##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527568_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	87684
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.003227498745495	31.0	30.0	33.0	26.0	33.0
2	30.994115231969346	31.0	31.0	34.0	28.0	34.0
3	31.580847132886273	31.0	31.0	34.0	30.0	34.0
4	35.84160166050819	37.0	35.0	37.0	35.0	37.0
5	35.964577345924	37.0	35.0	37.0	35.0	37.0
6	32.62748049815246	35.0	32.0	35.0	28.0	35.0
7	35.20025318188039	35.0	35.0	37.0	35.0	37.0
8	36.15526207745997	37.0	35.0	37.0	35.0	37.0
9	38.48906299895078	39.0	39.0	39.0	37.0	39.0
10	35.79510515031249	39.0	35.0	39.0	30.0	39.0
11	35.970005930386385	37.0	35.0	39.0	31.0	39.0
12	36.40716664385749	37.0	35.0	39.0	32.0	39.0
13	36.61735322293691	39.0	35.0	39.0	33.0	39.0
14	37.344544044523516	39.0	37.0	40.0	33.0	40.0
15	37.57872587929383	39.0	37.0	40.0	33.0	40.0
16	37.724875689977644	39.0	37.0	40.0	33.0	40.0
17	37.78221796450892	40.0	37.0	40.0	33.0	40.0
18	37.81959080333927	40.0	37.0	40.0	33.0	40.0
19	37.5861730760458	39.0	37.0	40.0	33.0	40.0
20	37.49807262442407	39.0	37.0	40.0	33.0	40.0
21	37.72079284704165	39.0	37.0	40.0	33.0	40.0
22	37.64007116463665	39.0	37.0	40.0	33.0	40.0
23	37.47230965740614	39.0	37.0	40.0	33.0	40.0
24	37.23225445919438	39.0	36.0	40.0	33.0	40.0
25	37.28735002965193	39.0	36.0	40.0	33.0	40.0
26	37.21223940513663	39.0	35.0	40.0	33.0	40.0
27	36.98691893617992	38.0	35.0	40.0	33.0	40.0
28	36.58332192874413	38.0	35.0	40.0	32.0	40.0
29	36.3910063409516	38.0	35.0	40.0	32.0	40.0
30	36.26288718580357	38.0	35.0	40.0	32.0	40.0
31	36.29015555859678	38.0	35.0	40.0	32.0	40.0
32	36.1289174763925	38.0	35.0	40.0	32.0	40.0
33	36.0580721682405	38.0	35.0	40.0	31.0	40.0
34	35.902730258656085	37.0	35.0	40.0	31.0	40.0
35	35.726095981022766	37.0	35.0	40.0	31.0	40.0
36	35.720154190046074	37.0	35.0	40.0	31.0	40.0
37	35.69622736189042	37.0	35.0	40.0	31.0	40.0
38	35.6568473153597	37.0	35.0	40.0	31.0	40.0
39	35.56770904612016	37.0	35.0	40.0	31.0	40.0
40	35.56891793257607	37.0	35.0	40.0	31.0	40.0
41	35.62197208156562	37.0	35.0	40.0	31.0	40.0
42	35.708612745768896	37.0	35.0	40.0	31.0	40.0
43	35.6059258245518	37.0	35.0	40.0	31.0	40.0
44	35.49749099037453	37.0	35.0	40.0	31.0	40.0
45	35.42188996852333	37.0	35.0	40.0	31.0	40.0
46	35.09336937183522	36.0	35.0	40.0	29.0	40.0
47	35.00388896491948	36.0	34.0	40.0	29.0	40.0
48	34.94469914693673	36.0	35.0	40.0	29.0	40.0
49	34.860430637288445	35.0	34.0	39.0	29.0	40.0
50	34.706662561014554	35.0	34.0	39.0	29.0	40.0
51	34.50705944071895	35.0	34.0	39.0	28.0	40.0
52	34.00466447698554	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	0.0
14	3.0
15	2.0
16	11.0
17	9.0
18	32.0
19	32.0
20	55.0
21	103.0
22	158.0
23	230.0
24	346.0
25	486.0
26	620.0
27	841.0
28	1088.0
29	1391.0
30	1652.0
31	2160.0
32	2897.0
33	4011.0
34	6459.0
35	8795.0
36	17954.0
37	15884.0
38	21393.0
39	1067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	98.85840062040965	0.8564846494229278	0.17106883810045162	0.11404589206696775
8	99.00095798549337	0.7116463664978787	0.18703526298982712	0.10036038501893162
9	96.92874412663656	1.836138862278181	0.2554627982300077	0.979654212855253
10	49.070525979654214	41.37356872405456	4.1410063409515985	5.414898955339629
11	30.711418274713743	27.40180648693034	29.59034715569545	12.296428082660462
12	28.379179781944252	21.730304274440034	27.65612882623968	22.23438711737603
