##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527573_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2103109
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.120406502943975	31.0	31.0	33.0	30.0	33.0
2	29.50051043478964	31.0	28.0	31.0	25.0	33.0
3	31.191932039661282	31.0	31.0	33.0	28.0	34.0
4	35.26250422588653	35.0	35.0	37.0	35.0	37.0
5	35.34644186297524	35.0	35.0	37.0	33.0	37.0
6	34.900692260838596	35.0	35.0	37.0	33.0	37.0
7	36.07613205021708	37.0	35.0	37.0	35.0	37.0
8	36.54083502091427	37.0	37.0	37.0	35.0	37.0
9	38.51448450841112	39.0	39.0	39.0	37.0	39.0
10	36.8287554282731	39.0	37.0	39.0	32.0	39.0
11	36.84223737333633	38.0	37.0	39.0	33.0	39.0
12	36.97539594952045	39.0	37.0	39.0	34.0	39.0
13	36.96954033290714	39.0	37.0	39.0	33.0	39.0
14	37.886459998031484	40.0	38.0	40.0	34.0	40.0
15	37.97974855321336	40.0	38.0	40.0	34.0	40.0
16	38.0718840535607	40.0	38.0	40.0	34.0	40.0
17	38.04855288052117	40.0	38.0	40.0	34.0	40.0
18	37.87516481551836	40.0	38.0	40.0	34.0	40.0
19	37.81546510428133	40.0	37.0	40.0	34.0	40.0
20	37.67004230403655	39.0	37.0	40.0	33.0	40.0
21	37.61577930578016	39.0	37.0	40.0	33.0	40.0
22	37.44525319419964	39.0	36.0	40.0	33.0	40.0
23	37.17665323100229	38.0	36.0	40.0	33.0	40.0
24	37.12596969534152	38.0	36.0	40.0	33.0	40.0
25	36.731548864086456	38.0	36.0	40.0	32.0	40.0
26	36.03755820549482	38.0	35.0	40.0	31.0	40.0
27	35.793018336187046	38.0	34.0	39.0	30.0	40.0
28	35.77593172774212	38.0	34.0	39.0	30.0	40.0
29	35.43384246845979	37.0	34.0	39.0	30.0	40.0
30	35.12011693164739	37.0	34.0	38.0	30.0	40.0
31	35.09012324135363	37.0	34.0	38.0	30.0	40.0
32	34.97238516881436	37.0	34.0	38.0	29.0	40.0
33	34.532853504026654	36.0	33.0	38.0	28.0	40.0
34	34.108415683637894	36.0	33.0	38.0	26.0	40.0
35	33.957398308884606	36.0	33.0	38.0	26.0	40.0
36	33.630116175623805	35.0	32.0	38.0	25.0	40.0
37	33.25517269908502	35.0	32.0	38.0	24.0	40.0
38	32.74638214186711	35.0	31.0	38.0	23.0	39.0
39	31.865003668378577	34.0	30.0	37.0	20.0	39.0
40	31.589842466557844	34.0	30.0	37.0	19.0	39.0
41	31.19854605728947	34.0	29.0	37.0	19.0	38.0
42	30.805804168970795	33.0	29.0	36.0	18.0	38.0
43	30.337928752147416	33.0	28.0	35.0	18.0	38.0
44	29.885880855438305	32.0	27.0	35.0	18.0	38.0
45	29.402485082798847	32.0	27.0	35.0	17.0	37.0
46	29.21118971960084	32.0	26.0	35.0	17.0	37.0
47	28.87025874550487	31.0	26.0	35.0	15.0	37.0
48	28.284669030468702	31.0	24.0	34.0	14.0	36.0
49	27.428331104093985	30.0	24.0	33.0	12.0	35.0
50	29.845943315348848	33.0	27.0	35.0	15.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	15.0
13	35.0
14	36.0
15	91.0
16	176.0
17	384.0
18	778.0
19	1461.0
20	2502.0
21	4002.0
22	6264.0
23	9441.0
24	13337.0
25	18828.0
26	26776.0
27	36717.0
28	49752.0
29	60704.0
30	66915.0
31	77224.0
32	106466.0
33	159332.0
34	230088.0
35	433210.0
36	597275.0
37	198344.0
38	2947.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.54985690232888	0.27159790576712856	0.14602191327220795	0.032523278631778
8	98.78632063292963	0.895911719268949	0.1664678340494953	0.1512998137519263
9	95.87605777922114	2.3926006688193526	0.8040001730770968	0.927341378882407
10	49.840260300345825	31.407834781744548	7.410885503319134	11.341019414590495
11	27.637702087718708	33.83295872919568	21.020451151129112	17.5088880319565
12	34.65759501766195	21.51871348560631	24.710321718940865	19.113369777790883
13	22.38405142101527	22.462839538987282	30.90224995470991	24.250859085287544
14	26.87235896950657	24.263839867548473	26.834082303865376	22.02971885907958
15	24.316809066957536	23.552797311028577	26.74521387146363	25.385179750550257
16	26.62325157659446	28.720147172590675	25.789390849451934	18.867210401362936
17	32.6574133818076	23.046023767669674	23.19485105146714	21.10171179905559
18	27.536803846115443	24.544899955256717	27.61754145885924	20.3007547397686
19	29.545116301627733	23.60781110251537	24.824818875293673	22.022253720563224
20	23.32832012035515	24.782833414720777	26.751490293655728	25.137356171268344
21	24.73195635604241	24.175494470329404	31.752942905003973	19.339606268624213
22	22.695637743930533	24.33378393606798	31.251352164818847	21.719226155182636
23	23.69049821003096	29.868684885091547	27.993366011937564	18.44745089293993
