##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527578_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	734346
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23450526046305	33.0	31.0	34.0	31.0	34.0
2	32.518270678944255	34.0	31.0	34.0	31.0	34.0
3	32.95470118990231	34.0	31.0	34.0	31.0	34.0
4	35.907928959918074	37.0	35.0	37.0	35.0	37.0
5	36.09270698008841	37.0	35.0	37.0	35.0	37.0
6	36.30478684434858	37.0	37.0	37.0	35.0	37.0
7	36.6299373864636	37.0	37.0	37.0	35.0	37.0
8	36.73574718184616	37.0	37.0	37.0	36.0	37.0
9	38.750454145593494	39.0	39.0	39.0	39.0	39.0
10	37.78863505758866	39.0	37.0	39.0	35.0	39.0
11	37.85995293771601	39.0	37.0	39.0	35.0	39.0
12	37.709917395886954	39.0	37.0	39.0	35.0	39.0
13	37.7124706882042	39.0	37.0	39.0	35.0	39.0
14	38.4853121008353	40.0	38.0	40.0	35.0	40.0
15	38.5117288035885	40.0	38.0	40.0	35.0	40.0
16	38.603462128206594	40.0	38.0	40.0	35.0	40.0
17	38.56202661960438	40.0	38.0	40.0	35.0	40.0
18	38.479878694784205	40.0	38.0	40.0	35.0	40.0
19	38.39334727771377	40.0	38.0	40.0	35.0	40.0
20	38.35879953046656	40.0	38.0	40.0	35.0	40.0
21	38.27732703657404	40.0	38.0	40.0	34.0	40.0
22	38.282146290713094	40.0	38.0	40.0	35.0	40.0
23	38.129084927268615	40.0	38.0	40.0	34.0	40.0
24	38.0544498097627	40.0	38.0	40.0	34.0	40.0
25	38.10714703967884	40.0	38.0	40.0	35.0	40.0
26	38.082700797716605	40.0	38.0	40.0	35.0	40.0
27	38.02419567887617	40.0	38.0	40.0	35.0	40.0
28	37.93643051095805	40.0	38.0	40.0	34.0	40.0
29	37.89442851190039	40.0	38.0	40.0	34.0	40.0
30	37.806712367194756	40.0	37.0	40.0	34.0	40.0
31	37.80882853586729	40.0	37.0	40.0	34.0	40.0
32	37.67056809732742	40.0	37.0	40.0	34.0	40.0
33	37.482997115801	40.0	37.0	40.0	33.0	40.0
34	37.080530158807974	39.0	36.0	40.0	32.0	40.0
35	36.99984612158301	39.0	35.0	40.0	32.0	40.0
36	36.983637141075185	39.0	35.0	40.0	32.0	40.0
37	36.90700432766026	39.0	35.0	40.0	32.0	40.0
38	37.002688106151595	39.0	35.0	40.0	32.0	40.0
39	36.98968333728243	39.0	35.0	40.0	32.0	40.0
40	36.82483461474564	39.0	35.0	40.0	32.0	40.0
41	36.77941324661672	39.0	35.0	40.0	32.0	40.0
42	36.62105601446729	39.0	35.0	40.0	32.0	40.0
43	36.51124129497539	39.0	35.0	40.0	32.0	40.0
44	36.45616643925343	38.0	35.0	40.0	31.0	40.0
45	36.362720025709955	38.0	35.0	40.0	31.0	40.0
46	36.17527432572656	38.0	35.0	40.0	31.0	40.0
47	36.11340566980687	38.0	35.0	40.0	31.0	40.0
48	35.84594591650257	38.0	35.0	40.0	30.0	40.0
49	35.67958155964627	37.0	34.0	40.0	30.0	40.0
50	36.019631073090885	38.0	35.0	40.0	31.0	40.0
51	36.118301182276475	38.0	35.0	40.0	32.0	40.0
52	35.74908558091145	37.0	35.0	40.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	8.0
14	7.0
15	15.0
16	17.0
17	42.0
18	77.0
19	145.0
20	228.0
21	397.0
22	631.0
23	959.0
24	1267.0
25	1845.0
26	2359.0
27	3248.0
28	4290.0
29	5646.0
30	7249.0
31	9998.0
32	14043.0
33	21536.0
34	44141.0
35	49558.0
36	74908.0
37	126597.0
38	279970.0
39	85162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.57894507493744	0.27412146317948216	0.11670248084690324	0.0302309810361873
8	99.25253218510076	0.563358416877058	0.1482952177856215	0.035814180236564234
9	97.68705759955117	1.4805010172316593	0.2357199467281091	0.5967214364890665
10	53.17711269619498	35.6938010147805	4.416855269859167	6.712231019165353
