##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2796582
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.007934328405174	31.0	31.0	33.0	30.0	33.0
2	30.436299740182836	31.0	30.0	31.0	28.0	33.0
3	31.54557277419364	31.0	31.0	33.0	30.0	34.0
4	36.21540258787334	37.0	35.0	37.0	35.0	37.0
5	33.94367946300162	35.0	33.0	35.0	32.0	37.0
6	34.88670813156918	35.0	35.0	37.0	33.0	37.0
7	35.861998325098284	37.0	35.0	37.0	35.0	37.0
8	36.41893926228518	37.0	37.0	37.0	35.0	37.0
9	38.60431984472474	39.0	39.0	39.0	39.0	39.0
10	37.39449477969893	39.0	37.0	39.0	34.0	39.0
11	36.995571737213496	39.0	37.0	39.0	34.0	39.0
12	36.82463843363077	38.0	35.0	39.0	33.0	39.0
13	36.796376791383196	39.0	35.0	39.0	33.0	39.0
14	37.47634254958374	39.0	37.0	40.0	33.0	40.0
15	37.57126628148218	39.0	37.0	40.0	33.0	40.0
16	37.642823990142254	39.0	37.0	40.0	33.0	40.0
17	37.478154404197696	39.0	36.0	40.0	33.0	40.0
18	37.28751454454044	39.0	36.0	40.0	33.0	40.0
19	37.207851942120776	39.0	36.0	40.0	32.0	40.0
20	37.06949233028032	39.0	36.0	40.0	32.0	40.0
21	37.0059765814126	39.0	36.0	40.0	32.0	40.0
22	37.02261224594881	39.0	36.0	40.0	32.0	40.0
23	36.8404588172276	38.0	35.0	40.0	32.0	40.0
24	36.75758086120843	38.0	35.0	40.0	31.0	40.0
25	36.74969230296126	38.0	35.0	40.0	32.0	40.0
26	36.66352855020879	38.0	35.0	40.0	31.0	40.0
27	36.58746426888251	38.0	35.0	40.0	31.0	40.0
28	36.37940063978099	38.0	35.0	40.0	31.0	40.0
29	36.306636100783024	38.0	35.0	40.0	31.0	40.0
30	36.084475620596855	38.0	35.0	40.0	30.0	40.0
31	35.99039434566911	38.0	34.0	40.0	30.0	40.0
32	35.903735703083264	38.0	34.0	40.0	30.0	40.0
33	35.63231258729406	38.0	34.0	40.0	30.0	40.0
34	35.071743292347584	38.0	33.0	39.0	27.0	40.0
35	34.89505689445187	37.0	33.0	39.0	26.0	40.0
36	34.863100384683875	37.0	33.0	39.0	27.0	40.0
37	34.66171705317419	37.0	33.0	39.0	26.0	40.0
38	34.90681624926428	37.0	33.0	39.0	27.0	40.0
39	34.86875729014919	37.0	33.0	39.0	27.0	40.0
40	34.5952770203055	37.0	33.0	39.0	26.0	40.0
41	34.45899101117006	36.0	33.0	39.0	26.0	40.0
42	34.327305618072344	36.0	33.0	39.0	26.0	40.0
43	34.293855141740885	36.0	33.0	39.0	26.0	40.0
44	34.13325945743769	36.0	33.0	39.0	26.0	40.0
45	33.911306373279956	35.0	32.0	39.0	25.0	40.0
46	33.66641135500407	35.0	32.0	39.0	24.0	40.0
47	33.51096767411075	35.0	32.0	38.0	24.0	40.0
48	33.28574345397346	35.0	31.0	38.0	23.0	40.0
49	33.05189048631508	35.0	31.0	38.0	24.0	40.0
50	33.90359374407759	35.0	32.0	39.0	26.0	40.0
51	34.142128855867625	35.0	33.0	39.0	26.0	40.0
52	33.77512871069041	35.0	32.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	12.0
12	39.0
13	78.0
14	129.0
15	220.0
16	358.0
17	630.0
18	1017.0
19	1579.0
20	2349.0
21	3722.0
22	5408.0
23	7882.0
24	11145.0
25	15085.0
26	20904.0
27	27581.0
28	37176.0
29	49931.0
30	65434.0
31	88934.0
32	127317.0
33	197531.0
34	259810.0
35	315625.0
36	455716.0
37	622856.0
38	469874.0
39	8233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.53614805501859	0.3669121806548136	0.07173041949064965	0.025209344835946165
8	98.75630323015739	1.0469566063144224	0.11796543065785306	0.07877473287033958
9	98.29316644389473	1.0207817972081634	0.34374103816730567	0.3423107207298052
10	66.16119248425399	23.565123425667476	4.052339605990456	6.2213444840880765
