##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1012106
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28259490606715	31.0	31.0	33.0	30.0	34.0
2	31.570638846128766	31.0	31.0	34.0	30.0	34.0
3	32.25519164988648	34.0	31.0	34.0	30.0	34.0
4	35.06115762578228	35.0	35.0	37.0	33.0	37.0
5	35.340943537534606	37.0	35.0	37.0	33.0	37.0
6	35.732704874785846	37.0	35.0	37.0	35.0	37.0
7	36.37546264916916	37.0	36.0	37.0	35.0	37.0
8	36.571658502172696	37.0	37.0	37.0	35.0	37.0
9	38.63588892863	39.0	39.0	39.0	38.0	39.0
10	37.19708805204198	39.0	37.0	39.0	34.0	39.0
11	37.186427113365596	39.0	37.0	39.0	34.0	39.0
12	37.047667932015024	39.0	37.0	39.0	34.0	39.0
13	37.018447672476995	39.0	37.0	39.0	33.0	39.0
14	37.78102096025515	40.0	37.0	40.0	33.0	40.0
15	37.81188037616613	40.0	37.0	40.0	33.0	40.0
16	37.87334528201592	40.0	37.0	40.0	33.0	40.0
17	37.86932692820712	40.0	37.0	40.0	33.0	40.0
18	37.80337929031149	40.0	37.0	40.0	33.0	40.0
19	37.678006058653935	40.0	37.0	40.0	33.0	40.0
20	37.59059525385681	40.0	37.0	40.0	33.0	40.0
21	37.593563322418795	40.0	37.0	40.0	33.0	40.0
22	37.60582784807125	40.0	37.0	40.0	33.0	40.0
23	37.534639652368426	39.0	37.0	40.0	33.0	40.0
24	37.51694091330355	39.0	37.0	40.0	33.0	40.0
25	37.52207575095889	39.0	37.0	40.0	33.0	40.0
26	37.45919893766068	39.0	36.0	40.0	33.0	40.0
27	37.3965562895586	39.0	36.0	40.0	33.0	40.0
28	37.243028892230654	39.0	36.0	40.0	33.0	40.0
29	37.22689224251215	39.0	36.0	40.0	33.0	40.0
30	37.16620591123855	39.0	36.0	40.0	32.0	40.0
31	37.12888274548318	39.0	36.0	40.0	32.0	40.0
32	36.98501342744732	39.0	36.0	40.0	32.0	40.0
33	36.85359142224233	39.0	35.0	40.0	32.0	40.0
34	36.538119525029984	39.0	35.0	40.0	31.0	40.0
35	36.44686030909806	38.0	35.0	40.0	31.0	40.0
36	36.45678812298316	38.0	35.0	40.0	31.0	40.0
37	36.426327874748296	38.0	35.0	40.0	31.0	40.0
38	36.27503542119106	38.0	35.0	40.0	31.0	40.0
39	36.19314281310456	38.0	35.0	40.0	31.0	40.0
40	36.41027718440559	38.0	35.0	40.0	31.0	40.0
41	36.53534708815084	39.0	35.0	40.0	31.0	40.0
42	36.55346179155148	39.0	35.0	40.0	32.0	40.0
43	36.47179149219548	38.0	35.0	40.0	31.0	40.0
44	36.41406532517345	38.0	35.0	40.0	31.0	40.0
45	36.32652607533203	38.0	35.0	40.0	31.0	40.0
46	36.048897052285035	38.0	35.0	40.0	31.0	40.0
47	36.04073683981717	38.0	35.0	40.0	31.0	40.0
48	35.89838712545919	37.0	35.0	40.0	31.0	40.0
49	35.78341201415662	37.0	35.0	40.0	31.0	40.0
50	35.61923355854031	37.0	34.0	40.0	30.0	40.0
51	35.4676456813812	37.0	34.0	40.0	30.0	40.0
52	35.02045734340079	36.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	6.0
15	9.0
16	28.0
17	59.0
18	125.0
19	242.0
20	405.0
21	655.0
22	1076.0
23	1656.0
24	2528.0
25	3491.0
26	4805.0
27	6714.0
28	9233.0
29	11972.0
30	15943.0
31	20975.0
32	28716.0
33	42190.0
34	73177.0
35	77665.0
36	117208.0
37	192009.0
38	335989.0
39	65228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.59688016867798	0.2709202395796488	0.10581895572202911	0.026380636020337793
8	99.27319865705766	0.5588347465581668	0.13308882666440075	0.03487776971977243
9	97.7197052482645	1.466743601954736	0.23288074569264486	0.5806704040881094
10	53.07556718367443	35.77807067639161	4.365649447785113	6.780712692148846
11	33.27210786221996	22.5017932904261	23.620747233985373	20.605351613368562
12	28.59868432753091	16.89388265655969	28.834035170229207	25.673397845680196
