##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527572_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1686442
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50137389842046	31.0	31.0	33.0	30.0	33.0
2	30.520386114672192	31.0	30.0	33.0	28.0	33.0
3	31.84151663680103	33.0	31.0	34.0	30.0	34.0
4	35.836625273801296	37.0	35.0	37.0	35.0	37.0
5	35.836407062917075	37.0	35.0	37.0	35.0	37.0
6	35.07338764096245	35.0	35.0	37.0	33.0	37.0
7	36.19497676172676	37.0	35.0	37.0	35.0	37.0
8	36.62833705517296	37.0	37.0	37.0	35.0	37.0
9	38.61187458566615	39.0	39.0	39.0	39.0	39.0
10	37.18592160299613	39.0	37.0	39.0	34.0	39.0
11	37.2805557499161	39.0	37.0	39.0	34.0	39.0
12	37.5021364505865	39.0	37.0	39.0	35.0	39.0
13	37.31815561993831	39.0	37.0	39.0	34.0	39.0
14	38.24864774477865	40.0	38.0	40.0	35.0	40.0
15	38.37940765232365	40.0	38.0	40.0	35.0	40.0
16	38.42563515377345	40.0	38.0	40.0	35.0	40.0
17	38.47797018812387	40.0	38.0	40.0	35.0	40.0
18	38.311522127650996	40.0	38.0	40.0	35.0	40.0
19	38.295909376071045	40.0	38.0	40.0	35.0	40.0
20	38.29203969066236	40.0	38.0	40.0	34.0	40.0
21	38.24699337421625	40.0	38.0	40.0	34.0	40.0
22	38.254244142401575	40.0	38.0	40.0	34.0	40.0
23	37.96425136470747	40.0	38.0	40.0	34.0	40.0
24	37.987941476789594	39.0	38.0	40.0	34.0	40.0
25	37.8663114414845	39.0	37.0	40.0	34.0	40.0
26	37.63894933831107	39.0	37.0	40.0	34.0	40.0
27	37.50208664158032	39.0	36.0	40.0	34.0	40.0
28	37.48702890464066	39.0	36.0	40.0	34.0	40.0
29	37.440210217724655	39.0	36.0	40.0	33.0	40.0
30	37.27999777045401	39.0	36.0	40.0	33.0	40.0
31	37.119249283402574	39.0	36.0	40.0	33.0	40.0
32	37.02047328043301	39.0	35.0	40.0	33.0	40.0
33	36.80113754282685	39.0	35.0	40.0	32.0	40.0
34	36.47448237176256	39.0	35.0	40.0	31.0	40.0
35	36.37685909150745	39.0	35.0	40.0	31.0	40.0
36	36.18360133345825	39.0	35.0	40.0	30.0	40.0
37	35.92406083339955	39.0	35.0	40.0	30.0	40.0
38	35.95764811360248	39.0	35.0	40.0	30.0	40.0
39	35.719798249806395	39.0	35.0	40.0	28.0	40.0
40	35.47439342710867	39.0	35.0	40.0	27.0	40.0
41	35.24606538499397	38.0	35.0	40.0	26.0	40.0
42	34.94747165926845	38.0	35.0	40.0	26.0	40.0
43	34.81656351063363	38.0	35.0	40.0	26.0	40.0
44	34.6391556899081	37.0	35.0	40.0	25.0	40.0
45	34.441334478149855	37.0	34.0	40.0	24.0	40.0
46	34.251353441150066	37.0	34.0	40.0	23.0	40.0
47	34.077498662865366	36.0	34.0	40.0	23.0	40.0
48	33.85440708900751	36.0	34.0	40.0	22.0	40.0
49	33.66696571835853	35.0	33.0	40.0	21.0	40.0
50	33.78037904653703	36.0	34.0	40.0	20.0	40.0
51	33.73334570652296	36.0	34.0	40.0	20.0	40.0
52	33.25152540081426	35.0	33.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	8.0
12	8.0
13	19.0
14	27.0
15	50.0
16	106.0
17	217.0
18	452.0
19	783.0
20	1370.0
21	2338.0
22	3848.0
23	6219.0
24	8967.0
25	12492.0
26	18308.0
27	24942.0
28	28895.0
29	27532.0
30	24921.0
31	29095.0
32	38195.0
33	53147.0
34	96295.0
35	112941.0
36	202292.0
37	400419.0
38	519301.0
39	73254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.53962247145172	0.2784560631198701	0.14841897912884047	0.03350248629955848
8	98.8058290768375	0.887015385053266	0.1630059023672323	0.14414963574199408
9	95.86016002922129	2.387274510478273	0.8100486112181742	0.9425168490822691
10	50.07127431598596	31.16525798100379	7.455518778588294	11.307948924421948