11	36.19922462491713	25.341541925107148	19.38151643685041	19.07771701312531
12	31.05094004037786	17.047917779632424	27.12039196419057	24.780750215799145
13	24.550933961528752	16.868484457097985	30.13174653916817	28.44883504220509
14	25.989439966358933	16.516519093665053	31.03399077874348	26.460050161232534
15	26.35864065491375	16.335047568782176	28.875713281427114	28.430598494876964
16	31.535424314395215	16.959953257226143	25.850019774138573	25.654602654240065
17	32.459409379020535	17.36348156428097	23.075561524746995	27.101547531951503
18	30.872937035280923	19.01750064900654	23.474190994578382	26.635371321134155
19	32.13143759060167	18.850368056434604	21.79607106103093	27.222123291932792
20	30.454855248299534	20.41602928145858	22.587286909520266	26.541828560721626
21	32.35707016636737	18.645761147000158	24.42256297151308	24.574605715119386
22	32.82868158344722	17.610497385737304	24.015458870864506	25.54536215995097
23	32.56410861544557	19.019324303739353	23.044809699840734	25.371757380974348
24	32.61610065429871	18.487997133643855	22.27329647405297	26.622605738004463
25	30.238162156518207	18.509344621398547	22.709722082170305	28.542771139912936
26	29.284033151897564	18.22517630450314	22.85268231004848	29.638108233550813
27	28.52070849343949	17.99850674859525	22.9846290936579	30.496155664307356
28	31.077293639163805	19.708379729255213	21.149782126896334	28.064544504684648
29	31.223364807468545	20.45139388010078	21.226840478841673	27.098400833589004
30	31.223686628891983	18.6130068776814	22.508369144906172	27.65493734852044
31	32.96770843837227	19.888707000188084	20.642913384982094	26.500671176457548
32	35.6065725946888	19.507491645158268	19.986504954977182	24.899430805175747
33	34.97480138254483	18.87357495685805	21.917469253538783	24.23415440705833
34	31.390104062745166	21.10107981814944	24.049536183812954	23.45927993529244
35	30.411266324391704	23.282206636529878	24.090407504589532	22.216119534488886
36	31.569644659087416	22.957631852025077	20.800355576914964	24.672367911972543
37	31.694511371381207	23.91451421771291	19.954859181672486	24.436115229233398
38	32.718153803464375	22.5065812481093	20.76545583143995	24.00980911698638
39	32.45740693460803	21.417752098812052	20.220183066328826	25.904657900251095
40	30.028584893988448	21.175921178066652	21.182965491446343	27.612528436498557
41	27.60941749607199	21.854320738673138	22.058856132235707	28.477405633019163
42	27.34781243675315	19.751611073803666	22.28477477148891	30.615801717954273
43	27.481261053671947	18.525364176698556	25.06205789781955	28.931316871809948
44	28.02528229102526	18.378005722700067	24.23944658157708	29.357265404697593
45	29.03530094951623	18.80313182306115	23.99529139499575	28.16627583242687
46	28.84671359538179	20.794276727805585	22.955093038573516	27.403916638239107
47	27.407242126281297	20.552982176099253	23.97741242702699	28.06236327059246
48	29.18176545511628	20.050690449985016	24.575070568286574	26.19247352661213
49	29.40793439992105	19.67401635281926	24.274811180219285	26.643238067040407
50	26.953295129554576	20.511502970411737	23.67203965412064	28.86316224591305
51	26.038571370337078	21.34777381818234	23.706295756748773	28.907359054731813
52	27.79714665974393	20.335359377983554	24.313036413736484	27.554457548536032
