FastQCFastQC Report
Fri 17 Jun 2016
SRR1527575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15099
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG157610.437777336247434No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG2571.7020994767865423No Hit
TTCCGCGGGAAGCTGGGTGGGAGATGATGAAATAGAGAGGAAAATTCAAGAT2451.6226240148354196No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC1931.2782303463805549Illumina Single End Adapter 2 (100% over 25bp)
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC1761.1656401086164647No Hit
TTCCGCGGGTATCTTAACTCTGTTGCTCAGCCTCTCCCTTCTATAAAGCTCT1721.1391482879660904No Hit
TTCCGCGGGGGCTGTACTAACTCCTGGATCTTTCCCTCTCTCCAACCCTGTT1561.0331810053645938No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA1310.867607126299755No Hit
TTCCGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC1300.8609841711371614No Hit
TTCCGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1220.808000529836413No Hit
TTCCGCGGGGCTTCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACG1220.808000529836413No Hit
TTCCGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC1170.7748857540234453No Hit
TTCCGCGGGGGCATCGATACAATATTGAAGAGCAGCAGAACAAAACGTACAA1130.748393933373071No Hit
TTCCGCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA1120.7417709782104774No Hit
TTCCGCGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTT1020.675541426584542No Hit
TTCCGCGGGGCCACAAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAG810.5364593681700774No Hit
TTCCGCGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA800.529836413007484Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGTCGGTCGGCGCAGCGCCCTGCCTCGGTAGCTGCTCTCCGACC790.5232134578448904No Hit
TTCCGCGGGGCCTTTGCCCTTCAGTGCTGCCTTTGCTCCGCACCATGAACCA790.5232134578448904No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCGG780.5165905026822969No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCAG770.5099675475197033No Hit
TTCCGCGGGCCCAGGGCTGATCTGGATCAATAAAGTGAGTGTTATCCTGCGT770.5099675475197033No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT750.49672163719451623Illumina Single End Adapter 2 (95% over 24bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGTAG750.49672163719451623No Hit
TTCCGCGGGGTGTTCTCATCAGCGTCTGTGTAGGGGTAGCCTCGTCTGTCTC740.49009868203192264No Hit
TTCCGCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTT730.4834757268693291No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG690.45698390621895485No Hit
TTCCGCGGGGCTCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAG630.4172461752433936No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA630.4172461752433936Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGGCCCTTCAGTGCTGCCTTTGCTCCGCACCATGAACCACACTT620.4106232200808001No Hit
TTCCGCGGGGGCGGTGCCTGGGTGCCTCGGTCGGTGGTCTCTCGTGCTTTCG600.397377309755613No Hit
TTCCGCGGGGTCTTGCAGAACTGGAGTAAACCGAGGCTACCAAACGCGCCGC600.397377309755613No Hit
TTCCGCCAGCAGTCCACTTCGATTTAATTCGTAAACAAGCAGTAGTAATTCC580.38413139943042585No Hit
TTCCGCGGGCGCCACACTCATTTGATGCCCACAGTCGGGAGGCAGAGGCGGT560.3708854891052387No Hit
TTCCGCGGGAGGGCAGGACTTATGCTGACTATGAGTCTGTGAATGAGTGCAT550.36426253394264524No Hit
TTCCGCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG550.36426253394264524No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG540.35763957878005165Illumina Single End Adapter 2 (100% over 23bp)
TTCCGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA510.337770713292271No Hit
TTCCGCGGGGACAGGGGGCTCTGGACTGGTGGGTAGAGCCATCCAGAAGGTG490.3245248029670839No Hit
TTCCGCGGGGAGCAGTAGCAGCTGCAGAGCGCGGAGGCAGAGCACAGCCTCA480.3179018478044904No Hit
TTCCGCGGGGGGAATTCTTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG460.30465593747930325No Hit
TTCCGCGGGGTAGGTTTTTGCTGTGAGGTTAAAGACTTGCCAGGCTGAACTT460.30465593747930325No Hit
TTCCGCGGGGAGGGCTGTCAGTTCTTGGCCAGCCAGGACCCTTGACCGAGTG450.2980329823167097No Hit
