SRR1527559_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527559_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1088747
Sequences flagged as poor quality	0
Sequence length	52
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9109	0.8366498369226276	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6513	0.598210603565383	No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5425	0.4982792145466302	No Hit
ATACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4241	0.3895303500262228	No Hit
ATACAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	3940	0.36188389038040975	No Hit
ATACAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	3663	0.3364417996100104	No Hit
ATACAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	2980	0.2737091353638632	No Hit
ATACAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2742	0.25184914401601105	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2438	0.22392713826077135	No Hit
ATACAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2305	0.21171126074285393	No Hit
ATACAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2305	0.21171126074285393	No Hit
ATACAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2248	0.2064758846637465	No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2017	0.18525883423788997	No Hit
ATACAGGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGA	1905	0.17497177948595954	No Hit
ATACAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	1860	0.17083858784455894	No Hit
ATACAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1704	0.15651019015437012	No Hit
ATACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1682	0.15448951868524094	No Hit
ATACAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1598	0.1467742276212931	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCAACTGCTC	1470	0.13501759361908688	No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1449	0.13308877085309995	No Hit
ATACAGGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG	1425	0.13088440197768628	No Hit
ATACAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1399	0.12849633569598815	No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGC	1276	0.11719894520949312	No Hit
ATACAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1231	0.1130657535680925	No Hit
ATACAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	1231	0.1130657535680925	No Hit
ATACAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	1209	0.11104508209896331	No Hit
ATACAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1194	0.10966735155182976	No Hit
ATACAGGGGATTCACCTGAATGAATGACGCCACCTGGACATTCTCTGCTTTG	1185	0.10884071322354964	No Hit
ATACAGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT	1183	0.10865701581726517	No Hit
ATACAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1170	0.10746298267641609	No Hit
ATACAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1122	0.10305424492558876	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	1108	0.10176836308159747	No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1107	0.10167651437845523	No Hit
ATACAGGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	1096	0.10066617864389064	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	20	6.311308E-4	46.0	11
TAGACGC	20	6.311308E-4	46.0	17
TAACGAA	20	6.311308E-4	46.0	30
CGTTATT	40	5.6115823E-9	46.0	32
AACGCAC	25	3.4170356E-5	46.0	35
TCGTCTA	25	3.4170356E-5	46.0	36
CGTAATT	20	6.311308E-4	46.0	40
CGTAACG	20	6.311308E-4	46.0	28
GCGTGTA	25	3.4170356E-5	46.0	16
CGCGTTA	25	3.4170356E-5	46.0	27
CGCAATT	20	6.311308E-4	46.0	21
TATACGT	20	6.311308E-4	46.0	22
ACATACG	20	6.311308E-4	46.0	17
CGTCATA	25	3.4170356E-5	46.0	35
GATTACG	95	0.0	46.0	9
TACCGAA	20	6.311308E-4	46.0	24
TCGATAA	35	1.0195072E-7	45.999996	14
ACGATAA	35	1.0195072E-7	45.999996	38
CTATTAG	35	1.0195072E-7	45.999996	25
GACGATA	35	1.0195072E-7	45.999996	37

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content