##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527558_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2092611
Sequences flagged as poor quality	0
Sequence length	50
%GC	42
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.060163116795238	31.0	31.0	33.0	28.0	33.0
2	29.05371089036615	31.0	28.0	31.0	25.0	33.0
3	31.23556552077763	31.0	31.0	33.0	30.0	33.0
4	35.58376210389795	35.0	35.0	37.0	35.0	37.0
5	35.981740036729235	37.0	35.0	37.0	35.0	37.0
6	36.2265088924793	37.0	37.0	37.0	35.0	37.0
7	36.47610138721435	37.0	37.0	37.0	35.0	37.0
8	36.345719295177176	37.0	37.0	37.0	35.0	37.0
9	33.629120749150225	35.0	33.0	35.0	30.0	35.0
10	30.37636187518846	34.0	26.0	35.0	20.0	37.0
11	30.898313159970964	33.0	26.0	35.0	21.0	39.0
12	32.79133149926097	35.0	32.0	35.0	24.0	39.0
13	33.25772396302992	35.0	33.0	35.0	27.0	39.0
14	33.95574380522706	35.0	33.0	35.0	29.0	40.0
15	34.06813593161844	35.0	34.0	35.0	29.0	40.0
16	34.25036855870489	35.0	34.0	35.0	30.0	40.0
17	34.323967521914014	35.0	34.0	35.0	31.0	40.0
18	34.157794735858694	35.0	34.0	35.0	30.0	40.0
19	34.105496434836674	35.0	34.0	35.0	30.0	40.0
20	34.040335733683904	35.0	33.0	35.0	29.0	40.0
21	33.94038595802086	35.0	33.0	36.0	29.0	39.0
22	33.5900604555744	35.0	33.0	35.0	29.0	37.0
23	32.96316706736226	35.0	33.0	35.0	27.0	36.0
24	32.7171194264008	34.0	33.0	35.0	27.0	35.0
25	32.54253322762807	34.0	33.0	35.0	25.0	35.0
26	31.837283183544386	34.0	32.0	35.0	23.0	35.0
27	31.671943328215324	34.0	31.0	35.0	22.0	35.0
28	31.304287801220582	34.0	31.0	35.0	21.0	35.0
29	30.801575161365395	33.0	30.0	35.0	21.0	35.0
30	30.033565244567672	33.0	27.0	35.0	18.0	35.0
31	29.934319374217186	33.0	27.0	35.0	18.0	35.0
32	29.789876857189416	33.0	27.0	35.0	18.0	35.0
33	29.310204333246837	33.0	25.0	35.0	15.0	35.0
34	28.751594539071046	33.0	24.0	35.0	15.0	35.0
35	28.131903158303192	33.0	23.0	35.0	10.0	35.0
36	27.29350509961001	32.0	21.0	35.0	9.0	35.0
37	26.41973830778869	32.0	18.0	34.0	8.0	35.0
38	25.570273691574783	31.0	17.0	34.0	8.0	35.0
39	24.64053519741605	30.0	15.0	34.0	8.0	35.0
40	24.314440667663508	29.0	15.0	34.0	8.0	35.0
41	23.994959885043134	29.0	12.0	34.0	8.0	35.0
42	23.805955335224752	29.0	12.0	33.0	8.0	35.0
43	23.647769222277816	27.0	10.0	33.0	8.0	35.0
44	23.378340742737183	27.0	10.0	33.0	8.0	35.0
45	23.226370787499445	25.0	10.0	33.0	8.0	35.0
46	23.10061019463245	25.0	10.0	33.0	8.0	35.0
47	22.88224615086129	24.0	10.0	33.0	8.0	35.0
48	22.465253217153116	23.0	10.0	33.0	8.0	35.0
49	21.643092289966937	23.0	10.0	31.0	8.0	35.0
50	21.773410347169158	24.0	9.0	32.0	8.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	15.0
11	57.0
12	100.0
13	200.0
14	492.0
15	1349.0
16	3331.0
17	6491.0
18	10938.0
19	15810.0
20	21642.0
21	30380.0
22	41190.0
23	55115.0
24	74204.0
25	98995.0
26	128534.0
27	148444.0
28	143495.0
29	128762.0
30	140613.0
31	203358.0
32	296930.0
33	284923.0
34	147512.0
35	54710.0
36	46074.0
37	8820.0
38	124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.59319290589603	1.0482120183827763	0.19798232925278517	0.16061274646840717
8	94.31279869980612	4.450612177800843	0.6862718393432893	0.5503172830497403
9	76.95730357911718	18.434434302409763	2.7524465846734056	1.855815533799641
10	30.785129199836952	50.452855308511715	6.399756094180906	12.362259397470433
11	19.37880475635462	60.07447155730329	10.3124278712097	10.234295815132386
12	31.606925510761435	45.499808612303006	13.487456579364249	9.405809297571311
13	19.51442480231634	45.84984978096741	21.517711605262516	13.11801381145373
14	26.959669045035128	46.315822673205865	14.413811262580575	12.310697019178434
15	19.402746138675557	46.04969581064039	15.478892159125609	19.06866589155844
16	22.215882454980882	50.40626279800689	15.290132757593264	12.087721989418961
17	26.5899873411733	45.23640561958243	13.211533342795198	14.962073696449076
18	21.271559788226288	48.181721304150656	18.435198897453947	12.111520010169114
19	26.00550221708669	44.94351793047059	15.735127073306984	13.315852779135732
20	19.58113571992119	42.044555820455884	19.519490244484043	18.854818215138884
21	19.981210076789235	42.004701303777914	24.312975512410095	13.701113107022758
22	16.27086926332701	45.779937121615056	21.659926283480303	16.28926733157763
