##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527559_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1088747
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.18574379539048	31.0	30.0	33.0	27.0	34.0
2	30.84775158967143	31.0	31.0	33.0	28.0	34.0
3	31.78732340938712	33.0	31.0	34.0	30.0	34.0
4	36.0975855731405	37.0	35.0	37.0	35.0	37.0
5	35.15224565486748	35.0	35.0	37.0	33.0	37.0
6	35.82768815895704	37.0	35.0	37.0	35.0	37.0
7	36.44825841081537	37.0	37.0	37.0	35.0	37.0
8	36.54180998891386	37.0	37.0	37.0	35.0	37.0
9	38.49116828794936	39.0	39.0	39.0	37.0	39.0
10	37.12741160251188	39.0	37.0	39.0	34.0	39.0
11	36.02648089960294	37.0	35.0	39.0	32.0	39.0
12	35.92472998777494	38.0	35.0	39.0	31.0	39.0
13	36.064492485398354	38.0	35.0	39.0	32.0	39.0
14	36.78074979770323	39.0	35.0	40.0	32.0	40.0
15	36.9852913486788	39.0	35.0	40.0	33.0	40.0
16	37.11790985417181	39.0	35.0	40.0	33.0	40.0
17	37.07369756242727	39.0	35.0	40.0	33.0	40.0
18	36.99574005714826	39.0	35.0	40.0	33.0	40.0
19	36.8569144162969	39.0	35.0	40.0	32.0	40.0
20	36.64138500496442	39.0	35.0	40.0	31.0	40.0
21	36.70283270585361	39.0	35.0	40.0	31.0	40.0
22	36.76437225544594	39.0	35.0	40.0	32.0	40.0
23	36.72394137480976	38.0	35.0	40.0	32.0	40.0
24	36.73148031636367	38.0	35.0	40.0	32.0	40.0
25	36.70018562622905	38.0	35.0	40.0	32.0	40.0
26	36.59623401947376	38.0	35.0	40.0	32.0	40.0
27	36.536196196177805	38.0	35.0	40.0	32.0	40.0
28	36.37693513736433	38.0	35.0	40.0	31.0	40.0
29	36.324580458086224	38.0	35.0	40.0	31.0	40.0
30	36.243321910416284	38.0	35.0	40.0	31.0	40.0
31	36.178596129311956	38.0	35.0	40.0	31.0	40.0
32	36.139976505101735	38.0	35.0	40.0	31.0	40.0
33	35.95725545053167	38.0	35.0	40.0	31.0	40.0
34	35.52073163002975	38.0	34.0	40.0	29.0	40.0
35	35.45191215222636	37.0	34.0	40.0	29.0	40.0
36	35.3936571122584	37.0	34.0	40.0	29.0	40.0
37	35.31098133909898	37.0	34.0	40.0	29.0	40.0
38	35.179825983447024	37.0	34.0	40.0	29.0	40.0
39	35.06174804614846	36.0	34.0	40.0	29.0	40.0
40	35.354940128422854	37.0	34.0	40.0	30.0	40.0
41	35.39982934510956	37.0	34.0	40.0	30.0	40.0
42	35.488807776278605	37.0	34.0	40.0	30.0	40.0
43	35.47364906631201	36.0	34.0	40.0	30.0	40.0
44	35.31178501525148	36.0	34.0	40.0	30.0	40.0
45	35.21563228188	36.0	34.0	40.0	30.0	40.0
46	34.99624522501554	35.0	34.0	40.0	30.0	40.0
47	34.90874372099303	35.0	34.0	39.0	29.0	40.0
48	34.80099325187578	35.0	34.0	39.0	29.0	40.0
49	34.701092172929066	35.0	34.0	39.0	29.0	40.0
50	34.57785693094906	35.0	34.0	39.0	29.0	40.0
51	34.45694178721043	35.0	34.0	39.0	29.0	40.0
52	33.987654615810655	35.0	33.0	38.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	8.0
14	11.0
15	31.0
16	78.0
17	184.0
18	356.0
19	577.0
20	955.0
21	1528.0
22	2249.0
23	3206.0
24	4380.0
25	6149.0
26	8144.0
27	10901.0
28	13909.0
29	18232.0
30	24307.0
31	32511.0
32	46304.0
33	73140.0
34	134611.0
35	84439.0
36	118630.0
37	193132.0
38	278326.0
39	32445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.96697763575926	0.9079244305610027	0.09745147403391238	0.02764645964581303
8	99.02181131153519	0.7806221280058636	0.14071221321390553	0.056854347245044075
9	95.91879472457788	2.143381336527219	1.269257228722559	0.6685667101723357
10	61.715439858846906	27.062944834750407	4.751608959657294	6.470006346745387
11	35.915047297489686	24.547025158278277	20.090434233113847	19.447493311118194
