##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527556_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2207663
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.578023004416888	31.0	30.0	33.0	28.0	33.0
2	28.681722708583692	30.0	28.0	31.0	25.0	33.0
3	30.794099914706184	31.0	30.0	33.0	28.0	33.0
4	35.26944918676447	35.0	35.0	37.0	33.0	37.0
5	35.666128843034464	37.0	35.0	37.0	35.0	37.0
6	35.93497286497079	37.0	35.0	37.0	35.0	37.0
7	36.31019861274117	37.0	35.0	37.0	35.0	37.0
8	36.14187219697934	37.0	35.0	37.0	35.0	37.0
9	33.56335047514045	35.0	33.0	35.0	30.0	35.0
10	29.884607387993547	33.0	26.0	35.0	18.0	37.0
11	30.158204852824003	33.0	25.0	35.0	21.0	39.0
12	32.06539856853152	33.0	31.0	35.0	22.0	39.0
13	32.49857337827377	35.0	31.0	35.0	23.0	39.0
14	33.32489741414337	35.0	32.0	35.0	27.0	40.0
15	33.48226472971645	35.0	33.0	35.0	27.0	40.0
16	33.63417333170869	35.0	33.0	35.0	27.0	40.0
17	33.86866111358482	35.0	33.0	35.0	29.0	40.0
18	33.70609418194715	35.0	33.0	35.0	29.0	40.0
19	33.692631982327015	35.0	33.0	35.0	27.0	40.0
20	33.77281677502409	35.0	33.0	35.0	29.0	40.0
21	33.82960352191435	35.0	33.0	36.0	29.0	39.0
22	33.561745157662195	35.0	33.0	35.0	29.0	38.0
23	32.91994475606105	35.0	33.0	35.0	27.0	36.0
24	32.77712404474777	35.0	33.0	35.0	25.0	35.0
25	32.67933103920299	35.0	33.0	35.0	25.0	35.0
26	32.55688164362043	35.0	33.0	35.0	24.0	35.0
27	32.41064963266586	35.0	33.0	35.0	23.0	35.0
28	32.133822055268396	35.0	33.0	35.0	23.0	35.0
29	31.81342442211515	35.0	32.0	35.0	21.0	35.0
30	31.085243535811397	35.0	31.0	35.0	20.0	35.0
31	30.514877950121917	34.0	30.0	35.0	18.0	35.0
32	30.27404273206554	34.0	29.0	35.0	17.0	35.0
33	29.978228561152676	34.0	29.0	35.0	15.0	35.0
34	29.620099172745114	34.0	27.0	35.0	14.0	35.0
35	29.08170721708884	34.0	25.0	35.0	10.0	35.0
36	28.333329860581077	33.0	23.0	35.0	9.0	35.0
37	27.49976694812569	33.0	20.0	35.0	8.0	35.0
38	26.819229655975573	33.0	18.0	35.0	8.0	35.0
39	26.258439807162596	33.0	15.0	35.0	8.0	35.0
40	25.9514405051858	33.0	14.0	35.0	8.0	35.0
41	25.8083602433886	33.0	10.0	35.0	8.0	35.0
42	25.70564257316447	33.0	10.0	35.0	8.0	35.0
43	25.634012528180254	33.0	10.0	35.0	8.0	35.0
44	25.485401530940184	33.0	10.0	35.0	8.0	35.0
45	25.41240488244809	33.0	10.0	35.0	8.0	35.0
46	25.18251970522675	33.0	10.0	35.0	8.0	35.0
47	25.07032776288772	33.0	10.0	35.0	8.0	35.0
48	24.89573318029065	33.0	10.0	35.0	8.0	35.0
49	24.719585371499182	33.0	10.0	35.0	8.0	35.0
50	24.442550334901657	32.0	10.0	35.0	8.0	35.0
51	24.157896381830017	32.0	9.0	35.0	8.0	35.0
52	23.49533692415917	31.0	9.0	35.0	8.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	16.0
11	31.0
12	62.0
13	131.0
14	500.0
15	1663.0
16	4377.0
17	8198.0
18	12939.0
19	17983.0
20	24845.0
21	34625.0
22	48701.0
23	67161.0
24	92643.0
25	124337.0
26	150550.0
27	142721.0
28	108919.0
29	90273.0
30	102684.0
31	131280.0
32	182377.0
33	331142.0
34	376698.0
35	51290.0
36	38717.0
37	30200.0
38	30418.0
39	2181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.59380711639413	1.0464912443611185	0.1990792978819684	0.16062234136278952
8	94.4110582095184	4.35908922693364	0.6813992896560752	0.5484532738918938
9	77.11294703947115	18.372324036775538	2.655568354409165	1.8591605693441438
10	30.8254475433977	50.67041482327692	6.100161120605817	12.40397651271956
