##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527562_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3014863
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.92437367800792	31.0	30.0	31.0	27.0	33.0
2	29.625962440084344	31.0	30.0	31.0	25.0	33.0
3	30.822678178079734	31.0	31.0	33.0	30.0	34.0
4	35.679251760361915	37.0	35.0	37.0	35.0	37.0
5	33.21561444085519	35.0	33.0	35.0	28.0	35.0
6	34.194669210508074	35.0	35.0	35.0	32.0	37.0
7	35.51409433861505	35.0	35.0	37.0	35.0	37.0
8	36.14176597742584	37.0	35.0	37.0	35.0	37.0
9	38.4379973484699	39.0	39.0	39.0	37.0	39.0
10	36.883547610621115	39.0	37.0	39.0	32.0	39.0
11	36.34429756841356	37.0	35.0	39.0	31.0	39.0
12	36.15816705435703	37.0	35.0	39.0	32.0	39.0
13	36.104403417336044	37.0	35.0	39.0	32.0	39.0
14	36.81275268561125	38.0	36.0	40.0	32.0	40.0
15	36.91877110170512	38.0	36.0	40.0	32.0	40.0
16	36.93734275819498	38.0	36.0	40.0	32.0	40.0
17	36.80335856057141	38.0	36.0	40.0	32.0	40.0
18	36.675044935706865	38.0	35.0	40.0	31.0	40.0
19	36.5507746123124	38.0	35.0	40.0	31.0	40.0
20	36.37202950847186	38.0	35.0	40.0	30.0	40.0
21	36.38124286244516	38.0	35.0	40.0	30.0	40.0
22	36.404410747685716	38.0	35.0	40.0	31.0	40.0
23	36.344081306513765	38.0	35.0	40.0	31.0	40.0
24	36.30288706319325	38.0	35.0	40.0	31.0	40.0
25	36.26354796221254	38.0	35.0	40.0	31.0	40.0
26	36.11953810173132	38.0	35.0	40.0	30.0	40.0
27	36.01964832232841	38.0	35.0	40.0	30.0	40.0
28	35.73296066852789	38.0	34.0	40.0	30.0	40.0
29	35.68958788508798	38.0	34.0	40.0	30.0	40.0
30	35.552417141342744	38.0	34.0	40.0	30.0	40.0
31	35.41540759895226	38.0	34.0	39.0	29.0	40.0
32	35.32173800268868	38.0	34.0	39.0	29.0	40.0
33	35.10927561219199	37.0	33.0	39.0	28.0	40.0
34	34.618418482033846	37.0	33.0	39.0	26.0	40.0
35	34.45083010405448	37.0	33.0	39.0	26.0	40.0
36	34.46323332104975	37.0	33.0	39.0	26.0	40.0
37	34.356598624879474	36.0	33.0	39.0	26.0	40.0
38	34.296794580715606	36.0	33.0	39.0	26.0	40.0
39	34.134429989024376	36.0	32.0	39.0	26.0	40.0
40	34.67221760988808	37.0	33.0	39.0	27.0	40.0
41	34.9282803231855	37.0	33.0	39.0	28.0	40.0
42	35.055984301774245	37.0	33.0	40.0	28.0	40.0
43	35.05391521936486	37.0	33.0	40.0	28.0	40.0
44	34.925374055139486	36.0	33.0	40.0	28.0	40.0
45	34.793293426600144	36.0	33.0	39.0	28.0	40.0
46	34.44766843468509	36.0	33.0	39.0	26.0	40.0
47	34.356148521508274	36.0	33.0	39.0	26.0	40.0
48	34.19353317215409	35.0	33.0	39.0	26.0	40.0
49	33.98189668983301	35.0	33.0	39.0	26.0	40.0
50	33.863891991112034	35.0	32.0	39.0	26.0	40.0
51	33.70506852218492	35.0	32.0	38.0	26.0	40.0
52	33.110197047096335	35.0	31.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	17.0
12	54.0
13	87.0
14	157.0
15	241.0
16	413.0
17	740.0
18	1170.0
19	1898.0
20	3070.0
21	4727.0
22	6988.0
23	9962.0
24	14234.0
25	19839.0
26	26569.0
27	36074.0
28	48151.0
29	64270.0
30	85158.0
31	115362.0
32	158773.0
33	228217.0
34	289328.0
35	355908.0
36	504927.0
37	626272.0
38	406816.0
39	5435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.49762228001737	0.39563986821291713	0.07181089157285091	0.03492696019686467
8	98.72591225538275	1.063166054311589	0.12362087431501863	0.0873008159906437
9	98.28012085457947	1.0323188814881472	0.3286053130772443	0.35895495085514667
10	66.29677700114401	23.52047174282878	3.932848689973641	6.249902566053582
11	35.98107111334744	25.46782391106992	19.383534177174884	19.167570798407755
