FastQCFastQC Report
Fri 17 Jun 2016
SRR1527566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10696
Sequences flagged as poor quality0
Sequence length52
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8347.797307404637248No Hit
AGATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6315.899401645474944No Hit
AGATGGGGGGCAACAAATTCTAGTCTTCAGAATGAATCCCAGGGACTTCCCT2982.786088257292446No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2792.6084517576664172No Hit
AGATGGGGGGTACATATCATATCAGGAGACCACATCAGAACTCCTGTTTTTC2502.3373223635003737No Hit
AGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA2372.2157816005983544No Hit
AGATGGGGGAGCCGACTTCGTCGAGAGTGTCCTTCTGATGAATGTGGTGCTG2362.2064323111443533No Hit
AGATGGCAGCAACGGAAACCATAACGAGCATCATCTTGATTAAGCTCATTAG2322.169035153328347No Hit
AGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCA2182.038145100972326No Hit
AGATGGGGGTTTCTTGGACTGGAATCAGCATTCCTTTATAAAAAAAACTGAA1681.5706806282722512No Hit
AGATGGGGGACTGTAAGAAGTCCTTAATAAGACCTAAGGAATTAGAGCCAAA1391.2995512341062079No Hit
AGATGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1351.262154076290202No Hit
AGATGGGGGGTTTGCCGAACAGCACAACGCGGTGCCATTCAGTCTGCTCTTT1321.2341062079281975No Hit
AGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC1261.1780104712041886No Hit
AGATGGGGGTGCAACACTGCAATATATTAGTTGAGGAGACTTGGGACTTTCT1231.149962602842184No Hit
AGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTTGAATATTAGACA1181.1032161555721767No Hit
AGATGGGGAGTCTCAAAAAAGAAGAAAAGAAGAAGAAGAAGAAGAAGAAGAA1131.056469708302169No Hit
AGATGGGGGAGCCTGCACAGCTCTGTGCACATGTGCCCCCTCCCCCCCTCAC1020.9536275243081526No Hit
AGATGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.7572924457741212No Hit
AGATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.7479431563201197No Hit
AGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCC790.7385938668661182No Hit
AGATGGGGGGTGGTCTTTTCATGCAAAAGCAAGTTGATGACCATGGGTGTGA790.7385938668661182No Hit
AGATGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.6357516828721017No Hit
AGATGGGAACAGGAAGGCCCAGCAGCTCCACCCTCCTGAGTGGCAGGCCATC670.6264023934181002No Hit
AGATGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.5142109199700823No Hit
AGATGGGGAGAGGAGTCGGCAACGACACCTCGGGGCCGGAAAGCTATCCAAA540.5048616305160808No Hit
AGATGGGGGATCCGTGGGGGAGGTGGCTGGCTGCAGGGTTCTCCGGATCTCT510.4768137621540763No Hit
AGATGGGGGTGTTGTCATCTGGACTCTGTCGTTAATTTTAGAAAACCAAATC510.4768137621540763No Hit
AGATGGGGGGTGGGCGAACTTCGGCTATACGGTAAGCATTGGTGCGGCCTGG500.4674644727000748No Hit
AGATGGGGGGCCGCGAGGTGTCTGGTCCGGTAGAGTGGTGGCGGCTGCTGTG490.45811518324607325No Hit
AGATGGGGAGGGGCTGGCAGCGGGCCCGAGGCGAGCGGGACAGATTGACTGG450.4207180254300673No Hit
AGATGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.4207180254300673No Hit
AGATGGGGGGGAAGGAGAATCCACGAGTTGTAAGAAAATGGCAGACAAGCCG410.38332086761406137No Hit
AGATGGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.38332086761406137No Hit
AGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.3739715781600598No Hit
AGATGGGGGTTTCTTTTTTGTTATGTCTACTGCATTTGGCAACCAGTATTTT390.36462228870605834No Hit
AGATGGGGGGTGGTGGCACATGCTTTTAATCCCAGCACTTTGGAGGCAGAGG380.35527299925205685No Hit
AGATGGGGCAAGCCCGAAAGAAATCAAACGTCGACAAAAAAGAAATGTAGTG340.31787584143605085No Hit
AGATGGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.31787584143605085No Hit
AGATGGGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTC330.30852655198204937No Hit
AGATGGGGGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.2991772625280479No Hit
AGATGGGGGTGTCTCCTGCATTGCAGGAAGATTCTTTACTGTCTGAACCTCC270.2524308152580404No Hit
AGATGGGGGTGAGCCGAGATTGTGCCACTGCGCTCCAGCCTGGGTGACAGAG260.24308152580403888No Hit
AGATGGGGTGGTCTTCATGCTGCTGCTCTATGTCGTCTACACTCGGCTCCTG260.24308152580403888No Hit
AGATGGGGAGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.24308152580403888No Hit
AGATGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.2337322363500374No Hit
AGATGGGGGAGGGAGTCAGGGAGGGTGAGATAGAGAAAAATGTAGAAGAAGA250.2337322363500374No Hit
AGATGGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.2337322363500374No Hit
AGATGGGGGAGGAATGAATGCCACGTGTTCACTTGAGCTCGAACAAAGAAGA230.2150336574420344No Hit
AGATGGGGGGTCTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.2056843679880329No Hit
AGATGGGGGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.2056843679880329No Hit
AGATGGGGGGTGCAGTGCTAGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAA200.1869857890800299No Hit
AGATGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1869857890800299No Hit
AGATGGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1869857890800299No Hit
AGATGGGGGGGAAGGGATAGGGGACTTTTGGAGAGGAAACTAGGAAAGGGGA190.17763649962602843No Hit
AGATGGGGGACTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTC190.17763649962602843No Hit
AGATGGGGGTCTGGATGTTTGTGTGTTTATTGCTTTGATGTATGAACTGTTA190.17763649962602843No Hit
AGATGGGGGCTCCCCTTGCAGAGGTTAGAACAGCATGTCATGTGTGATGGGA180.16828721017202694No Hit
AGATGGGGCGGGAGCTTGAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.16828721017202694No Hit
AGATGGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.16828721017202694No Hit
AGATGGGGGAGACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15893792071802543No Hit
AGATGGGGGATATAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15893792071802543No Hit
AGATGGGGGCGTGTCTCTAGCTGCCTATGAATCAGAAATGGCCTAGTCGGCC170.15893792071802543No Hit
AGATGGGGGGGGCTACATAATTATCATATATGCACATGTTGCACAGATACAT170.15893792071802543No Hit
AGATGGGGGAAGTGAGTTCCAGGACAGCCAGGGCAATACAGACAAACCCTGT170.15893792071802543No Hit
AGATGGGGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15893792071802543No Hit
AGATGGGGGGGTCTGCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15893792071802543No Hit
AGATGGGGGCAGAGTGCAGTGCTAGATGGGGGAAGAAAAAAAAAAAAAAAAA160.14958863126402394No Hit
AGATGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA160.14958863126402394Illumina Single End Adapter 2 (97% over 34bp)
AGATGGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.14023934181002243No Hit
AGATGGGGGAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.14023934181002243No Hit
AGATGGGGGAGGAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.13089005235602094No Hit
AGATGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA140.13089005235602094Illumina Single End Adapter 2 (97% over 34bp)
AGATGGGGGAGGGTCATCACTGGTAGAGGAGTGTTGCCCGCGGGGCGAAAAA130.12154076290201944No Hit
AGATGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.12154076290201944No Hit
AGATGGGGGGAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.12154076290201944No Hit
AGATGGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA120.11219147344801794Illumina Single End Adapter 2 (97% over 34bp)
AGATGGGGGATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11219147344801794No Hit
AGATGGGGGGAGTGCAGTGCTAGATGGGGAAAAAAAAAAAAAAAAAAAAAAA110.10284218399401646No Hit
AGATGGGGCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.10284218399401646No Hit
AGATGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA110.10284218399401646Illumina Single End Adapter 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGCA358.98126E-846.0000042
TGGGGAG550.046.0000044
GGGGAGC550.046.0000046
TTAGACA206.0326623E-446.046
ACAAATT452.5283953E-1046.013
CTTTTCG301.6926806E-646.040
TTGTTTC301.6926806E-646.034
TGATGAA452.5283953E-1046.036
GATGGAT600.046.02
TCCTTTA206.0326623E-446.032
TGTTGGT301.6926806E-646.041
TTTGAAT206.0326623E-446.038
CTTCAGA452.5283953E-1046.025
GGTTGTT301.6926806E-646.032
ATGGGAG301.6926806E-646.03
GTATGCA301.6926806E-646.046
AACGCCG301.6926806E-646.018
GGGTTTC253.1931253E-546.07
AAACTGA206.0326623E-446.045
TCCTTCT452.5283953E-1046.030
Produced by FastQC (version 0.11.5)