12	30.7391891780184	16.997153608689622	27.512084993115938	24.751572220176037
13	25.380368441887786	17.43839477858492	29.595856521303848	27.585380258223445
14	23.46183273065276	17.508016095566738	32.0800883951919	26.950062778588595
15	25.97490509732748	18.93635527813165	29.777074012603478	25.31166561193739
16	32.263602104070095	19.82315450696994	23.6943936470089	24.218849741951068
17	30.943001450291025	20.24914879214363	22.7101429441367	26.09770681342865
18	30.351587650758166	20.079136842627353	23.99501445239344	25.574261054221044
19	30.177350660897346	21.575444065517516	22.53177276263448	25.715432510950663
20	28.813672965344566	22.102380075444525	24.289481394667447	24.79446556454346
21	29.723801764780983	20.55647455285755	25.304501413092297	24.41522226926917
22	29.51135571441299	19.584807122315837	25.146521643687652	25.757315519583525
23	28.44480857352535	20.423845025520162	25.51823334530428	25.61311305565021
24	28.5997573357263	21.126395755855125	23.475058943905243	26.798787964513338
25	29.177669375897246	20.16161697804908	21.670415624566587	28.99029802148709
26	26.744964624471983	20.752847080175652	24.732192143813027	27.76999615153934
27	26.54969428159159	19.774842089117122	24.715016436325428	28.96044719296586
28	27.13761783040504	21.341964662129953	22.042402872292644	29.478014635172357
29	29.672274642318186	21.35215986817874	21.11206735816494	27.863498131338133
30	28.2383326888616	19.75050218278443	23.64102955048326	28.37013557787071
31	30.2381545023775	20.757990607551616	22.30472276846687	26.69913212160401
32	33.73988630967525	19.883315407528105	21.79073742568292	24.58606085711373
33	34.1610126135824	19.766483857131178	21.616041192306383	24.456462336980035
34	28.97927617711002	21.282538551196925	26.232540709641448	23.505644562051607
35	28.53243223632304	23.90169617000093	26.02744255552484	21.538429038151197
36	29.57445577347171	25.0122388396937	21.73994509284526	23.67336029398933
37	31.054322078499414	24.48998711362695	20.707290123417103	23.748400684456534
38	30.830486788941787	24.294533073340272	21.411310433002342	23.463669704715603
39	30.45252937551148	21.800932631731705	21.389634139060774	26.35690385369604
40	30.040955336731123	21.025362182398666	21.973791891045394	26.959890589824813
41	26.388867202389537	22.857468263976845	21.896271585593347	28.85739294804027
42	27.022806951477246	20.725476166639265	24.039285527307996	28.21243135457549
43	27.40948999170606	18.39573381143645	24.430561002693924	29.76421519416357
44	26.481358846453766	19.802580397466077	25.472400842436304	28.24365991364385
45	28.368390452511004	19.386230226122322	23.646632321374938	28.598746999991732
46	27.144047239625003	22.211680032183786	22.891635981545758	27.752636746645454
47	26.983954950048084	21.961484164824334	24.48677240901697	26.56778847611061
48	27.311211879343873	21.39734943012472	25.23809480072046	26.053343889810947
49	28.893856883187734	21.260403013739648	24.026794103680654	25.818945999391964
50	25.7942386982467	23.106286400789163	23.04043088063618	28.059044020327956
51	27.682280640038503	22.8325772654253	24.130766835637665	25.354375258898532
52	27.017663424101286	23.09425422067753	23.071843137110825	26.81623921811036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	3.0
6	2.0
7	37.0
8	72.0
9	128.5
10	185.0
11	285.5
12	386.0
13	657.0
14	840.5
15	753.0
16	991.0
17	1229.0
18	1141.0
19	1053.0
20	1242.5
