FastQCFastQC Report
Fri 17 Jun 2016
SRR1527557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690154
Sequences flagged as poor quality0
Sequence length52
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA332771.9688738422652612No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA273811.62002989076735No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA206811.223616309519724No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA183511.0857590491754006No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA125780.7441925410347222No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA122780.7264426791878137No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA104340.617340195035482No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA96910.5733797038613049No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA68340.40434185287257846Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65520.38765698273648436No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA62750.37126794363117205No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA60400.35736388518442697No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA57660.34115234469758376No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA52980.31346256021640634No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA50770.3003868286558503Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA48140.28482611643672706No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA46200.2733478724423928No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAA42770.2530538637307606No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAAAA40030.23684232324391744No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA36360.21512832558453254Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA31570.18678771283563508No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA29160.17252865715195181Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAA28720.1699253440810719No Hit
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA28060.166020374474752Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAA23620.13975057894132725No Hit
TAAGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA18600.11004914345083348No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC17820.10543417937063725No Hit
TAAGGGGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA17240.10200253941356824Illumina Single End Adapter 2 (100% over 34bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT206.312301E-446.024
TTAGGGC206.312301E-446.038
TTAGGCT206.312301E-446.028
CGTATAT405.6134013E-946.036
CGAGTAT206.312301E-446.032
TAGACGC253.417844E-546.026
CTATACG206.312301E-446.045
CAACCGT253.417844E-546.012
TTATCGT206.312301E-446.011
GTAATTA206.312301E-446.033
TAACCGT405.6134013E-946.032
TAACCGA206.312301E-446.017
AATGACG206.312301E-446.021
GTAGACG253.417844E-546.025
CTTGCGA405.6134013E-946.033
CGCTAAC206.312301E-446.035
TCGCTAA206.312301E-446.034
TTGCGAC501.6370905E-1146.013
ACGTCGC206.312301E-446.041
ATTATCG206.312301E-446.010
Produced by FastQC (version 0.11.5)