FastQCFastQC Report
Fri 17 Jun 2016
SRR1527562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3014863
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG204350.677808577039819No Hit
GTGGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT190470.6317700008259082No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC174140.5776050188681874No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT133990.4444314716788126No Hit
GTGGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC106290.3525533332692066No Hit
GTGGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG88330.29298180381662453No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA68970.22876661393900818No Hit
GTGGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC63230.2097276061963678No Hit
GTGGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT61090.2026294395466726No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC60660.20120317241612637No Hit
GTGGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC58570.19427085078161097No Hit
GTGGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG57760.1915841615356983No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT56020.18581275500744146No Hit
GTGGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA53190.1764259271482651No Hit
GTGGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT49010.1625612838792343No Hit
GTGGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA48780.16179839680940725No Hit
GTGGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG43730.14504805027624804No Hit
GTGGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG42180.1399068548056744No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41200.13665629250815045No Hit
GTGGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA39600.13134925202239703No Hit
GTGGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC38850.12886157679470012No Hit
GTGGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG37200.12338869129376691No Hit
GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG35860.11894404488694843No Hit
GTGGTAGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG35650.1182474958231933No Hit
GTGGTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT31700.10514573962398954No Hit
GTGGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG31080.10308926143576011No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT31070.10305609243272415No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC31070.10305609243272415No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT30980.10275757140540051No Hit
GTGGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC30920.10255855738718475No Hit
GTGGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC30760.1020278533386094No Hit
GTGGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA30580.10143081128396216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA206.313092E-446.00000431
CCGAATA253.4184857E-546.039
TGGTAGG3088300.044.1865432
TAGGGGG723900.044.173095
TAGGGGA584800.044.159375
GTAGGGA724450.044.14594
GTAGGGG2018550.044.121084
GGTAGGG3046700.044.0930823
TAGGGAT212900.044.0446245
GTGGTAG3135000.044.0169371
AGGGATA30400.043.805926
TAGGGAG281350.043.7110375
TAGGGGT293900.043.652265
TAGGGGC422300.043.6417245
AGGGGGG279600.043.359446
GTAGGGC192500.042.989094
AGGGGAG287200.042.9408076
GTAGGGT117650.042.6765864
TAGGGAA117500.042.6527675
AGGGATT79000.042.5354426
Produced by FastQC (version 0.11.5)