11	27.662973289327468	33.747321283506935	21.01691015759807	17.572795269567525
12	34.34271679666422	21.806205016241297	24.75744792883479	19.093630258259697
13	22.401363343654868	22.669798309102834	31.084852013884856	23.84398633335745
14	27.02642604963586	24.072514797425587	26.837507604767907	22.063551548170647
15	24.025314834426563	23.751009521821683	26.732493616738672	25.491182027013082
16	26.62321028532259	28.96939236570247	25.418543893000766	18.988853455974176
17	32.421393679711485	23.242661176607317	23.25956066084692	21.076384482834275
18	27.230998753588914	24.77849816358938	27.650105962731004	20.3403971200907
19	29.66974257045306	23.832779306966977	24.446497418826144	22.05098070375382
20	23.41426506218417	24.68599572354104	26.72887653414704	25.170862680127748
21	24.855346344552615	24.364490447937136	31.439029625685315	19.34113358182493
22	22.88943230778171	24.546708395545178	31.29428702558404	21.269572271089075
23	23.838175282636463	29.714214897399376	27.987087608112226	18.460522211851934
24	25.880937500370603	24.173081552760188	31.30146189433138	18.644519052537827
25	27.04575668774853	23.480321291808433	28.662770495516597	20.811151524926444
26	31.515403435161126	22.9663990816168	25.29562238132115	20.222575101900926
27	27.688055681725192	22.39887289334587	28.58378764285994	21.329283782068995
28	33.51618377625795	25.67322208531334	21.01341166787829	19.797182470550425
29	33.906650806846606	25.41978911815526	21.499938924670996	19.17362115032714
30	31.080404781190225	27.43148000346291	21.63460113066444	19.853514084682423
31	28.987774260840276	30.817128605668024	20.76157970449028	19.433517429001412
32	28.603474059588173	32.26455460668081	21.093995524305015	18.037975809425998
33	28.241350725373298	32.54508604505818	20.863747463594954	18.34981576597357
34	25.453588086634465	35.045379562416024	21.18211002809465	18.318922322854863
35	25.10089288573221	36.37794836703545	21.676760896609547	16.84439785062279
36	25.94764599079008	36.628238623089324	20.011064714944244	17.413050671176357
37	25.888112369117945	37.14927640559236	19.72335840781954	17.239252817470152
38	26.063036855106787	35.58485853649281	19.420590805969017	18.93151380243139
39	24.96119048268485	35.08243983487129	19.689618735776268	20.2667509466676
40	24.925494028255937	34.683671303252645	20.085896817085914	20.3049378514055
41	22.778785158339275	35.27918540928179	21.0378417994808	20.904187632898136
42	22.56774914287002	34.93876457061672	21.838462277386355	20.655024009126908
43	23.364159573824654	33.551761637815	22.176748444358	20.907330344002343
44	23.813626558162095	33.58312945242113	21.62647751894225	20.976766470474526
45	24.767113247891125	33.416269281718556	21.013530260750144	20.80308720964018
46	23.71276332064785	34.9228731257879	20.648738586918494	20.715624966645755
47	24.197926759414198	34.47583729532352	20.676370726061137	20.649865219201136
48	24.187905661742295	34.9180701144777	20.63160191693518	20.262422306844822
49	24.25004832659528	34.505544809723666	20.284302691702415	20.96010417197864
50	22.255138332655378	35.84238295772994	20.94794840261331	20.954530307001367
51	22.486394432776223	35.337473805799426	20.604444149279963	21.571687612144387
52	22.769831396514082	34.99023387700259	21.446809318079126	20.793125408404205