13	25.265239016466655	16.701412697879473	30.307201024398626	27.726147261255246
14	27.227681685515154	17.780449873827443	29.015340290443888	25.97652815021351
15	25.74631510928697	20.06548721181378	27.478050718007797	26.710146960891446
16	30.125994708064173	23.245490096887085	22.98899522382043	23.63951997122831
17	30.928282215499163	22.038501896046462	22.717976180360555	24.315239708093817
18	30.43663410749467	20.164389895919992	24.39309716571189	25.005878830873446
19	30.870778357207644	21.811549383167375	21.513556880405808	25.804115379219173
20	31.506778934222307	20.993156843255548	23.726269778066726	23.773794444455422
21	31.98449569511494	19.948997437027348	25.308613919885865	22.757892947971854
22	33.41211296050018	19.315466957018337	23.00450743301591	24.26791264946557
23	30.93124633190595	21.692787119135744	23.652463279537912	23.723503269420394
24	29.793717258864188	21.12940739408718	22.48707151227243	26.5898038347762
25	28.38665120056595	21.40862715960581	22.86005616012552	27.34466547970272
26	27.801040602466543	21.998980343956067	22.73161111583174	27.468367937745654
27	27.586142162975026	20.892179277664592	22.22968740428374	29.29199115507664
28	28.566968281978372	22.47966122125548	22.469682029352654	26.48368846741349
29	30.533165498475455	21.4701819769866	21.817971635382065	26.17868088915588
30	28.294763591955785	21.766988833185458	21.581237538360607	28.357010036498153
31	30.09783560219977	21.749994565786587	21.051945151990008	27.100224680023633
32	33.125384100084375	21.657316526134615	21.872313769506356	23.34498560427465
33	31.59886415059292	21.47561619039903	23.48350864435148	23.442011014656565
34	28.669823121293618	23.24311880376166	26.3141410089457	21.772917065999014
35	28.375091146579507	24.964084789537853	24.856289756211307	21.804534307671332
36	28.838184933198697	24.792857665106226	22.725584079137956	23.643373322557125
37	29.611819315368155	25.959533882814647	20.737551205110925	23.691095596706273
38	30.704096211266407	24.95953981104746	21.318221609199036	23.018142368487098
39	30.009504933277743	23.415827986396682	21.534305695253263	25.040361385072313
40	28.08184123006879	23.756800177056554	22.765303238988803	25.39605535388586
41	26.98096839659087	22.619567515655476	23.837720554961635	26.561743532792022
42	27.027307416416857	21.99473177710635	22.652568011650953	28.325392794825838
43	25.497230527237264	22.025854999377536	25.09687720456158	27.38003726882362
44	27.790863802803262	22.09689498926002	25.245577044301683	24.866664163635036
45	27.686032885883492	21.296879971070222	24.311583964525454	26.70550317852083
46	26.647702908588627	22.96014449079444	24.453861552050874	25.938291048566057
47	27.523302895151296	23.971797420428295	23.631418053049778	24.873481631370627
48	28.369064109885723	22.703649617727788	24.764994970882494	24.162291301503995
49	26.986797825524206	22.6698586906905	24.401890711052005	25.941452772733292
50	26.843927414717434	23.18413288726675	23.908464133203438	26.063475564812382
51	26.773480248116304	22.715209671714227	24.450107004602284	26.061203075567185
52	27.683760396638295	22.463062169377515	23.724096092701753	26.129081341282433
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	10.5
4	21.0
5	16.5
6	12.0
7	25.0
8	38.0
9	254.5
10	471.0
11	849.0
12	1227.0
13	1237.0
14	1227.5
15	1208.0
16	1315.5
17	1423.0
18	1432.5
19	1442.0
