FastQCFastQC Report
Fri 17 Jun 2016
SRR1527565_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527565_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13279
Sequences flagged as poor quality0
Sequence length52
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11428.60004518412531No Hit
AGATGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7515.655546351381881No Hit
AGATGGGGGGCAACAAATTCTAGTCTTCAGAATGAATCCCAGGGACTTCCCT4313.2457263348143686No Hit
AGATGGGGGGTACATATCATATCAGGAGACCACATCAGAACTCCTGTTTTTC3842.8917840198810154No Hit
AGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3752.8240078319150537No Hit
AGATGGGGGTTTCTTGGACTGGAATCAGCATTCCTTTATAAAAAAAACTGAA2752.070939076737706No Hit
AGATGGGGGAGCCGACTTCGTCGAGAGTGTCCTTCTGATGAATGTGGTGCTG2431.8299570750809548No Hit
AGATGGGGGTGCAACACTGCAATATATTAGTTGAGGAGACTTGGGACTTTCT1961.4760147601476015No Hit
AGATGGGGGACTGTAAGAAGTCCTTAATAAGACCTAAGGAATTAGAGCCAAA1781.3404623842156789No Hit
AGATGGGGGGTTTGCCGAACAGCACAACGCGGTGCCATTCAGTCTGCTCTTT1701.280216883801491No Hit
AGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCA1681.265155508697944No Hit
AGATGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1661.2500941335943971No Hit
AGATGGATACATCTGTCAACGCCGCTAATCAGGTTGTTTCTGTTGGTGCTGA1651.2425634460426236No Hit
AGATGGCAGCAACGGAAACCATAACGAGCATCATCTTGATTAAGCTCATTAG1541.1597258829731154No Hit
AGATGGGGAGTCTCAAAAAAGAAGAAAAGAAGAAGAAGAAGAAGAAGAAGAA1471.107011070110701No Hit
AGATGGGGGAGCCTGCACAGCTCTGTGCACATGTGCCCCCTCCCCCCCTCAC1190.8961518186610438No Hit
AGATGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.8584983809021763No Hit
AGATGGACGCCGTTGGCGCTCTCCGTCTTTCTCCATTGCGTCGTGGCCTTGC1080.8133142555915355No Hit
AGATGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.7982528804879886No Hit
AGATGGGAACAGGAAGGCCCAGCAGCTCCACCCTCCTGAGTGGCAGGCCATC1020.7681301302808946No Hit
AGATGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.5723322539347843No Hit
AGATGGGGGGTGGTCTTTTCATGCAAAAGCAAGTTGATGACCATGGGTGTGA750.5648015663830107No Hit
AGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.5422095037276903No Hit
AGATGGGAAAGGTCATGCGGCATACGCTCGGCGCCAGTTTGAATATTAGACA710.5346788161759168No Hit
AGATGGGGAGAGGAGTCGGCAACGACACCTCGGGGCCGGAAAGCTATCCAAA710.5346788161759168No Hit
AGATGGGGGTGTTGTCATCTGGACTCTGTCGTTAATTTTAGAAAACCAAATC700.5271481286241434No Hit
AGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCC670.504556065968823No Hit
AGATGGGGGATCCGTGGGGGAGGTGGCTGGCTGCAGGGTTCTCCGGATCTCT660.4970253784170495No Hit
AGATGGGGGGTGGGCGAACTTCGGCTATACGGTAAGCATTGGTGCGGCCTGG650.48949469086527597No Hit
AGATGGGGCAAGCCCGAAAGAAATCAAACGTCGACAAAAAAGAAATGTAGTG640.48196400331350253No Hit
AGATGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.4593719406581821No Hit
AGATGGGGGGTGGTGGCACATGCTTTTAATCCCAGCACTTTGGAGGCAGAGG580.4367798780028616No Hit
AGATGGGGGGCCGCGAGGTGTCTGGTCCGGTAGAGTGGTGGCGGCTGCTGTG560.42171850289931473No Hit
AGATGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.4141878153475412No Hit
AGATGGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.40665712779576774No Hit
AGATGGGGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.3991264402439943No Hit
AGATGGGGAGGGGCTGGCAGCGGGCCCGAGGCGAGCGGGACAGATTGACTGG510.3840650651404473No Hit
AGATGGGGGGGAAGGGATAGGGGACTTTTGGAGAGGAAACTAGGAAAGGGGA510.3840650651404473No Hit
AGATGGGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTC500.37653437758867386No Hit
AGATGGGGGTGTCTCCTGCATTGCAGGAAGATTCTTTACTGTCTGAACCTCC490.36900369003690037No Hit
AGATGGGGGTTTCTTTTTTGTTATGTCTACTGCATTTGGCAACCAGTATTTT460.34641162738157993No Hit
AGATGGGGGGGAAGGAGAATCCACGAGTTGTAAGAAAATGGCAGACAAGCCG430.3238195647262595No Hit
AGATGGGGGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.27863543941561864No Hit
AGATGGGGGGTGCAGTGCTAGATGGGGGAAAAAAAAAAAAAAAAAAAAAAAA350.2635740643120717No Hit