12	30.709455122836427	17.185092655951532	27.18747087346921	24.91798134774283
13	24.21420144132586	16.940570765570442	30.26137506082366	28.58385273228004
14	25.82611548186435	16.48250683364385	31.014808964785463	26.676568719706335
15	26.2310095019243	16.35281603177325	28.792087733339788	28.62408673296266
16	31.36062899043837	17.02322792113605	25.808635417264398	25.80750767116118
17	32.24773397663509	17.412930537805533	23.047216407511716	27.292119078047662
18	30.709189770812138	19.05237485086387	23.433104588832062	26.805330789491926
19	32.06202072863676	18.87681131779454	21.690571014337966	27.37059693923074
20	30.304030398727903	20.32082386496501	22.523842708607322	26.851303027699768
21	32.321136980353664	18.616335136953154	24.34230013105073	24.720227751642447
22	32.8235147003363	17.543450564752032	23.88612683229719	25.74690790261448
23	32.47613573154071	19.05983787654696	22.956797705235694	25.50722868667664
24	32.53962120335153	18.587776625339195	22.214972952336474	26.6576292189728
25	30.061067451489503	18.61507471483779	22.675358714475582	28.648499119197123
26	29.212471677817533	18.264013986705198	22.810190711816755	29.71332362366051
27	28.438605667985577	18.057570111809394	22.857556048152105	30.646268172052928
28	31.117168508154435	19.751577434861883	21.097774592079308	28.033479464904374
29	31.29249985820251	20.47151064575737	21.006593002733457	27.229396493306663
30	31.090401122704414	18.713487146845477	22.470042585682997	27.726069144767106
31	32.88507636997104	20.033248608643245	20.461792127867835	26.619882893517882
32	35.48277318073823	19.761428628763564	19.834035576409274	24.921762614088934
33	34.887256900230625	18.97127663844095	21.85336448123845	24.288101980089973
34	31.285003663516385	21.306905156221028	23.902644995809098	23.505446184453486
35	30.383636006014203	23.387795730684942	23.887752113445952	22.340816149854902
36	31.49065148233933	22.994378185675433	20.74359597766134	24.771374354323893
37	31.54889625167047	24.009780875615245	19.82614135368672	24.615181519027566
38	32.640687155602095	22.530377002205405	20.676660929534773	24.152274912657724
39	32.40707786721983	21.420873850652583	20.092587955074574	26.079460327053006
40	29.783708248102812	21.31798360323504	21.17747970637472	27.720828442287427
41	27.51955893186523	21.967432682679114	22.05582807576994	28.457180309685715
42	27.14501454958318	19.80415030467388	22.199914224958146	30.85092092078479
43	27.337129415167453	18.613681616710277	25.05161926097471	28.99756970714756
44	27.83257481351557	18.48030905550269	24.22600960640666	29.461106524575083
45	28.930501983008845	18.82387358894915	24.052104523489128	28.19351990455288
46	28.88021777440633	20.783033922271095	23.047647604551187	27.289100698771385
47	27.410864108916392	20.635465027764113	23.931468859447342	28.02220200387215
48	29.164774651451825	20.164664198671716	24.448540447774906	26.222020702101556
49	29.21947033745812	19.770914963631846	24.26362325584944	26.745991443060596
50	26.881685834480706	20.486801556156948	23.68100971752282	28.95050289183953
51	26.10493412138462	21.2311471532869	23.571153979467724	29.092764745860755
52	27.812275383657564	20.34254956195356	24.205179472500078	27.639995581888794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	5.0
6	10.0
7	13.0
8	16.0
9	16.0
10	16.0
11	271.5
