FastQCFastQC Report
Fri 17 Jun 2016
SRR1527567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14052
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10847.71420438371762No Hit
AGATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7945.65044121833191No Hit
AGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATG4012.8536863079988612No Hit
AGATGGGGGGTACATATCATATCAGGAGACCACATCAGAACTCCTGTTTT3672.6117278679191576No Hit
AGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCT3482.4765157984628523No Hit
AGATGGGGGGCAACAAATTCTAGTCTTCAGAATGAATCCCAGGGACTTCC3472.4693993737546256No Hit
AGATGGCAGCAACGGAAACCATAACGAGCATCATCTTGATTAAGCTCATT3402.4195844007970395No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3152.241673783091375No Hit
AGATGGGGGAGCCGACTTCGTCGAGAGTGTCCTTCTGATGAATGTGGTGC2621.864503273555366No Hit
AGATGGGGGTTTCTTGGACTGGAATCAGCATTCCTTTATAAAAAAAACTG2411.7150583546826075No Hit
AGATGGGGGTGCAACACTGCAATATATTAGTTGAGGAGACTTGGGACTTT1901.3521206945630515No Hit
AGATGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1831.3023057216054654No Hit
AGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTT1821.2951892968972387No Hit
AGATGGGGGACTGTAAGAAGTCCTTAATAAGACCTAAGGAATTAGAGCCA1651.1742100768573869No Hit
AGATGGGGGGTTTGCCGAACAGCACAACGCGGTGCCATTCAGTCTGCTCT1601.138627953316254No Hit
AGATGGGGAGTCTCAAAAAAGAAGAAAAGAAGAAGAAGAAGAAGAAGAAG1581.1243951038998008No Hit
AGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTTGAATATTAGA1310.932251636777683No Hit
AGATGGGGGAGCCTGCACAGCTCTGTGCACATGTGCCCCCTCCCCCCCTC1210.8610873896954171No Hit
AGATGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.825505266154284No Hit
AGATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.7329917449473384No Hit
AGATGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.6048961001992599No Hit
AGATGGGGAGAGGAGTCGGCAACGACACCTCGGGGCCGGAAAGCTATCCA730.5194990037005408No Hit
AGATGGGGGTGTTGTCATCTGGACTCTGTCGTTAATTTTAGAAAACCAAA720.5123825789923143No Hit
AGATGGGGGGTGGTCTTTTCATGCAAAAGCAAGTTGATGACCATGGGTGT720.5123825789923143No Hit
AGATGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.5052661542840876No Hit
AGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.49814972957586107No Hit
AGATGGGGGATCCGTGGGGGAGGTGGCTGGCTGCAGGGTTCTCCGGATCT690.4910333048676345No Hit
AGATGGGGAGGGGCTGGCAGCGGGCCCGAGGCGAGCGGGACAGATTGACT650.46256760603472813No Hit
AGATGGGGGGTGGTGGCACATGCTTTTAATCCCAGCACTTTGGAGGCAGA650.46256760603472813No Hit
AGATGGGGGGCCGCGAGGTGTCTGGTCCGGTAGAGTGGTGGCGGCTGCTG520.3700540848277825No Hit
AGATGGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.3629376601195559No Hit
AGATGGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.3629376601195559No Hit
AGATGGGGCAAGCCCGAAAGAAATCAAACGTCGACAAAAAAGAAATGTAG510.3629376601195559No Hit
AGATGGGAACAGGAAGGCCCAGCAGCTCCACCCTCCTGAGTGGCAGGCCA510.3629376601195559No Hit
AGATGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.3487048107031028No Hit
AGATGGGGGTGTCTCCTGCATTGCAGGAAGATTCTTTACTGTCTGAACCT440.31312268716196984No Hit
AGATGGGAACAGGAAGGCCCAGCAGCTCCACCCTCCTGAGTGGCAGGGCA430.30600626245374324No Hit
AGATGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.30600626245374324No Hit
AGATGGGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGC430.30600626245374324No Hit
AGATGGGGGGTGGGCGAACTTCGGCTATACGGTAAGCATTGGTGCGGCCT430.30600626245374324No Hit
AGATGGGGGTTTCTTTTTTGTTATGTCTACTGCATTTGGCAACCAGTATT400.2846569883290635No Hit
AGATGGGGGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.2775405636208369No Hit
AGATGGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.2704241389126103No Hit