13	23.636011130879066	22.867341818347704	33.46676702705169	20.029880023721546
14	20.668537019296565	30.532366224168605	28.50691118105926	20.29218557547557
15	22.055335066830892	35.018931618083116	24.717166187673918	18.20856712741207
16	21.862597509237716	39.76095981022764	21.234204643948726	17.142238036585923
17	21.681264540851238	44.590803339263715	17.813968340860363	15.913963779024678
18	21.264997034806807	39.28196706354637	23.4466949500479	16.006340951598926
19	19.37981843893983	42.10460289220382	23.768304365676748	14.7472743031796
20	21.367638337667078	43.88029743168651	22.0815656220063	12.670498608640116
21	20.811094384380276	38.76762009032434	23.951918251904566	16.469367273390812
22	19.31481228046166	44.01943342000821	21.92076091419187	14.74499338533826
23	17.57447196751973	42.82195155330505	24.00894119793805	15.59463528123717
24	16.950640937913416	44.0274166324529	27.049404680443413	11.972537749190275
25	18.831257698097716	45.9981296473701	21.56379727202226	13.606815382509922
26	18.572373523105696	46.98918844943205	19.16427170293326	15.27416632452899
27	18.042060124994297	45.02303727019753	22.228684822772685	14.706217782035491
28	18.251904566397517	44.01259066648419	21.60827516992838	16.12722959718991
29	16.715706400255463	44.26577254687286	19.826878335842345	19.191642717029332
30	15.676748323525386	43.38875963687788	20.838465398476348	20.09602664112039
31	18.971534145340087	43.625975092377175	22.52406368322613	14.878427079056614
32	19.070754071438348	47.440810182017245	18.652205647552574	14.836230098991834
33	20.22603895807673	45.57501938780165	17.130833447379228	17.068108206742394
34	17.75124310022353	44.337621458875056	22.252634460106748	15.65850098079467
35	15.294694585101047	48.87664796314037	21.974362483463345	13.85429496829524
36	15.139592171889968	45.80311117193558	21.894530359016468	17.162766297157976
37	11.73874367045299	49.83235253866155	18.710369052506728	19.71853473837872
38	15.188631905478763	45.838465398476345	22.461338442589298	16.51156425345559
39	16.324528990465765	43.83011723917705	21.230783267186716	18.614570503170473
40	15.016422608457644	46.79759135075955	19.606769764153096	18.579216276629715
41	17.174170886364674	45.903471556954514	21.091647278865018	15.830710277815793
42	16.576570411933762	45.2385840062041	23.493453765795355	14.691391816066785
43	18.971534145340087	43.62027279777382	19.966014324164043	17.44217873272205
44	17.68053464714201	44.927238720861276	18.038638748232287	19.35358788376443
45	16.278910633638976	46.93558688016058	18.95556772045071	17.829934765749737
46	18.17891519547466	45.39710779617718	20.97418001003604	15.44979699831212
47	17.972492130833448	46.85233337895169	22.2731627206788	12.902011769536061
48	18.273573285890244	48.49801560147803	18.023812782263583	15.20459833036814
49	15.03010811550568	44.2566488755075	21.39044751608047	19.322795492906344
50	20.5430865380229	43.70238584006204	20.943387619177958	14.811140002737103
51	17.05100132293235	46.545549929291546	20.75407143834679	15.649377309429314
52	16.90502258108663	46.65389352675516	18.363669540623146	18.077414351535058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.5
8	3.0
9	3.0
10	3.0
11	24.5
12	46.0
13	444.0
14	2259.5
15	3677.0
16	4918.0
17	6159.0
18	4996.0
19	3833.0
20	3230.0
21	2627.0
22	2139.5
23	1652.0
24	1567.0
25	1482.0
26	1409.5
27	1337.0
28	1606.0