21	1432.0
22	1946.0
23	2460.0
24	3400.5
25	4341.0
26	4530.0
27	4719.0
28	5187.5
29	5656.0
30	7828.5
31	10001.0
32	9442.5
33	8884.0
34	10587.5
35	12291.0
36	14142.5
37	15994.0
38	17537.0
39	21866.0
40	24652.0
41	26299.5
42	27947.0
43	30961.0
44	33975.0
45	35556.5
46	37138.0
47	43391.5
48	49645.0
49	51362.0
50	53079.0
51	58829.0
52	64579.0
53	69423.0
54	74267.0
55	83791.0
56	93315.0
57	91766.0
58	90217.0
59	92007.5
60	93798.0
61	94845.5
62	95893.0
63	84156.0
64	62757.0
65	53095.0
66	45898.0
67	38701.0
68	38137.0
69	37573.0
70	31252.0
71	24931.0
72	21331.5
73	17732.0
74	13364.0
75	8996.0
76	6644.5
77	4293.0
78	2893.5
79	1494.0
80	1283.5
81	1073.0
82	710.5
83	348.0
84	218.5
85	89.0
86	53.5
87	18.0
88	13.0
89	4.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1088747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.671923426784053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.34222282377308	6.610007459677466
2	9.851337533009758	1.905627645413482
3	3.881103724854161	1.1261321411458742
4	1.7584921986263051	0.6803200756684302
5	0.9751207505436423	0.4715646615563151
6	0.5896288341935131	0.3421706960726166
7	0.39308583376810163	0.26613272590510506
8	0.33231893814860086	0.2571330658434763
9	0.2886427346304235	0.25125573843387056
>10	8.55578842631485	23.58424477989053
>50	3.3782593741897307	22.426594985112924
>100	1.5277176315546028	28.607247383798708
>500	0.0845039584887257	5.305848976106095
>1k	0.03892878986559274	6.232905510951985
>5k	0.00284844803894581	1.9328141544231263
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9109	0.8366498369226276	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	6513	0.598210603565383	No Hit
ATACAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5425	0.4982792145466302	No Hit
ATACAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4241	0.3895303500262228	No Hit
ATACAGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	3940	0.36188389038040975	No Hit
ATACAGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	3663	0.3364417996100104	No Hit
ATACAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	2980	0.2737091353638632	No Hit
ATACAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2742	0.25184914401601105	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2438	0.22392713826077135	No Hit
ATACAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2305	0.21171126074285393	No Hit
ATACAGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2305	0.21171126074285393	No Hit
ATACAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2248	0.2064758846637465	No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2017	0.18525883423788997	No Hit
ATACAGGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGA	1905	0.17497177948595954	No Hit
ATACAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	1860	0.17083858784455894	No Hit
ATACAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1704	0.15651019015437012	No Hit
ATACAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1682	0.15448951868524094	No Hit
ATACAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1598	0.1467742276212931	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGATAAGAGCACCCAACTGCTC	1470	0.13501759361908688	No Hit
ATACAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1449	0.13308877085309995	No Hit
ATACAGGGGGGGGCGGACCGCGCTGCTGGAGGTGTGAGGAGCTTAGACTCGG	1425	0.13088440197768628	No Hit
ATACAGGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG	1399	0.12849633569598815	No Hit
ATACAGGGGGGGGGCTGGTGAGATGGCTCAGTTGGTAAGAGCACCCGACTGC	1276	0.11719894520949312	No Hit
ATACAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1231	0.1130657535680925	No Hit
ATACAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA	1231	0.1130657535680925	No Hit
ATACAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	1209	0.11104508209896331	No Hit
ATACAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1194	0.10966735155182976	No Hit
ATACAGGGGATTCACCTGAATGAATGACGCCACCTGGACATTCTCTGCTTTG	1185	0.10884071322354964	No Hit
ATACAGGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT	1183	0.10865701581726517	No Hit
ATACAGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1170	0.10746298267641609	No Hit
ATACAGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	1122	0.10305424492558876	No Hit
ATACAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTT	1108	0.10176836308159747	No Hit
ATACAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1107	0.10167651437845523	No Hit
ATACAGGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA	1096	0.10066617864389064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0405971267888683	0.0	0.0	0.0	0.0
6	0.0405971267888683	0.0	0.0	0.0	0.0
7	0.04114821900772172	0.0	0.0	0.0	0.0
8	0.041699311226575136	0.0	0.0	0.0	0.0
9	0.0426177982579975	0.0	0.0	0.0	0.0
10	0.054098886150776994	0.0	0.0	0.0	0.0
11	0.06879467865353475	0.0	0.0	0.0	0.0
12	0.07871433859289624	0.0	0.0	0.0	0.0
13	0.0857866887348484	0.0	0.0	0.0	0.0
14	0.09414492072079188	0.0	0.0	0.0	0.0
15	0.10241130400359312	0.0	0.0	0.0	0.0
16	0.11095323339582107	0.0	0.0	0.0	0.0
17	0.1182092809440577	0.0	0.0	0.0	0.0
18	0.124546841460872	0.0	0.0	0.0	0.0
19	0.13455835010337572	0.0	0.0	0.0	0.0
20	0.14254918727675026	0.0	0.0	0.0	0.0
21	0.14971338612184465	0.0	0.0	0.0	0.0
22	0.15660203885751234	0.0	0.0	0.0	0.0
23	0.16321514548375335	0.0	0.0	0.0	0.0
24	0.1712059826571279	0.0	0.0	0.0	0.0
25	0.17864572761164899	0.0	0.0	0.0	0.0
26	0.18645286737873903	0.0	0.0	0.0	0.0
27	0.19260673048926885	0.0	0.0	0.0	0.0
28	0.20197529820977692	0.0	0.0	0.0	0.0
29	0.20895579964858685	0.0	0.0	0.0	0.0
30	0.21611999849368127	0.0	0.0	0.0	0.0
31	0.22190646679164214	0.0	0.0	0.0	0.0
32	0.2272336915738918	0.0	0.0	0.0	0.0
33	0.23246906765299927	0.0	0.0	0.0	0.0
34	0.23981696390437815	0.0	0.0	0.0	0.0
35	0.25028771606259304	0.0	0.0	0.0	0.0
36	0.26130956043966136	0.0	0.0	0.0	0.0
37	0.27086182556645394	0.0	0.0	0.0	0.0
38	0.27931190625553964	0.0	0.0	0.0	0.0
39	0.2891397174917589	0.0	0.0	0.0	0.0
40	0.29630391633685327	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	20	6.311308E-4	46.0	11
TAGACGC	20	6.311308E-4	46.0	17
TAACGAA	20	6.311308E-4	46.0	30
CGTTATT	40	5.6115823E-9	46.0	32
AACGCAC	25	3.4170356E-5	46.0	35
TCGTCTA	25	3.4170356E-5	46.0	36
CGTAATT	20	6.311308E-4	46.0	40
CGTAACG	20	6.311308E-4	46.0	28
GCGTGTA	25	3.4170356E-5	46.0	16
CGCGTTA	25	3.4170356E-5	46.0	27
CGCAATT	20	6.311308E-4	46.0	21
TATACGT	20	6.311308E-4	46.0	22
ACATACG	20	6.311308E-4	46.0	17
CGTCATA	25	3.4170356E-5	46.0	35
GATTACG	95	0.0	46.0	9
TACCGAA	20	6.311308E-4	46.0	24
TCGATAA	35	1.0195072E-7	45.999996	14
ACGATAA	35	1.0195072E-7	45.999996	38
CTATTAG	35	1.0195072E-7	45.999996	25
GACGATA	35	1.0195072E-7	45.999996	37
>>END_MODULE