##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527560_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	832611
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.793602294468844	33.0	31.0	34.0	30.0	34.0
2	32.275393911442436	33.0	31.0	34.0	31.0	34.0
3	32.84098576646237	34.0	31.0	34.0	31.0	34.0
4	36.531150801514755	37.0	37.0	37.0	35.0	37.0
5	35.88549394615252	37.0	35.0	37.0	35.0	37.0
6	36.32923538122845	37.0	37.0	37.0	35.0	37.0
7	36.65070843407065	37.0	37.0	37.0	35.0	37.0
8	36.69990908119158	37.0	37.0	37.0	35.0	37.0
9	38.60017222928835	39.0	39.0	39.0	39.0	39.0
10	37.613889319261936	39.0	37.0	39.0	35.0	39.0
11	36.66000929605782	37.0	35.0	39.0	34.0	39.0
12	36.57682519207649	39.0	35.0	39.0	33.0	39.0
13	36.745292819816214	39.0	35.0	39.0	33.0	39.0
14	37.411906640676136	40.0	35.0	40.0	33.0	40.0
15	37.58605038847673	40.0	35.0	40.0	33.0	40.0
16	37.754355875673035	40.0	35.0	40.0	34.0	40.0
17	37.68060234611361	40.0	35.0	40.0	34.0	40.0
18	37.576848011856676	40.0	35.0	40.0	34.0	40.0
19	37.466209310230106	40.0	35.0	40.0	33.0	40.0
20	37.3164142678874	39.0	35.0	40.0	33.0	40.0
21	37.31530570698682	39.0	35.0	40.0	33.0	40.0
22	37.345782123945035	39.0	35.0	40.0	33.0	40.0
23	37.24587832733413	39.0	35.0	40.0	33.0	40.0
24	37.21179158094236	39.0	35.0	40.0	33.0	40.0
25	37.205148622826265	39.0	35.0	40.0	33.0	40.0
26	37.14885102406766	39.0	35.0	40.0	33.0	40.0
27	37.09249337325594	39.0	35.0	40.0	33.0	40.0
28	36.997554680396966	39.0	35.0	40.0	33.0	40.0
29	36.93049935684251	39.0	35.0	40.0	33.0	40.0
30	36.790820683368345	39.0	35.0	40.0	33.0	40.0
31	36.74114922815096	39.0	35.0	40.0	32.0	40.0
32	36.68761042071267	38.0	35.0	40.0	32.0	40.0
33	36.46232754551645	38.0	35.0	40.0	32.0	40.0
34	36.00635590930219	38.0	35.0	40.0	30.0	40.0
35	35.92079374401732	38.0	35.0	40.0	30.0	40.0
36	35.80912695124134	38.0	34.0	40.0	30.0	40.0
37	35.64810938121163	38.0	34.0	40.0	30.0	40.0
38	35.75387666028914	38.0	34.0	40.0	30.0	40.0
39	35.72330416004593	37.0	34.0	40.0	30.0	40.0
40	35.56655508995197	37.0	34.0	40.0	30.0	40.0
41	35.39356314053021	37.0	34.0	40.0	30.0	40.0
42	35.32636969725358	37.0	34.0	40.0	30.0	40.0
43	35.324829962611595	36.0	34.0	40.0	30.0	40.0
44	35.11585722504267	36.0	34.0	40.0	29.0	40.0
45	35.00452191959991	36.0	34.0	40.0	29.0	40.0
46	34.89125173700564	35.0	34.0	40.0	29.0	40.0
47	34.712399908240464	35.0	34.0	39.0	28.0	40.0
48	34.51113305012785	35.0	33.0	39.0	28.0	40.0
49	34.40742675751341	35.0	33.0	39.0	27.0	40.0
50	34.81853951004731	35.0	34.0	39.0	29.0	40.0
51	34.95605510856811	35.0	34.0	39.0	30.0	40.0
52	34.61185115257906	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	19.0
15	40.0
16	72.0
17	133.0
18	226.0
19	379.0
20	605.0
21	954.0
22	1383.0
23	1923.0
24	2626.0
25	3596.0
26	4766.0
27	6367.0
28	8096.0
29	10584.0
30	14298.0
31	19617.0
32	27617.0
33	47184.0
34	103122.0
35	61764.0
36	77677.0
37	138147.0
38	249495.0
39	51910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.93083324625785	0.9414960888097803	0.09884567943493419	0.028824985497429168
8	98.99412811024597	0.8015748050410096	0.14712753014312807	0.05716955456990119
9	95.93099298471915	2.1080672727119865	1.2761061287924373	0.6848336137764214
10	61.75320768041739	27.03111056663916	4.888717540363988	6.326964212579464
11	36.305789858649476	24.590354919644348	19.89752717655664	19.206328045149533
12	31.096994875157787	16.92951450317135	27.387339345744895	24.58615127592597
