FastQCFastQC Report
Fri 17 Jun 2016
SRR1527560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832611
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA75710.909308188337651No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC52620.6319878070311347No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT42030.5047975585237283No Hit
ATACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA31830.38229137015965436No Hit
ATACAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG29810.35803034069931816No Hit
ATACAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA27410.329205355201889No Hit
ATACAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG22370.26867288565728775No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC19930.2393674837349014No Hit
ATACAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC19830.23816644267250853No Hit
ATACAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG18810.22591582383610112No Hit
ATACAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG18220.2188296815679831No Hit
ATACAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT16440.19745115065738983No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15920.19120573713294683No Hit
ATACAGGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGA14370.17258960066585716No Hit
ATACAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG13860.16646429124765347No Hit
ATACAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC13120.15757658738594613No Hit
ATACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC12610.15145127796774244No Hit
ATACAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC11970.14376461516842798No Hit
ATACAGGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG11230.13487691130672066No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG11010.13223462096945632No Hit
ATACAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG10970.13175420454449915No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCAACTGCTC10770.12935212241971342No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGC9910.11902316928313462No Hit
ATACAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG9240.11097619416510231No Hit
ATACAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT9220.11073598595262374No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA9210.11061588184638445No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT9050.10869421614655583No Hit
ATACAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA8940.10737307097792366No Hit
ATACAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC8860.10641223812800937No Hit
ATACAGGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA8850.10629213402177007No Hit
ATACAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA8820.10593182170305221No Hit
ATACAGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT8790.10557150938433435No Hit
ATACAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG8650.10389005189698432No Hit
ATACAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA8510.10220859440963426No Hit
ATACAGGGGGAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACT8440.10136786566595925No Hit
ATACAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC8340.10016682460356637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG351.019107E-746.00000432
CGGTAAT351.019107E-746.00000445
ATACCCG351.019107E-746.00000438
AACGTAC206.310447E-446.025
ACCGGTA405.6079443E-946.024
TCCTCGA206.310447E-446.030
TACGGTT206.310447E-446.045
CAACACG253.4163393E-546.018
TAACGAC206.310447E-446.014
GTAATCA253.4163393E-546.040
GTAATAC206.310447E-446.038
CCGTATT253.4163393E-546.018
ACCTATA206.310447E-446.044
GGTATAA253.4163393E-546.020
AATTCGA206.310447E-446.023
ACGTCGT501.6370905E-1146.037
ATACGCG253.4163393E-546.014
CCTATAC206.310447E-446.045
TCGGTTA206.310447E-446.019
AATACGC253.4163393E-546.013
Produced by FastQC (version 0.11.5)