##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527564_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3085627
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.532781181912135	31.0	31.0	33.0	28.0	33.0
2	29.833535291206616	31.0	30.0	31.0	25.0	33.0
3	31.222586851878077	31.0	31.0	33.0	30.0	34.0
4	35.92865339848271	37.0	35.0	37.0	35.0	37.0
5	33.658664511297054	35.0	33.0	35.0	30.0	35.0
6	34.45284929124615	35.0	35.0	36.0	32.0	37.0
7	35.586443209111145	36.0	35.0	37.0	35.0	37.0
8	36.20111050363508	37.0	35.0	37.0	35.0	37.0
9	38.42033369555037	39.0	39.0	39.0	37.0	39.0
10	36.52690879357745	39.0	37.0	39.0	32.0	39.0
11	35.5711393502844	37.0	35.0	39.0	31.0	39.0
12	35.382211135694625	37.0	35.0	39.0	31.0	39.0
13	35.849853206495794	37.0	35.0	39.0	32.0	39.0
14	36.50929778615497	38.0	35.0	40.0	31.0	40.0
15	36.71273423521378	38.0	36.0	40.0	32.0	40.0
16	36.88184735225612	38.0	36.0	40.0	32.0	40.0
17	36.808263928206486	38.0	36.0	40.0	32.0	40.0
18	36.668791788508464	38.0	35.0	40.0	32.0	40.0
19	36.661946502283	38.0	35.0	40.0	32.0	40.0
20	36.53153540593208	38.0	35.0	40.0	31.0	40.0
21	36.4448697136757	38.0	35.0	40.0	31.0	40.0
22	36.28689533764126	38.0	35.0	40.0	31.0	40.0
23	36.06476576721684	38.0	34.0	40.0	31.0	40.0
24	35.990728626629206	38.0	34.0	39.0	30.0	40.0
25	35.75662774534965	38.0	34.0	39.0	30.0	40.0
26	35.238566424263205	37.0	34.0	39.0	29.0	40.0
27	35.06613080582974	37.0	34.0	39.0	29.0	40.0
28	34.84809667532725	37.0	33.0	39.0	29.0	40.0
29	34.57887132825841	36.0	33.0	38.0	27.0	40.0
30	34.287623876767995	36.0	33.0	38.0	27.0	40.0
31	34.239818357824845	36.0	33.0	38.0	27.0	40.0
32	34.24189508323592	36.0	33.0	38.0	27.0	40.0
33	33.74841191109619	36.0	32.0	38.0	26.0	39.0
34	33.13672164522802	35.0	31.0	38.0	24.0	39.0
35	32.99527097734107	35.0	31.0	38.0	24.0	39.0
36	32.82052658989567	35.0	31.0	38.0	24.0	39.0
37	32.70788076459014	34.0	31.0	38.0	24.0	39.0
38	32.32620987565898	34.0	30.0	37.0	23.0	38.0
39	31.553714366642502	34.0	29.0	36.0	22.0	38.0
40	31.193324079676515	33.0	29.0	36.0	21.0	38.0
41	30.964553395468734	33.0	29.0	36.0	21.0	38.0
42	30.67892651963442	33.0	28.0	36.0	21.0	38.0
43	30.286069249458862	32.0	28.0	35.0	20.0	38.0
44	29.804129922378824	32.0	27.0	35.0	19.0	37.0
45	29.247982986926157	31.0	26.0	34.0	19.0	37.0
46	29.021546026139905	31.0	26.0	34.0	18.0	37.0
47	28.43160109760512	31.0	25.0	34.0	18.0	37.0
48	27.87840882906456	30.0	24.0	33.0	15.0	36.0
49	26.863900594595524	29.0	23.0	33.0	13.0	35.0
50	30.304485603736293	32.0	27.0	35.0	20.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	53.0
12	106.0
13	256.0
14	443.0
15	790.0
16	1139.0
17	1706.0
18	2710.0
19	3931.0
20	5774.0
21	8551.0
22	12186.0
23	16710.0
24	23969.0
25	31801.0
26	42615.0
27	57580.0
28	75213.0
29	96345.0
30	128531.0
31	177351.0
32	255012.0
33	335071.0
34	435469.0
35	636546.0
36	609993.0
37	123544.0
38	2229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.48405947964547	0.4064327930757671	0.07178443797646314	0.037723289302303877
8	98.70969498257567	1.0745628036052315	0.11854964971462849	0.09719256410447537
9	98.2622980677833	1.0519417933535065	0.3341946385613037	0.35156550030188355
10	66.12717609743498	23.593357200983785	4.044137544816661	6.235329156764573
11	36.42744246145111	25.45265516538454	19.434623821997928	18.685278551166423
12	31.199979777205733	17.088325970702225	26.8483520529215	24.86334219917054
13	24.384995334821742	17.02989376227263	30.219595563559693	28.365515339345944
14	25.94532650900449	16.63269734157758	30.86646571345143	26.5555104359665
15	26.38267684331256	16.39115810174075	28.713256657398965	28.512908397547726
16	31.602296713115358	17.149772153277112	25.73548261017939	25.512448523428137
17	32.48996071138864	17.4474426105294	23.115593686469555	26.9470029916124
18	30.757703377627948	19.16433839864637	23.488419047409163	26.589539176316517
19	32.14374258457033	19.007708968063866	21.716364291601025	27.132184155764776
20	30.30602856404873	20.598601191913346	22.56218914340586	26.53318110063206
21	32.33958608736571	18.81867121333849	24.32293339408814	24.51880930520766
22	32.897819470726695	17.70926946127967	24.000600202163124	25.39231086583051
23	32.58135218547154	19.133842165627925	23.031656126939517	25.253149521961017
