FastQCFastQC Report
Fri 17 Jun 2016
SRR1527573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103109
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA326101.5505615733659073No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA139540.6634939035494594No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC96890.46069889863055125No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA90390.429792274199768No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAA79400.3775363045852592No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG71620.3405434525742603No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGA65720.31248974732170326No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC52570.24996326866558033No Hit
TATTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG45910.21829586578727017No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT45370.21572823852686668No Hit
TATTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC44180.2100699488233848No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43280.20579057005604562No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT42180.20056021822929768No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAA40240.19133577955303316No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAA40220.19124068224709226No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA39290.18681865752084176No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC35500.16879771804504665No Hit
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA32320.15367724640044808No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA30870.14678269171973493No Hit
TATTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA29430.1399356856919922No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAA29090.1383190314909974No Hit
TATTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA28580.13589405018950518No Hit
TATTGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA28320.13465778521227384No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAA26820.12752548726670848No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAGTGCAGTGCTTATTGTGG25080.11925202164985267No Hit
TATTGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC24920.1184912432023257No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG23830.11330844002854822No Hit
TATTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT23130.10998003432061772No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAA22510.10703201783645071No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA21060.10013746315573754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.857973E-444.00000437
TAAGTCG207.857973E-444.00000444
CCGTCTA207.857973E-444.00000441
GCCGATA207.857973E-444.00000412
TATTCGC408.3182385E-944.00000425
TATTCGA408.3182385E-944.00000414
ACTACGG207.857973E-444.00000443
TCGTCAA207.857973E-444.00000436
CCAACGA207.857973E-444.00000420
GACCGTT351.4468606E-744.044
TAACGGG254.4441033E-544.031
AATGCGC502.7284841E-1144.029
GTCGTTA302.5287227E-644.039
GCGATAG351.4468606E-744.013
ATAACGG254.4441033E-544.034
CGATAGC302.5287227E-644.014
GTTATCG351.4468606E-744.05
TACGTAA551.8189894E-1244.040
CGTATCT650.040.61538741
TAGACCG603.6379788E-1240.33333242
Produced by FastQC (version 0.11.5)