11	18.86275215012436	60.27165378049095	10.411462256694069	10.454131812690616
12	30.735533457778658	45.97875672147425	13.654665589811488	9.63104423093561
13	18.949268978100374	46.588904194163696	21.235351591252833	13.226475236483104
14	26.31755843169904	46.96328198642637	14.380772790049932	12.338386791824659
15	18.98759004431383	46.69036895576906	15.552283115674811	18.769757884242296
16	21.917838003354678	50.77921766139125	15.186828786821177	12.11611554843289
17	25.90812094056022	45.77492126289203	13.212614425299513	15.104343371248238
18	20.87619351323096	48.805093893406735	18.088222704280497	12.230489889081802
19	25.535917393189088	45.68727201570167	15.450682463763718	13.326128127345523
20	19.404682689341627	42.79403151658564	19.238534142212828	18.56275165185991
21	19.9384598102156	42.7674423134328	23.678704584893616	13.61539329145798
22	16.260588685863738	46.377232394618204	21.096199918194035	16.265979001324023
23	16.913677495161174	53.612349348609825	17.8632336547743	11.610739501454706
24	19.970937593283033	53.52655726893099	16.485079470915625	10.017425666870352
25	28.17096631143431	50.04608946202387	10.772069831310304	11.010874395231518
26	30.38108624368846	48.4668176256974	10.265561365117774	10.886534765496364
27	34.49108854023463	42.984096757521414	10.755672401086578	11.769142301157377
28	35.404044910840106	43.26317014870476	9.982773638911373	11.35001130154376
29	34.046274272839646	44.62664817954552	10.24517781925955	11.08189972835528
30	28.60232743856286	49.210952939828225	10.190368729285222	11.996350892323694
31	24.770990862282876	53.22809686079805	10.898357222094134	11.102555054824943
32	21.506226267324315	56.16350865145632	11.620115932549488	10.710149148669883
33	19.493373762209178	57.60933620756429	11.579031763453026	11.318258266773507
34	17.922436531300292	58.48007598985896	12.095460221963226	11.502027256877522
35	19.954313679216437	57.1754384613956	11.708761708648467	11.161486150739492
36	21.75282187544023	54.39109139393105	11.940137602523574	11.91594912810515
37	23.746559144217212	51.61358413852114	12.299340977314019	12.340515739947627
38	25.035297506911153	49.402965941812674	11.958165716415957	13.603570834860212
39	26.16880384370259	46.718317061979114	12.697227792466514	14.415651301851778
40	26.114357127876854	46.144089926768714	13.627170451287176	14.114382494067257
41	25.7506693729976	46.19799308137157	13.752280126088085	14.299057419542747
42	25.467564569411184	46.25959668663197	13.688955243621875	14.58388350033497
43	25.414295569568363	45.89255697087825	13.915076712342417	14.778070747210965
44	25.380730664055157	45.42889924775657	13.758712267225567	15.43165782096271
45	25.5237778592113	44.737081701328506	14.048068024875175	15.691072414585017
46	24.53060091146158	44.79234375898858	14.137936813725647	16.5391185158242
47	23.231987853218538	44.40890661301113	14.935159940624995	17.423945593145334
48	22.229932738828346	43.964907687450484	16.0815305596914	17.72362901402977
49	20.969867230641633	43.58042871579585	17.069136004906547	18.380568048655977
50	19.79183417034212	43.20179302728723	17.88384368447539	19.122529117895258
51	19.74599384054541	42.35429048727093	18.52950382372672	19.370211848456943
52	20.305499525969317	40.50640881330167	19.4317248601802	19.756366800548815
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	162.5