24	32.65368756495843	18.66456963203913	22.150214526901664	26.531528276100776
25	30.17150161053167	18.68479242630428	22.67474325315406	28.468962710009993
26	29.243229982107366	18.445294910888453	22.7923206531444	29.51915445385978
27	28.41694734976068	18.101053691842857	22.99017347203664	30.491825486359826
28	31.10800495328826	19.866075841311993	21.049854697278704	27.97606450812104
29	31.240749448977468	20.660954807564234	21.078147164255434	27.020148579202864
30	31.181020907582152	18.759623246750174	22.502201335417404	27.557154510250264
31	32.89198597238098	20.13162964933869	20.530349261268455	26.446035117011874
32	35.55737618318741	19.815486447324968	19.819180996277254	24.80795637321037
33	34.92654815374638	19.173315504433944	21.7844541806252	24.115682161194467
34	31.314737652995646	21.32315409477555	24.056666602930296	23.305441649298505
35	30.2447768314187	23.53437405104376	24.04418939813529	22.17665971940225
36	31.344261636289804	23.2333979447289	20.829186418189884	24.59315400079141
37	31.416240524211126	24.175799602479497	20.000570386504915	24.407389486804465
38	32.54816606154924	22.747564757503095	20.717863824759117	23.98640535618855
39	32.50062305003165	21.641306612886133	20.138435397408696	25.719634939673526
40	29.9421803088967	21.329732984576555	21.092504051850728	27.635582654676018
41	27.567784440569127	22.077814330766486	21.999029694775164	28.355371533889222
42	27.18627364875923	19.95257365844932	22.041970724264466	30.819181968526983
43	27.376251244884752	18.72890015546273	24.926992147787143	28.967856451865376
44	27.908428335634866	18.581183014019516	24.19378622237879	29.316602427966824
45	29.02670996850883	19.06046971976846	23.89909733094765	28.013722980775057
46	28.873094512071614	21.000885719498825	22.93423670456604	27.191783063863518
47	27.321448768759154	20.828570660031172	23.81503013812104	28.03495043308864
48	29.174880826490046	20.380104270542095	24.363735474184015	26.081279428783844
49	29.307009564020536	20.013079999624065	24.047170963956436	26.632739472398963
50	26.965670186318697	20.4480645262697	23.67846146018297	28.90780382722863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	7.0
6	14.0
7	16.0
8	18.0
9	17.0
10	16.0
11	239.0
12	462.0
13	923.5
14	1385.0
15	1828.5
16	2272.0
17	2242.5
18	2213.0
19	2623.5
20	3034.0
21	3243.0
22	3452.0
23	3973.5
24	4495.0
25	4782.0
26	5069.0
27	6358.5
28	7648.0
29	9298.5
30	10949.0
31	12695.5
32	14442.0
33	17099.0
34	19756.0
35	22553.5
36	25351.0
37	28947.5
38	32544.0
39	37583.0
40	42622.0
41	45600.5
42	48579.0
43	52840.0
44	57101.0
45	64969.5
46	72838.0
47	80849.0
48	88860.0
49	99473.5
50	110087.0
51	119972.0
52	129857.0
53	143260.0
54	156663.0
55	180365.0
56	204067.0
57	244964.0
58	285861.0
59	301835.5
60	317810.0
61	321093.0
62	324376.0
63	327832.0
64	331288.0
65	284947.5
66	238607.0
67	203869.5
68	169132.0
69	144158.5
70	119185.0
71	104804.0
72	90423.0
73	74785.5
74	59148.0
75	63683.0
76	68218.0
77	46846.0
78	25474.0
79	16388.0
80	7302.0
81	5208.5
82	3115.0
83	2207.0
84	1299.0
85	810.0
86	321.0
87	230.0
88	139.0
89	99.5
90	60.0
91	48.5
92	37.0
93	37.5
94	38.0
95	19.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3085627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.306563831372406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.09298661001458	9.8592305578318
2	8.655947004204997	2.3036182266486107
3	3.0323942906255983	1.2105224457029629
4	1.5181799134017175	0.8080703170074954
5	0.8664216493527362	0.5764547490997572
6	0.5563440529988867	0.44418165920604713
7	0.42445727734241323	0.39536474982531533
8	0.3700704787702018	0.39394931582897896
9	0.3053626905703463	0.36569953204145406
>10	7.252419945313073	25.5283088334129
>50	1.873639026542912	16.973525622357652
>100	0.9669327436372882	24.449757376271855
>500	0.05217437898335091	4.723477633718448
>1k	0.02950046662142738	7.649563622410535
>5k	0.0021942495834119543	1.9610297775735697
>10k+	9.752220370719796E-4	2.357245581062676
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	19969	0.6471618248090258	No Hit
GTGGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	19313	0.6259019641713014	No Hit
GTGGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	19063	0.6177998831355831	No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	14315	0.4639251601052234	No Hit
GTGGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	9995	0.3239211998080131	No Hit
GTGGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	8696	0.28182278674642136	No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	6606	0.21408938928781734	No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	6307	0.20439930036909842	No Hit
GTGGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	6093	0.19746391900252364	No Hit
GTGGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	6046	0.1959407277678086	No Hit
GTGGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	5955	0.1929915702708072	No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	5581	0.1808708570413728	No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	5168	0.16748621917036635	No Hit
GTGGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	4983	0.1614906792039349	No Hit
GTGGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	4965	0.1609073293693632	No Hit
GTGGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	4539	0.1471013832844994	No Hit
GTGGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA	4459	0.14450871735306958	No Hit
GTGGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	4332	0.14039286018692473	No Hit
GTGGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	3895	0.12623042253648936	No Hit
GTGGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3850	0.12477204795006006	No Hit
GTGGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	3821	0.12383220654991675	No Hit
GTGGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	3625	0.11748017501791369	No Hit
GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	3473	0.11255410974819705	No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3432	0.11122536845833927	No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	3372	0.10928086900976688	No Hit
GTGGTAGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3309	0.1072391445887659	No Hit
GTGGTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	3294	0.10675301972662282	No Hit
GTGGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	3290	0.10662338643005133	No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	3114	0.10091952138090574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0373343894125894	0.0	0.0	0.0	0.0
7	0.039214072212876025	0.0	0.0	0.0	0.0
8	0.03960297210259049	0.0	0.0	0.0	0.0
9	0.040218730261305076	0.0	0.0	0.0	0.0
10	0.04725133660030846	0.0	0.0	0.0	0.0
11	0.06556203974103156	0.0	0.0	0.0	0.0
12	0.07334003753532102	0.0	0.0	0.0	0.0
13	0.07959484409489546	0.0	0.0	0.0	0.0
14	0.08834509161347111	0.0	0.0	0.0	0.0
15	0.09528047298004587	0.0	0.0	0.0	0.0
16	0.10253993758804937	0.0	0.0	0.0	0.0
17	0.1089243774441953	0.0	0.0	0.0	0.0
18	0.11540604227276985	0.0	0.0	0.0	0.0
19	0.12318404006705931	0.0	0.0	0.0	0.0
20	0.12992497148877682	0.0	0.0	0.0	0.0
21	0.13530475329649372	0.0	0.0	0.0	0.0
22	0.14039286018692473	0.0	0.0	0.0	0.0
23	0.14619395020849896	0.0	0.0	0.0	0.0
24	0.15293488163021648	0.0	0.0	0.0	0.0
25	0.15912487154150518	0.0	0.0	0.0	0.0
26	0.16560653637007972	0.0	0.0	0.0	0.0
27	0.17283359265394035	0.0	0.0	0.0	0.0
28	0.18605618890423242	0.0	0.0	0.0	0.0
29	0.19237581211209262	0.0	0.0	0.0	0.0
30	0.20076956806509666	0.0	0.0	0.0	0.0
31	0.20793180770067154	0.0	0.0	0.0	0.0
32	0.2188858212609625	0.0	0.0	0.0	0.0
33	0.22494617787567972	0.0	0.0	0.0	0.0
34	0.23314548388382653	0.0	0.0	0.0	0.0
35	0.24766441309983353	0.0	0.0	0.0	0.0
36	0.25787303520483845	0.0	0.0	0.0	0.0
37	0.2671742242338429	0.0	0.0	0.0	0.0
38	0.27466054711084653	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATTCG	25	4.4445907E-5	44.0	37
TAGGGGG	74795	0.0	42.3793	5
TGGTAGG	316215	0.0	42.20919	2
GTAGGGG	206865	0.0	42.185677	4
GTAGGGA	74015	0.0	42.151184	4
TAGGGGA	60380	0.0	42.138126	5
GGTAGGG	312100	0.0	42.132008	3
TAGGGAT	21775	0.0	42.100574	5
GTGGTAG	321260	0.0	42.03256	1
AGGGGGG	29220	0.0	41.914444	6
TAGGGGT	30225	0.0	41.743587	5
TAGGGGC	42020	0.0	41.67016	5
TAGGGAG	29265	0.0	41.586876	5
AGGGGGT	14990	0.0	41.21147	6
GTAGGGC	19840	0.0	41.072582	4
AGGGGAG	30110	0.0	40.9824	6
AGGGATC	6460	0.0	40.934986	6
TAGGGAA	11655	0.0	40.847706	5
AGGGATA	2935	0.0	40.776833	6
TAGGGCA	6535	0.0	40.73451	5
>>END_MODULE