FastQCFastQC Report
Fri 17 Jun 2016
SRR1527563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796582
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG179540.6419979818220958No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC167450.5987666372736433No Hit
GTGGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT166050.5937605262423916No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT127880.4572724847689072No Hit
GTGGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC96630.3455289349641813No Hit
GTGGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG83110.29718420557666464No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA71900.2570995593907134No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC56750.20292628644538227No Hit
GTGGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT56030.20035171505788135No Hit
GTGGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC55590.19877836587663084No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT53220.19030373505944043No Hit
GTGGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC52980.1894455445969401No Hit
GTGGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG52240.1867994573375642No Hit
GTGGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA48830.17460600118287253No Hit
GTGGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT45490.16266285057974342No Hit
GTGGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA44450.15894402524224213No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41760.14932514047505133No Hit
GTGGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG40920.14632147385630032No Hit
GTGGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG40060.14324629136567424No Hit
GTGGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC39040.139598981900048No Hit
GTGGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA36600.130874045531295No Hit
GTGGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG35580.12722673606566873No Hit
GTGGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG33710.12054000204535394No Hit
GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG32850.11746481955472789No Hit
GTGGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC32010.11446115293597685No Hit
GTGGTAGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG30950.11067081172660054No Hit
GTGGTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT30400.10870412525003736No Hit
GTGGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA30000.10727380781253687No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT29470.10537863720784874No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT28680.10255376026878525No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC206.313015E-446.038
CGAATTA206.313015E-446.045
TAGGGGG681600.044.2048155
TGGTAGG2871650.044.0969852
GTAGGGG1881650.044.076054
GTAGGGA668450.044.0387464
GGTAGGG2835100.044.03273
GTGGTAG2916050.043.9177321
TAGGGGA545900.043.914455
TAGGGAT195150.043.9139145
TAGGGAG267300.043.8488585
TAGGGGT275200.043.634815
TAGGGGC385900.043.5980835
AGGGGGT131250.043.213716
AGGGATC55450.043.096486
GTAGGGC179700.043.0562064
AGGGGAG273700.042.7815136
AGGGATA27800.042.690656
AGGGGGG274600.042.6831786
GTAGGGT110950.042.6002734
Produced by FastQC (version 0.11.5)