FastQCFastQC Report
Fri 17 Jun 2016
SRR1527572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1686442
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA266241.5787083101583097No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA113580.6734889192750181No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC74390.4411061868715319No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA72690.4310257927637002No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA63180.37463488219577074No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT55780.3307555196087384No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA47020.27881184173544066No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG37950.22502997434836183No Hit
TATTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG37900.2247334921687197No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC36370.21566113747167112No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35850.21257772280339318No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG35050.2078340079291194No Hit
TATTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA34890.20688526495426465No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAAAA34330.20356466454227304No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAA33620.19935461759135503No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA31770.188384776944597No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC28970.17178177488463878No Hit
TATTGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG25920.15369636192647004No Hit
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA23410.13881295650843611No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAAAA23310.1382199921491519No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA22960.1361446168916571No Hit
TATTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC22240.1318752735048107No Hit
TATTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA22210.13169738419702545No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAAAA21030.12470040475747164No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC20020.11871146472870102No Hit
TATTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT18620.11040996369872191No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA18140.10756373477415766No Hit
TATTGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG18090.10726725259451554No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA16920.10032956959089016No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGAAAAAAAAAAAAAAAAAAAAAAA16880.10009238384717647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG351.01994374E-746.00000437
TACGCGA351.01994374E-746.00000411
ACGTAAC351.01994374E-746.00000411
CGGCGTA206.312297E-446.010
GATACGG206.312297E-446.045
CGAACAA206.312297E-446.036
CACGGAT206.312297E-446.020
ACACCGT253.4178396E-546.019
TCGATAG253.4178396E-546.043
TCGATAA301.8619248E-646.019
AATATCG206.312297E-446.042
CGGATTA301.8619248E-646.015
CGCTATC206.312297E-446.022
CTACGCG206.312297E-446.040
GTCGTTA253.4178396E-546.039
CGTAAAC453.110472E-1046.040
ATACGGG453.110472E-1046.022
ATACGGA206.312297E-446.046
TATACGG453.110472E-1046.021
TGTACGG206.312297E-446.045
Produced by FastQC (version 0.11.5)