23	16.980604613088623	53.39090734016021	18.194972692010126	11.433515354741038
24	20.275818104750478	53.240951137120085	16.650060618050848	9.833170140078591
25	28.92625528586058	49.36316400898208	10.815722559042268	10.89485814611507
26	31.327848319635137	47.404414867359485	10.268415868978993	10.999320944026387
27	35.49250195091204	42.14242398611113	10.673268944873175	11.691805118103652
28	36.41751859280105	42.45695927241135	9.865330919124482	11.260191215663113
29	34.90366819251165	43.91150576958642	10.217474724160391	10.967351313741542
30	29.1811999459049	48.77743641794868	10.234486963893433	11.806876672252988
31	24.965175085097037	53.01353189866631	10.987374146461049	11.033918869775604
32	21.611231136603983	55.99750742015597	11.696392688368741	10.694868754871306
33	19.635183032106777	57.39838890266753	11.690514863966595	11.275913201259097
34	17.82667681666588	58.31915248462328	12.27241947977909	11.581751218931755
35	19.671740232656713	57.14635926122915	11.945316162440129	11.236584343674004
36	21.06468904158489	54.5130461418773	12.346776347825754	12.075488468712054
37	22.72978589905147	51.9719145125396	12.78398135152687	12.514318236882058
38	23.895124320764825	49.81991397350009	12.438145455605461	13.846816250129624
39	24.950791140828372	47.25895066020392	13.19332642330562	14.596931775662082
40	25.095060668227397	47.148944548222296	13.54465784610709	14.211336937443223
41	24.597643804796974	47.29144594958164	13.752102039031621	14.358808206589757
42	23.9754068004039	47.83751017269813	13.584894660307148	14.602188366590829
43	23.544079621104927	47.793593744847946	13.770882404804333	14.891444229242797
44	23.664312191802487	47.24026586881174	13.669525774260002	15.425896165125769
45	23.725957667239637	46.762537327768996	14.017464306552915	15.494040698438457
46	23.39479243872846	46.37225934490453	14.113803282119802	16.119144934247213
47	22.24942906254435	46.13791096386285	14.85087290471091	16.76178706888189
48	21.56588109304596	45.612920891651626	15.607344126548126	17.213853888754286
49	19.932371568342134	46.09676619304782	15.877532900285814	18.093329338324228
50	20.102016093769937	42.9340665799807	18.611963714230694	18.351953612018672
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	156.0
6	312.0
7	1409.0
8	2506.0
9	6199.5
10	9893.0
11	11266.0
12	12639.0
13	9829.0
14	7019.0
15	7391.5
16	7764.0
17	10107.0
18	12450.0
19	16488.5
20	20527.0
21	27384.0
22	34241.0
23	44594.5
24	54948.0
25	66784.5
26	78621.0
27	88625.5
28	98630.0
29	102735.5
30	106841.0
31	107420.0
32	107999.0
33	116326.0
34	124653.0
35	148381.0
36	172109.0
37	174461.0
38	176813.0
39	147954.0
40	119095.0
41	100572.5
42	82050.0
43	77642.5
44	73235.0
45	74719.5
46	76204.0
47	71760.0
48	67316.0
49	62703.0
50	58090.0
51	61753.5
52	65417.0
53	70804.0
54	76191.0
55	80335.5
56	84480.0
57	85607.5
58	86735.0
59	78160.5
60	69586.0
61	62019.5
62	54453.0
63	47535.5
64	40618.0
65	36527.0
66	32436.0
67	27953.0
68	23470.0
69	21126.5
70	18783.0
71	16269.5
72	13756.0
73	11028.0
74	8300.0
75	8832.5
76	9365.0
77	6605.0
78	3845.0
79	2298.5
80	752.0
81	571.5
82	391.0
83	221.0
84	51.0
85	36.5
86	22.0
87	12.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2092611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.846660630080976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.61396871368866	29.48514252330198
2	7.03942791111806	4.906011108973017
3	2.451482597457598	2.5627794634246297
4	1.1795812958756526	1.6441787641188002
5	0.7264777952618664	1.2657662593389853
6	0.4955830545040612	1.0361648708593165
7	0.37199446866746044	0.90739355041456
8	0.27274623206902515	0.7603436309634102
9	0.2438438383523913	0.7647429133621892
>10	2.0355615034751406	15.31769154011777
>50	0.35203537891274156	8.427660109564188
>100	0.19094570426868157	12.788774214130965
>500	0.015038294199018172	3.512520413414636
>1k	0.008967790118680562	5.368563488109555
>5k	0.001241694016432693	2.914592430200092
>10k+	0.0011037280146068383	8.337674719705907
