FastQCFastQC Report
Fri 17 Jun 2016
SRR1527558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092611
Sequences flagged as poor quality0
Sequence length50
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA395111.8881196744163153No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA329931.5766427682928168No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAA244801.1698304175979195No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAA220401.0532296733602184No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA148920.7116468373720678No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA146210.6986965088112411No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA126720.6055592749918642No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA122160.5837683162326873No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79660.38067275762193736No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77450.3701117885741784No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA74430.3556800571152498No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA68280.32629093510451773No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAA67900.3244750218745863No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAA67110.32069983384393946No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA59510.28438156924531127Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA58790.2809408915464938No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAA53110.25379776747804533No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAA48210.23038204424998243No Hit
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA45260.21628482312288333No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA43040.20567606688486295Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAA36240.173180777507143No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA32160.153683603880511Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAA29760.1422146782177863No Hit
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA27560.13170149636028866Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGA25320.1209971657417456No Hit
TAAGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA24180.11554942605195137No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA23830.11387687439280401Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC22860.10924151693745279No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCA302.5287172E-644.027
GATCGTT302.5287172E-644.09
CGGCATC254.4440963E-544.037
TACGGAC600.044.013
ATCGTTC551.8189894E-1244.022
CGTTAAC254.4440963E-544.012
CTACGTT207.857965E-444.031
ACCGTAG254.4440963E-544.034
ATCGGGT207.857965E-444.020
CACCGTA254.4440963E-544.033
CGCAACC207.857965E-444.027
CAATCGT207.857965E-444.011
CTAGGCG207.857965E-444.028
ATCGATT207.857965E-444.029
CTAATAT207.857965E-444.022
TGCAACG254.4440963E-544.013
CCCGATA207.857965E-444.043
TTGCGAC1350.040.7407413
CATTAGC603.6379788E-1240.33333232
CGCTAAT603.6379788E-1240.33333244
Produced by FastQC (version 0.11.5)