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	8.0
2	16.0
3	47.0
4	78.0
5	176.5
6	275.0
7	2723.0
8	5171.0
9	4171.5
10	3172.0
11	3213.0
12	3254.0
13	3068.0
14	3519.0
15	4156.0
16	4593.0
17	5030.0
18	5839.0
19	6648.0
20	8259.5
21	9871.0
22	14251.5
23	18632.0
24	24265.5
25	29899.0
26	45887.5
27	61876.0
28	74143.5
29	86411.0
30	76547.5
31	66684.0
32	60374.0
33	54064.0
34	54770.5
35	55477.0
36	54327.0
37	53177.0
38	52457.5
39	53685.5
40	55633.0
41	58224.0
42	60815.0
43	63817.0
44	66819.0
45	71074.0
46	75329.0
47	83201.0
48	91073.0
49	97473.5
50	103874.0
51	105315.0
52	106756.0
53	105780.0
54	104804.0
55	107504.5
56	110205.0
57	103239.0
58	96273.0
59	92429.0
60	88585.0
61	78686.0
62	68787.0
63	59597.0
64	39356.5
65	28306.0
66	24988.5
67	21671.0
68	19229.5
69	16788.0
70	14117.0
71	11446.0
72	8892.0
73	6338.0
74	3945.0
75	1552.0
76	1625.0
77	1698.0
78	1137.5
79	577.0
80	350.0
81	123.0
82	78.5
83	34.0
84	30.5
85	27.0
86	14.5
87	2.0
88	5.5
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1686442.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.060677073369945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.13696561520497	11.906898639453384
2	8.874590377582125	2.8506386042556553
3	3.2175097357523734	1.550261545390282
4	1.7337082200933513	1.113781114494652
5	1.0507179808744052	0.8437621093003559
6	0.8054700019696868	0.7761836156353074
7	0.6465063623801699	0.7268330938446897
8	0.5328731671657024	0.6846643087129781
9	0.4606868041473736	0.6659047794036415
>10	6.316147093156843	24.250856783816005
>50	1.3181528210477187	14.526151219531632
>100	0.8475167859599594	26.01876988587349
>500	0.03757646856099876	4.106393355839785
>1k	0.01934630064526669	6.119705023428012
>5k	0.0014881769727128225	1.5896641421178013
>10k+	7.440884863564113E-4	2.2695317789023575
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA	26624	1.5787083101583097	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	11358	0.6734889192750181	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7439	0.4411061868715319	No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	7269	0.4310257927637002	No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA	6318	0.37463488219577074	No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5578	0.3307555196087384	No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4702	0.27881184173544066	No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG	3795	0.22502997434836183	No Hit
TATTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3790	0.2247334921687197	No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3637	0.21566113747167112	No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3585	0.21257772280339318	No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3505	0.2078340079291194	No Hit
TATTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	3489	0.20688526495426465	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAAAA	3433	0.20356466454227304	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAA	3362	0.19935461759135503	No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA	3177	0.188384776944597	No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2897	0.17178177488463878	No Hit
TATTGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2592	0.15369636192647004	No Hit