24	25.72662662753096	24.341249074584344	31.38054185493952	18.551582442945183
25	26.96469845357516	23.301455131426856	28.610404881534908	21.123441533463076
26	31.67001805422353	22.79924625875311	25.306106340660424	20.22462934636293
27	27.62624286235283	22.141886131436838	28.616015622585422	21.61585538362491
28	33.46849830417729	25.870699046031376	20.99933954921024	19.66146310058109
29	34.09033958772465	25.31499793876589	21.466980551174476	19.127681922334983
30	30.944615804506565	27.692525684593615	21.594363392482272	19.768495118417544
31	29.29334618414928	30.680007550726092	20.70767611188959	19.318970153235043
32	28.51411885926978	32.19676203183002	21.23499067333172	18.054128435568483
33	28.45011837237157	32.49061270718731	20.72417549447033	18.33509342597079
34	25.290890771709883	35.004652635693155	21.17075244316866	18.5337041494283
35	24.850067210020974	36.744552945187344	21.543248590539054	16.86213125425263
36	25.814448989567353	36.725390838040255	20.05207528473322	17.408084887659175
37	25.29293536378761	37.38917003350753	19.952175564842335	17.36571903786252
38	25.874027451739305	35.65093392686732	19.46779743703251	19.00724118436087
39	24.65307314076446	35.05438852670023	19.648767610237986	20.643770722297322
40	24.41532987591228	35.00446244108127	20.217164207846576	20.36304347515987
41	22.256097995871826	35.494546407247554	21.207127162691044	21.042228434189575
42	21.758216050618394	35.724729436277435	21.708337513652406	20.808716999451764
43	22.81327311137939	34.05539132779138	22.107413358033273	21.023922202795955
44	23.165418435278436	33.97403558255896	21.82321505922898	21.03733092293362
45	24.393124654975086	33.87156823540767	20.92292886388675	20.812378245730486
46	23.24648888859303	35.17668366214019	20.875522856875225	20.70130459239155
47	23.987677291096183	34.78497785896975	20.642772200584943	20.58457264934913
48	23.893198117643927	35.29793272721481	20.590373585011523	20.218495570129747
49	24.014304536759624	35.008599173889706	19.969483274523576	21.007613014827093
50	22.217678684271714	36.06132635065515	20.886126206487635	20.834868758585504
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	12.5
2	25.0
3	60.5
4	96.0
5	225.0
6	354.0
7	3319.5
8	6285.0
9	5211.0
10	4137.0
11	4105.5
12	4074.0
13	4247.0
14	4420.0
15	5614.5
16	6809.0
17	8083.5
18	9358.0
19	11554.0
20	13750.0
21	17350.0
22	20950.0
23	26721.0
24	32492.0
25	38404.0
26	44316.0
27	62352.0
28	80388.0
29	94333.0
30	108278.0
31	97287.5
32	86297.0
33	75912.0
34	65527.0
35	66628.0
36	67729.0
37	67285.5
38	66842.0
39	67444.0
40	68046.0
41	72864.5
42	77683.0
43	82477.5
44	87272.0
45	92728.0
46	98184.0
47	117368.5
48	136553.0
49	135374.0
50	134195.0
51	136029.5
52	137864.0
53	136431.5
54	134999.0
55	136547.0
56	138095.0
57	129647.0
58	121199.0
59	119878.5
60	118558.0
61	99715.5
62	80873.0
63	66690.5
64	52508.0
65	43057.0
66	33606.0
67	29331.0
68	25056.0
69	20157.5
70	15259.0
71	14341.5
72	13424.0
73	8819.5
74	4215.0
75	2821.5
76	1428.0
77	1502.5
78	1577.0
79	952.5
80	328.0
81	182.0
82	36.0
83	23.5
84	11.0
85	7.5
86	4.0
87	6.5
88	9.0
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2103109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.014228384343323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.03183181272479	13.10637179326023
2	8.436767885511427	2.870901912595694
3	3.112342770739463	1.588623321351633
4	1.5301362103898586	1.0413634777090661
5	0.9060648111924103	0.7707996814322289
6	0.6502942984594547	0.6638553426615289
7	0.538300935613472	0.641114254062331
8	0.4095735626937994	0.5574862508689169
9	0.3505847378792218	0.5368435918507997
>10	4.899385343735761	20.48796708846029
>50	1.2658451926352197	14.962396429270871
>100	0.8054819395181632	27.059093295646118
>500	0.040569919300552953	4.534998847286852
>1k	0.02056669520097476	6.757463216261744
>5k	0.0016904133041897062	2.188670102299728
>10k+	5.634711013965687E-4	2.2320513949820304