29	1875.0
30	1555.0
31	1235.0
32	1543.0
33	1851.0
34	1710.0
35	1569.0
36	1876.0
37	2183.0
38	2595.0
39	3000.0
40	2993.0
41	3205.5
42	3418.0
43	3724.0
44	4030.0
45	3551.5
46	3073.0
47	2882.5
48	2692.0
49	3092.5
50	3493.0
51	3970.0
52	4447.0
53	4069.0
54	3691.0
55	4162.0
56	4633.0
57	4843.5
58	5054.0
59	4729.0
60	4404.0
61	3781.5
62	3159.0
63	2681.0
64	2648.5
65	3094.0
66	2415.5
67	1737.0
68	1499.0
69	1261.0
70	900.5
71	540.0
72	301.0
73	62.0
74	134.5
75	207.0
76	152.5
77	98.0
78	53.5
79	9.0
80	6.0
81	3.0
82	2.0
83	1.0
84	0.5
85	0.0
86	0.5
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	87684.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.708133753022215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.12339585389931	9.54678162492587
2	6.524275329803464	1.6582272706537111
3	2.4679170779861797	0.9408786095524838
4	1.121780489993718	0.5702294603348387
5	0.6730682939962308	0.4276720952511291
6	0.5205061473570851	0.39687970439304776
7	0.3948667324777887	0.35126134756626065
8	0.44871219599748724	0.456183568267871
9	0.5025576595171857	0.5747912960175174
>10	9.261419725388135	25.908945759773733
>50	1.3371623440725118	11.532320605811778
>100	1.5704926859912052	42.09776013867981
>500	0.03589697567979898	2.6823593814150817
>1k	0.01794848783989949	2.8557091373568726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCAGGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1263	1.4403996168058026	No Hit
GCAGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1241	1.4153095205510697	No Hit
GCAGGAGGGGAGCCGGGCGTGGTGGCGCATGCCTTTAATCCCAGCACTCGGG	759	0.8656083207882852	No Hit
GCAGGAGGGGGCAGATAGCCCTCGTCGTTAAGGTCGAACATTTCCGGGTCTT	545	0.6215501117649742	No Hit
GCAGGAGGGACCCTCACCCCCACTTCACCCGCCGCTACTGAACTGCTCTGGA	535	0.6101455225582775	No Hit
GCAGGAGGGACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG	513	0.5850554263035446	No Hit
GCAGGAGGGGAAGTAAACGTGGGTGGGAAGGAGAAAGGGTGGCTGTTTCACT	463	0.5280324802700607	No Hit
GCAGGAGGGATTCTTTTGGATTCTTTTGAACTTGTAATACTATATATAATTA	443	0.5052233018566671	No Hit
GCAGGAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	429	0.48925687696729164	No Hit
GCAGGAGGGCCTGCTGAGTGTTCTTAGACCACAATCCTCCCTGAAGCCTCAG	424	0.4835545823639433	No Hit
GCAGGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	416	0.47443091099858586	No Hit
GCAGGAGGGTGCTCCAAGTGTCTGGGTCCATCTCATCTCCTTGGTGTGTTTC	390	0.44477897906117425	No Hit
GCAGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	385	0.43907668445782577	No Hit
GCAGGAGGGGTGGAGAGGAGGATGTAGGGTTGGGAAAGGACAAAGCTTATAC	378	0.4310934720131381	No Hit
GCAGGAGGGAGGGTCCGGAGGGTGGGCTTCTCACACATGCTACTCACCGCCC	376	0.42881255417179875	No Hit
GCAGGAGGGGTCCCTGGAGAGTGAGCCCCACAGCAGCTTTCTGTGCTGCCAT	374	0.4265316363304594	No Hit
GCAGGAGGGGGTTTCTATTCCCTTTCTAACTGCCCATCGGGCTCAGAACCCC	368	0.41968888280644134	No Hit
GCAGGAGGGGTCTGGCGCATGAGGTCGGGTGGAAGTACCAGGCAGTGACAGC	364	0.4151270471237626	No Hit
GCAGGAGGGGACTCCCACAATTCAATTGGGGACACACGAAGGACGTGATTGG	345	0.3934583276310387	No Hit
GCAGGAGGGGAAGAATGGAATTGTATTAGTCAGCCTGTTAATGCCACTTCAG	344	0.392317868710369	No Hit
GCAGGAGGGGGCTCCCCTGGCATAGAGTACCCGTGTCCCCTCCCCACCCTGC	343	0.3911774097896994	No Hit
GCAGGAGGGGGACCTAGCAGAGCTGGGGATTGGGCCCCAAAGAAGTGGGGGT	342	0.3900369508690297	No Hit
GCAGGAGGGGGGCTTTGATCCCAGGGTCGGGAGCACATCTTCACTGATTGTG	335	0.3820537384243419	No Hit
GCAGGAGGGATGGTCCTCTGATCACCAATCAGATGCTGCTGTGATTTCTTTT	332	0.3786323616623329	No Hit
