FastQCFastQC Report
Fri 17 Jun 2016
SRR1527574_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527574_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20479
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG212810.391132379510719No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGGAG4312.104594950925338No Hit
TTCCGCGGGAAGCTGGGTGGGAGATGATGAAATAGAGAGGAAAATTCAAGAT3201.5625762976707849No Hit
TTCCGCGGGTATCTTAACTCTGTTGCTCAGCCTCTCCCTTCTATAAAGCTCT2501.2207627325553005No Hit
TTCCGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC2451.1963474779041945No Hit
TTCCGCGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA2081.01567459348601No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC1970.9619610332535768Illumina Single End Adapter 2 (100% over 25bp)
TTCCGCGGGGGCTGTACTAACTCCTGGATCTTTCCCTCTCTCCAACCCTGTT1730.844767810928268No Hit
TTCCGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1700.8301186581376043No Hit
TTCCGCGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTTT1680.8203525562771619No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA1620.7910542506958348No Hit
TTCCGCGGGGGCATCGATACAATATTGAAGAGCAGCAGAACAAAACGTACAA1590.7764050979051712No Hit
TTCCGCGGGGCTTCTTTCCCCGTTCTCTTCGGTTCTCATCGCTGTGCGGACG1530.7471067923238439No Hit
TTCCGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC1410.6885101811611896No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGTAG1390.6787440793007471No Hit
TTCCGCGGGGTCGGTCGGCGCAGCGCCCTGCCTCGGTAGCTGCTCTCCGACC1370.6689779774403046No Hit
TTCCGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC1350.6592118755798623No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCAG1270.6201474681380926No Hit
TTCCGCGGGGCCACAAGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAG1250.6103813662776503No Hit
TTCCGCGGGCCCAGGGCTGATCTGGATCAATAAAGTGAGTGTTATCCTGCGT1210.5908491625567656No Hit
TTCCGCGGGGCCTTTGCCCTTCAGTGCTGCCTTTGCTCCGCACCATGAACCA1040.507837296743005No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCGG930.4541237365105718No Hit
TTCCGCGGGGGCCCTTCAGTGCTGCCTTTGCTCCGCACCATGAACCACACTT820.40041017627813863No Hit
TTCCGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG790.385761023487475No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGT790.385761023487475Illumina Single End Adapter 2 (95% over 24bp)
TTCCGCGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA730.35646271790614775Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGTAGGTTTTTGCTGTGAGGTTAAAGACTTGCCAGGCTGAACTT730.35646271790614775No Hit
TTCCGCGGGGCTCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAG720.3515796669759266No Hit
TTCCGCGGGCGCCACACTCATTTGATGCCCACAGTCGGGAGGCAGAGGCGGT720.3515796669759266No Hit
TTCCGCGGGGTCTTGCAGAACTGGAGTAAACCGAGGCTACCAAACGCGCCGC720.3515796669759266No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGATCGGAAGAGCTCGTATGCCG710.34669661604570534Illumina Single End Adapter 2 (100% over 23bp)
TTCCGCGGGGTGTTCTCATCAGCGTCTGTGTAGGGGTAGCCTCGTCTGTCTC700.34181356511548416No Hit
TTCCGCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG660.32228136139459934No Hit
TTCCGCGGGGGGAATTCCTTCGATTCCGTTTTGCTACCCGTTGTAGCGCCGG630.30763220860393575No Hit
TTCCGCGGGAGGGCAGGACTTATGCTGACTATGAGTCTGTGAATGAGTGCAT620.3027491576737145No Hit
TTCCGCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA600.29298305581327216Illumina Single End Adapter 2 (100% over 34bp)
TTCCGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT580.28321695395282975No Hit
TTCCGCGGGGGCCGCGGCGACAGCGAAATGGCGGAGACGGTAGCGGACACCC580.28321695395282975No Hit
TTCCGCGGGGGCGGTGCCTGGGTGCCTCGGTCGGTGGTCTCTCGTGCTTTCG560.2734508520923873No Hit
TTCCGCGGGGAGCAGTAGCAGCTGCAGAGCGCGGAGGCAGAGCACAGCCTCA550.2685678011621661No Hit
