##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527557_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1690154
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40466371703407	31.0	31.0	33.0	30.0	33.0
2	30.025751499567495	31.0	30.0	33.0	25.0	33.0
3	31.642889937839985	31.0	31.0	33.0	30.0	34.0
4	35.95581290225624	37.0	35.0	37.0	35.0	37.0
5	36.16342475301067	37.0	35.0	37.0	35.0	37.0
6	36.32103938457679	37.0	37.0	37.0	35.0	37.0
7	36.52302807909812	37.0	37.0	37.0	35.0	37.0
8	36.400525632575494	37.0	37.0	37.0	35.0	37.0
9	34.050283583626104	35.0	35.0	35.0	31.0	37.0
10	31.17141100751766	35.0	26.0	35.0	21.0	39.0
11	31.434934331427787	33.0	27.0	35.0	21.0	39.0
12	33.19084059795735	35.0	33.0	35.0	25.0	39.0
13	33.50990678955882	35.0	33.0	35.0	27.0	39.0
14	34.23624770287205	35.0	33.0	35.0	30.0	40.0
15	34.43085718816155	35.0	34.0	35.0	31.0	40.0
16	34.60957285549127	35.0	34.0	36.0	31.0	40.0
17	34.73746120176031	35.0	34.0	35.0	31.0	40.0
18	34.57368559314713	35.0	34.0	35.0	31.0	40.0
19	34.575587195013	35.0	34.0	36.0	31.0	40.0
20	34.6719168785803	35.0	34.0	36.0	31.0	40.0
21	34.59721835998376	35.0	34.0	36.0	31.0	39.0
22	34.29070487068042	35.0	34.0	35.0	31.0	38.0
23	33.73965449302253	35.0	34.0	35.0	30.0	37.0
24	33.484319772044444	35.0	34.0	35.0	30.0	36.0
25	33.3834082574724	35.0	33.0	35.0	29.0	35.0
26	33.27973723104522	35.0	34.0	35.0	29.0	35.0
27	33.17909137273882	35.0	33.0	35.0	29.0	35.0
28	32.99412716237692	35.0	33.0	35.0	26.0	35.0
29	32.6627532165708	35.0	33.0	35.0	24.0	35.0
30	31.982433553392177	35.0	33.0	35.0	21.0	35.0
31	31.507606407463463	35.0	32.0	35.0	21.0	35.0
32	31.218727997567086	35.0	32.0	35.0	18.0	35.0
33	30.713227315380728	35.0	31.0	35.0	15.0	35.0
34	30.161465759924834	35.0	30.0	35.0	12.0	35.0
35	29.578863819509937	35.0	29.0	35.0	10.0	35.0
36	28.755746517772938	35.0	23.0	35.0	9.0	35.0
37	27.906596085327134	34.0	21.0	35.0	8.0	35.0
38	27.300779100602668	34.0	18.0	35.0	8.0	35.0
39	26.752760399348226	34.0	15.0	35.0	8.0	35.0
40	26.393729210474312	34.0	14.0	35.0	8.0	35.0
41	26.117367411490314	34.0	10.0	35.0	8.0	35.0
42	25.889609467539643	33.0	10.0	35.0	8.0	35.0
43	25.80009099762507	33.0	10.0	35.0	8.0	35.0
44	25.649259179932717	33.0	10.0	35.0	8.0	35.0
45	25.539486342664635	33.0	10.0	35.0	8.0	35.0
46	25.38470458904928	33.0	10.0	35.0	8.0	35.0
47	25.227273372722248	33.0	10.0	35.0	8.0	35.0
48	24.999406562952252	33.0	9.0	35.0	8.0	35.0
49	24.765887605508137	33.0	9.0	35.0	8.0	35.0
50	24.57852006385217	33.0	9.0	35.0	8.0	35.0
51	24.386312726532612	33.0	9.0	35.0	8.0	35.0
52	23.90670790945677	32.0	9.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	20.0
11	25.0
12	42.0
13	73.0
14	207.0
15	670.0
16	1690.0
17	3401.0
18	5548.0
19	8574.0
20	12939.0
21	20117.0
22	30239.0
23	43971.0
24	63799.0
25	90829.0
26	115973.0
27	109136.0
28	75946.0
29	54057.0
30	58904.0
31	79145.0
32	119647.0
33	250893.0
34	396339.0
35	49649.0
36	37688.0
37	28886.0
38	28586.0
39	3156.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.57941939018575	1.0600809157035394	0.20601672983645278	0.15448296427426142
8	94.2941885768989	4.4659835730945225	0.686150492795331	0.5536773572112363
9	76.60242794443583	18.811244419147606	2.7307570789407354	1.8555705574758279
10	30.346287971391956	50.946600132295636	6.352853053627065	12.354258842685342
11	19.001996267795715	60.6162515368422	10.287997425086708	10.093754770275371
12	31.079002268432344	46.238508443609284	13.417061403872074	9.265427884086302
13	19.27759245607205	46.64728776194359	21.400594265374632	12.67452551660973
14	26.823354558223688	46.875550985294836	14.165277246925429	12.135817209556052
15	18.946853363657986	46.86543356404209	15.225949824690533	18.96176324760939
16	21.976163118863727	51.25497439878259	14.845511119105122	11.923351363248557
17	26.21441596446241	45.94161242111666	13.082772339088628	14.761199275332308
18	20.88744575938051	48.972223832857836	18.242006349717244	11.898324058044416
19	25.924679052914705	45.66832371488042	15.314639967718918	13.092357264485956
20	19.446216143617683	42.50145253036114	19.303566420574693	18.748764905446485
21	19.837837262166644	42.770777100784905	23.913974702896894	13.477410934151562
22	16.165745843278188	46.46836915452675	21.55874553443059	15.807139467764475