6	325.0
7	1479.5
8	2634.0
9	6569.0
10	10504.0
11	11910.5
12	13317.0
13	10322.0
14	7515.0
15	7703.0
16	9073.5
17	10444.0
18	12537.5
19	14631.0
20	18004.5
21	21378.0
22	26832.5
23	32287.0
24	40677.0
25	49067.0
26	59797.0
27	70527.0
28	82531.5
29	94536.0
30	106536.5
31	118537.0
32	135460.5
33	152384.0
34	177499.0
35	202614.0
36	200560.5
37	198507.0
38	166691.0
39	115068.0
40	95261.0
41	88440.5
42	81620.0
43	81749.5
44	81879.0
45	79029.0
46	76179.0
47	67965.0
48	59751.0
49	57965.5
50	56180.0
51	58772.5
52	61365.0
53	68497.5
54	75630.0
55	77012.0
56	78394.0
57	80026.0
58	81658.0
59	81130.5
60	80603.0
61	69348.5
62	58094.0
63	52834.0
64	40943.0
65	34312.0
66	31715.0
67	29118.0
68	25236.5
69	21355.0
70	18180.0
71	15005.0
72	13436.0
73	11867.0
74	11850.5
75	11834.0
76	9013.0
77	6192.0
78	3727.5
79	1263.0
80	922.5
81	582.0
82	414.0
83	246.0
84	146.5
85	47.0
86	36.0
87	25.0
88	21.0
89	14.0
90	11.0
91	7.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2207663.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.700413367494946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.66715788331928	30.226525350854573
2	7.166241987758383	5.116756025089459
3	2.483981467940565	2.6603749560802523
4	1.1640898073932207	1.6623394928330222
5	0.7019058565307915	1.2529164611607433
6	0.4814352336793009	1.031246211121654
7	0.36791780382689876	0.9194372377316841
8	0.27079229423278867	0.77339174726743
9	0.23497925841476333	0.754997099237502
>10	1.8843595251717828	14.517363701740383
>50	0.35882353912712156	8.847121234104826
>100	0.19302217411387845	13.05776086652329
>500	0.015712422850680044	3.8694826290410704
>1k	0.00740910996210929	4.518348823247137
>5k	0.0011496894768790275	2.7227479893649704
>10k+	0.0010219462016702468	8.06919017460211
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	40256	1.8234667157079683	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAA	33428	1.5141803798858793	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAA	25879	1.1722350739220615	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAA	23169	1.0494808310869912	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAA	15142	0.6858836697448841	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAA	14546	0.6588867956748834	No Hit
TAAGGGGGGAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAA	12533	0.5677044005357702	No Hit
TAAGGGGGGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	11984	0.5428364745887393	No Hit
TAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8515	0.38570198440613446	No Hit
TAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8235	0.37301888920546294	No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA	7268	0.32921691399457254	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAA	7098	0.321516463337022	No Hit
TAAGGGGGGGCAGAGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAA	6861	0.3107811291850251	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	5769	0.2613170579024063	No Hit
TAAGGGGGGGTGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	5608	0.2540242781620202	No Hit
TAAGGGGGGGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAAAA	5268	0.23862337684691912	No Hit
TAAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	5081	0.23015288112361354	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAAAA	4770	0.2160655860971534	No Hit