GCAGGAGGGGGAAGCGGGGCTTCGAGGTGGTGTATATGACTGAGCCTATTGA	330	0.37635144382099356	No Hit
GCAGGAGGGACCTAGCAGAAATAAAGAAGAGTCAATCAATAATGAATAATGC	324	0.36950869029697553	No Hit
GCAGGAGGGAGACCTACAGGGGGTCTGGCTGGTGTTAACAGAAGGGAGGGCA	319	0.3638063956936271	No Hit
GCAGGAGGCGACCAGGGAAACCCTAGAGCTTTGAGCCTGTGCTCAGAGCTGT	318	0.36266593677295744	No Hit
GCAGGAGGGGGGTTACCATGCCCTCCTCCAGGGGATCATCCTAGGGATAGAA	317	0.3615254778522878	No Hit
GCAGGAGGGATTTTCTTTATCTACCACAACGTTGTTCCTTGCGAAAATTCCA	310	0.35354226540760003	No Hit
GCAGGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	309	0.3524018064869303	No Hit
GCAGGAGGGGGTGACGATAACTGATAGTGCATGTTGAGGGGGGGAGGGGGGA	308	0.35126134756626065	No Hit
GCAGGAGGGGAGGCCCCAGTGGAGACTGGTCAGCTGCTTCTGAGCAGGGGCT	303	0.3455590529629123	No Hit
GCAGGAGGGTGCTCTCCTCACAAAAGGGAAAATAAACTGAAGTTAAGATTTA	300	0.34213767620090324	No Hit
GCAGGAGGGGTTTTGTAGAGGTGAAGTACCCCTGGATCATGTCCGAGTTTTT	299	0.3409972172802336	No Hit
GCAGGAGGATAGAAGAGGTGGCCTTCATGGTGGAGATTGATCCAGGAACTAC	296	0.33757584051822453	No Hit
GCAGGAGGGGTTTCTGAGGGGCAAAATGCTTGAGGGGGGAATGGTAATGCTC	291	0.3318735459148761	No Hit
GCAGGAGGGAGACAAACATCGCCTTGTCTTCACTGCATGTGTAGTTCCAGTC	289	0.3295926280735368	No Hit
GCAGGAGGGCCTTATTGGTTTTATTTCCTGCAGAACCCTGATACATGATCCA	288	0.3284521691528671	No Hit
GCAGGAGGGAGGGTCCAGACCATTTTTGCCCTCCGGGAAGATGAAGTGAGGG	286	0.32617125131152774	No Hit
GCAGGAGGGAAGGCAGGTCAATGTGACACATGCCTTTATTCCTAACACCTAG	282	0.32160941562884904	No Hit
GCAGGAGGGGGTGATGGACAGGGAAGCCTGGCACACAGCAGTCCTTGGGGTC	280	0.3193284977875097	No Hit
GCAGGAGGGCTGTGGTAATGCGTACAGTGGCACTGTTTTTGTGGTCTGCATC	277	0.31590712102550067	No Hit
GCAGGAGGACTGTTGAAATGGTGTCACGTGAAGCTGCCATTCCACTGATGTC	266	0.3033620728981342	No Hit
GCAGGAGGGAGGGCCAGATGGTTTTAGTGCAGAATTCTATTAGACTTCCAAA	266	0.3033620728981342	No Hit
GCAGGAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	262	0.2988002372154555	No Hit
GCAGGAGGGAGTGATGCTGGCCTTGGGTTTACAGACTACCATCCCAGTGCCT	260	0.2965193193741162	No Hit
GCAGGAGGGGGGTTGCATGTGGGTTGTGGGTGCTGTCCTGAGGATTCAGTAG	258	0.2942384015327768	No Hit
GCAGGAGGGTGGTAGCTCATAAGCCTGTCCTGAGGCATCTGGCGTCCTCTTC	257	0.2930979426121071	No Hit
GCAGGAGGGGTTAGGAGGCTTGGAGCTATTAGTTAATCTATCTTCCAATACT	257	0.2930979426121071	No Hit
GCAGGAGGGACTGGAGTCTGGCATGAACTTGAATATCACAGCCCTATCCACA	254	0.28967656585009804	No Hit
GCAGGAGGGGGCAGGCGGATTTCTGAGTTCGAGGCCAGTCTGGTCTACAGAG	253	0.28853610692942844	No Hit
GCAGGAGGGGAAGAAGAAGAGGTGGAATCGTCCCAAAATGTCTCTTGCCCAG	253	0.28853610692942844	No Hit
GCAGGAGGGAGGTATATACTCAATATACATAATTATTACCACCCAACGCGGC	252	0.2873956480087587	No Hit
GCAGGAGGGATTTGAAATGAAGTCAGAAGCTACAAGCAGAACGCTCACCTTT	251	0.28625518908808906	No Hit
GCAGGAGGGGTTTCATTAAATGAGAGATGAAGTTAGTATCCTCTTATAGATG	250	0.28511473016741934	No Hit
GCAGGAGGGATCAGTCATGATGGCAATTTCTGTATAAAAGAGCCTTAAATGG	249	0.2839742712467497	No Hit
GCAGGAGGGACCCCATCTTAAGGGATGGTTTCTTACCCATTATTCTTGCTTA	249	0.2839742712467497	No Hit
GCAGGAGGGAAAACCACTGTTTTTTGAAACCTCAAAAAATTTGCATAATGTC	247	0.2816933534054103	No Hit
GCAGGAGGGTCCTGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	246	0.2805528944847407	No Hit
GCAGGAGGGGAGCTGTGCTTACATGTTTACTTGTTTCTCACTTTTTGCTTCA	246	0.2805528944847407	No Hit
GCAGGAGGGGACCCCCAGCTCCAAAGCAAGTGTACCAGGGGAGGATGCTCAC	242	0.275991058802062	No Hit
GCAGGAGGGAGCATGTGTCATCGCACCCAGACAGCCAAATTAGCATTGATTC	241	0.27485059988139227	No Hit