TTCCGCGGGGGTTTAGCTCAGGTCCCACCGATCCGACCCCTGCCGTCGCCGC450.2980329823167097No Hit
TTCCGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA450.2980329823167097Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGCTTTCTCTTCTCCATTCCTCCGCCATCGTGCGCGCTATTCTTG420.27816411682892905No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCTTTCCGCGGGGAG420.27816411682892905No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT410.2715411616663355Illumina Single End Adapter 2 (96% over 26bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGGGCAGTGCTTTCCGCGGGAAG410.2715411616663355No Hit
TTCCGCGGGGGCCGCGGCGACAGCGAAATGGCGGAGACGGTAGCGGACACCC410.2715411616663355No Hit
TTCCGCGGGGGGAATTCCTTCGATTCCGTTTTGCTACCCGTTGTAGCGCCGG410.2715411616663355No Hit
TTCCGCGGCCTTTGTTTTTCACATTTAGAATGTCTCCAATAAATTAGATAGA410.2715411616663355No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT400.264918206503742No Hit
TTCCGCGGGCCCGGGCCAGCGGCGTGTGCTCATCAATCTGTACTGCTGCGCT390.25829525134114845No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCTTTCCGCGGGCGG380.25167229617855486No Hit
TTCCGCGGGGAGCGGAGGAGGTAGGAACCTGATTTCCAGAAGCAGCTTGTAG380.25167229617855486No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGGGGGGAG380.25167229617855486No Hit
TTCCGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT370.24504934101596132No Hit
TTCCGCGGGGAAGTCTACCACTCAAGAACGCAGTGATCACCAGAGACACTTG370.24504934101596132No Hit
TTCCGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG370.24504934101596132No Hit
TTCCGCGGGATTTTGAAGTAGAGCTCGTGACTCTGGTCCGCCTGCCTCCTGG360.23842638585336778No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGAAAG350.23180343069077425No Hit
TTCCGCGGGGAGGAGCCTCTCCCCTCTGCTGCTGCTGCTGCGCCAGACACAG350.23180343069077425No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCCG340.22518047552818068No Hit
TTCCGCGGGAGGAGAAGAAGGAGGAGGAGGAGAAAGAAGAAGGAAGGAAGGA340.22518047552818068No Hit
TTCCGCGGGATTTCCATCCTGCACCACTTCCTTTGTGTTTATATGGCGTTTC340.22518047552818068No Hit
TTCCGCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA330.21855752036558712Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGATTCTCGCTGGGGGTCTCACCGGGCGCTGTCTCCTTGACAGAT330.21855752036558712No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCCCTTAAT320.21193456520299356No Hit
TTCCGCGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTC310.20531161004040005No Hit
TTCCGCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT310.20531161004040005Illumina Single End Adapter 2 (100% over 29bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTTCGCGGGAAG310.20531161004040005No Hit
TTCCGCGGGCCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCG310.20531161004040005No Hit
TTCCGCGGGCCGGGGCTGGTGAAGTACATGAACTCGGGGCCCGTGGTGGCCA310.20531161004040005No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTCGGAAGAGCTCGTAT310.20531161004040005No Hit
TTCCGCGGGGCTTTCTCCCGCGCCTGCACGAACTGAGGTTTTGCGTGGCGGC310.20531161004040005No Hit
TTCCGCGGGGAGGAGGAAGCCAGCCCCGTGCCTGGAGGGGATCCGCCATCTC300.1986886548778065No Hit
TTCCGCGGGACGTTATGTTATATTTGGGAGGACAGATGATGGGTAATGGTGT300.1986886548778065No Hit
TTCCGCGGGCAACCTGCAAAGGCATAACATCACTTCCTGCCCCAGCAGTCCG300.1986886548778065No Hit
TTCCGCGGGGATGTAGGAGCCTCTCCCCTCTGCTGCTGCTGCTGCGCCAGAC280.18544274455261936No Hit
TTCCGCGGGCACCTGGGGAAGCACGAGAAGTTCTCGGAGGTGCTCAAGCGGC280.18544274455261936No Hit
TTCCGCGGGGAGAAGTGTACGGCGACTTCGAGCCCCGGGCGGCCAAAGAAAG270.17881978939002582No Hit
TTCCGCGGGGAGTTCCGTGGAGACTGCGAGTTCCGTCTGCTGTGTGAGGACT270.17881978939002582No Hit
TTCCGCGGGCAACAGTACCCATCTATTTCAGGTGGGATCTCTCTGGACCTTT270.17881978939002582No Hit
TTCCGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA270.17881978939002582Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTAT260.1721968342274323No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG260.1721968342274323No Hit
TTCCGCGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA250.16557387906483872No Hit
TTCCGCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGAAGCA250.16557387906483872No Hit
TTCCGCGGGATTTTACTTATTAGGTATTAATCATAAAAGGCAGTCATCAATT240.1589509239022452No Hit
TTCCGCGGGAAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTC240.1589509239022452Illumina Single End Adapter 2 (96% over 28bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGTAGAGCTCGTA240.1589509239022452No Hit
TTCCGCGGGGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGCCATCA240.1589509239022452No Hit
TTCCGCGGGGCTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACGCCCCC230.15232796873965163No Hit
TTCCGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAA230.15232796873965163No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAA230.15232796873965163No Hit
TTCCGCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA220.1457050135770581Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGAACCTACAGGTGCAGCTGGAACACCTGCAGTCCAGGCTCCGCA220.1457050135770581No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGC220.1457050135770581Illumina Single End Adapter 2 (95% over 21bp)
TTCCGCGGGGCGCGAGCCCTGGACTCGAAGCAGCCATGATGGAAGGCCTGGA220.1457050135770581No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGAGATCGGAAGAGCTCGTA210.13908205841446453No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCTTTCCGCGGGAAG200.132459103251871No Hit
TTCCGCGGGAAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.132459103251871No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCC200.132459103251871Illumina Single End Adapter 2 (95% over 22bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCCGGAAG200.132459103251871No Hit
TTCCGCGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG190.12583614808927743No Hit
TTCCGCGGGAAGCAGTGGGATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG190.12583614808927743No Hit
TTCCGCGGGCTCCTCCTCGCGTCACCGCCAGGATGAAACCCATCCTGCTGCA190.12583614808927743No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCCGGGAG180.11921319292668389No Hit
TTCCGCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA180.11921319292668389Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGGATGCTGAGAACCAAGCCTACATCTGTGGAAGGAGCAGCACA180.11921319292668389No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTTCGGAAGAGCTCGTA180.11921319292668389No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGGATCGGAAGAGCTC170.11259023776409034No Hit
TTCCGCGGGGTACAGCAATATAGAGGAAAAAAAAAAAAAAAAAAAAAAAAAA170.11259023776409034No Hit
TTCCGCGGGCTCCAGTGTCTTTAGCAATTGAGCTTTCTATCGCCATAGCCCC170.11259023776409034No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT170.11259023776409034No Hit
TTCCGCGGGAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTA170.11259023776409034No Hit
TTCCGCGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.11259023776409034No Hit
TTCCGCGGGGAGCAGCAGCCATCCTCCAGACGGGGCGATTGTTCCAGAGTCA160.10596728260149678No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTATCGGAAGAGCTCGT160.10596728260149678No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCCGTGCTTTCCGCGGGAAG160.10596728260149678No Hit
TTCCGCGGGGGGTGTGAGAAGTTTTATACTTATGTATGAATCGTTTCTAAAT160.10596728260149678No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCACT550.046.00000415
GGAGATG405.00404E-946.020
GCGGGGC1000.046.05
GATGGCT301.7411785E-646.020
GCGGGCT206.1140454E-446.05
GCGGGCG206.1140454E-446.045
GCGGGAT253.2580734E-546.05
AGGGGCT253.2580734E-546.035
GGTGAGA253.2580734E-546.015
TCCTTCG206.1140454E-446.016
GTGCAGG501.4551915E-1146.031
CTATAAA206.1140454E-446.041
ATCTTAA206.1140454E-446.011
CTCCCTT206.1140454E-446.034
GAAATAG405.00404E-946.029
AAATTCA405.00404E-946.042
AGGACTG301.7411785E-646.011
CTGGGTG405.00404E-946.013
CGGGGTA206.1140454E-446.06
GGGTATC206.1140454E-446.07
Produced by FastQC (version 0.11.5)