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	6.0
6	12.0
7	13.0
8	14.0
9	12.5
10	11.0
11	255.0
12	499.0
13	962.5
14	1854.0
15	2282.0
16	2117.0
17	1952.0
18	2297.0
19	2642.0
20	2628.5
21	2615.0
22	2855.5
23	3096.0
24	3403.5
25	3711.0
26	4503.5
27	5296.0
28	6604.0
29	7912.0
30	9636.5
31	11361.0
32	12373.5
33	13386.0
34	16274.0
35	19162.0
36	22091.5
37	25021.0
38	27114.5
39	34720.5
40	40233.0
41	41595.0
42	42957.0
43	47929.0
44	52901.0
45	57395.0
46	61889.0
47	72184.0
48	82479.0
49	87660.0
50	92841.0
51	101167.0
52	109493.0
53	125404.5
54	141316.0
55	157054.0
56	172792.0
57	199018.0
58	225244.0
59	260827.5
60	296411.0
61	287478.5
62	278546.0
63	283159.0
64	252098.5
65	216425.0
66	194999.5
67	173574.0
68	143885.5
69	114197.0
70	102929.5
71	91662.0
72	80370.5
73	69079.0
74	67431.0
75	65783.0
76	48828.5
77	31874.0
78	21867.5
79	11861.0
80	8148.5
81	4436.0
82	3132.5
83	1829.0
84	1294.5
85	760.0
86	559.5
87	359.0
88	265.0
89	111.5
90	52.0
91	41.5
92	31.0
93	20.0
94	9.0
95	4.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2796582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.879655849000189
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.69101256812334	6.588842523944048
2	9.56158975605328	1.8893043231826416
3	3.6223104987102954	1.0736154331643388
4	1.719359372216222	0.6794671551299716
5	1.08856953908446	0.5377346206929612
6	0.7525943829194838	0.4461224098281099
7	0.5882814377803793	0.4068412703337616
8	0.5189730892169704	0.41018204130849106
9	0.480866117794794	0.4275712577931616
>10	10.94263701140915	28.2406265611502
>50	2.5689945757239365	17.235120413043497
>100	1.3461812531213464	24.94871507134278
>500	0.073003321751554	4.849669499426267
>1k	0.040881860180870234	7.868848411989225
>5k	0.0032851494788199297	2.086028645109402
>10k+	0.0014600664350310798	2.311310362561186
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	17954	0.6419979818220958	No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	16745	0.5987666372736433	No Hit
GTGGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	16605	0.5937605262423916	No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	12788	0.4572724847689072	No Hit
GTGGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	9663	0.3455289349641813	No Hit
GTGGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	8311	0.29718420557666464	No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7190	0.2570995593907134	No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	5675	0.20292628644538227	No Hit
GTGGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	5603	0.20035171505788135	No Hit
GTGGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	5559	0.19877836587663084	No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5322	0.19030373505944043	No Hit
GTGGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5298	0.1894455445969401	No Hit
GTGGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	5224	0.1867994573375642	No Hit
GTGGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	4883	0.17460600118287253	No Hit
GTGGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4549	0.16266285057974342	No Hit
GTGGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4445	0.15894402524224213	No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4176	0.14932514047505133	No Hit
GTGGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	4092	0.14632147385630032	No Hit
GTGGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	4006	0.14324629136567424	No Hit
GTGGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3904	0.139598981900048	No Hit
GTGGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	3660	0.130874045531295	No Hit
GTGGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3558	0.12722673606566873	No Hit
GTGGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3371	0.12054000204535394	No Hit
GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3285	0.11746481955472789	No Hit
GTGGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	3201	0.11446115293597685	No Hit
GTGGTAGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3095	0.11067081172660054	No Hit
GTGGTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	3040	0.10870412525003736	No Hit
GTGGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	3000	0.10727380781253687	No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2947	0.10537863720784874	No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	2868	0.10255376026878525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.04398226120314012	0.0	0.0	0.0	0.0
7	0.04541257864064061	0.0	0.0	0.0	0.0
8	0.04587743180782827	0.0	0.0	0.0	0.0
9	0.046342284975015934	0.0	0.0	0.0	0.0
10	0.05442357849689371	0.0	0.0	0.0	0.0
11	0.07459105436565064	0.0	0.0	0.0	0.0
12	0.08513964546721677	0.0	0.0	0.0	0.0
13	0.09329245486096957	0.0	0.0	0.0	0.0
14	0.10201739122972257	0.0	0.0	0.0	0.0
15	0.10952655777660016	0.0	0.0	0.0	0.0
16	0.11789391478597802	0.0	0.0	0.0	0.0
17	0.12472368055004288	0.0	0.0	0.0	0.0
18	0.13076677172348244	0.0	0.0	0.0	0.0
19	0.13916988666879784	0.0	0.0	0.0	0.0
20	0.1471439063828631	0.0	0.0	0.0	0.0
21	0.15422397769849053	0.0	0.0	0.0	0.0
22	0.15994524744849248	0.0	0.0	0.0	0.0
23	0.1652374219672443	0.0	0.0	0.0	0.0
24	0.17210294566724665	0.0	0.0	0.0	0.0
25	0.17868240587974893	0.0	0.0	0.0	0.0
26	0.18579823513131385	0.0	0.0	0.0	0.0
27	0.19380801278131662	0.0	0.0	0.0	0.0
28	0.20639480623132095	0.0	0.0	0.0	0.0
29	0.21433306800944868	0.0	0.0	0.0	0.0
30	0.22359437341726435	0.0	0.0	0.0	0.0
31	0.23106778202820444	0.0	0.0	0.0	0.0
32	0.24186667868133313	0.0	0.0	0.0	0.0
33	0.24833886508602288	0.0	0.0	0.0	0.0
34	0.257564412557901	0.0	0.0	0.0	0.0
35	0.2725827456516562	0.0	0.0	0.0	0.0
36	0.28516953910166054	0.0	0.0	0.0	0.0
37	0.29507448735635144	0.0	0.0	0.0	0.0
38	0.3037636657891669	0.0	0.0	0.0	0.0
39	0.31288193945323256	0.0	0.0	0.0	0.0
40	0.3202480742563601	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAC	20	6.313015E-4	46.0	38
CGAATTA	20	6.313015E-4	46.0	45
TAGGGGG	68160	0.0	44.204815	5
TGGTAGG	287165	0.0	44.096985	2
GTAGGGG	188165	0.0	44.07605	4
GTAGGGA	66845	0.0	44.038746	4
GGTAGGG	283510	0.0	44.0327	3
GTGGTAG	291605	0.0	43.917732	1
TAGGGGA	54590	0.0	43.91445	5
TAGGGAT	19515	0.0	43.913914	5
TAGGGAG	26730	0.0	43.848858	5
TAGGGGT	27520	0.0	43.63481	5
TAGGGGC	38590	0.0	43.598083	5
AGGGGGT	13125	0.0	43.21371	6
AGGGATC	5545	0.0	43.09648	6
GTAGGGC	17970	0.0	43.056206	4
AGGGGAG	27370	0.0	42.781513	6
AGGGATA	2780	0.0	42.69065	6
AGGGGGG	27460	0.0	42.683178	6
GTAGGGT	11095	0.0	42.600273	4
>>END_MODULE