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	2341	0.13881295650843611	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAAAA	2331	0.1382199921491519	No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA	2296	0.1361446168916571	No Hit
TATTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	2224	0.1318752735048107	No Hit
TATTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2221	0.13169738419702545	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAAAA	2103	0.12470040475747164	No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2002	0.11871146472870102	No Hit
TATTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1862	0.11040996369872191	No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA	1814	0.10756373477415766	No Hit
TATTGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	1809	0.10726725259451554	No Hit
TATTGTGGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAA	1692	0.10032956959089016	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGAAAAAAAAAAAAAAAAAAAAAAA	1688	0.10009238384717647	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0013045215904252858	0.0	0.0	0.0	0.0
8	0.008775872517406469	0.0	0.0	0.0	0.0
9	0.019389934548594022	0.0	0.0	0.0	0.0
10	0.1500792793348363	0.0	0.0	0.0	0.0
11	0.3071555381092264	0.0	0.0	0.0	0.0
12	0.37012835306521064	0.0	0.0	0.0	0.0
13	0.4160237944738094	0.0	0.0	0.0	0.0
14	0.46352023965247546	0.0	0.0	0.0	0.0
15	0.5180729607066238	0.0	0.0	0.0	0.0
16	0.5843070796386712	0.0	0.0	0.0	0.0
17	0.6253995097370677	0.0	0.0	0.0	0.0
18	0.6643572681420411	0.0	0.0	0.0	0.0
19	0.7047381410092965	0.0	0.0	0.0	0.0
20	0.749625543007112	0.0	0.0	0.0	0.0
21	0.7929712376707886	0.0	0.0	0.0	0.0
22	0.84627873357044	0.0	0.0	0.0	0.0
23	0.9016023082916578	0.0	0.0	0.0	0.0
24	0.9539610612164545	0.0	0.0	0.0	0.0
25	0.9945198233914953	0.0	0.0	0.0	0.0
26	1.037331850131816	0.0	0.0	0.0	0.0
27	1.0860142240290505	0.0	0.0	0.0	0.0
28	1.1590674330928665	0.0	0.0	0.0	0.0
29	1.2079869927338147	0.0	0.0	0.0	0.0
30	1.2714341791772263	0.0	0.0	0.0	0.0
31	1.3358301085954927	0.0	0.0	0.0	0.0
32	1.4007597059371149	0.0	0.0	0.0	0.0
33	1.4598189561218233	0.0	0.0	0.0	0.0
34	1.5248671463353023	0.0	0.0	0.0	5.9296435928422086E-5
35	1.6056288920698132	0.0	0.0	0.0	5.9296435928422086E-5
36	1.6691353749491533	0.0	0.0	0.0	5.9296435928422086E-5
37	1.735962458240485	0.0	0.0	0.0	5.9296435928422086E-5
38	1.8094307423558	0.0	0.0	0.0	5.9296435928422086E-5
39	1.8898367094747404	0.0	0.0	0.0	5.9296435928422086E-5
40	1.977180359597306	0.0	0.0	0.0	5.9296435928422086E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	35	1.01994374E-7	46.000004	37
TACGCGA	35	1.01994374E-7	46.000004	11
ACGTAAC	35	1.01994374E-7	46.000004	11
CGGCGTA	20	6.312297E-4	46.0	10
GATACGG	20	6.312297E-4	46.0	45
CGAACAA	20	6.312297E-4	46.0	36
CACGGAT	20	6.312297E-4	46.0	20
ACACCGT	25	3.4178396E-5	46.0	19
TCGATAG	25	3.4178396E-5	46.0	43
TCGATAA	30	1.8619248E-6	46.0	19
AATATCG	20	6.312297E-4	46.0	42
CGGATTA	30	1.8619248E-6	46.0	15
CGCTATC	20	6.312297E-4	46.0	22
CTACGCG	20	6.312297E-4	46.0	40
GTCGTTA	25	3.4178396E-5	46.0	39
CGTAAAC	45	3.110472E-10	46.0	40
ATACGGG	45	3.110472E-10	46.0	22
ATACGGA	20	6.312297E-4	46.0	46
TATACGG	45	3.110472E-10	46.0	21
TGTACGG	20	6.312297E-4	46.0	45
>>END_MODULE