##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2089112
Sequences flagged as poor quality	0
Sequence length	50
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.851014689494868	30.0	25.0	31.0	16.0	33.0
2	30.59850931879191	31.0	30.0	33.0	28.0	33.0
3	27.618664772400905	28.0	25.0	30.0	23.0	31.0
4	33.95021808308985	35.0	33.0	35.0	32.0	35.0
5	34.9165674219477	35.0	35.0	35.0	35.0	37.0
6	34.29869006544408	35.0	33.0	35.0	32.0	37.0
7	35.7841800726816	36.0	35.0	37.0	35.0	37.0
8	36.525794213043625	37.0	37.0	37.0	35.0	37.0
9	38.63333368435967	39.0	39.0	39.0	38.0	39.0
10	37.47355862203654	39.0	37.0	39.0	35.0	39.0
11	37.168944987152436	39.0	37.0	39.0	34.0	39.0
12	37.03851540750328	39.0	37.0	39.0	34.0	39.0
13	37.08663298090289	39.0	37.0	39.0	34.0	39.0
14	37.87556866266624	40.0	38.0	40.0	34.0	40.0
15	37.878981117335975	40.0	38.0	40.0	34.0	40.0
16	38.027026315487156	40.0	38.0	40.0	34.0	40.0
17	37.903917549657464	40.0	37.0	40.0	34.0	40.0
18	37.7672269366123	39.0	37.0	40.0	34.0	40.0
19	37.714301100180364	39.0	37.0	40.0	34.0	40.0
20	37.54027835750309	39.0	37.0	40.0	33.0	40.0
21	37.42897221403161	39.0	36.0	40.0	33.0	40.0
22	37.2183200326263	38.0	36.0	40.0	33.0	40.0
23	37.03027745759921	38.0	36.0	40.0	33.0	40.0
24	36.88486974369972	38.0	36.0	40.0	33.0	40.0
25	36.684474073194735	38.0	35.0	40.0	32.0	40.0
26	36.050182565606825	38.0	35.0	39.0	31.0	40.0
27	35.900202095435766	38.0	34.0	39.0	31.0	40.0
28	35.75227082128675	37.0	34.0	39.0	31.0	40.0
29	35.401545249847786	37.0	34.0	38.0	30.0	40.0
30	35.08757165724001	36.0	34.0	38.0	30.0	40.0
31	34.92578904338303	36.0	33.0	38.0	30.0	40.0
32	34.878989254764704	36.0	33.0	38.0	30.0	39.0
33	34.3501095202172	36.0	33.0	38.0	29.0	39.0
34	33.83694316053902	35.0	32.0	38.0	27.0	39.0
35	33.70229504210401	35.0	32.0	38.0	27.0	39.0
36	33.31447428381054	35.0	31.0	38.0	26.0	38.0
37	33.108870180248836	34.0	31.0	37.0	26.0	38.0
38	32.6538036256553	34.0	31.0	37.0	25.0	38.0
39	31.814323023370694	33.0	30.0	36.0	23.0	38.0
40	31.51987734501549	33.0	29.0	36.0	23.0	38.0
41	31.178082840939116	33.0	29.0	36.0	23.0	38.0
42	30.87902563385783	32.0	29.0	35.0	23.0	38.0
43	30.369497183492317	32.0	28.0	35.0	22.0	37.0
44	29.87839474379545	31.0	27.0	34.0	21.0	37.0
45	29.345753123815285	31.0	26.0	34.0	20.0	37.0
46	29.214293441423916	31.0	26.0	34.0	20.0	37.0
47	28.650055143046423	30.0	26.0	34.0	19.0	36.0
48	28.111429162246925	30.0	24.0	33.0	18.0	36.0
49	27.37959525386863	29.0	24.0	33.0	17.0	35.0
50	31.264696674950887	33.0	29.0	35.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	5.0
14	21.0
15	69.0
16	150.0
17	301.0
18	564.0
19	968.0
20	1491.0
21	2455.0
22	3799.0
23	5910.0
24	8658.0
25	12477.0
26	17755.0
27	25314.0
28	35258.0
29	49485.0
30	69000.0
31	98871.0
32	153135.0
33	213689.0
34	340329.0
35	585074.0
36	424116.0
37	39290.0
38	920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.68374122593714	0.25460578465874495	0.05006912027694063	0.011583869127169822
8	98.99971854070054	0.8989465380506166	0.08726195627615944	0.014072964972677387
9	98.4464212545809	0.95432892061316	0.19371867089940606	0.40553115390654026
10	63.67250774491746	27.083564691601026	3.4584072084215682	5.785520355059949
11	35.51619061113047	25.837820088152284	19.867867304385786	18.778121996331457
12	29.483675360631693	18.189977368374695	26.685452958003207	25.640894312990397
13	24.943612405653695	17.30553460034694	29.641158540087847	28.109694453911516
14	26.071603628718805	17.68607906134281	30.455475819391204	25.78684149054718
15	25.440378495743648	17.52859588188666	28.88490420810373	28.146121414265966
16	30.720755995848954	17.497242847678823	25.437697930986946	26.344303225485277
17	31.214267114448628	18.389823044432276	23.79417666453498	26.60173317658412
18	28.87987814918492	18.6314568103577	23.64435224152654	28.844312798930837
19	29.962969912575293	19.062501196680696	23.47102500966918	27.50350388107483
20	29.278995094566497	21.816542148051422	21.965887898781876	26.938574858600212
21	30.516889472656324	19.42691440190856	24.429614113556383	25.626582011878728
22	30.778771075940398	18.50953898115563	24.156962384017707	26.554727558886267
23	29.755465480070004	19.31471361994953	23.702127985479	27.22769291450147
24	29.775761184656446	19.924972907149066	22.882066638839852	27.417199269354636