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	39511	1.8881196744163153	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA	32993	1.5766427682928168	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAA	24480	1.1698304175979195	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAA	22040	1.0532296733602184	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA	14892	0.7116468373720678	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA	14621	0.6986965088112411	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA	12672	0.6055592749918642	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	12216	0.5837683162326873	No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7966	0.38067275762193736	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7745	0.3701117885741784	No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	7443	0.3556800571152498	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	6828	0.32629093510451773	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAA	6790	0.3244750218745863	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAA	6711	0.32069983384393946	No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	5951	0.28438156924531127	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA	5879	0.2809408915464938	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAA	5311	0.25379776747804533	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAA	4821	0.23038204424998243	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA	4526	0.21628482312288333	No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	4304	0.20567606688486295	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAA	3624	0.173180777507143	No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	3216	0.153683603880511	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAA	2976	0.1422146782177863	No Hit
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA	2756	0.13170149636028866	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGA	2532	0.1209971657417456	No Hit
TAAGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	2418	0.11554942605195137	No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	2383	0.11387687439280401	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2286	0.10924151693745279	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.02647410340478952	0.0	0.0	0.0	0.0
8	0.08644702718278743	0.0	0.0	0.0	0.0
9	0.20983355243760068	0.0	0.0	0.0	0.0
10	0.5022433696468193	0.0	0.0	0.0	0.0
11	0.868341034239044	0.0	0.0	0.0	0.0
12	1.0771710556811562	0.0	0.0	0.0	0.0
13	1.2202936905139081	0.0	0.0	0.0	0.0
14	1.3871665589065527	0.0	0.0	0.0	0.0
15	1.5495952186048911	0.0	0.0	0.0	0.0
16	1.7315688391201232	0.0	0.0	0.0	0.0
17	1.8890754182215423	0.0	0.0	0.0	0.0
18	2.066365894091162	0.0	0.0	0.0	0.0
19	2.2572279319950055	0.0	0.0	0.0	0.0
20	2.45908102365896	0.0	0.0	0.0	0.0
21	2.6702048302336174	0.0	0.0	0.0	0.0
22	2.9121035873365857	0.0	0.0	0.0	0.0
23	3.1916586503655004	0.0	0.0	0.0	0.0
24	3.4868401246098775	0.0	0.0	0.0	0.0
25	3.754496177263715	0.0	0.0	0.0	4.7787190261352925E-5
26	4.0140284075731225	0.0	0.0	0.0	4.7787190261352925E-5
27	4.258412098569681	0.0	0.0	0.0	4.7787190261352925E-5
28	4.481148192377848	0.0	0.0	0.0	4.7787190261352925E-5
29	4.660971389331318	0.0	0.0	0.0	4.7787190261352925E-5
30	4.85245466070856	0.0	0.0	0.0	4.7787190261352925E-5
31	5.033902622130917	0.0	0.0	0.0	4.7787190261352925E-5
32	5.207131186828321	0.0	0.0	0.0	4.7787190261352925E-5
33	5.384851747410293	0.0	0.0	0.0	4.7787190261352925E-5
34	5.566873155115786	0.0	0.0	0.0	4.7787190261352925E-5
35	5.758165277731981	0.0	0.0	0.0	4.7787190261352925E-5
36	5.923222232894695	0.0	0.0	0.0	4.7787190261352925E-5
37	6.09401365088877	0.0	0.0	0.0	4.7787190261352925E-5
38	6.261937837467165	0.0	0.0	0.0	4.7787190261352925E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	30	2.5287172E-6	44.0	27
GATCGTT	30	2.5287172E-6	44.0	9
CGGCATC	25	4.4440963E-5	44.0	37
TACGGAC	60	0.0	44.0	13
ATCGTTC	55	1.8189894E-12	44.0	22
CGTTAAC	25	4.4440963E-5	44.0	12
CTACGTT	20	7.857965E-4	44.0	31
ACCGTAG	25	4.4440963E-5	44.0	34
ATCGGGT	20	7.857965E-4	44.0	20
CACCGTA	25	4.4440963E-5	44.0	33
CGCAACC	20	7.857965E-4	44.0	27
CAATCGT	20	7.857965E-4	44.0	11
CTAGGCG	20	7.857965E-4	44.0	28
ATCGATT	20	7.857965E-4	44.0	29
CTAATAT	20	7.857965E-4	44.0	22
TGCAACG	25	4.4440963E-5	44.0	13
CCCGATA	20	7.857965E-4	44.0	43
TTGCGAC	135	0.0	40.74074	13
CATTAGC	60	3.6379788E-12	40.333332	32
CGCTAAT	60	3.6379788E-12	40.333332	44
>>END_MODULE