##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527571_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2289253
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.681341031332053	31.0	31.0	33.0	28.0	33.0
2	29.160354491181184	31.0	28.0	31.0	25.0	33.0
3	30.6955098453513	31.0	30.0	33.0	28.0	34.0
4	35.15448445409922	35.0	35.0	37.0	33.0	37.0
5	35.213579276733505	35.0	35.0	37.0	33.0	37.0
6	34.76109936297998	35.0	35.0	37.0	33.0	37.0
7	36.03946571217773	37.0	35.0	37.0	35.0	37.0
8	36.46215468539301	37.0	37.0	37.0	35.0	37.0
9	38.493248234249336	39.0	39.0	39.0	37.0	39.0
10	36.538619366229945	39.0	35.0	39.0	32.0	39.0
11	36.53179399568331	38.0	35.0	39.0	32.0	39.0
12	36.768080242769145	39.0	35.0	39.0	33.0	39.0
13	36.536863771719425	39.0	35.0	39.0	32.0	39.0
14	37.478366960750954	39.0	37.0	40.0	33.0	40.0
15	37.60819227931557	39.0	37.0	40.0	33.0	40.0
16	37.62318887427471	39.0	37.0	40.0	33.0	40.0
17	37.7392599245256	39.0	37.0	40.0	33.0	40.0
18	37.57700612383166	39.0	37.0	40.0	33.0	40.0
19	37.52547883523577	39.0	37.0	40.0	33.0	40.0
20	37.49957256799489	39.0	37.0	40.0	33.0	40.0
21	37.54445926247558	39.0	37.0	40.0	33.0	40.0
22	37.5534211378122	39.0	37.0	40.0	33.0	40.0
23	37.47585347709493	39.0	37.0	40.0	33.0	40.0
24	37.493059526404465	39.0	37.0	40.0	33.0	40.0
25	37.39396737713132	39.0	37.0	40.0	33.0	40.0
26	37.143659962441895	39.0	36.0	40.0	33.0	40.0
27	37.00610242729834	39.0	35.0	40.0	32.0	40.0
28	36.86342575503887	39.0	35.0	40.0	32.0	40.0
29	36.81492565478783	39.0	35.0	40.0	32.0	40.0
30	36.6504361903206	39.0	35.0	40.0	31.0	40.0
31	36.399541247734525	38.0	35.0	40.0	31.0	40.0
32	36.31839862173381	38.0	35.0	40.0	31.0	40.0
33	36.19776057954276	38.0	35.0	40.0	30.0	40.0
34	35.94959054329076	38.0	35.0	40.0	30.0	40.0
35	35.8369485592025	38.0	35.0	40.0	30.0	40.0
36	35.69576101898742	38.0	35.0	40.0	29.0	40.0
37	35.48646043054219	38.0	35.0	40.0	28.0	40.0
38	35.311445261838685	38.0	35.0	40.0	27.0	40.0
39	35.02049882647309	38.0	34.0	40.0	26.0	40.0
40	35.07808136540609	38.0	35.0	40.0	26.0	40.0
41	35.03286093760716	38.0	35.0	40.0	26.0	40.0
42	34.924933155051015	38.0	35.0	40.0	26.0	40.0
43	34.79830625972752	38.0	34.0	40.0	26.0	40.0
44	34.62217456960851	37.0	34.0	40.0	25.0	40.0
45	34.45696653013013	37.0	34.0	40.0	24.0	40.0
46	34.18081749810965	37.0	34.0	40.0	23.0	40.0
47	34.08707338157906	36.0	34.0	40.0	23.0	40.0
48	33.91997083764879	36.0	33.0	40.0	23.0	40.0
49	33.753845031545225	36.0	33.0	40.0	22.0	40.0
50	33.489413795679205	35.0	33.0	40.0	20.0	40.0
51	33.24723785444422	35.0	33.0	39.0	20.0	40.0
52	32.616716020466065	35.0	33.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	6.0
13	11.0
14	28.0
15	72.0
16	194.0
17	458.0
18	953.0
19	1797.0
20	2975.0
21	4482.0
22	6752.0
23	10036.0
24	14093.0
25	19710.0
26	28127.0
27	37707.0
28	44355.0
29	45596.0
30	45797.0
31	55408.0
32	74064.0
33	101993.0
34	159486.0
35	185775.0
36	310693.0
37	499419.0
38	594660.0
39	44602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.57274272437341	0.26615668954021243	0.12956191386447893	0.03153867222189946
8	98.84128141363144	0.8662214268147733	0.1512283701277229	0.14126878942607043
9	95.92430369207771	2.3610321794926117	0.7772404360723781	0.9374236923572886
10	50.06497752760398	31.289507974872155	7.2818513287958995	11.363663168727966
