FastQCFastQC Report
Fri 17 Jun 2016
SRR1527564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085627
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC199690.6471618248090258No Hit
GTGGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT193130.6259019641713014No Hit
GTGGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC190630.6177998831355831No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG143150.4639251601052234No Hit
GTGGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC99950.3239211998080131No Hit
GTGGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG86960.28182278674642136No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT66060.21408938928781734No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC63070.20439930036909842No Hit
GTGGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA60930.19746391900252364No Hit
GTGGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT60460.1959407277678086No Hit
GTGGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG59550.1929915702708072No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC55810.1808708570413728No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT51680.16748621917036635No Hit
GTGGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG49830.1614906792039349No Hit
GTGGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG49650.1609073293693632No Hit
GTGGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA45390.1471013832844994No Hit
GTGGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA44590.14450871735306958No Hit
GTGGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA43320.14039286018692473No Hit
GTGGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA38950.12623042253648936No Hit
GTGGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC38500.12477204795006006No Hit
GTGGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT38210.12383220654991675No Hit
GTGGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA36250.11748017501791369No Hit
GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG34730.11255410974819705No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC34320.11122536845833927No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG33720.10928086900976688No Hit
GTGGTAGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT33090.1072391445887659No Hit
GTGGTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC32940.10675301972662282No Hit
GTGGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT32900.10662338643005133No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG31140.10091952138090574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATTCG254.4445907E-544.037
TAGGGGG747950.042.37935
TGGTAGG3162150.042.209192
GTAGGGG2068650.042.1856774
GTAGGGA740150.042.1511844
TAGGGGA603800.042.1381265
GGTAGGG3121000.042.1320083
TAGGGAT217750.042.1005745
GTGGTAG3212600.042.032561
AGGGGGG292200.041.9144446
TAGGGGT302250.041.7435875
TAGGGGC420200.041.670165
TAGGGAG292650.041.5868765
AGGGGGT149900.041.211476
GTAGGGC198400.041.0725824
AGGGGAG301100.040.98246
AGGGATC64600.040.9349866
TAGGGAA116550.040.8477065
AGGGATA29350.040.7768336
TAGGGCA65350.040.734515
Produced by FastQC (version 0.11.5)