GCAGGAGGGGTTACTGTCACATCAGTGTACCTTATCTGAGTAGACTTTTAAC	241	0.27485059988139227	No Hit
GCAGGAGGGGGCCGTCCCTTGAAGCACCAACTGGTGCTTCAACTATCAAGGT	240	0.2737101409607226	No Hit
GCAGGAGGGAAGAAGATATGATTATGTGTTAGATTGACACGGGATGGATTAT	239	0.27256968204005294	No Hit
GCAGGAGGGGATTTCAATTTTAACCAATTCTGTCCTCTTTTTCCAAACCCCG	239	0.27256968204005294	No Hit
GCAGGAGGGGTCTTAAAATACACAAAGCAAAACTTGAGAACTCAAAGAAGAA	238	0.2714292231193832	No Hit
GCAGGAGGGATGTACATGTATATATGTATGTGTATATATATGGAAAAGGAAA	237	0.27028876419871356	No Hit
GCAGGAGGCACCGAAGACATGCATACTCCATCGATGTTGTATTCACAGTGGC	235	0.26800784635737424	No Hit
GCAGGAGGGCATTGTTTTGTATGGATTAAAAAAAAATACTTTGCCGGGCCTG	235	0.26800784635737424	No Hit
GCAGGAGGGCAAAGGTCCTGAGTTCAAATCTTAGCAACCACATGGTGGCTCA	234	0.2668673874367045	No Hit
GCAGGAGGGATTTGACAGCGAAGACAACTCCCCCTTTCCCCCCTTTTTGGTG	230	0.2623055517540258	No Hit
GCAGGAGGGGTTTTTTCTTACCTTGTAGTCTGGTTCTGAATTATGAGAGGAA	227	0.25888417499201677	No Hit
GCAGGAGGGAGGCTGCATTGGGGCCAAACACAGAATCAGCAAGGAGGAGGCC	226	0.2577437160713471	No Hit
GCAGGAGGGCTCATTTTTCACTTCTGTGTCACAGTCCATTCATTGTACAAGT	226	0.2577437160713471	No Hit
GCAGGAGGGATTTGTATATTTGTGTTAGCCTACTTCCTGCTGTTGTAACGAA	225	0.25660325715067744	No Hit
GCAGGAGGGGTGTCACCTATTACACTGCCTTATTCCACAAGGGCCACATGCT	223	0.25432233930933806	No Hit
GCAGGAGGGATTCCTTACCTGGGCCTCTGATACCCTTTGGTACGCGTACGTG	223	0.25432233930933806	No Hit
GCAGGAGGGATGTGGGTGTCCCCTGGTGCCCCTCTCTTCTCTTTCTCTCTTT	223	0.25432233930933806	No Hit
GCAGGAGGGCTCGAACTCAGAAATCCACCTGCCTCTGCCTCCCAAGTGCTGG	222	0.2531818803886684	No Hit
GCAGGAGGGAACATGAACTGTGTGAAATTGCATACTGCTGTGACGCTAACCT	220	0.250900962547329	No Hit
GCAGGAGGGGCACAGCTGCTTCCTTGCTACAACAGCAGAGTTGAACTGCTGC	213	0.2429177501026413	No Hit
GCAGGAGGGGTGTGTGCTCGATGTAATAAAAAAATGGGGCTCCTTTGGAATC	210	0.23949637334063226	No Hit
GCAGGAGGGATTTTGTCTAGGGCTGCAATTTGTTTTATGCTTACTTTATTAT	206	0.23493453765795355	No Hit
GCAGGAGGGACTGCTGCAATTATTCAGAGAAATCTATTTTCTTCAGAAACAA	204	0.2326536198166142	No Hit
GCAGGAGGGAGTTGGAGAAGGGCTCTATGGGTAAACAACAGTTGGGTGGAGA	204	0.2326536198166142	No Hit
GCAGGAGGGAGGTGGCAGAGACCTATCTGCAGAGATTTGGCTGCATTCTAGG	204	0.2326536198166142	No Hit
GCAGGAGGGGTGGAACAGTACAGTGCCTTGGGTGGCACAGCGAAATCCAGTG	203	0.2315131608959445	No Hit
GCAGGAGGGCCATCCCAGACCCCCATAATAACAGGAGGGGCCTAGGGAGCCC	200	0.2280917841339355	No Hit
GCAGGAGGGAGTCTATTTTTGTACATGTTTTCCTTTATTAAGGGCTGCAAAA	200	0.2280917841339355	No Hit
GCAGGAGGGGTTAACAGAAATTCATATTTGGTGTGGCTTAATGTATTTTGGA	198	0.22581086629259614	No Hit
GCAGGAGGGAGGGAGGGGGTTTGGCAATCCCACTAACCACCATTAAACTGAG	198	0.22581086629259614	No Hit
GCAGGAGGGGGGGCCTCCCCAGGACACACTGACGTAGATTTGCTGTGTAGTC	197	0.22467040737192645	No Hit
GCAGGAGGATCTTGCTGTGTCATGTGTGTCTACATCTTTTTTAATGGATGCA	194	0.22124903060991744	No Hit
GCAGGAGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	193	0.22010857168924775	No Hit
GCAGGAGGGGATGGCAAAGCTCACTTTTCTATTAGTAACTCTGCCGAAGACC	191	0.21782765384790842	No Hit
GCAGGAGGGGGCGACGCGGGAGCTGCTAGCGCCCCCTTTCCCGGAGGTCTCG	189	0.21554673600656904	No Hit
GCAGGAGGGAAACCACAAACAAAATGAAAACACAACCTGTGAACTGGGAGAA	188	0.21440627708589938	No Hit
GCAGGAGGGAGCTAATTTTATTTTTTTCCCTTACATATCAAGTGATGTTCAG	185	0.21098490032389036	No Hit
GCAGGAGGGATGGCCAACTGTGAGTGGTGGCAAATGCCTTTGATCCCAGCTC	183	0.20870398248255098	No Hit
GCAGGAGGGATATGTATGTATTTTATTGTAAGACAAAGCAATTTTAAGAAGA	182	0.2075635235618813	No Hit
GCAGGAGGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	181	0.20642306464121163	No Hit