23	16.95058556794233	53.8598257910226	17.962268527009964	11.227320114025112
24	20.079117050872284	53.82562772386421	16.44607532804703	9.649179897216467
25	28.74093129975138	50.16749952962866	10.686008493900555	10.4055606767194
26	31.008535316900115	48.46014031857452	10.082158193868725	10.449166170656639
27	35.33743079033035	42.93549581872421	10.487387539833648	11.239685851111792
28	36.18723500935418	43.04246831945491	9.693140388390644	11.077156282800265
29	34.58010335152891	44.61918854731581	10.05476424041833	10.74594386073695
30	29.100188503532813	49.242435896373934	10.06109502447706	11.596280575616188
31	24.71230432256469	53.70540199295449	10.743281381459914	10.839012303020908
32	21.54016734569749	56.7879021674948	11.2671981369745	10.40473234983321
33	19.508577324906486	58.01175514183915	11.498360504427406	10.981307028826958
34	18.326850689345466	58.70210643527158	11.922227205331586	11.048815670051368
35	20.651490929228935	57.09917557808342	11.427361057039773	10.821972435647876
36	22.545697019324866	54.36877349637962	11.601191370727165	11.48433811356835
37	24.82986757419738	51.51820484997225	11.79170655455065	11.860221021279719
38	25.648668701195277	49.655179350520726	11.697277289525095	12.998874658758904
39	27.14125458390182	46.89992746223125	12.459515523437508	13.499302430429417
40	27.511930865471427	45.80653597246168	13.15217429891004	13.529358863156848
41	26.950088571810614	45.99823448040829	13.342689482733528	13.708987465047565
42	26.79607893718561	45.75387804898252	13.527583876972157	13.922459136859718
43	26.409013616510684	45.56572951340529	13.764071202979137	14.261185667104892
44	26.178087913882404	45.29936325328934	13.463506875704818	15.059041957123434
45	26.11797504842754	44.587652959434465	13.85495049563531	15.439421496502687
46	24.658877238405495	44.8083429083977	13.967602952157021	16.565176901039788
47	23.22971752869857	44.283361161172294	14.941774536521525	17.545146773607613
48	22.178984873567735	43.704715664963075	16.23023700798862	17.88606245348057
49	21.02204887838623	43.321496147688315	17.168613037628525	18.487841936296928
50	19.624069759323707	43.18884551348575	18.300225896575107	18.886858830615434
51	19.46367017443381	42.38181846151298	19.20730300315829	18.947208360894923
52	20.0294766039071	40.68487250274235	19.928243225173564	19.357407668176982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	119.0
6	238.0
7	1096.0
8	1954.0
9	4966.0
10	7978.0
11	9010.0
12	10042.0
13	7413.0
14	4840.5
15	4897.0
16	5726.0
17	6555.0
18	7968.5
19	9382.0
20	10983.5
21	12585.0
22	15616.5
23	18648.0
24	23830.0
25	29012.0
26	36897.0
27	44782.0
28	54976.0
29	65170.0
30	77471.0
31	89772.0
32	106300.5
33	122829.0
34	145724.5
35	168620.0
36	167373.5
37	166127.0
38	139932.5
39	97570.0
40	81402.0
41	75199.5
42	68997.0
43	68455.5
44	67914.0
45	64161.5
46	60409.0
47	52245.0
48	44081.0
49	42290.0
50	40499.0
51	42460.0
52	44421.0
53	49991.5
54	55562.0
55	56785.5
56	58009.0
57	58705.0
58	59401.0
59	59859.5
60	60318.0
61	51450.5
62	42583.0
63	38296.5
64	29350.0
65	24690.0
66	22917.5
67	21145.0
68	18315.0
69	15485.0
70	13058.5
71	10632.0
72	9558.5
73	8485.0
74	8520.5
75	8556.0
76	6609.0
77	4662.0
78	2843.0
79	1024.0
80	733.5
81	443.0
82	339.5
83	236.0
84	135.0
85	34.0
86	25.5
87	17.0
88	13.5
89	9.5
90	9.0
91	7.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1690154.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.65950619859722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.10179368892	26.81404039899233
2	7.807465607005164	5.0997594277463945
3	2.7972198063370497	2.7406745281171134
4	1.4144021396291153	1.8477470178610504
5	0.8971902082280763	1.4650894583472793
6	0.6669214235472217	1.3068794619790662
7	0.46372071418204797	1.060142267747257
8	0.3833048656318894	1.0014838108045734
9	0.2970743415594888	0.8732071169645682
>10	2.5827156077236966	18.191269433136565
>50	0.3604417816448244	8.007058483277346
>100	0.20181519619618446	12.30696391015928
>500	0.01680271115756295	3.9082439626166727
>1k	0.006392335766464165	4.252178497932796
>5k	0.001461105318048952	3.0738776289361422
>10k+	0.001278467153292833	8.05138459538161
