SRR1527576_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18985
Sequences flagged as poor quality	0
Sequence length	50
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGA	1657	8.727943112983935	No Hit
TTCCGCGGGAAGCTGGGTGGGAGATGATGAAATAGAGAGGAAAATTCAAG	267	1.4063734527258362	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG	237	1.2483539636555174	Illumina Single End Adapter 2 (100% over 23bp)
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	230	1.2114827495391098	No Hit
TTCCGCGGGTATCTTAACTCTGTTGCTCAGCCTCTCCCTTCTATAAAGCT	211	1.1114037397945746	No Hit
TTCCGCGGGGGCTGTACTAACTCCTGGATCTTTCCCTCTCTCCAACCCTG	178	0.9375823018172241	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCG	173	0.9112457203055044	No Hit
TTCCGCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT	158	0.832235975770345	No Hit
TTCCGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA	148	0.7795628127469054	No Hit
TTCCGCGGGGGCATCGATACAATATTGAAGAGCAGCAGAACAAAACGTAC	135	0.711087700816434	No Hit
TTCCGCGGGGCTTCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGA	131	0.6900184356070582	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGCGA	109	0.5741374769554911	No Hit
TTCCGCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCT	109	0.5741374769554911	No Hit
TTCCGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	102	0.5372662628390835	No Hit
TTCCGCGGGGTCGGTCGGCGCAGCGCCCTGCCTCGGTAGCTGCTCTCCGA	100	0.5267316302343955	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCGCGGGA	99	0.5214643139320516	No Hit
TTCCGCGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTT	99	0.5214643139320516	No Hit
TTCCGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	96	0.5056623650250197	No Hit
TTCCGCGGGCCCAGGGCTGATCTGGATCAATAAAGTGAGTGTTATCCTGC	96	0.5056623650250197	No Hit
TTCCGCGGGGCCTTTGCCCTTCAGTGCTGCCTTTGCTCCGCACCATGAAC	83	0.43718725309454837	No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	82	0.43191993679220436	No Hit
TTCCGCGGGGCCACAAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATG	82	0.43191993679220436	No Hit
TTCCGCGGGGGCCCTTCAGTGCTGCCTTTGCTCCGCACCATGAACCACAC	81	0.42665262048986036	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCC	79	0.4161179878851725	Illumina Single End Adapter 2 (95% over 22bp)
TTCCGCGGGGAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGA	79	0.4161179878851725	No Hit
TTCCGCGGGGTCTTGCAGAACTGGAGTAAACCGAGGCTACCAAACGCGCC	78	0.4108506715828285	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGAGA	77	0.4055833552804846	No Hit
TTCCGCGGGGCTCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAA	72	0.3792467737687648	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGG	67	0.352910192257045	No Hit
TTCCGCGGGGTGTTCTCATCAGCGTCTGTGTAGGGGTAGCCTCGTCTGTC	66	0.3476428759547011	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTGCGCGGGA	65	0.3423755596523571	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTC	65	0.3423755596523571	No Hit
TTCCGCGGGGTAGGTTTTTGCTGTGAGGTTAAAGACTTGCCAGGCTGAAC	64	0.33710824335001316	No Hit
TTCCGCGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAA	63	0.3318409270476692	Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCCAGCAGTCCACTTCGATTTAATTCGTAAACAAGCAGTAGTAATT	58	0.30550434553594946	No Hit
TTCCGCGGGGACAGGGGGCTCTGGACTGGTGGGTAGAGCCATCCAGAAGG	56	0.2949697129312615	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGC	56	0.2949697129312615	Illumina Single End Adapter 2 (100% over 21bp)
TTCCGCGGGAGGGCAGGACTTATGCTGACTATGAGTCTGTGAATGAGTGC	55	0.28970239662891756	No Hit
TTCCGCGGCCTTTGTTTTTCACATTTAGAATGTCTCCAATAAATTAGATA	55	0.28970239662891756	No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA	55	0.28970239662891756	Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG	53	0.27916776402422966	No Hit
TTCCGCGGGGGCGGTGCCTGGGTGCCTCGGTCGGTGGTCTCTCGTGCTTT	52	0.2739004477218857	No Hit
TTCCGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	51	0.26863313141954176	No Hit
TTCCGCGGGGGGAATTCTTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	51	0.26863313141954176	No Hit
