FastQCFastQC Report
Fri 17 Jun 2016
SRR1527556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207663
Sequences flagged as poor quality0
Sequence length52
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA402561.8234667157079683No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA334281.5141803798858793No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA258791.1722350739220615No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA231691.0494808310869912No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA151420.6858836697448841No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA145460.6588867956748834No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA125330.5677044005357702No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA119840.5428364745887393No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85150.38570198440613446No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA82350.37301888920546294No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA72680.32921691399457254No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA70980.321516463337022No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA68610.3107811291850251No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA57690.2613170579024063No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA56080.2540242781620202No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAA52680.23862337684691912No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA50810.23015288112361354Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAAAA47700.2160655860971534No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAA38820.17584205560359528No Hit
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA37110.16809630817747093No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA35510.16084882520565866Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA30570.13847222153018826Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAA29620.13416902851567472No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG27000.12230127514933212No Hit
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA23430.10613032876847599Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA23420.10608503199990216No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC23400.1059944384627545No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTA351.0201438E-746.00000416
ATCGTTC351.0201438E-746.00000422
TTACGTC351.0201438E-746.00000419
TACGTCG351.0201438E-746.00000440
ATAATAG301.8621886E-646.035
TAGGTCG206.3127256E-446.042
CACATCG253.4181863E-546.012
TACGGAC551.8189894E-1246.013
TTGCGAC1000.046.013
ACGCTAG253.4181863E-546.012
ATAACCG850.046.031
CGGTCGA206.3127256E-446.026
TTACCGT301.8621886E-646.045
CTAGGCG253.4181863E-546.028
CGATAAA253.4181863E-546.024
CCGCATA206.3127256E-446.019
TACGGTC453.110472E-1045.99999618
ATTGCGA1050.043.80952512
CGTATAT900.043.44444336
TAACCGT900.043.44444332
Produced by FastQC (version 0.11.5)