GCAGGAGGGATGTTTTCTTCATTGCTGCTTGCACTTACCTCTCCCATCGTCT	180	0.20528260572054194	No Hit
GCAGGAGGGAAACCACCTCATTTCCACCCTGCGGCCCACACCCAGGTGGGGG	180	0.20528260572054194	No Hit
GCAGGAGGGAGCCAGGGTCCCTTTGCTTTCCTGTGTTCAAGCCACCTGCCAT	180	0.20528260572054194	No Hit
GCAGGAGGGAACCCAAAACCCAAAAAAATACAAGAAACAAAAACCCAAAGCA	180	0.20528260572054194	No Hit
GCAGGAGGGGGATAAGATGAGGACGGAGATGAGTAAGAAGGAGCAAGGCACA	180	0.20528260572054194	No Hit
GCAGGAGGGGGACCGTAACAAGGATCAGGAAGTAAACTTCCAGGAGTATGTC	179	0.20414214679987225	No Hit
GCAGGAGGGGGAGGCAAAGAAGGAAGAGTCCGAGGAGTCTGAAGATGACATG	175	0.19958031111719357	No Hit
GCAGGAGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	174	0.19843985219652388	No Hit
GCAGGAGGGGTCTTTGTTTCACCATGTGCTGTGTTGACTGTTGTTTTCCCAC	173	0.1972993932758542	No Hit
GCAGGAGGGAACCAAAACAGTCCCTCTGCCCTGGACCCCCGGCACTCTGGAC	171	0.19501847543451484	No Hit
GCAGGAGGGATGGAAAAGCTTGGCTCCTCCTTACCTCAGGACGATGACACTC	171	0.19501847543451484	No Hit
GCAGGAGGGATTTGAGCGTCCAACTCTCTCTCCCCACCCCTCCTGGTGTTGT	168	0.19159709867250582	No Hit
GCAGGAGGGATGAAAAGAGTTTTCAAACTGAAAATTCAAAAATTCAGAACAC	164	0.18703526298982712	No Hit
GCAGGAGGGGATGGTTTGCTGTAACCCAAGTGACCTTATCCATAAGTGCAAT	164	0.18703526298982712	No Hit
GCAGGAGGGACTCTGTCCTAGAATGATGACGGTCCTCCTCAACACGCGTTGT	164	0.18703526298982712	No Hit
GCAGGAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	163	0.18589480406915743	No Hit
GCAGGAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	162	0.18475434514848776	No Hit
GCAGGAGGGATTGCAGAGATCAATGATGATTTTTCAAAGACCACACACTGAT	161	0.18361388622781807	No Hit
GCAGGAGGGGTTTTAAGGCGACTACTACCTGTGTTGTTCTTTATTTTGTTTC	161	0.18361388622781807	No Hit
GCAGGAGGGACTCTTTATACAGAATGCATAGTCTAGCTCCTTTCATTTTTAC	160	0.1824734273071484	No Hit
GCAGGAGGGGATCATGAACTTCTTATTGCCAAATTCATTCTTAAACTGAAGT	160	0.1824734273071484	No Hit
GCAGGAGGGAGTTTCTGTGCGGTGTTAGGTTTGTGCTTTTTTTTTTCTATCT	156	0.17791159162446968	No Hit
GCAGGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	155	0.17677113270380002	No Hit
GCAGGAGGGATTTTGACCGTGATTTATTTGGAGTGGTGGCATTGTTTTTTTA	155	0.17677113270380002	No Hit
GCAGGAGGGAGTGTTTAATGTTGATGTGGAGAGAAAGGTGTATTACTTAAAA	153	0.17449021486246066	No Hit
GCAGGAGGGACTTCTTAAGCTGTGGTTTCAGTCTCTGCTAGTTCATACCGCA	151	0.17220929702112128	No Hit
GCAGGAGGGCTGGGGCTTTGCATGGGGCCCAGGCGAGCCTTGAGCTTGGATT	151	0.17220929702112128	No Hit
GCAGGAGGGTCTTGAGTTTATTTTTTCATATGCTAGATTTTATATACTATAA	151	0.17220929702112128	No Hit
GCAGGAGGGGCGTTTGCCAGGCCCCGTGCTGGCTTCTTTGTTACTATTTGTT	151	0.17220929702112128	No Hit
GCAGGAGGGGACTGGTCTGAGGGTGTGCAGGTTCCCTCTGTGCCCATCCAGC	150	0.17106883810045162	No Hit
GCAGGAGGGGTCCTGACTCGGTGTATGGATCTGTGGTCATTTCCTCTGCAGA	149	0.16992837917978193	No Hit
GCAGGAGGGAGCATGGCTCATAGTCAAATTTACCAATTCCTGGGCTAAATGC	148	0.16878792025911227	No Hit
GCAGGAGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	148	0.16878792025911227	No Hit
GCAGGAGGATTTGTGTGCTCAGATACCAGTCACCAGATCAATAAAAGCCACC	146	0.16650700241777291	No Hit
GCAGGAGGGAAGCCAGTAAATAGCACTCCTTCATGGCTTCTTCACCAGTGCG	144	0.16422608457643356	No Hit
GCAGGAGGGGGGACTGATGTCATCTTGAGCTTTTATTTTGACCGTGATTTAT	143	0.16308562565576387	No Hit
GCAGGAGGGGTGTGTTACTGGGGTGCTGGGGGAGGAGGGAGAGCTGGGCTTG	142	0.16194516673509418	No Hit
GCAGGAGGGCGGAAGGTTCTCACCACATCCTCCCCCTTACCTTCACTACATC	142	0.16194516673509418	No Hit
GCAGGAGGGAAGTTTATTAAAGAGGATTGCTTTGTGCCCCCTACCTAGTGTG	142	0.16194516673509418	No Hit