TTCCGCGGGGGGAATTCCTTCGATTCCGTTTTGCTACCCGTTGTAGCGCC	51	0.26863313141954176	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGAA	51	0.26863313141954176	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGTGA	49	0.25809849881485386	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGCGC	48	0.25283118251250986	No Hit
TTCCGCGGGGGCCGCGGCGACAGCGAAATGGCGGAGACGGTAGCGGACAC	48	0.25283118251250986	No Hit
TTCCGCGGGCACCTGGGGAAGCACGAGAAGTTCTCGGAGGTGCTCAAGCG	48	0.25283118251250986	No Hit
TTCCGCGGGGAGCAGTAGCAGCTGCAGAGCGCGGAGGCAGAGCACAGCCT	47	0.2475638662101659	No Hit
TTCCGCGGGGAGTTCCGTGGAGACTGCGAGTTCCGTCTGCTGTGTGAGGA	46	0.24229654990782198	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGT	46	0.24229654990782198	No Hit
TTCCGCGGGCGCCACACTCATTTGATGCCCACAGTCGGGAGGCAGAGGCG	45	0.23702923360547798	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCTGGA	45	0.23702923360547798	No Hit
TTCCGCGGGAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCT	45	0.23702923360547798	No Hit
TTCCGCGGGATTTCCATCCTGCACCACTTCCTTTGTGTTTATATGGCGTT	44	0.23176191730313406	No Hit
TTCCGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCT	44	0.23176191730313406	No Hit
TTCCGCGGGGGTTTAGCTCAGGTCCCACCGATCCGACCCCTGCCGTCGCC	44	0.23176191730313406	No Hit
TTCCGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	43	0.2264946010007901	Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTGCGCGCGA	42	0.22122728469844613	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTGCGCGGGA	41	0.21595996839610218	No Hit
TTCCGCGGGCCCGGGCCAGCGGCGTGTGCTCATCAATCTGTACTGCTGCG	41	0.21595996839610218	No Hit
TTCCGCGGGGAAGTCTACCACTCAAGAACGCAGTGATCACCAGAGACACT	41	0.21595996839610218	No Hit
TTCCGCGGGCTTTCTCTTCTCCATTCCTCCGCCATCGTGCGCGCTATTCT	40	0.21069265209375823	No Hit
TTCCGCGGGAGGAGAAGAAGGAGGAGGAGGAGAAAGAAGAAGGAAGGAAG	40	0.21069265209375823	No Hit
TTCCGCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTT	40	0.21069265209375823	Illumina Single End Adapter 2 (100% over 27bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGTAGAGCTCG	39	0.20542533579141425	No Hit
TTCCGCGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT	38	0.2001580194890703	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATCCGCGGGA	38	0.2001580194890703	No Hit
TTCCGCGGGGGATGCTGAGAACCAAGCCTACATCTGTGGAAGGAGCAGCA	38	0.2001580194890703	No Hit
TTCCGCGGGGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGCCAT	38	0.2001580194890703	No Hit
TTCCGCGGGGAGGAGCCTCTCCCCTCTGCTGCTGCTGCTGCGCCAGACAC	37	0.19489070318672636	No Hit
TTCCGCGGGATTTTGAAGTAGAGCTCGTGACTCTGGTCCGCCTGCCTCCT	37	0.19489070318672636	No Hit
TTCCGCGGGGAGGGCTGTCAGTTCTTGGCCAGCCAGGACCCTTGACCGAG	36	0.1896233868843824	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGCGCAGTGCTTTCCGCGGGA	36	0.1896233868843824	No Hit
TTCCGCGGGGAGGAGGAAGCCAGCCCCGTGCCTGGAGGGGATCCGCCATC	35	0.18435607058203846	No Hit
TTCCGCGGGCAACAGTACCCATCTATTTCAGGTGGGATCTCTCTGGACCT	34	0.17908875427969448	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTA	33	0.17382143797735056	No Hit
TTCCGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	33	0.17382143797735056	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCTGTACAGCTCG	32	0.16855412167500658	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCTCCGCTGGC	32	0.16855412167500658	No Hit
TTCCGCGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGC	32	0.16855412167500658	No Hit
TTCCGCGGGATTTTACTTATTAGGTATTAATCATAAAAGGCAGTCATCAA	32	0.16855412167500658	No Hit
TTCCGCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA	32	0.16855412167500658	Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGCGCAGTGCCTTGCGCGCCG	31	0.16328680537266263	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTCGGAAGAGCTCGT	30	0.15801948907031868	No Hit
TTCCGCGGGACGTTATGTTATATTTGGGAGGACAGATGATGGGTAATGGT	30	0.15801948907031868	No Hit
TTCCGCGGGCAACCTGCAAAGGCATAACATCACTTCCTGCCCCAGCAGTC	29	0.15275217276797473	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATG	29	0.15275217276797473	No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCCCTTA	29	0.15275217276797473	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTAT	29	0.15275217276797473	No Hit
TTCCGCGGGCCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGG	29	0.15275217276797473	No Hit