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGAAAAAAAAAAAAAAAAAAAAAA	3882	0.17584205560359528	No Hit
TAAGGGGGGCAGTGCTTAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3711	0.16809630817747093	No Hit
TAAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	3551	0.16084882520565866	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	3057	0.13847222153018826	Illumina Single End Adapter 2 (97% over 34bp)
TAAGGGGGGCAGAGTGCAGTGCTTAAGGGGGGTAAAAAAAAAAAAAAAAAAA	2962	0.13416902851567472	No Hit
TAAGGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTAAGGGGGGAAG	2700	0.12230127514933212	No Hit
TAAGGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2343	0.10613032876847599	Illumina Single End Adapter 2 (100% over 34bp)
TAAGGGGGAGAGTGCAGTGCTTAAGGGGGGAAAAAAAAAAAAAAAAAAAAAA	2342	0.10608503199990216	No Hit
TAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2340	0.1059944384627545	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.02133477799827238	0.0	0.0	0.0	0.0
8	0.07650624212119332	0.0	0.0	0.0	0.0
9	0.1891140087957265	0.0	0.0	0.0	0.0
10	0.4538283243411698	0.0	0.0	0.0	0.0
11	0.8034287841939644	0.0	0.0	0.0	0.0
12	0.996166534475597	0.0	0.0	0.0	0.0
13	1.1387154651774296	0.0	0.0	0.0	0.0
14	1.3010590837460247	0.0	0.0	0.0	0.0
15	1.4613190509602236	0.0	0.0	0.0	0.0
16	1.6367534356466544	0.0	0.0	0.0	0.0
17	1.785825101023118	0.0	0.0	0.0	0.0
18	1.9532872544405555	0.0	0.0	0.0	0.0
19	2.125867942706835	0.0	0.0	0.0	0.0
20	2.2928318316699605	0.0	0.0	0.0	0.0
21	2.4582556305015757	0.0	0.0	0.0	0.0
22	2.6519446129232587	0.0	0.0	0.0	0.0
23	2.8663795153517544	0.0	0.0	0.0	0.0
24	3.0900096618007367	0.0	0.0	0.0	0.0
25	3.2817508831737454	0.0	0.0	0.0	4.5296768573826714E-5
26	3.4585441709173907	0.0	0.0	0.0	4.5296768573826714E-5
27	3.6169922673886368	0.0	0.0	0.0	4.5296768573826714E-5
28	3.7661092295336744	0.0	0.0	0.0	4.5296768573826714E-5
29	3.8734625710536434	0.0	0.0	0.0	4.5296768573826714E-5
30	4.005230870834906	0.0	0.0	0.0	4.5296768573826714E-5
31	4.128075707207124	0.0	0.0	0.0	4.5296768573826714E-5
32	4.2453490410447605	0.0	0.0	0.0	4.5296768573826714E-5
33	4.368012690342684	0.0	0.0	0.0	4.5296768573826714E-5
34	4.503087654229835	0.0	0.0	0.0	4.5296768573826714E-5
35	4.650256855326198	0.0	0.0	0.0	9.059353714765343E-5
36	4.7739170335327445	0.0	0.0	0.0	9.059353714765343E-5
37	4.902741043356708	0.0	0.0	0.0	9.059353714765343E-5
38	5.040941484275454	0.0	0.0	0.0	9.059353714765343E-5
39	5.189197807817588	0.0	0.0	0.0	9.059353714765343E-5
40	5.346015220620176	0.0	0.0	0.0	9.059353714765343E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	35	1.0201438E-7	46.000004	16
ATCGTTC	35	1.0201438E-7	46.000004	22
TTACGTC	35	1.0201438E-7	46.000004	19
TACGTCG	35	1.0201438E-7	46.000004	40
ATAATAG	30	1.8621886E-6	46.0	35
TAGGTCG	20	6.3127256E-4	46.0	42
CACATCG	25	3.4181863E-5	46.0	12
TACGGAC	55	1.8189894E-12	46.0	13
TTGCGAC	100	0.0	46.0	13
ACGCTAG	25	3.4181863E-5	46.0	12
ATAACCG	85	0.0	46.0	31
CGGTCGA	20	6.3127256E-4	46.0	26
TTACCGT	30	1.8621886E-6	46.0	45
CTAGGCG	25	3.4181863E-5	46.0	28
CGATAAA	25	3.4181863E-5	46.0	24
CCGCATA	20	6.3127256E-4	46.0	19
TACGGTC	45	3.110472E-10	45.999996	18
ATTGCGA	105	0.0	43.809525	12
CGTATAT	90	0.0	43.444443	36
TAACCGT	90	0.0	43.444443	32
>>END_MODULE