11	33.42280069612962	22.41790654541592	23.423971806205792	20.735320952248667
12	28.620023803493176	16.8506398890986	28.82905333453168	25.700282972876547
13	25.484853189096146	16.621320195112386	30.344142951687626	27.549683664103842
14	27.499434871300448	17.75293935011561	28.916205712293657	25.831420066290278
15	25.741135649952472	20.076231095423683	27.42957679350061	26.753056461123233
16	30.223082851952622	23.234960086934496	22.953893668652107	23.588063392460775
17	30.99982841875628	22.065347942250654	22.64613683468011	24.288686804312952
18	30.666062047045944	20.12675223940758	24.368621875791522	24.838563837754954
19	31.16691586799683	21.72763247842298	21.517785893842955	25.587665759737238
20	31.823146037426497	20.75833462700144	23.633409864015057	23.785109471557004
21	32.12354938952483	20.016586186892827	25.303194951698515	22.556669471883826
22	33.6882069215329	19.22336337366854	22.99488252131829	24.093547183480265
23	31.072410008361178	21.534671667034342	23.5583771137856	23.834541210818877
24	29.760085845092092	21.07712168378394	22.59861155368178	26.564180917442187
25	28.540088732014606	21.33313179345976	22.780542142259915	27.346237332265716
26	27.957257205731356	21.902209585127448	22.601607416667346	27.538925792473844
27	27.645687455232277	20.831052392196593	22.203021463996535	29.320238688574594
28	28.4518469495306	22.437107303641607	22.458895398082106	26.652150348745685
29	30.648359220313043	21.384061464214415	21.764536063381566	26.203043252090975
30	28.379810062286715	21.670847257287438	21.603985042473166	28.345357637952684
31	29.988043783175776	21.55005950873294	21.219016648827665	27.24288005926362
32	33.13138493298799	21.638573642397454	21.89308582058049	23.336955604034067
33	31.538266702617022	21.427092950734394	23.609034433359753	23.42560591328883
34	28.773085166937655	23.072911134533314	26.359372829701535	21.7946308688275
35	28.489431412440457	24.765846072559803	24.93552085801516	21.80920165698458
36	28.92410389652834	24.691630375871863	22.82684184294597	23.55742388465383
37	29.712288212913258	26.018252976117523	20.683846579132997	23.58561223183622
38	30.620987926672168	25.117996148954308	21.25973315031334	23.001282774060183
39	29.980962652482617	23.38870232833024	21.690456542283883	24.939878476903257
40	28.072461755085477	23.806761390407246	22.75303467302879	25.367742181478487
41	27.048285140791943	22.56211649549395	23.934357918474397	26.45524044523971
42	27.39743935420088	21.818461597121793	22.525076734945106	28.259022313732217
43	25.515492696903095	22.13561454682125	24.996118995677787	27.352773760597866
44	27.893118502722146	22.086863685510643	25.154082680371374	24.865935131395826
45	27.85703197130508	21.25047321017613	24.162860558919093	26.7296342595997
46	26.58705841660471	22.89070819477467	24.49921426684424	26.02301912177638
47	27.643917172558986	24.04520484894042	23.61597938846266	24.69489859003794
48	28.546216633575998	22.601607416667346	24.70252442309211	24.14965152666454
49	27.10030421626863	22.651992385060993	24.36535366162545	25.882349737044937
50	26.896313182069488	23.252799089257653	23.986649345131585	25.86423838354127
51	26.90924986314353	22.749766458862716	24.203985587175527	26.136998090818224
52	27.79765941395473	22.439966991036922	23.75365291020854	26.00872068479981