TTCCGCGGGGAGCGGAGGAGGTAGGAACCTGATTTCCAGAAGCAGCTTGTAG550.2685678011621661No Hit
TTCCGCGGGCCTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCG540.26368475023194493No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGATCGGAAGAGCTCGT540.26368475023194493No Hit
TTCCGCGGGGACAGGGGGCTCTGGACTGGTGGGTAGAGCCATCCAGAAGGTG540.26368475023194493No Hit
TTCCGCGGGCCCGGGCCAGCGGCGTGTGCTCATCAATCTGTACTGCTGCGCT530.25880169930172375No Hit
TTCCGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG530.25880169930172375No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTTCGCGGGAAG510.24903559744128131No Hit
TTCCGCGGGGGGAATTCTTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG510.24903559744128131No Hit
TTCCGCGGGGCTTTCTCCCGCGCCTGCACGAACTGAGGTTTTGCGTGGCGGC510.24903559744128131No Hit
TTCCGCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGGTGCTTT510.24903559744128131No Hit
TTCCGCGGGGGTTTAGCTCAGGTCCCACCGATCCGACCCCTGCCGTCGCCGC500.2441525465110601No Hit
TTCCGCGGGCACCTGGGGAAGCACGAGAAGTTCTCGGAGGTGCTCAAGCGGC500.2441525465110601No Hit
TTCCGCGGGATTTCCATCCTGCACCACTTCCTTTGTGTTTATATGGCGTTTC490.2392694955808389No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGAAAG480.23438644465061773No Hit
TTCCGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCCCTTAAT480.23438644465061773No Hit
TTCCGCGGGCAACAGTACCCATCTATTTCAGGTGGGATCTCTCTGGACCTTT480.23438644465061773No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTAT480.23438644465061773No Hit
TTCCGCGGCCTTTGTTTTTCACATTTAGAATGTCTCCAATAAATTAGATAGA460.22462034279017531No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGTAGAGCTCGTA460.22462034279017531No Hit
TTCCGCGGGGCTCTTTCCCGTCTTGCAAGATGGCGGGTGAAAAAGCGCCTGA450.2197372918599541No Hit
TTCCGCGGGAAGCAGTGGGATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAG450.2197372918599541No Hit
TTCCGCGGGGAGTTCCGTGGAGACTGCGAGTTCCGTCTGCTGTGTGAGGACT430.20997118999951167No Hit
TTCCGCGGGCCGGGGCTGGTGAAGTACATGAACTCGGGGCCCGTGGTGGCCA430.20997118999951167No Hit
TTCCGCGGGGGGCTGGTGAGATAGCTCAGTGGGTAAGAGCACCCGACTGCTC410.20020508813906931No Hit
TTCCGCGGGAGGAGAAGAAGGAGGAGGAGGAGAAAGAAGAAGGAAGGAAGGA400.1953220372088481No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTCGGAAGAGCTCGTAT400.1953220372088481No Hit
TTCCGCGGGGAGGAGCCTCTCCCCTCTGCTGCTGCTGCTGCGCCAGACACAG400.1953220372088481No Hit
TTCCGCGGGCTTTCTCTTCTCCATTCCTCCGCCATCGTGCGCGCTATTCTTG390.19043898627862688No Hit
TTCCGCGGGAAGCAGTGGTATCAAGATCGGAAGAGCTCGTATGCCGTCTTCT390.19043898627862688Illumina Single End Adapter 2 (100% over 29bp)
TTCCGCGGGACGTTATGTTATATTTGGGAGGACAGATGATGGGTAATGGTGT390.19043898627862688No Hit
TTCCGCCAGCAGTCCACTTCGATTTAATTCGTAAACAAGCAGTAGTAATTCC390.19043898627862688No Hit
TTCCGCGGGGAGGGCTGTCAGTTCTTGGCCAGCCAGGACCCTTGACCGAGTG380.18555593534840567No Hit
TTCCGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA380.18555593534840567Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCCGGGAG370.18067288441818447No Hit
TTCCGCGGGGAGGAGGAAGCCAGCCCCGTGCCTGGAGGGGATCCGCCATCTC360.1757898334879633No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGAAA360.1757898334879633No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGATCGGAAGAGCTCGTATG360.1757898334879633No Hit
TTCCGCGGGATTTTACTTATTAGGTATTAATCATAAAAGGCAGTCATCAATT350.17090678255774208No Hit
TTCCGCGGGGAAGTCTACCACTCAAGAACGCAGTGATCACCAGAGACACTTG350.17090678255774208No Hit
TTCCGCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGAAGCA350.17090678255774208No Hit
TTCCGCGGGATTCTCGCTGGGGGTCTCACCGGGCGCTGTCTCCTTGACAGAT350.17090678255774208No Hit
TTCCGCGGGGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGCCATCA340.16602373162752088No Hit
TTCCGCGGGAACCTACAGGTGCAGCTGGAACACCTGCAGTCCAGGCTCCGCA330.16114068069729967No Hit