12	527.0
13	960.0
14	1837.5
15	2282.0
16	2232.5
17	2183.0
18	2462.5
19	2742.0
20	2730.5
21	2719.0
22	2984.0
23	3249.0
24	3614.0
25	3979.0
26	4847.0
27	5715.0
28	7039.5
29	8364.0
30	10130.0
31	11896.0
32	12923.5
33	13951.0
34	17205.5
35	20460.0
36	23916.0
37	27372.0
38	29495.5
39	37386.5
40	43154.0
41	44920.5
42	46687.0
43	51485.0
44	56283.0
45	61748.5
46	67214.0
47	78073.5
48	88933.0
49	95071.0
50	101209.0
51	110227.0
52	119245.0
53	135417.0
54	151589.0
55	169615.0
56	187641.0
57	214790.5
58	241940.0
59	280923.0
60	319906.0
61	310315.0
62	300724.0
63	305147.0
64	271072.0
65	232574.0
66	209439.0
67	186304.0
68	154977.0
69	123650.0
70	111577.5
71	99505.0
72	86370.5
73	73236.0
74	72939.0
75	72642.0
76	53631.0
77	34620.0
78	23480.5
79	12341.0
80	8341.5
81	4342.0
82	3089.0
83	1836.0
84	1248.5
85	661.0
86	474.0
87	287.0
88	214.0
89	118.5
90	96.0
91	65.0
92	34.0
93	20.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3014863.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.404446087507468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.8475363875732	7.16320480602214
2	9.730237559288565	2.0247546420851625
3	3.508071351355634	1.094986177389275
4	1.7266028263404638	0.718573840847895
5	0.9641843115632511	0.5015901844040176
6	0.6810477247611908	0.42515546011784616
7	0.5331008293494645	0.3882633186720415
8	0.43688353017809656	0.36364249090063583
9	0.3798139194607551	0.3556578103482897
>10	9.218283327051168	25.69476209507229
>50	2.566858624210064	18.199134584336626
>100	1.2944437139681488	25.66127587522475
>500	0.07038497699149439	5.006716119006578
>1k	0.038071873918126505	7.818446082814614
>5k	0.0028793854223793155	1.8901093987728972
>10k+	0.0015996585679885088	2.69372711398503
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	20435	0.677808577039819	No Hit
GTGGTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	19047	0.6317700008259082	No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	17414	0.5776050188681874	No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	13399	0.4444314716788126	No Hit
GTGGTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	10629	0.3525533332692066	No Hit
GTGGTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	8833	0.29298180381662453	No Hit
GTGGTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6897	0.22876661393900818	No Hit
GTGGTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	6323	0.2097276061963678	No Hit
GTGGTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	6109	0.2026294395466726	No Hit
GTGGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	6066	0.20120317241612637	No Hit
GTGGTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5857	0.19427085078161097	No Hit
GTGGTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	5776	0.1915841615356983	No Hit
GTGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5602	0.18581275500744146	No Hit
GTGGTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	5319	0.1764259271482651	No Hit
GTGGTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4901	0.1625612838792343	No Hit
GTGGTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4878	0.16179839680940725	No Hit
GTGGTAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	4373	0.14504805027624804	No Hit
GTGGTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	4218	0.1399068548056744	No Hit
GTGGTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4120	0.13665629250815045	No Hit
GTGGTAGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	3960	0.13134925202239703	No Hit
GTGGTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3885	0.12886157679470012	No Hit
GTGGTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3720	0.12338869129376691	No Hit
GTGGTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3586	0.11894404488694843	No Hit
GTGGTAGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3565	0.1182474958231933	No Hit
GTGGTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	3170	0.10514573962398954	No Hit
GTGGTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3108	0.10308926143576011	No Hit
GTGGTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3107	0.10305609243272415	No Hit
GTGGTAGGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3107	0.10305609243272415	No Hit
GTGGTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3098	0.10275757140540051	No Hit
GTGGTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	3092	0.10255855738718475	No Hit
GTGGTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3076	0.1020278533386094	No Hit
GTGGTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	3058	0.10143081128396216	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.032737805996491384	0.0	0.0	0.0	0.0
7	0.03379921409364207	0.0	0.0	0.0	0.0
8	0.03389872110274994	0.0	0.0	0.0	0.0
9	0.03419724213007357	0.0	0.0	0.0	0.0
10	0.04059885971601363	0.0	0.0	0.0	0.0
11	0.05744871325828072	0.0	0.0	0.0	0.0
12	0.06451371090493996	0.0	0.0	0.0	0.0
13	0.07041779344534063	0.0	0.0	0.0	0.0
14	0.07831201616789885	0.0	0.0	0.0	0.0
15	0.08517799979634232	0.0	0.0	0.0	0.0
16	0.09151327937621045	0.0	0.0	0.0	0.0
17	0.09658813684071217	0.0	0.0	0.0	0.0
18	0.10189517732646558	0.0	0.0	0.0	0.0
19	0.10803144288811797	0.0	0.0	0.0	0.0
20	0.11413453944673439	0.0	0.0	0.0	0.0
21	0.1192093969112361	0.0	0.0	0.0	0.0
22	0.12398573334841417	0.0	0.0	0.0	0.0
23	0.12925960483113164	0.0	0.0	0.0	0.0
24	0.1350973493654604	0.0	0.0	0.0	0.0
25	0.14057023486639358	0.0	0.0	0.0	0.0
26	0.1461426273764347	0.0	0.0	0.0	0.0
27	0.153141287017022	0.0	0.0	0.0	0.0
28	0.1641865650279963	0.0	0.0	0.0	0.0
29	0.1699911405592891	0.0	0.0	0.0	0.0
30	0.17874775736078224	0.0	0.0	0.0	0.0
31	0.18544789597404593	0.0	0.0	0.0	0.0
32	0.1940054987573233	0.0	0.0	0.0	0.0
33	0.19917986323093287	0.0	0.0	0.0	0.0
34	0.20737260698081472	0.0	0.0	0.0	0.0
35	0.22150260227413318	0.0	0.0	0.0	0.0
36	0.23115478215759722	0.0	0.0	0.0	0.0
37	0.2384187938224722	0.0	0.0	0.0	0.0
38	0.24561646748127527	0.0	0.0	0.0	0.0
39	0.2525819581188266	0.0	0.0	0.0	0.0
40	0.2596137867624499	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTATA	20	6.313092E-4	46.000004	31
CCGAATA	25	3.4184857E-5	46.0	39
TGGTAGG	308830	0.0	44.186543	2
TAGGGGG	72390	0.0	44.17309	5
TAGGGGA	58480	0.0	44.15937	5
GTAGGGA	72445	0.0	44.1459	4
GTAGGGG	201855	0.0	44.12108	4
GGTAGGG	304670	0.0	44.093082	3
TAGGGAT	21290	0.0	44.044624	5
GTGGTAG	313500	0.0	44.016937	1
AGGGATA	3040	0.0	43.80592	6
TAGGGAG	28135	0.0	43.711037	5
TAGGGGT	29390	0.0	43.65226	5
TAGGGGC	42230	0.0	43.641724	5
AGGGGGG	27960	0.0	43.35944	6
GTAGGGC	19250	0.0	42.98909	4
AGGGGAG	28720	0.0	42.940807	6
GTAGGGT	11765	0.0	42.676586	4
TAGGGAA	11750	0.0	42.652767	5
AGGGATT	7900	0.0	42.535442	6
>>END_MODULE