11	27.54457458393633	33.63573183042678	21.028125768536725	17.791567817100162
12	34.13261880622194	21.639285828171896	24.897335506385705	19.330759859220453
13	22.196934982721437	22.560328631217256	30.87033193797278	24.372404448088524
14	26.487286464187225	24.20462045916288	27.092593086041607	22.21549999060829
15	24.18893848779493	23.47495012565234	26.98181459192147	25.35429679463126
16	26.449239118612056	28.585525496745007	25.864550576104957	19.10068480853798
17	32.32106717780866	23.18634069716191	23.198134937466502	21.29445718756293
18	27.448036542924704	24.585661785744083	27.488573783675285	20.47772788765593
19	29.338478534264233	23.72695372682705	24.677831589605866	22.25673614930285
20	23.456254070650996	24.86116650278497	26.58779960100522	25.094779825558817
21	24.895500846782774	24.154647826168624	31.362457535274608	19.587393791773998
22	22.876851095095212	24.32527116924167	30.95380894990637	21.84406878575675
23	23.845988189160394	29.659718694264026	27.789283229070794	18.70500988750479
24	25.783214000374794	24.41101966449318	30.92563382028985	18.880132514842177
25	27.00819874430655	23.351329014311656	28.247904447433292	21.392567793948505
26	31.404086835312654	22.8714344810294	25.225521163453756	20.49895752020419
27	27.477478461314675	22.334228676341148	28.30425470666632	21.884038155677857
28	33.09385201198819	25.931821428212608	21.05057850748694	19.92374805231226
29	33.72069404299132	25.31327904779419	21.54003948012736	19.42598742908713
30	30.775934333164574	27.473940189223296	21.694019839659486	20.056105637952644
31	29.26552897386178	30.320065104206485	20.784378135575228	19.63002778635651
32	28.653189490196144	31.67557277417568	21.476787406197566	18.194450329430605
33	28.60898293023969	31.89822182170341	20.914027414182705	18.578767833874192
34	25.389897927402522	34.51807205232449	21.306600886839504	18.78542913343348
35	25.056514068126152	36.12178295714803	21.800386414258274	17.021316560467543
36	26.1096960449544	36.14681295601665	20.20545566610593	17.53803533292301
37	25.78391291831877	36.703632145507726	20.06676413659827	17.445690799575235
38	26.169453529164315	35.02129297198693	19.605303564088373	19.203949934760377
39	24.828666818390104	34.5894053649815	19.78025146193977	20.801676354688624
40	24.705919354479384	34.27790637382587	20.525996908161744	20.490177363532997
41	22.587149607317322	34.8131901541682	21.424455925142393	21.17520431337209
42	22.162644321095133	34.82341182909884	21.983240821350893	21.030703028455132
43	23.179700976694146	33.12255133006269	22.504502560442205	21.19324513280096
44	23.5706582016055	33.193360454261715	22.033038724859157	21.202942619273625
45	24.768123051493216	32.9445456662064	21.18704223604818	21.100289046252204
46	23.726910044455547	34.39589245924326	20.985317044468218	20.89188045183298
47	24.359780242725463	34.04612771065496	20.759522866192594	20.83456918042698
48	24.34392354187152	34.44743765761146	20.79210991533046	20.416528885186565
49	24.378694709584305	34.150878037508306	20.318505643543986	21.151921609363402
50	22.23585597572658	35.55124750300644	21.011788561596294	21.201107959670686
51	22.548097567197683	35.058248258274645	20.67115342865118	21.722500745876495
52	22.909918650319558	34.6210750843179	21.383219766447834	21.08578649891471