TTCCGCGGGGCTTTCTCCCGCGCCTGCACGAACTGAGGTTTTGCGTGGCG	29	0.15275217276797473	No Hit
TTCCGCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGAAG	29	0.15275217276797473	No Hit
TTCCGCGGGCTCCAGTGTCTTTAGCAATTGAGCTTTCTATCGCCATAGCC	28	0.14748485646563075	No Hit
TTCCGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAG	28	0.14748485646563075	No Hit
TTCCGCGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC	27	0.1422175401632868	No Hit
TTCCGCGGAGCCACAGTGCAGCTCCATGTAAACCTACAGGCCAAACCATT	27	0.1422175401632868	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGT	27	0.1422175401632868	Illumina Single End Adapter 2 (95% over 24bp)
TTCCGCGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCC	27	0.1422175401632868	No Hit
TTCCGCGGGGAGCGGAGGAGGTAGGAACCTGATTTCCAGAAGCAGCTTGT	27	0.1422175401632868	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCCTTCCGCGGGA	26	0.13695022386094285	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCCGCG	26	0.13695022386094285	No Hit
TTCCGCGGGGATGTAGGAGCCTCTCCCCTCTGCTGCTGCTGCTGCGCCAG	25	0.13168290755859888	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGAGCGGTACAGCTCG	25	0.13168290755859888	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGAGCAGTGCTTTCCGCTGGA	25	0.13168290755859888	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCT	24	0.12641559125625493	Illumina Single End Adapter 2 (96% over 26bp)
TTCCGCGGGCCGGGGCTGGTGAAGTACATGAACTCGGGGCCCGTGGTGGC	24	0.12641559125625493	No Hit
TTCCGCGGGAACCTACAGGTGCAGCTGGAACACCTGCAGTCCAGGCTCCG	24	0.12641559125625493	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGGATCGGAAGAGC	23	0.12114827495391099	No Hit
TTCCGCGGGGACCAGGAGGATTGGCGGGAGTACCAGATCGCCGGTCCTCC	23	0.12114827495391099	No Hit
TTCCGCGGGAAGGACTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAA	23	0.12114827495391099	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTTCGCGGGA	22	0.11588095865156703	No Hit
TTCCGCGGGGCGCGAGCCCTGGACTCGAAGCAGCCATGATGGAAGGCCTG	22	0.11588095865156703	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTTCGGAAGAGCTCG	22	0.11588095865156703	No Hit
TTCCGCGGGGGGTGTGAGAAGTTTTATACTTATGTATGAATCGTTTCTAA	22	0.11588095865156703	No Hit
TTCCGCGGGGGCTCTTCAGAGACCGTGGACAACAAAATGATGGTTTCTAT	22	0.11588095865156703	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGCGCAGTGCCTTGCGCGGCG	21	0.11061364234922307	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGGATCGGAAGAGCTCGTATGCCGTC	21	0.11061364234922307	Illumina Single End Adapter 2 (96% over 25bp)
TTCCGCGGGCTCCTCCTCGCGTCACCGCCAGGATGAAACCCATCCTGCTG	21	0.11061364234922307	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCGTTGCGCTCGT	21	0.11061364234922307	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCCGGA	21	0.11061364234922307	No Hit
TTCCGCGGGGAGAAGTGTACGGCGACTTCGAGCCCCGGGCGGCCAAAGAA	20	0.10534632604687912	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCCTCG	20	0.10534632604687912	No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGTCGGA	20	0.10534632604687912	No Hit
TTCCGCGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	19	0.10007900974453515	Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGAGCAGTGCTTTTCGCATGC	19	0.10007900974453515	No Hit
TTCCGCGGGGAGCAGCAGCCATCCTCCAGACGGGGCGATTGTTCCAGAGT	19	0.10007900974453515	No Hit
TTCCGCGGGAAGGACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAA	19	0.10007900974453515	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGGGC	65	0.0	44.000004	6
CCGCGAG	20	7.6618773E-4	44.0	43
GGAGATG	35	1.3479803E-7	44.0	20
CTTTCCT	20	7.6618773E-4	44.0	20
TGATGCT	25	4.278818E-5	44.0	38
GCGGGGT	40	7.594281E-9	44.0	5
GCGGGGC	95	0.0	44.0	5
GCGGGGA	145	0.0	44.0	5
CGCTGCT	20	7.6618773E-4	44.0	29
TGATGAA	30	2.3980283E-6	44.0	25
GCGGGCT	55	1.8189894E-12	44.0	5
GCGGGCA	20	7.6618773E-4	44.0	5
TCCTTTC	20	7.6618773E-4	44.0	23
GCGGGAT	40	7.594281E-9	44.0	5
GTTGATG	25	4.278818E-5	44.0	36
CTTCAGT	20	7.6618773E-4	44.0	19
AGGGGGG	25	4.278818E-5	44.0	24
TGTTGCT	35	1.3479803E-7	44.0	21
GGAGACA	25	4.278818E-5	44.0	12
TGGTTGA	25	4.278818E-5	44.0	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content