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	5.0
4	10.0
5	11.0
6	12.0
7	23.5
8	35.0
9	161.5
10	288.0
11	663.5
12	1039.0
13	1030.5
14	959.0
15	896.0
16	967.5
17	1039.0
18	1035.5
19	1032.0
20	1162.0
21	1292.0
22	1489.5
23	1687.0
24	2271.0
25	2855.0
26	3518.5
27	4182.0
28	4136.5
29	4091.0
30	5713.0
31	7335.0
32	7537.5
33	7740.0
34	8851.0
35	9962.0
36	11571.5
37	13181.0
38	14478.0
39	16435.5
40	17096.0
41	20719.0
42	24342.0
43	23564.0
44	22786.0
45	25166.5
46	27547.0
47	29467.5
48	31388.0
49	33678.5
50	35969.0
51	37287.5
52	38606.0
53	42346.0
54	46086.0
55	49919.0
56	53752.0
57	63368.5
58	72985.0
59	66898.0
60	60811.0
61	57755.5
62	54700.0
63	56618.0
64	47831.0
65	37126.0
66	29797.0
67	22468.0
68	19737.5
69	17007.0
70	14998.5
71	12990.0
72	14276.0
73	15562.0
74	11389.0
75	7216.0
76	4937.0
77	2658.0
78	1622.0
79	586.0
80	500.5
81	415.0
82	279.5
83	144.0
84	112.5
85	81.0
86	48.0
87	15.0
88	8.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	734346.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.732605610979022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.69319024499447	6.588311232035035
2	9.893523247191169	1.925795197359283
3	3.4629430118509608	1.0111037576292374
4	1.641225112283304	0.6389358694675262
5	0.8898714163786711	0.43303837700484515
6	0.6072392998558855	0.3546012370190618
7	0.4813140994249416	0.32791082132945504
8	0.3931664591232808	0.3061227268889597
9	0.3917672902296036	0.34316248743780176
>10	9.556323543814974	25.64812772180961
>50	3.240475157756293	21.678336914751355
>100	1.669208490156847	32.21505938617491
>500	0.053168417959731915	3.4362548444466237
>1k	0.026584208979865957	5.093239426646295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCAATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	4237	0.5769759759023676	No Hit
TGCAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3928	0.5348977185141609	No Hit
TGCAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3817	0.5197822279960672	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3270	0.44529418012762373	No Hit
TGCAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2322	0.3161997205676888	No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	2132	0.2903263584196005	No Hit
TGCAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1928	0.262546538007969	No Hit
TGCAATGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGC	1851	0.2520610175584806	No Hit
TGCAATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	1511	0.205761316872428	No Hit
TGCAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1490	0.20290162947711296	No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1450	0.19745460586698912	No Hit
TGCAATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1314	0.17893472559256807	No Hit
TGCAATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1261	0.171717419309154	No Hit
TGCAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1253	0.17062801458712923	No Hit
TGCAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1245	0.16953860986510447	No Hit
TGCAATGGGGTGCCAGGCGGTGGTGGCGCACGCCTTTAATCCCAGCACTTGG	1220	0.16613422010877707	No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1161	0.1580998602838444	No Hit
TGCAATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1010	0.1375373461556269	No Hit
TGCAATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1002	0.13644794143360214	No Hit
TGCAATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCT	978	0.13317972726752783	No Hit
TGCAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	939	0.1278688792476571	No Hit
TGCAATGGGGGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAGTATCGGTGGG	926	0.12609859657436684	No Hit
TGCAATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	908	0.12364743594981112	No Hit
TGCAATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	907	0.12351126035955802	No Hit
TGCAATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	856	0.11656630525665014	No Hit
TGCAATGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGA	819	0.11152780841728559	No Hit
TGCAATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	806	0.10975752574399535	No Hit
TGCAATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC	795	0.10825959425121129	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCTCT	781	0.10635313598766793	No Hit
TGCAATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT	781	0.10635313598766793	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.004493794478352167	0.0	0.0	0.0	0.0
8	0.005447023610123838	0.0	0.0	0.0	0.0
9	0.005991725971136222	0.0	0.0	0.0	0.0
10	0.02151574325998916	0.0	0.0	0.0	0.0
11	0.03881004322213235	0.0	0.0	0.0	0.0
12	0.04902321249111454	0.0	0.0	0.0	0.0
13	0.05746609908680649	0.0	0.0	0.0	0.0
14	0.06713456599477631	0.0	0.0	0.0	0.0
15	0.0773477352637585	0.0	0.0	0.0	0.0
16	0.09028441633780261	0.0	0.0	0.0	0.0
17	0.10281257064108744	0.0	0.0	0.0	0.0
18	0.11030222810500773	0.0	0.0	0.0	0.0
19	0.12024304619348372	0.0	0.0	0.0	0.0
20	0.12936681074044115	0.0	0.0	0.0	0.0
21	0.1420311406339791	0.0	0.0	0.0	0.0
22	0.15061020281992413	0.0	0.0	0.0	0.0
23	0.15987014295713464	0.0	0.0	0.0	0.0
24	0.16872155632358588	0.0	0.0	0.0	0.0
25	0.17743679409978402	0.0	0.0	0.0	0.0
26	0.18628820746623526	0.0	0.0	0.0	0.0
27	0.19881636176952008	0.0	0.0	0.0	0.0
28	0.21379567669736063	0.0	0.0	0.0	0.0
29	0.21992357825874997	0.0	0.0	0.0	0.0
30	0.23027292311798525	0.0	0.0	0.0	0.0
31	0.24157549710899223	0.0	0.0	0.0	0.0
32	0.2530142466902523	0.0	0.0	0.0	0.0
33	0.26172948446645045	0.0	0.0	0.0	0.0
34	0.2722150049159388	0.0	0.0	0.0	0.0
35	0.2861049151217546	0.0	0.0	0.0	0.0
36	0.30026717650807655	0.0	0.0	0.0	0.0
37	0.3130676819918676	0.0	0.0	0.0	0.0
38	0.3227361488998374	0.0	0.0	0.0	0.0
39	0.33185991344679483	0.0	0.0	0.0	0.0
40	0.34193690712552394	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTAC	35	1.0188887E-7	46.000004	24
CCGTACC	35	1.0188887E-7	46.000004	35
ATAAGCG	35	1.0188887E-7	46.000004	39
GCGATTA	35	1.0188887E-7	46.000004	23
AACGTAA	30	1.8604787E-6	46.0	41
ACACGTT	20	6.3099584E-4	46.0	33
CAAACCG	20	6.3099584E-4	46.0	11
CGTATGG	20	6.3099584E-4	46.0	13
CTCGTAC	20	6.3099584E-4	46.0	28
CTCGTAA	20	6.3099584E-4	46.0	40
CGTATAG	25	3.415942E-5	46.0	46
TTTACGT	20	6.3099584E-4	46.0	12
CAACGAA	20	6.3099584E-4	46.0	46
ACGTTAT	50	1.6370905E-11	46.0	22
ACGTTAC	25	3.415942E-5	46.0	26
CCGATAT	20	6.3099584E-4	46.0	13
GACGTTA	20	6.3099584E-4	46.0	9
ACCGCAA	30	1.8604787E-6	46.0	35
GACGTAT	20	6.3099584E-4	46.0	11
ATTACGA	50	1.6370905E-11	46.0	44
>>END_MODULE