FastQCFastQC Report
Fri 17 Jun 2016
SRR1527561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences962649
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC76950.7993567748992623No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC70200.7292377595572218No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG50910.5288531957130792No Hit
ATACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA35630.37012452098324516No Hit
ATACAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA33740.3504911966874738No Hit
ATACAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG31650.3287802719371235No Hit
ATACAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA24820.2578302164132513No Hit
ATACAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA23220.24120941277661953No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC21680.22521188927636138No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT21020.21835580777625074No Hit
ATACAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG20190.209733765889748No Hit
ATACAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA20010.2078639254806269No Hit
ATACAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC19740.20505916486694528No Hit
ATACAGGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCT17020.17680379868467114No Hit
ATACAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA15400.15997523500258143No Hit
ATACAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG14760.15332691354792868No Hit
ATACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC13790.14325055134322065No Hit
ATACAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC13770.14304279129776273No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG12870.13369358925215735No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACT12730.13223926893395205No Hit
ATACAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA12340.128187948047523No Hit
ATACAGGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTC11930.12392886711563612No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCAACTGC11560.12008530627466502No Hit
ATACAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC11200.11634562545642284No Hit
ATACAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT11180.11613786541096495No Hit
ATACAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT10720.1113593843654333No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT10440.10845074372902272No Hit
ATACAGGGGATTCACCTGAATGAATGACGCCACCTGGACATTCTCTGCTT10340.10741194350173323No Hit
ATACAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA10290.10689254338808848No Hit
ATACAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT10290.10689254338808848No Hit
ATACAGGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA9880.10263346245620159No Hit
ATACAGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA9740.1011791421379963No Hit
ATACAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA9700.10076362204708052No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA207.855844E-444.020
CGTATTG302.5272857E-644.036
CGTATAG207.855844E-444.032
CAACGAA254.4423003E-544.038
CGCATAT254.4423003E-544.043
ATTACGC551.8189894E-1244.010
TTATCGC207.855844E-444.024
TCGATAA408.310963E-944.014
CGTTATT351.445751E-744.032
AACGGTA302.5272857E-644.044
CACCTAG254.4423003E-544.011
TACGACG207.855844E-444.022
CGTACTG351.445751E-744.027
TTACGCG207.855844E-444.035
ACGTACT351.445751E-744.026
ACGTAAC254.4423003E-544.029
ATAGTAC302.5272857E-644.035
ACATACG207.855844E-444.017
ACGAACG254.4423003E-544.040
TAATCGT302.5272857E-644.021
Produced by FastQC (version 0.11.5)