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	9.5
2	19.0
3	59.0
4	99.0
5	224.0
6	349.0
7	3556.5
8	6764.0
9	5554.5
10	4345.0
11	4385.0
12	4425.0
13	4314.5
14	5147.5
15	6091.0
16	6866.5
17	7642.0
18	8913.0
19	10184.0
20	12532.0
21	14880.0
22	21023.5
23	27167.0
24	34290.0
25	41413.0
26	60816.5
27	80220.0
28	95812.5
29	111405.0
30	98797.0
31	86189.0
32	78090.5
33	69992.0
34	70818.5
35	71645.0
36	70734.0
37	69823.0
38	69507.5
39	71409.0
40	73626.0
41	77153.5
42	80681.0
43	86564.5
44	92448.0
45	98353.0
46	104258.0
47	116225.5
48	128193.0
49	135875.5
50	143558.0
51	144869.5
52	146181.0
53	144683.5
54	143186.0
55	148375.5
56	153565.0
57	142432.0
58	131299.0
59	126216.5
60	121134.0
61	108144.5
62	95155.0
63	82088.5
64	53924.0
65	38826.0
66	34303.5
67	29781.0
68	26432.5
69	23084.0
70	19236.5
71	15389.0
72	11895.0
73	8401.0
74	5233.0
75	2065.0
76	2201.0
77	2337.0
78	1597.0
79	857.0
80	484.0
81	111.0
82	68.5
83	26.0
84	21.0
85	16.0
86	9.5
87	3.0
88	2.5
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2289253.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.34942215795349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.08432958071488	12.602842460779298
2	8.496313818313489	2.7781964280412197
3	3.178262238597073	1.5588825320251753
4	1.5687923925510217	1.0259539641601019
5	0.944865754250856	0.7724004549420191
6	0.6230903451430314	0.6112300257173018
7	0.5028628922064731	0.5755062398577162
8	0.38970208404777573	0.5097123110345086
9	0.3515372588497671	0.5172687944266152
>10	4.528678076261692	18.112720553129773
>50	1.3839403558209413	15.853000569579356
>100	0.874730956868199	28.50381301964447
>500	0.04726110479248179	5.0392162275903996
>1k	0.022963022667533525	6.883548499845641
>5k	0.0018690832403806357	2.1176119547092696
>10k+	8.010356744488439E-4	2.5380959645171295
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATTGTGGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAA	33810	1.4769009803634636	No Hit
TATTGTGGGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	14075	0.6148293788410455	No Hit
TATTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	10255	0.4479627197168683	No Hit
TATTGTGGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	8950	0.39095722491135754	No Hit
TATTGTGGGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAA	8400	0.3669319205871959	No Hit
TATTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7455	0.32565207952113634	No Hit
TATTGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATTGTGGGAAG	7329	0.3201481007123284	No Hit
TATTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6102	0.2665498308836987	No Hit
TATTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	5229	0.22841512056552943	No Hit
TATTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5043	0.22029019946681297	No Hit
TATTGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4994	0.2181497632633877	No Hit
TATTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	4820	0.21054903062265287	No Hit
TATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4704	0.2054818755288297	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGGAAAAAAAAAAAAAAAAAAAAA	4412	0.1927266230512748	No Hit
TATTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4274	0.18669845578448516	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGTAAAAAAAAAAAAAAAAAAAAA	4172	0.18224285389164063	No Hit
TATTGTGGGAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAA	3993	0.17442370939341348	No Hit
TATTGTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	3324	0.14520020286093324	No Hit
TATTGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	3129	0.13668214041873047	No Hit
TATTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	3109	0.13580849298876096	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGGGGGAAAAAAAAAAAAAAAAAAAAAA	3037	0.13266336224087072	No Hit
TATTGTGGGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	2991	0.13065397315194083	No Hit
TATTGTGGGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAA	2881	0.1258489122871085	No Hit
TATTGTGGGGAGTGCAGTGCTTATTGTGGGCAAAAAAAAAAAAAAAAAAAAA	2709	0.11833554438937068	No Hit
TATTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2655	0.11597669632845298	No Hit
TATTGTGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	2600	0.11357416589603683	No Hit
TATTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2520	0.11007957617615877	No Hit
TATTGTGGGACGCAGAGTGCAGTGCTTATTGTGGGAAAAAAAAAAAAAAAAA	2407	0.10514346819683101	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.115532009786599E-4	0.0	0.0	0.0	0.0
8	0.008518062442202762	0.0	0.0	0.0	0.0
9	0.016686665912417718	0.0	0.0	0.0	0.0
10	0.12501894722863746	0.0	0.0	0.0	0.0
11	0.2565902501820463	0.0	0.0	0.0	0.0
12	0.310101155267679	0.0	0.0	0.0	0.0
13	0.35033261941777516	0.0	0.0	0.0	0.0
14	0.39065144831086823	0.0	0.0	0.0	0.0
15	0.43586270281179057	0.0	0.0	0.0	0.0
16	0.49356711556127697	0.0	0.0	0.0	0.0
17	0.5300418957625042	0.0	0.0	0.0	0.0
18	0.5648567458467894	0.0	0.0	0.0	0.0
19	0.6008510199615333	0.0	0.0	0.0	0.0
20	0.6414319430836173	0.0	0.0	0.0	0.0
21	0.6819255014627043	0.0	0.0	0.0	0.0
22	0.7278793562791006	0.0	0.0	0.0	0.0
23	0.7781140835023477	0.0	0.0	0.0	0.0
24	0.8291787757840658	0.0	0.0	0.0	0.0
25	0.8738658418270064	0.0	0.0	0.0	0.0
26	0.9200381085008953	0.0	0.0	0.0	0.0
27	0.9708407065536225	0.0	0.0	0.0	0.0
28	1.047372221418952	0.0	0.0	0.0	0.0
29	1.0943744531513118	0.0	0.0	0.0	0.0
30	1.1571460209946214	0.0	0.0	0.0	0.0
31	1.217427693662518	0.0	0.0	0.0	0.0
32	1.2763552128139617	0.0	0.0	0.0	0.0
33	1.3339285784489525	0.0	0.0	0.0	0.0
34	1.394778121946329	0.0	0.0	0.0	0.0
35	1.4754594621040138	0.0	0.0	0.0	0.0
36	1.5365274174588828	0.0	0.0	0.0	0.0
37	1.6013957391341194	0.0	0.0	0.0	0.0
38	1.6706322979592032	0.0	0.0	0.0	0.0
39	1.7432760817611683	0.0	0.0	0.0	0.0
40	1.8210307030284552	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	30	1.8622195E-6	46.0	37
ATAATCG	20	6.3127733E-4	46.0	45
TAGCGGA	130	0.0	46.0	11
CGTACTC	20	6.3127733E-4	46.0	22
GCGATAG	65	0.0	46.0	13
TTACGCC	20	6.3127733E-4	46.0	15
ATACGGG	20	6.3127733E-4	46.0	22
TATCGAT	50	1.6370905E-11	46.0	11
CGATAGC	30	1.8622195E-6	46.0	14
AATCGTG	20	6.3127733E-4	46.0	33
TAACGCG	35	1.020162E-7	45.999996	38
TAGACCG	90	0.0	43.444447	42
GGCGATA	70	0.0	42.714283	12
GTGGGAT	16115	0.0	42.060814	5
TGGGATT	5640	0.0	41.962765	6
CGTTAAA	500	0.0	41.86	31
GTGGGGG	29880	0.0	41.843376	5
ATGCGTT	55	4.7293724E-11	41.81818	31
GTGGGGC	26415	0.0	41.52451	5
TTACCGG	50	8.731149E-10	41.399998	37
>>END_MODULE