13	25.609798573403424	17.61398780462905	29.338310447495893	27.437903174471636
14	23.742179721382495	17.40284478586038	32.11968133978533	26.735294152971793
15	26.130209665738263	18.9151956916255	29.747625241559383	25.20696940107685
16	32.38919495418629	19.77610192514872	23.660869241458496	24.173833879206498
17	31.071773012847537	20.243907418950748	22.808610503584507	25.875709064617208
18	30.44855280557187	19.996012543672855	23.96569346309381	25.589741187661463
19	30.302626316491132	21.645762546975718	22.4655931761651	25.586017960368046
20	29.0259196671675	22.100236484985185	24.19869542919803	24.675148418649286
21	29.79242407318664	20.516063323688975	25.239157301548982	24.452355301575405
22	29.368336474055713	19.57192494454193	25.370310985562284	25.689427595840076
23	28.738030124511926	20.343834035341835	25.43804970148124	25.480086138664994
24	28.756526156872773	21.108056463342425	23.52310983160203	26.612307548182766
25	29.38575156946041	20.04741710114327	21.601203923560945	28.965627405835377
26	26.80231224425332	20.608183173174506	24.87704342123753	27.712461161334645
27	26.649780029329424	19.67809697445746	24.802098458944215	28.870024537268907
28	27.057653574118046	21.358593628957582	22.046429845389984	29.53732295153439
29	29.782095120050062	21.224437342288297	21.215669742532828	27.777797795128816
30	28.43152444538926	19.70980445850463	23.679485377925584	28.17918571818052
31	30.319440891364636	20.686491050442523	22.333478659301882	26.66058939889096
32	33.835969017944755	19.854409802416733	21.838289429277296	24.471331750361212
33	34.47312130154418	19.60723555177628	21.62042058055923	24.299222566120314
34	29.114196185253377	21.10757604691747	26.417738896075114	23.360488871754036
35	28.704641182977404	23.910325470117495	25.96194381289702	21.423089534008078
36	29.704027450994523	25.05071395885954	21.742566456604585	23.502692133541352
37	31.283636656253638	24.39110220739337	20.7955455789078	23.529715557445193
38	30.89822257933176	24.178878251668547	21.546436451115827	23.37646271788386
39	30.705935905242665	21.818111939429098	21.251580870298376	26.224371285029864
40	30.181921689720646	20.966934138511263	21.969443113290602	26.881701058477486
41	26.4650599139334	22.71552982124906	21.971845195415384	28.847565069402158
42	27.245256188063816	20.52651238093179	24.175635440800086	28.05259599020431
43	27.45255587543283	18.40715532223331	24.48562413900369	29.654664663330177
44	26.563305072837135	19.727699970334285	25.584096294668218	28.12489866216036
45	28.452782872193616	19.310938721683957	23.801270941652223	28.435007464470203
46	27.024264632583524	22.22238236103054	22.82650601541416	27.926846990971775
47	27.04396170600677	21.84177244835824	24.442026348438826	26.67223949719617
48	27.454237332920172	21.406635271453293	25.210212211945315	25.928915183681216
49	29.062551419570482	21.22936161064411	23.98226782975483	25.725819140030577
50	25.874868335873536	23.0968603585588	23.043414031282317	27.984857274285353
51	27.6114536079874	22.909978369250467	24.175635440800086	25.302932581962047
52	27.134760410323665	23.00233842694848	23.13697513004272	26.72592603268513
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.5
6	3.0
7	29.0
8	55.0
9	108.5
10	162.0
11	230.5
12	299.0
13	605.0
14	753.5
15	596.0
16	749.0
17	902.0
18	851.5
19	801.0
20	919.5
21	1038.0
22	1389.0
23	1740.0
24	2468.0
25	3196.0
26	3401.0
27	3606.0
28	3892.0