GCAGGAGGGGAGCCTCATCCTGAGGGGTGGGGAGTCTTTGCTGTTCTTGAGG	142	0.16194516673509418	No Hit
GCAGGAGGGGAACTGAAAAGAGTTTTAGTGGCCAAGTGGTTTTTTTTCTTTA	141	0.16080470781442452	No Hit
GCAGGAGGGGAGTTACGAAGATTCCTTTGGCAGAACCCCTTGTCCCTTGGCA	141	0.16080470781442452	No Hit
GCAGGAGGGAGACTAGTACACAAGCAGAATTTGTAGCTCTAAGGAAAGAACA	140	0.15966424889375486	No Hit
GCAGGAGGGGGTTAAAGATAGGGTAGAGCTGCACTCCCCTTAGCTCAAGGAT	135	0.15396195429040646	No Hit
GCAGGAGGGGGTAAAATCCCACTTTTAGCCAATCCACACACCCAGCACTACC	133	0.1516810364490671	No Hit
GCAGGAGGGGGCTTTTAACATTTGATATGTGATTCCCCCCCTCCTCCAAACT	127	0.14483828292504902	No Hit
GCAGGAGGGAGCAGCCGTTACCTGTACATTTGCACTAACTCCGGGGGCTGCG	126	0.14369782400437936	No Hit
GCAGGAGGGACTTGGGTCAAGTTGCAATCCTCCTCTTCAAGTCGGGCTAGGT	126	0.14369782400437936	No Hit
GCAGGAGGGGGGTTCCATAAGAGAGCAGGCTGAGCAAGCCAGGGGAAGCAAG	125	0.14255736508370967	No Hit
GCAGGAGGGGGCTTTGATCTACAATGAAGCTCTGAAATAAAATGGGACCGTT	124	0.14141690616304	No Hit
GCAGGAGGGAACCATGGGCATAGAGTATTGAAAAACATGCAGTGCTTTTGTA	123	0.14027644724237034	No Hit
GCAGGAGGGAAGGTGAACCTGAGGGGAGGAAGGCGTGGCTCTCTGAAACCTT	122	0.13913598832170065	No Hit
GCAGGAGGGATGGAATTAGGTAGTTTTTTTTTTCTTCTCCTTGAAGATTTTA	122	0.13913598832170065	No Hit
GCAGGAGGGGTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	122	0.13913598832170065	No Hit
GCAGGAGGAGGGATTCCCTGTGTGGTCTGAGTCTCAGACTCATCTGCTACCC	121	0.137995529401031	No Hit
GCAGGAGGGACTGTGTCCCCGGTGTAATGTTTGTAACTGATGTCTGTTAATT	118	0.13457415263902195	No Hit
GCAGGAGGGGACGTGTCATGTTGTTCTGACCTGTATTTCACACCCCAGCTAG	114	0.13001231695634324	No Hit
GCAGGAGGGGAATGAACCTCTCAAGCCACTAGGCAGCTTTGTAACCGCCCTG	114	0.13001231695634324	No Hit
GCAGGAGGGGAGGAGGTTGGTGTGGATTCTGTGGAAGGCGAGGGGGAGGAAG	112	0.12773139911500386	No Hit
GCAGGAGGGGAGATGTGTAGCACCCAAAGGCCCTGTGCTTGGGATCCTCTTG	112	0.12773139911500386	No Hit
GCAGGAGGGGAGAGTGGAGAGGAAAGCACCTCCCCCCGTGATCCCAATATCA	110	0.1254504812736645	No Hit
GCAGGAGGGGTAATTCTTTTTTTTTTTTCTGTGTCTAGGACTCTTCCCCCCC	110	0.1254504812736645	No Hit
GCAGGAGGGAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAA	108	0.12316956343232516	No Hit
GCAGGAGGGGGTGCGGACCGGAGCTTCCTCTCCTGTTTCGGGATCCTTAGGA	108	0.12316956343232516	No Hit
GCAGGAGGGAACACAGAGAAGGATTAATTACCCCCAGGCGACAATCTCCTAA	106	0.12088864559098582	No Hit
GCAGGAGGGAGGCAGAGGAGCCTGTCCCTGCTTCTCTTCTGACTGGGAAATA	106	0.12088864559098582	No Hit
GCAGGAGGGATCCATAGTTTGTTTCCAGCCAAACAGACTGGGAGGGGGGTTC	106	0.12088864559098582	No Hit
GCAGGAGGGGAAGGAAAAAAAAAGGGATAGAGAAGGGAAAAAGGGAAAGGGA	105	0.11974818667031613	No Hit
GCAGGAGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	105	0.11974818667031613	No Hit
GCAGGAGGGGCCTTCCACCAGATGGAGCCTGCAGATGGCGAGGAGCTGCAGA	103	0.11746726882897678	No Hit
GCAGGAGGGGAAAAAGATGTCGGAAGATTCAGAGGTTGTGCTGCAGCTCCCC	103	0.11746726882897678	No Hit
GCAGGAGGGGACAGCAAGAACTTGCCCACGTTAGGAAGCCAGCTGACAGGTT	103	0.11746726882897678	No Hit
GCAGGAGGGGACCTAAGTGGCTTTATTCTGATCATCTTCCAACCCCAGATGG	103	0.11746726882897678	No Hit
GCAGGAGGGAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	102	0.1163268099083071	No Hit
GCAGGAGGGGAAGTGCAGGTTAAGTAGCAAGACCCCAACTAAAGCAAAAGCT	102	0.1163268099083071	No Hit
GCAGGAGGGGTGTAGCCAGAAAGGTACTTTTTTTCTTTTAATTTTTGTTTTA	102	0.1163268099083071	No Hit
GCAGGAGGGTGAGAAGTGAATTTTCAGTTGATGAATCAGCATCTTGTTCCCA	101	0.11518635098763744	No Hit
GCAGGAGGGAAATTTGTATGTTTTCCTTTCAAAGAGAAGGTTGCAGAGCTGT	101	0.11518635098763744	No Hit
GCAGGAGGGGTGCTGCAGGGGGGTCCTTATGAATGTTTTGACCATTTTCATG	96	0.10948405638428904	No Hit