TTCCGCGGGCAACCTGCAAAGGCATAACATCACTTCCTGCCCCAGCAGTCCG320.15625762976707847No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCCGGAAG320.15625762976707847No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGGATCGGAAGAGCTC320.15625762976707847No Hit
TTCCGCGGGATTTTGAAGTAGAGCTCGTGACTCTGGTCCGCCTGCCTCCTGG320.15625762976707847No Hit
TTCCGCGGGAAGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA300.14649152790663608Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGCGTAG300.14649152790663608No Hit
TTCCGCGGGGAGAAGTGTACGGCGACTTCGAGCCCCGGGCGGCCAAAGAAAG300.14649152790663608No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTT300.14649152790663608No Hit
TTCCGCGGGGGATGCTGAGAACCAAGCCTACATCTGTGGAAGGAGCAGCACA290.14160847697641488No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCTGGCGG280.13672542604619364No Hit
TTCCGCGGGGGCTCTTCAGAGACCGTGGACAACAAAATGATGGTTTCTATCT280.13672542604619364No Hit
TTCCGCGGGAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTA280.13672542604619364No Hit
TTCCGCGGGCTCCTCCTCGCGTCACCGCCAGGATGAAACCCATCCTGCTGCA280.13672542604619364No Hit
TTCCGCGGGAGGTGTGACCTTTGACCCCCTGTGTCCAGACCTGGTCTCCAGA280.13672542604619364No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGC270.13184237511597247Illumina Single End Adapter 2 (95% over 21bp)
TTCCGCGGGGCGCGAGCCCTGGACTCGAAGCAGCCATGATGGAAGGCCTGGA270.13184237511597247No Hit
TTCCGCGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA270.13184237511597247No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGGATCGGAAGAGCTCGTATGCC260.12695932418575126Illumina Single End Adapter 2 (95% over 22bp)
TTCCGCGGGGAGCAGCAGCCATCCTCCAGACGGGGCGATTGTTCCAGAGTCA260.12695932418575126No Hit
TTCCGCGGAGCCACAGTGCAGCTCCATGTAAACCTACAGGCCAAACCATTTG260.12695932418575126No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGTGG260.12695932418575126No Hit
TTCCGCGGGAAGGACTGCACTCTGCCCAAAAAAAAAAAAAAAAAAAAAAAAA250.12207627325553005No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT250.12207627325553005Illumina Single End Adapter 2 (96% over 26bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTCCGCGGGCCG250.12207627325553005No Hit
TTCCGCGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAA240.11719322232530886No Hit
TTCCGCGGGGATGTAGGAGCCTCTCCCCTCTGCTGCTGCTGCTGCGCCAGAC240.11719322232530886No Hit
TTCCGCGGGAAGCAGTGGTATCAACGATCGGAAGAGCTCGTATGCCGTCTTC240.11719322232530886Illumina Single End Adapter 2 (96% over 28bp)
TTCCGCGGGGGGCTGGTGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCT240.11719322232530886No Hit
TTCCGCGGGAAGCAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGA230.11231017139508766Illumina Single End Adapter 2 (97% over 34bp)
TTCCGCGGGGACCAGGAGGATTGGCGGGAGTACCAGATCGCCGGTCCTCCCA230.11231017139508766No Hit
TTCCGCGGGAAGGACTGCACTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAA220.10742712046486645No Hit
TTCCGCGGGAAGCAGTGGTATCAACGGATCGGAAGAGCTCGTATGCCGTCTT220.10742712046486645Illumina Single End Adapter 2 (96% over 27bp)
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGGGCTTTCCGCGGGCGG210.10254406953464523No Hit
TTCCGCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTTCGGAAGAGCTCGTA210.10254406953464523No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAA206.166448E-446.025
CGCTGCC253.3000913E-546.022
GAGGTGG206.166448E-446.032
GTTGATG206.166448E-446.036
TAGCGCC253.3000913E-546.044
TGTTGCT301.772738E-646.021
GGAGACA301.772738E-646.012
TGGTTGA206.166448E-446.034
ACAAAAC301.772738E-646.040
AACGTAC301.772738E-646.044
GGTTGAT206.166448E-446.035
TTGCAAG253.3000913E-546.023
GGGCATC301.772738E-646.09
GTGTCCG206.166448E-446.034
CCTACGA206.166448E-446.027
GGCATCG301.772738E-646.010
GTGCAGG405.160473E-946.031
TCGATTC253.3000913E-546.020
CGATACA301.772738E-646.015
CTATAAA301.772738E-646.041
Produced by FastQC (version 0.11.5)