20	1643.5
21	1845.0
22	2095.5
23	2346.0
24	3177.0
25	4008.0
26	4997.0
27	5986.0
28	5866.5
29	5747.0
30	8023.0
31	10299.0
32	10518.0
33	10737.0
34	12337.0
35	13937.0
36	16073.5
37	18210.0
38	19818.5
39	22411.5
40	23396.0
41	28783.0
42	34170.0
43	33014.5
44	31859.0
45	35205.0
46	38551.0
47	40914.5
48	43278.0
49	46473.5
50	49669.0
51	51808.0
52	53947.0
53	59006.5
54	64066.0
55	68992.5
56	73919.0
57	87129.0
58	100339.0
59	91927.0
60	83515.0
61	79333.0
62	75151.0
63	76784.5
64	64921.0
65	51424.0
66	41171.0
67	30918.0
68	27081.5
69	23245.0
70	20735.5
71	18226.0
72	19977.5
73	21729.0
74	15722.0
75	9715.0
76	6538.5
77	3362.0
78	2053.5
79	745.0
80	675.5
81	606.0
82	373.0
83	140.0
84	103.5
85	67.0
86	41.0
87	15.0
88	10.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1012106.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.097169664047046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.19292300674465	6.294597601437004
2	10.29726412737719	1.8735191768451132
3	4.042444582016444	1.10324412660334
4	1.8702551236518847	0.6805611269965794
5	0.9731408773473221	0.44264138341240944
6	0.6473124585926385	0.3533226756881196
7	0.4463849336939168	0.28425876341015666
8	0.3160535661920433	0.23001543316609133
9	0.3030204294418559	0.2480965432474464
>10	6.138607409338243	16.088927444358596
>50	3.5667350906346056	22.822016666238518
>100	2.0635799854463306	36.9666813555102
>500	0.10969556764741022	6.363957925355644
>1k	0.029324557687921537	4.6628515195048745
>5k	0.003258284187546838	1.5853082582259168
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGCAATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5468	0.5402596170756818	No Hit
TGCAATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5395	0.533046933819185	No Hit
TGCAATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	5182	0.5120017073310503	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4590	0.45350981023726766	No Hit
TGCAATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	3221	0.3182472982078952	No Hit
TGCAATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	3062	0.3025374812519637	No Hit
TGCAATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2732	0.26993220077738894	No Hit
TGCAATGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGC	2225	0.21983863350281493	No Hit
TGCAATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	2107	0.2081797756361488	No Hit
TGCAATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2084	0.20590728639095116	No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2039	0.20146111178078185	No Hit
TGCAATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1709	0.16885583130620704	No Hit
TGCAATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1673	0.16529889161807163	No Hit
TGCAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1667	0.16470606833671572	No Hit
TGCAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1557	0.15383764151185744	No Hit
TGCAATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1530	0.15116993674575588	No Hit
TGCAATGGGGTGCCAGGCGGTGGTGGCGCACGCCTTTAATCCCAGCACTTGG	1523	0.150478309584174	No Hit
TGCAATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1456	0.14385844960903305	No Hit
TGCAATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1415	0.13980749051976768	No Hit
TGCAATGGGGGGCTGGAGAGATGGCTTAGCAGTTAAGAGCACTGACTGCTCT	1327	0.13111274905988107	No Hit
TGCAATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1246	0.12310963476157635	No Hit
TGCAATGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1181	0.11668738254688737	No Hit
TGCAATGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCATTGACTGCTC	1138	0.11243881569717007	No Hit
TGCAATGGGGGATGCAAGAGCCTAGTGCGGTGCTGTGAGAAGTATCGGTGGG	1138	0.11243881569717007	No Hit
TGCAATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1136	0.1122412079367181	No Hit
TGCAATGGGGCCGGGCAGTGGTGGCACACGCCTTTAATCCCAGCACTTGGGA	1132	0.11184599241581415	No Hit
TGCAATGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	1116	0.11026513033219841	No Hit
TGCAATGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACTGACTGCTCT	1101	0.10878307212880865	No Hit
TGCAATGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	1056	0.10433689751863935	No Hit
TGCAATGGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCT	1032	0.10196560439321573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.002865312526553543	0.0	0.0	0.0	0.0
8	0.0037545474485874007	0.0	0.0	0.0	0.0
9	0.004742586250847243	0.0	0.0	0.0	0.0
10	0.019958383805648815	0.0	0.0	0.0	0.0
11	0.03487776971977243	0.0	0.0	0.0	0.0
12	0.044362942221466924	0.0	0.0	0.0	0.0
13	0.05068639055592991	0.0	0.0	0.0	0.0
14	0.057800269932200776	0.0	0.0	0.0	0.0
15	0.0663962075118614	0.0	0.0	0.0	0.0
16	0.07657300717513778	0.0	0.0	0.0	0.0
17	0.08635459131751022	0.0	0.0	0.0	0.0
18	0.09327086293332912	0.0	0.0	0.0	0.0
19	0.10166919275253777	0.0	0.0	0.0	0.0
20	0.10868426824858266	0.0	0.0	0.0	0.0
21	0.11688499030733936	0.0	0.0	0.0	0.0
22	0.12310963476157635	0.0	0.0	0.0	0.0
23	0.13180437622146296	0.0	0.0	0.0	0.0
24	0.14010390216044563	0.0	0.0	0.0	0.0
25	0.14879864362033227	0.0	0.0	0.0	9.880388022598423E-5
26	0.15887663940338265	0.0	0.0	0.0	9.880388022598423E-5
27	0.1678677925039472	0.0	0.0	0.0	9.880388022598423E-5
28	0.1828859822982968	0.0	0.0	0.0	9.880388022598423E-5
29	0.19009866555479366	0.0	0.0	0.0	9.880388022598423E-5
30	0.19918862253558423	0.0	0.0	0.0	9.880388022598423E-5
31	0.21262595024631809	0.0	0.0	0.0	9.880388022598423E-5
32	0.225470454675696	0.0	0.0	0.0	9.880388022598423E-5
33	0.23278194181241885	0.0	0.0	0.0	9.880388022598423E-5
34	0.24147668327230548	0.0	0.0	0.0	9.880388022598423E-5
35	0.256890088587559	0.0	0.0	0.0	9.880388022598423E-5
36	0.26993220077738894	0.0	0.0	0.0	9.880388022598423E-5
37	0.2809982353626992	0.0	0.0	0.0	9.880388022598423E-5
38	0.28989058458303774	0.0	0.0	0.0	9.880388022598423E-5
39	0.29986977648586216	0.0	0.0	0.0	9.880388022598423E-5
40	0.3098489683886866	0.0	0.0	0.0	9.880388022598423E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTCG	20	6.311095E-4	46.0	19
AACGTTA	25	3.4168654E-5	46.0	39
AACCGTT	55	1.8189894E-12	46.0	32
GATACGG	40	5.6097633E-9	46.0	11
TCGTTAG	25	3.4168654E-5	46.0	20
CTCGTTA	25	3.4168654E-5	46.0	19
CTCGTAA	20	6.311095E-4	46.0	40
CTCAAAT	30	1.8611809E-6	46.0	23
CGAAATA	40	5.6097633E-9	46.0	20
ACGTTAT	20	6.311095E-4	46.0	22
TAGACGC	20	6.311095E-4	46.0	45
GACGTTA	50	1.6370905E-11	46.0	9
GGCGTAA	20	6.311095E-4	46.0	31
ACCGAAT	25	3.4168654E-5	46.0	20
GATTTCG	25	3.4168654E-5	46.0	38
CCTCGAT	25	3.4168654E-5	46.0	40
ATCGTGA	20	6.311095E-4	46.0	19
CGTTATC	20	6.311095E-4	46.0	23
CGTTAGC	20	6.311095E-4	46.0	40
GTTACGC	50	1.6370905E-11	46.0	12
>>END_MODULE