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA	32610	1.5505615733659073	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA	13954	0.6634939035494594	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9689	0.46069889863055125	No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA	9039	0.429792274199768	No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAA	7940	0.3775363045852592	No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	7162	0.3405434525742603	No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGA	6572	0.31248974732170326	No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	5257	0.24996326866558033	No Hit
TATTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	4591	0.21829586578727017	No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	4537	0.21572823852686668	No Hit
TATTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	4418	0.2100699488233848	No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4328	0.20579057005604562	No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4218	0.20056021822929768	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAA	4024	0.19133577955303316	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAA	4022	0.19124068224709226	No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA	3929	0.18681865752084176	No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3550	0.16879771804504665	No Hit
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA	3232	0.15367724640044808	No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	3087	0.14678269171973493	No Hit
TATTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	2943	0.1399356856919922	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAA	2909	0.1383190314909974	No Hit
TATTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	2858	0.13589405018950518	No Hit
TATTGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2832	0.13465778521227384	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAA	2682	0.12752548726670848	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAGTGCAGTGCTTATTGTGG	2508	0.11925202164985267	No Hit
TATTGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	2492	0.1184912432023257	No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	2383	0.11330844002854822	No Hit
TATTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2313	0.10998003432061772	No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAA	2251	0.10703201783645071	No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA	2106	0.10013746315573754	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.705838356452281E-4	0.0	0.0	0.0	0.0
8	0.008368562922796679	0.0	0.0	0.0	0.0
9	0.018211134087676863	0.0	0.0	0.0	0.0
10	0.1402209776098148	0.0	0.0	0.0	0.0
11	0.28766935047113584	0.0	0.0	0.0	0.0
12	0.3445850880767473	0.0	0.0	0.0	0.0
13	0.3859524161610264	0.0	0.0	0.0	0.0
14	0.4282707173047141	0.0	0.0	0.0	0.0
15	0.47510614048059324	0.0	0.0	0.0	0.0
16	0.537394875871864	0.0	0.0	0.0	0.0
17	0.5748156657595969	0.0	0.0	0.0	0.0
18	0.612046261035448	0.0	0.0	0.0	0.0
19	0.6473749101924817	0.0	0.0	0.0	0.0
20	0.6895981140302285	0.0	0.0	0.0	0.0
21	0.7356252101056103	0.0	0.0	0.0	0.0
22	0.7892125420032913	0.0	0.0	0.0	0.0
23	0.8546394884906108	0.0	0.0	0.0	0.0
24	0.919923789019019	0.0	0.0	0.0	0.0
25	0.979739994455827	0.0	0.0	0.0	0.0
26	1.041838535235216	0.0	0.0	0.0	0.0
27	1.1131615146908695	0.0	0.0	0.0	0.0
28	1.2076882367960957	0.0	0.0	0.0	0.0
29	1.2666961151324063	0.0	0.0	0.0	0.0
30	1.341109757031138	0.0	0.0	0.0	0.0
31	1.4121949932219395	0.0	0.0	0.0	0.0
32	1.4819964157825392	0.0	0.0	0.0	0.0
33	1.5507993166307594	0.0	0.0	0.0	0.0
34	1.6229306231869105	0.0	0.0	0.0	0.0
35	1.7169343101094618	0.0	0.0	0.0	0.0
36	1.7867357326700612	0.0	0.0	0.0	0.0
37	1.8628611260757288	0.0	0.0	0.0	0.0
38	1.9369419274036677	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.857973E-4	44.000004	37
TAAGTCG	20	7.857973E-4	44.000004	44
CCGTCTA	20	7.857973E-4	44.000004	41
GCCGATA	20	7.857973E-4	44.000004	12
TATTCGC	40	8.3182385E-9	44.000004	25
TATTCGA	40	8.3182385E-9	44.000004	14
ACTACGG	20	7.857973E-4	44.000004	43
TCGTCAA	20	7.857973E-4	44.000004	36
CCAACGA	20	7.857973E-4	44.000004	20
GACCGTT	35	1.4468606E-7	44.0	44
TAACGGG	25	4.4441033E-5	44.0	31
AATGCGC	50	2.7284841E-11	44.0	29
GTCGTTA	30	2.5287227E-6	44.0	39
GCGATAG	35	1.4468606E-7	44.0	13
ATAACGG	25	4.4441033E-5	44.0	34
CGATAGC	30	2.5287227E-6	44.0	14
GTTATCG	35	1.4468606E-7	44.0	5
TACGTAA	55	1.8189894E-12	44.0	40
CGTATCT	65	0.0	40.615387	41
TAGACCG	60	3.6379788E-12	40.333332	42
>>END_MODULE