FastQCFastQC Report
Fri 17 Jun 2016
SRR1527555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2089112
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC165510.792250487288379No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC112290.5375011009462394No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT104560.5004997338582134No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG103470.49528220602820716No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC77390.37044447593044316No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT76700.3671416372123658No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG63370.30333462255733534No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC59610.2853365449052037No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA58050.27786925736868107No Hit
AGTTTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG46530.22272621094512884No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA44500.21300916370208967No Hit
AGTTTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG42170.20185609962510387No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA40990.19620776674491364No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG40600.19434094486078296No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG40430.1935272019882132No Hit
AGTTTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT37780.18084238662168423No Hit
AGTTTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG36910.17667793780323887No Hit
AGTTTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT36800.17615139829745843No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC36130.17294429403497755No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC35130.1681575712551553No Hit
AGTTTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC34930.16720022669919085No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT34290.16413672412010463No Hit
AGTTTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA30800.14743106161852498No Hit
AGTTTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC30620.146569451518157No Hit
AGTTTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA29800.14264433883870276No Hit
AGTTTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT29780.14254860438310632No Hit
AGTTTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG28080.1344111756574085No Hit
AGTTTAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTT27930.13369316724043517No Hit
AGTTTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC27530.13177847812850627No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT26030.12459839395877292No Hit
AGTTTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA24640.11794484929482No Hit
AGTTTAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG24290.11626949632188222No Hit
AGTTTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC24010.11492921394353199No Hit
AGTTTAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT23400.11200931304784043No Hit
AGTTTAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT23290.11148277354205997No Hit
AGTTTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC22610.10822780205178084No Hit
AGTTTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC22600.10817993482398264No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC21750.10411122046113373No Hit
AGTTTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC21390.10238800026039771No Hit
AGTTTAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC20960.10032970946507415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATT207.857961E-444.00000429
CCGTATA254.4440952E-544.029
ATATCGT454.802132E-1044.023
GAACGTA502.7284841E-1144.010
CGGTATA254.4440952E-544.018
GTACGAT302.5287154E-644.019
ACGGTAT254.4440952E-544.017
TAGGGAT165500.042.9897275
TAGGGGC268100.042.982475
TAGGGGA395200.042.959015
AGTTTAG2138450.042.8529051
TTAGGGG1331550.042.8500634
TTTAGGG2087500.042.8365023
GTTTAGG2119250.042.8259052
TAGGGGG474100.042.6078875
AGGGATT55300.042.4882476
TAGGGGT196850.042.446535
TTAGGGC119400.042.3232844
TTAGGGA574300.042.234024
GTTTAGA19600.042.0918352
Produced by FastQC (version 0.11.5)