FastQCFastQC Report
Fri 17 Jun 2016
SRR1527577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012106
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT54680.5402596170756818No Hit
TGCAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC53950.533046933819185No Hit
TGCAATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG51820.5120017073310503No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC45900.45350981023726766No Hit
TGCAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA32210.3182472982078952No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC30620.3025374812519637No Hit
TGCAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC27320.26993220077738894No Hit
TGCAATGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGC22250.21983863350281493No Hit
TGCAATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG21070.2081797756361488No Hit
TGCAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG20840.20590728639095116No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT20390.20146111178078185No Hit
TGCAATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT17090.16885583130620704No Hit
TGCAATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG16730.16529889161807163No Hit
TGCAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG16670.16470606833671572No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT15570.15383764151185744No Hit
TGCAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15300.15116993674575588No Hit
TGCAATGGGGTGCCAGGCGGTGGTGGCGCACGCCTTTAATCCCAGCACTTGG15230.150478309584174No Hit
TGCAATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA14560.14385844960903305No Hit
TGCAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC14150.13980749051976768No Hit
TGCAATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCT13270.13111274905988107No Hit
TGCAATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC12460.12310963476157635No Hit
TGCAATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT11810.11668738254688737No Hit
TGCAATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC11380.11243881569717007No Hit
TGCAATGGGGGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAGTATCGGTGGG11380.11243881569717007No Hit
TGCAATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC11360.1122412079367181No Hit
TGCAATGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGA11320.11184599241581415No Hit
TGCAATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC11160.11026513033219841No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCTCT11010.10878307212880865No Hit
TGCAATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG10560.10433689751863935No Hit
TGCAATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT10320.10196560439321573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG206.311095E-446.019
AACGTTA253.4168654E-546.039
AACCGTT551.8189894E-1246.032
GATACGG405.6097633E-946.011
TCGTTAG253.4168654E-546.020
CTCGTTA253.4168654E-546.019
CTCGTAA206.311095E-446.040
CTCAAAT301.8611809E-646.023
CGAAATA405.6097633E-946.020
ACGTTAT206.311095E-446.022
TAGACGC206.311095E-446.045
GACGTTA501.6370905E-1146.09
GGCGTAA206.311095E-446.031
ACCGAAT253.4168654E-546.020
GATTTCG253.4168654E-546.038
CCTCGAT253.4168654E-546.040
ATCGTGA206.311095E-446.019
CGTTATC206.311095E-446.023
CGTTAGC206.311095E-446.040
GTTACGC501.6370905E-1146.012
Produced by FastQC (version 0.11.5)