AGATGGGGGGGAAGGAGAATCCACGAGTTGTAAGAAAATGGCAGACAAGC360.25619128949615716No Hit
AGATGGGGGGTCTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.24907486478793053No Hit
AGATGGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.24907486478793053No Hit
AGATGGGGGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.24195844007970393No Hit
AGATGGGGGCAGAGTGCAGTGCTAGATGGGGGAAGAAAAAAAAAAAAAAA320.2277255906632508No Hit
AGATGGGGGTCTGGATGTTTGTGTGTTTATTGCTTTGATGTATGAACTGT300.2134927412467976No Hit
AGATGGGGGGGAAGGGATAGGGGACTTTTGGAGAGGAAACTAGGAAAGGG280.19925989183034445No Hit
AGATGGGGGGTGCAGTGCTAGATGGGGGAAAAAAAAAAAAAAAAAAAAAA280.19925989183034445No Hit
AGATGGGGGAGGAATGAATGCCACGTGTTCACTTGAGCTCGAACAAAGAA270.19214346712211786No Hit
AGATGGGGGTGAGCCGAGATTGTGCCACTGCGCTCCAGCCTGGGTGACAG270.19214346712211786No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGATGGGGGA250.1779106177056647No Hit
AGATGGGGGCTCCCCTTGCAGAGGTTAGAACAGCATGTCATGTGTGATGG250.1779106177056647No Hit
AGATGGGGTGGTCTTCATGCTGCTGCTCTATGTCGTCTACACTCGGCTCC240.1707941929974381No Hit
AGATGGGGAGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.1707941929974381No Hit
AGATGGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.16367776828921152No Hit
AGATGGGGGGAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.15656134358098492No Hit
AGATGGGGGATATAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.15656134358098492No Hit
AGATGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.14944491887275832No Hit
AGATGGGGGGGTCTGCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.14944491887275832No Hit
AGATGGGGGATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.14232849416453175No Hit
AGATGGGGCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.14232849416453175No Hit
AGATGGGGGGAAAGAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.13521206945630515No Hit
AGATGGGGGTAGGTAGCCTATCCCTTCTCCAGGGGATCCTCCTGACCCAG180.12809564474807858No Hit
AGATGGGGGACTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGC180.12809564474807858No Hit
AGATGGGGGAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.12809564474807858No Hit
AGATGGGGGGGGCTACATAATTATCATATATGCACATGTTGCACAGATAC170.12097922003985197No Hit
AGATGGGGGGGAAGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.12097922003985197No Hit
AGATGGGGGAGACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.12097922003985197No Hit
AGATGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1138627953316254No Hit
AGATGGGGGTTGTGTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1138627953316254No Hit
AGATGGGGGAGGGAGTCAGGGAGGGTGAGATAGAGAAAAATGTAGAAGAA160.1138627953316254No Hit
AGATGGGGGAAGTGAGTTCCAGGACAGCCAGGGCAATACAGACAAACCCT160.1138627953316254No Hit
AGATGGGGGGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1067463706233988No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTCG302.353303E-644.00000440
TGATGAA302.353303E-644.00000436
GATGGAT600.044.0000042
AGCTCTT302.353303E-644.00000419
GTGTCCT302.353303E-644.00000427
AACCGCA302.353303E-644.0000049
TCAAGCT302.353303E-644.00000416
GAGTGTC302.353303E-644.00000425
TGTCCTT302.353303E-644.00000428
TGTCTTT302.353303E-644.00000437
TTCGTCG302.353303E-644.00000417
TTCTGTC302.353303E-644.00000434
CTCTTGG302.353303E-644.00000421
TTTTCGT302.353303E-644.00000441
ATGAATG302.353303E-644.00000438
GATAACC302.353303E-644.0000046
TTCTGAT302.353303E-644.00000433
ACCGCAT302.353303E-644.00000410
CCGCATC302.353303E-644.00000411
GAATGTG302.353303E-644.00000440
Produced by FastQC (version 0.11.5)