29	4178.0
30	6004.0
31	7830.0
32	7341.0
33	6852.0
34	8158.0
35	9464.0
36	11054.0
37	12644.0
38	13492.0
39	16447.5
40	18555.0
41	19973.0
42	21391.0
43	23635.5
44	25880.0
45	27114.0
46	28348.0
47	32831.0
48	37314.0
49	38591.5
50	39869.0
51	44684.5
52	49500.0
53	52952.5
54	56405.0
55	64224.5
56	72044.0
57	70539.0
58	69034.0
59	70624.0
60	72214.0
61	73080.5
62	73947.0
63	64695.5
64	47788.5
65	40133.0
66	35103.5
67	30074.0
68	29299.5
69	28525.0
70	23766.5
71	19008.0
72	16080.5
73	13153.0
74	10133.0
75	7113.0
76	5342.5
77	3572.0
78	2357.0
79	1142.0
80	982.0
81	822.0
82	532.0
83	242.0
84	226.5
85	211.0
86	127.0
87	43.0
88	23.5
89	4.5
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	832611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.323908764116736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.72212010516763	6.888330805141897
2	9.63028455757463	1.9884435828976557
3	3.49240326671165	1.0816575807910296
4	1.5682077293561973	0.6476013408422421
5	0.8957863142464926	0.4624008090212597
6	0.610763396077154	0.37832793465375786
7	0.5502687358942739	0.3976646957582833
8	0.4269526978291724	0.3526256559185502
9	0.39670536773773235	0.36859950204837555
>10	11.64405872635473	32.08473104486969
>50	2.6699085599944157	18.686997889770854
>100	1.3041252704809325	25.998935877618717
>500	0.06282137788222154	4.648389223779172
>1k	0.02326717699341539	4.473998061519725
>5k	0.002326717699341539	1.5412959953687857
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7571	0.909308188337651	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5262	0.6319878070311347	No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4203	0.5047975585237283	No Hit
ATACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3183	0.38229137015965436	No Hit
ATACAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	2981	0.35803034069931816	No Hit
ATACAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2741	0.329205355201889	No Hit
ATACAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	2237	0.26867288565728775	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1993	0.2393674837349014	No Hit
ATACAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1983	0.23816644267250853	No Hit
ATACAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1881	0.22591582383610112	No Hit
ATACAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1822	0.2188296815679831	No Hit
ATACAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1644	0.19745115065738983	No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1592	0.19120573713294683	No Hit
ATACAGGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGA	1437	0.17258960066585716	No Hit
ATACAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	1386	0.16646429124765347	No Hit
ATACAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1312	0.15757658738594613	No Hit
ATACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1261	0.15145127796774244	No Hit
ATACAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1197	0.14376461516842798	No Hit
ATACAGGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG	1123	0.13487691130672066	No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1101	0.13223462096945632	No Hit
ATACAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1097	0.13175420454449915	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCAACTGCTC	1077	0.12935212241971342	No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGC	991	0.11902316928313462	No Hit
ATACAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	924	0.11097619416510231	No Hit
ATACAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	922	0.11073598595262374	No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	921	0.11061588184638445	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	905	0.10869421614655583	No Hit
ATACAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	894	0.10737307097792366	No Hit
ATACAGGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAAC	886	0.10641223812800937	No Hit
ATACAGGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	885	0.10629213402177007	No Hit
ATACAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	882	0.10593182170305221	No Hit
ATACAGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT	879	0.10557150938433435	No Hit
ATACAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	865	0.10389005189698432	No Hit
ATACAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	851	0.10220859440963426	No Hit
ATACAGGGGGAGTGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACT	844	0.10136786566595925	No Hit
ATACAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACC	834	0.10016682460356637	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.05212518210785109	0.0	0.0	0.0	0.0
6	0.05212518210785109	0.0	0.0	0.0	0.0
7	0.05260559853280824	0.0	0.0	0.0	0.0
8	0.053446327276483255	0.0	0.0	0.0	0.0
9	0.05392674370144041	0.0	0.0	0.0	0.0
10	0.06497632147545493	0.0	0.0	0.0	0.0
11	0.08239141688015171	0.0	0.0	0.0	0.0
12	0.09163943306057691	0.0	0.0	0.0	0.0
13	0.10112765745348068	0.0	0.0	0.0	0.0
14	0.11073598595262374	0.0	0.0	0.0	0.0
15	0.11830254464569889	0.0	0.0	0.0	0.0
16	0.1263495197637312	0.0	0.0	0.0	0.0
17	0.13331555792560992	0.0	0.0	0.0	0.0
18	0.14100222072492435	0.0	0.0	0.0	0.0
19	0.15169148618022102	0.0	0.0	0.0	0.0
20	0.16045908593568906	0.0	0.0	0.0	0.0
21	0.16814574873500351	0.0	0.0	0.0	0.0
22	0.17511178689688223	0.0	0.0	0.0	0.0
23	0.18363917843987168	0.0	0.0	0.0	0.0
24	0.19048511249551112	0.0	0.0	0.0	0.0
25	0.19733104655115052	0.0	0.0	0.0	0.0
26	0.20513781345670426	0.0	0.0	0.0	0.0
27	0.2100620818125151	0.0	0.0	0.0	0.0
28	0.22051113905533315	0.0	0.0	0.0	0.0
29	0.22759728132345117	0.0	0.0	0.0	0.0
30	0.23600456876020134	0.0	0.0	0.0	0.0
31	0.24237008639088362	0.0	0.0	0.0	0.0
32	0.25089747793387307	0.0	0.0	0.0	0.0
33	0.2564222668208803	0.0	0.0	0.0	0.0
34	0.2638687214077162	0.0	0.0	0.0	0.0
35	0.2731167375881414	0.0	0.0	0.0	0.0
36	0.28632818927446313	0.0	0.0	0.0	0.0
37	0.2932942274363418	0.0	0.0	0.0	0.0
38	0.30350307646668134	0.0	0.0	0.0	0.0
39	0.3121505721159101	0.0	0.0	0.0	0.0
40	0.3210382759776174	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCG	35	1.019107E-7	46.000004	32
CGGTAAT	35	1.019107E-7	46.000004	45
ATACCCG	35	1.019107E-7	46.000004	38
AACGTAC	20	6.310447E-4	46.0	25
ACCGGTA	40	5.6079443E-9	46.0	24
TCCTCGA	20	6.310447E-4	46.0	30
TACGGTT	20	6.310447E-4	46.0	45
CAACACG	25	3.4163393E-5	46.0	18
TAACGAC	20	6.310447E-4	46.0	14
GTAATCA	25	3.4163393E-5	46.0	40
GTAATAC	20	6.310447E-4	46.0	38
CCGTATT	25	3.4163393E-5	46.0	18
ACCTATA	20	6.310447E-4	46.0	44
GGTATAA	25	3.4163393E-5	46.0	20
AATTCGA	20	6.310447E-4	46.0	23
ACGTCGT	50	1.6370905E-11	46.0	37
ATACGCG	25	3.4163393E-5	46.0	14
CCTATAC	20	6.310447E-4	46.0	45
TCGGTTA	20	6.310447E-4	46.0	19
AATACGC	25	3.4163393E-5	46.0	13
>>END_MODULE