AGATGGGGGTGAGCCGAGATTGTGCCACTGCGCTCCAGCCTGGGTGACAGAG340.2560433767602982No Hit
AGATGGGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.2560433767602982No Hit
AGATGGGGGGTCTCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.24851268920852476No Hit
AGATGGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.2259206265532043No Hit
AGATGGGGGTCTGGATGTTTGTGTGTTTATTGCTTTGATGTATGAACTGTTA300.2259206265532043No Hit
AGATGGGGAGAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.2259206265532043No Hit
AGATGGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.21085925144965736No Hit
AGATGGGGGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.20332856389788387No Hit
AGATGGGGGAGGGAGTCAGGGAGGGTGAGATAGAGAAAAATGTAGAAGAAGA270.20332856389788387No Hit
AGATGGGGGCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.18826718879433693No Hit
AGATGGGGGAGGAATGAATGCCACGTGTTCACTTGAGCTCGAACAAAGAAGA230.17320581369078997No Hit
AGATGGGGGGGTCTGCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.1656751261390165No Hit
AGATGGGGCGGGAGCTTGAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.158144438587243No Hit
AGATGGAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.158144438587243No Hit
AGATGGGGGCAGAGTGCAGTGCTAGATGGGGGAAGAAAAAAAAAAAAAAAAA210.158144438587243No Hit
AGATGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA210.158144438587243Illumina Single End Adapter 2 (97% over 34bp)
AGATGGGGGGGGCTACATAATTATCATATATGCACATGTTGCACAGATACAT200.15061375103546953No Hit
AGATGGGGGAAGTGAGTTCCAGGACAGCCAGGGCAATACAGACAAACCCTGT200.15061375103546953No Hit
AGATGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGATGGGGGAAG200.15061375103546953No Hit
AGATGGGGGCTCCCCTTGCAGAGGTTAGAACAGCATGTCATGTGTGATGGGA190.14308306348369607No Hit
AGATGGGGGACTTTTCCCCTGCCGCCGCCGACTTGCGCGGAGGCGGAAGCTC190.14308306348369607No Hit
AGATGGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.13555237593192257No Hit
AGATGGGGGGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.13555237593192257No Hit
AGATGGGGGGAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.13555237593192257No Hit
AGATGGGGTGGTCTTCATGCTGCTGCTCTATGTCGTCTACACTCGGCTCCTG180.13555237593192257No Hit
AGATGGGGGAGACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.12049100082837563No Hit
AGATGGGGGAGGGTCATCACTGGTAGAGGAGTGTTGCCCGCGGGGCGAAAAA160.12049100082837563No Hit
AGATGGGGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.12049100082837563No Hit
AGATGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.12049100082837563No Hit
AGATGGGGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.12049100082837563No Hit
AGATGGGGGAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.12049100082837563No Hit
AGATGGGGGATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.11296031327660215No Hit
AGATGGGGGATATAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10542962572482868No Hit
AGATGGGAGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10542962572482868No Hit
AGATGGGGGGGAAGAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10542962572482868No Hit
AGATGGGGGAGGAAAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.10542962572482868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGAT359.21209E-846.0000042
ATGGGAG359.21209E-846.0000043
ATGGATA359.21209E-846.0000043
AGATGGA700.046.0000041
CAGCTCC206.0872047E-446.023
ACAAATT404.9240043E-946.013
CTTTTCG253.2366108E-546.040
CGACACC206.0872047E-446.023
TGATGAA404.9240043E-946.036
ACAGCAC206.0872047E-446.019
CTTCAGA404.9240043E-946.025
GGAGACT253.2366108E-546.035
ACTGCAA253.2366108E-546.016
AACGCGG206.0872047E-446.026
GGGTTTC301.725115E-646.07
GTTGAGG253.2366108E-546.030
TCCTTCT404.9240043E-946.030
CAGTCTG206.0872047E-446.040
CTGAGTG206.0872047E-446.036
TGGCAGG206.0872047E-446.041
Produced by FastQC (version 0.11.5)