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	33277	1.9688738422652612	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA	27381	1.62002989076735	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	20681	1.223616309519724	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA	18351	1.0857590491754006	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA	12578	0.7441925410347222	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA	12278	0.7264426791878137	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	10434	0.617340195035482	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	9691	0.5733797038613049	No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	6834	0.40434185287257846	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6552	0.38765698273648436	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6275	0.37126794363117205	No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	6040	0.35736388518442697	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA	5766	0.34115234469758376	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA	5298	0.31346256021640634	No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	5077	0.3003868286558503	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	4814	0.28482611643672706	No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	4620	0.2733478724423928	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAA	4277	0.2530538637307606	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAAAA	4003	0.23684232324391744	No Hit
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3636	0.21512832558453254	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3157	0.18678771283563508	No Hit
TAAGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	2916	0.17252865715195181	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAA	2872	0.1699253440810719	No Hit
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2806	0.166020374474752	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAA	2362	0.13975057894132725	No Hit
TAAGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	1860	0.11004914345083348	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1782	0.10543417937063725	No Hit
TAAGGGGGGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	1724	0.10200253941356824	Illumina Single End Adapter 2 (100% over 34bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.028281446542741076	0.0	0.0	0.0	0.0
8	0.08916347267763766	0.0	0.0	0.0	0.0
9	0.21838246692313246	0.0	0.0	0.0	0.0
10	0.5294783788932843	0.0	0.0	0.0	0.0
11	0.9186736829898341	0.0	0.0	0.0	0.0
12	1.1408427871069737	0.0	0.0	0.0	0.0
13	1.2980473968644277	0.0	0.0	0.0	0.0
14	1.4782084946105503	0.0	0.0	0.0	0.0
15	1.6512696476179094	0.0	0.0	0.0	0.0
16	1.851843086487977	0.0	0.0	0.0	0.0
17	2.0222417602182996	0.0	0.0	0.0	0.0
18	2.2015745310782333	0.0	0.0	0.0	0.0
19	2.388598908738494	0.0	0.0	0.0	0.0
20	2.569588333370805	0.0	0.0	0.0	0.0
21	2.7439511429136045	0.0	0.0	0.0	0.0
22	2.9378979666941594	0.0	0.0	0.0	0.0
23	3.1341522725148123	0.0	0.0	0.0	0.0
24	3.326205777698364	0.0	0.0	0.0	0.0
25	3.4786770909633087	0.0	0.0	0.0	0.0
26	3.6135168747936577	0.0	0.0	0.0	0.0
27	3.739836724937491	0.0	0.0	0.0	0.0
28	3.8707715391615203	0.0	0.0	0.0	0.0
29	3.9683366131133613	0.0	0.0	0.0	0.0
30	4.084184044767518	0.0	0.0	0.0	0.0
31	4.203108119141806	0.0	0.0	0.0	0.0
32	4.325404667267007	0.0	0.0	0.0	0.0
33	4.453972833244781	0.0	0.0	0.0	0.0
34	4.59206675841373	0.0	0.0	0.0	0.0
35	4.739449777949228	0.0	0.0	0.0	0.0
36	4.862101323311367	0.0	0.0	0.0	0.0
37	4.9880070100121054	0.0	0.0	0.0	0.0
38	5.122314297987048	0.0	0.0	0.0	0.0
39	5.272359796799582	0.0	0.0	0.0	0.0
40	5.439918492634399	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	20	6.312301E-4	46.0	24
TTAGGGC	20	6.312301E-4	46.0	38
TTAGGCT	20	6.312301E-4	46.0	28
CGTATAT	40	5.6134013E-9	46.0	36
CGAGTAT	20	6.312301E-4	46.0	32
TAGACGC	25	3.417844E-5	46.0	26
CTATACG	20	6.312301E-4	46.0	45
CAACCGT	25	3.417844E-5	46.0	12
TTATCGT	20	6.312301E-4	46.0	11
GTAATTA	20	6.312301E-4	46.0	33
TAACCGT	40	5.6134013E-9	46.0	32
TAACCGA	20	6.312301E-4	46.0	17
AATGACG	20	6.312301E-4	46.0	21
GTAGACG	25	3.417844E-5	46.0	25
CTTGCGA	40	5.6134013E-9	46.0	33
CGCTAAC	20	6.312301E-4	46.0	35
TCGCTAA	20	6.312301E-4	46.0	34
TTGCGAC	50	1.6370905E-11	46.0	13
ACGTCGC	20	6.312301E-4	46.0	41
ATTATCG	20	6.312301E-4	46.0	10
>>END_MODULE