Hit
TGCAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA23220.3161997205676888No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC21320.2903263584196005No Hit
TGCAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC19280.262546538007969No Hit
TGCAATGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGC18510.2520610175584806No Hit
TGCAATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG15110.205761316872428No Hit
TGCAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG14900.20290162947711296No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT14500.19745460586698912No Hit
TGCAATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG13140.17893472559256807No Hit
TGCAATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT12610.171717419309154No Hit
TGCAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG12530.17062801458712923No Hit
TGCAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC12450.16953860986510447No Hit
TGCAATGGGGTGCCAGGCGGTGGTGGCGCACGCCTTTAATCCCAGCACTTGG12200.16613422010877707No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT11610.1580998602838444No Hit
TGCAATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA10100.1375373461556269No Hit
TGCAATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC10020.13644794143360214No Hit
TGCAATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCT9780.13317972726752783No Hit
TGCAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC9390.1278688792476571No Hit
TGCAATGGGGGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAGTATCGGTGGG9260.12609859657436684No Hit
TGCAATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT9080.12364743594981112No Hit
TGCAATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC9070.12351126035955802No Hit
TGCAATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC8560.11656630525665014No Hit
TGCAATGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGA8190.11152780841728559No Hit
TGCAATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8060.10975752574399535No Hit
TGCAATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC7950.10825959425121129No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCTCT7810.10635313598766793No Hit
TGCAATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT7810.10635313598766793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTAC351.0188887E-746.00000424
CCGTACC351.0188887E-746.00000435
ATAAGCG351.0188887E-746.00000439
GCGATTA351.0188887E-746.00000423
AACGTAA301.8604787E-646.041
ACACGTT206.3099584E-446.033
CAAACCG206.3099584E-446.011
CGTATGG206.3099584E-446.013
CTCGTAC206.3099584E-446.028
CTCGTAA206.3099584E-446.040
CGTATAG253.415942E-546.046
TTTACGT206.3099584E-446.012
CAACGAA206.3099584E-446.046
ACGTTAT501.6370905E-1146.022
ACGTTAC253.415942E-546.026
CCGATAT206.3099584E-446.013
GACGTTA206.3099584E-446.09
ACCGCAA301.8604787E-646.035
GACGTAT206.3099584E-446.011
ATTACGA501.6370905E-1146.044
Produced by FastQC (version 0.11.5)