GCAGGAGGGGAATGAAAGGACAGAGAACCCCGGGGGTAAAGGTCAGGTGGGG	96	0.10948405638428904	No Hit
GCAGGAGGGATTTATTTGGGAATAATGACCCCAATAAAGGGTTGGAAATGGC	95	0.10834359746361935	No Hit
GCAGGAGGGAACTCCCAACTCCCAAACTGCCTGCATATTTACTCTTCGTCAG	94	0.10720313854294969	No Hit
GCAGGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	94	0.10720313854294969	No Hit
GCAGGAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.10606267962228	No Hit
GCAGGAGGGGAATATCAGTAGAAATAATTTACTGGACTATCCCCTTTCTCAA	92	0.10492222070161034	No Hit
GCAGGAGGGAGTTGACCCCTGACCCAAAATAAAATCAACCACAGAGCAAACT	92	0.10492222070161034	No Hit
GCAGGAGGGATTTGTCCATCCGTCCTTTAGGTAGGGGAGAAATCTCTGAATC	92	0.10492222070161034	No Hit
GCAGGAGGGGTTTCAGTCAGGCGAGTCTTTCCCTTCCTTTCACGTTAGTGAG	91	0.10378176178094065	No Hit
GCAGGAGGGATGTACACTGATGCTGCCCCCTTTGTACAGCGGCTTGGCCTCT	91	0.10378176178094065	No Hit
GCAGGAGGGGCAGTCCACAACACACACCAGAACCCTGTCAGGATTGCTTTCA	91	0.10378176178094065	No Hit
GCAGGAGGGAAAAAAAGGCCCCACAAGGGCCATGGCATGGCTGTATGTGGGG	89	0.1015008439396013	No Hit
GCAGGAGGGAGGGGAAGCTGCTCCAGGCCTCATCAGTCAGATCCGATGCAGC	88	0.10036038501893162	No Hit
GCAGGAGGGGCTATCCTTGTTACCCATTCAAAATCAGCTGTACTGTGTGAAT	88	0.10036038501893162	No Hit
GCAGGAGGGAGCTGCTGCCTAATAAACTGTGTCCTTTGGAACAACTATAAAA	88	0.10036038501893162	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.019387801651384515	0.0	0.0	0.0	0.0
7	0.019387801651384515	0.0	0.0	0.0	0.0
8	0.019387801651384515	0.0	0.0	0.0	0.0
9	0.020528260572054195	0.0	0.0	0.0	0.0
10	0.034213767620090325	0.0	0.0	0.0	0.0
11	0.053601569271474844	0.0	0.0	0.0	0.0
12	0.07869166552620775	0.0	0.0	0.0	0.0
13	0.08439396012955613	0.0	0.0	0.0	0.0
14	0.10264130286027097	0.0	0.0	0.0	0.0
15	0.11746726882897678	0.0	0.0	0.0	0.0
16	0.13001231695634324	0.0	0.0	0.0	0.0
17	0.15966424889375486	0.0	0.0	0.0	0.0
18	0.16878792025911227	0.0	0.0	0.0	0.0
19	0.19273755759317548	0.0	0.0	0.0	0.0
20	0.20642306464121163	0.0	0.0	0.0	0.0
21	0.2178276538479084	0.0	0.0	0.0	0.0
22	0.24063683226130195	0.0	0.0	0.0	0.0
23	0.2577437160713471	0.0	0.0	0.0	0.0
24	0.27713151772273165	0.0	0.0	0.0	0.0
25	0.3090643675014826	0.0	0.0	0.0	0.0
26	0.3284521691528671	0.0	0.0	0.0	0.0
27	0.3489804297249213	0.0	0.0	0.0	0.0
28	0.3900369508690297	0.0	0.0	0.0	0.0
29	0.42196980064778067	0.0	0.0	0.0	0.0
30	0.4447789790611742	0.0	0.0	0.0	0.0
31	0.4755713699192555	0.0	0.0	0.0	0.0
32	0.5029423840153278	0.0	0.0	0.0	0.0
33	0.5348752337940788	0.0	0.0	0.0	0.0
34	0.5485607408421148	0.0	0.0	0.0	0.0
35	0.5713699192555084	0.0	0.0	0.0	0.0
36	0.5850554263035446	0.0	0.0	0.0	0.0
37	0.6067241457962684	0.0	0.0	0.0	0.0
38	0.6181287350029652	0.0	0.0	0.0	0.0
39	0.6226905706856439	0.0	0.0	0.0	0.0
40	0.6386569955750194	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACC	60	0.0	46.0	24
GCCCCAG	30	1.8416595E-6	46.0	13
CTTCATT	25	3.3912114E-5	46.0	17
GTAGATT	20	6.279441E-4	46.0	34
ATGGGTA	45	3.0377123E-10	46.0	27
TGATGGC	20	6.279441E-4	46.0	18
CTTTCCT	20	6.279441E-4	46.0	26
ACTGTTG	30	1.8416595E-6	46.0	9
CTCGAAC	45	3.0377123E-10	46.0	10
TGATGAT	20	6.279441E-4	46.0	24
CTACAAG	25	3.3912114E-5	46.0	30
CGACAAT	25	3.3912114E-5	46.0	39
ACAGCAA	20	6.279441E-4	46.0	11
CGACAAA	20	6.279441E-4	46.0	14
AGCCTAT	30	1.8416595E-6	46.0	43
GAGGTGA	40	5.509719E-9	46.0	18
AGCCTAC	25	3.3912114E-5	46.0	27
ACAGTAC	25	3.3912114E-5	46.0	15
TTTGACC	40	5.509719E-9	46.0	37
TGTGACG	25	3.3912114E-5	46.0	39
>>END_MODULE