25	30.112746468355933	19.774526210179253	22.503963406461693	27.60876391500312
26	29.123474471450074	19.46171387651787	22.28913528810327	29.125676363928786
27	28.13013376018136	18.36311313132087	22.764887665189804	30.74186544330797
28	30.781643109608293	19.768638541160072	21.50420848666802	27.94550986256362
29	31.320628094616275	20.507325600542238	21.014526746292205	27.157519558549275
30	29.655040036149334	19.175611455967896	22.968706321154635	28.200642186728142
31	32.238386453191595	20.17306874882725	21.052437590708397	26.536107207272757
32	32.968457411570085	20.38320587886145	21.91720692811108	24.731129781457383
33	33.87893995152007	19.5417478813965	21.430062150808574	25.14925001627486
34	30.40636404367023	21.95262867668177	23.883209708239672	23.757797571408332
35	29.650301180597303	23.48370982503571	23.801021678110125	23.06496731625686
36	30.94927414135767	22.90035191985877	20.977142441381794	25.173231497401765
37	29.955359023355378	24.044187195325094	20.907112687112996	25.093341094206533
38	32.202198828976144	22.68207736109888	20.457639418087687	24.6580843918373
39	31.793125500212533	21.246826402796977	20.686779837557776	26.273268259432715
40	30.129356396401917	21.583715952040865	21.82645066420565	26.460476987351562
41	27.809471201161067	22.588544797981154	22.468062985613027	27.133921015244756
42	27.879836026024456	19.956230206901303	22.735592921777293	29.428340845296947
43	27.328261960105536	18.919186716652817	25.68229946503586	28.070251858205786
44	27.538351222911935	18.30466724617924	24.768705555278988	29.388275975629835
45	28.027506423781972	19.468654624548613	24.339575858067924	28.164263093601495
46	28.710045224956826	20.319542465889814	23.070472047453656	27.899940261699708
47	27.996488460168724	21.493246891502228	23.69322468110853	26.817039967220524
48	28.42466081282382	21.994656102688605	24.250973619413415	25.329709465074156
49	28.629197477205626	20.788354094945603	23.916094493737052	26.66635393411172
50	26.720060963701325	21.392629978670364	24.048208042460146	27.839101015168165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	5.0
4	6.0
5	11.5
6	17.0
7	232.0
8	447.0
9	857.5
10	1268.0
11	1692.5
12	2117.0
13	1962.5
14	1808.0
15	2079.0
16	2350.0
17	2665.0
18	2980.0
19	3078.0
20	3176.0
21	4338.0
22	5500.0
23	5893.5
24	6287.0
25	7223.0
26	8159.0
27	8723.0
28	9287.0
29	12248.0
30	15209.0
31	16347.0
32	17485.0
33	20170.5
34	22856.0
35	26461.0
36	30066.0
37	33305.0
38	36544.0
39	39714.0
40	42884.0
41	49409.5
42	55935.0
43	61156.0
44	66377.0
45	73990.5
46	81604.0
47	89597.5
48	97591.0
49	101937.5
50	106284.0
51	126802.0
52	147320.0
53	173163.0
54	199006.0
55	205406.0
56	211806.0
57	208407.0
58	205008.0
59	207904.5
60	210801.0
61	181461.5
62	152122.0
63	130271.0
64	108420.0
65	90484.5
66	72549.0
67	65274.0
68	57999.0
69	48994.5
70	39990.0
71	42131.5
72	44273.0
73	29987.0
74	15701.0
75	9988.5
76	4276.0
77	3568.0
78	2860.0
79	1722.0
80	584.0
81	351.5
82	119.0
83	70.0
84	21.0
85	16.0
86	11.0
87	8.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2089112.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.24441966676047
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.94258304000113	7.165211732390148
2	9.892436324416094	2.026845384680478
3	3.558197312860682	1.0935499959025268
4	1.7147634499262068	0.7026702564106426
5	0.9413183051992611	0.48216298792324713
6	0.6064924604550475	0.372789797377658
7	0.45342696979422564	0.3251567316759699
8	0.3334718317502355	0.27329803131942004
9	0.30374005540790433	0.28004765374832846
>10	7.736578593383478	22.272163847168244
>50	3.011957624459296	21.18918516644549
>100	1.3935500299503993	27.26480778618732
>500	0.06991369641785056	4.909675671563856
>1k	0.037318797412231035	7.6695625507028335
>5k	0.002361949203305762	1.6217662474786152
>10k+	0.0018895593626446095	2.3511061590252313
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	16551	0.792250487288379	No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	11229	0.5375011009462394	No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	10456	0.5004997338582134	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	10347	0.49528220602820716	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	7739	0.37044447593044316	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	7670	0.3671416372123658	No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	6337	0.30333462255733534	No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5961	0.2853365449052037	No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	5805	0.27786925736868107	No Hit
AGTTTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	4653	0.22272621094512884	No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	4450	0.21300916370208967	No Hit
AGTTTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	4217	0.20185609962510387	No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	4099	0.19620776674491364	No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	4060	0.19434094486078296	No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	4043	0.1935272019882132	No Hit
AGTTTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	3778	0.18084238662168423	No Hit
AGTTTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	3691	0.17667793780323887	No Hit
AGTTTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	3680	0.17615139829745843	No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	3613	0.17294429403497755	No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	3513	0.1681575712551553	No Hit
AGTTTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3493	0.16720022669919085	No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	3429	0.16413672412010463	No Hit
AGTTTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	3080	0.14743106161852498	No Hit
AGTTTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	3062	0.146569451518157	No Hit
AGTTTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2980	0.14264433883870276	No Hit
AGTTTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	2978	0.14254860438310632	No Hit
AGTTTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2808	0.1344111756574085	No Hit
AGTTTAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTT	2793	0.13369316724043517	No Hit
AGTTTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	2753	0.13177847812850627	No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2603	0.12459839395877292	No Hit
AGTTTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA	2464	0.11794484929482	No Hit
AGTTTAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAG	2429	0.11626949632188222	No Hit
AGTTTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	2401	0.11492921394353199	No Hit
AGTTTAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	2340	0.11200931304784043	No Hit
AGTTTAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT	2329	0.11148277354205997	No Hit
AGTTTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2261	0.10822780205178084	No Hit
AGTTTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	2260	0.10817993482398264	No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	2175	0.10411122046113373	No Hit
AGTTTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	2139	0.10238800026039771	No Hit
AGTTTAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC	2096	0.10032970946507415	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.015461114578825836	0.0	0.0	0.0	0.0
7	0.015604716262220503	0.0	0.0	0.0	0.0
8	0.016226990223597394	0.0	0.0	0.0	0.0
9	0.017423670918552955	0.0	0.0	0.0	0.0
10	0.032836918269580566	0.0	0.0	0.0	0.0
11	0.05739280613006866	0.0	0.0	0.0	0.0
12	0.07113070050815849	0.0	0.0	0.0	0.0
13	0.08137428725697808	0.0	0.0	0.0	0.0
14	0.09334109420653368	0.0	0.0	0.0	0.0
15	0.10435055660012484	0.0	0.0	0.0	0.0
16	0.11435480720995332	0.0	0.0	0.0	0.0
17	0.12273157207464223	0.0	0.0	0.0	0.0
18	0.12952871842198982	0.0	0.0	0.0	0.0
19	0.14053818081558098	0.0	0.0	0.0	0.0
20	0.1495372196416468	0.0	0.0	0.0	0.0
21	0.15954147025147528	0.0	0.0	0.0	0.0
22	0.16696089056019975	0.0	0.0	0.0	0.0
23	0.1771087428534229	0.0	0.0	0.0	0.0
24	0.1865385867296727	0.0	0.0	0.0	0.0
25	0.1970215096174834	0.0	0.0	0.0	0.0
26	0.2065949551771279	0.0	0.0	0.0	0.0
27	0.21746081588732438	0.0	0.0	0.0	0.0
28	0.23531529185606134	0.0	0.0	0.0	0.0
29	0.24522380801029336	0.0	0.0	0.0	0.0
30	0.2561854031760863	0.0	0.0	0.0	0.0
31	0.26695552943068634	0.0	0.0	0.0	0.0
32	0.27935314143042594	0.0	0.0	0.0	0.0
33	0.2866289600557557	0.0	0.0	0.0	0.0
34	0.29711188294356644	0.0	0.0	0.0	0.0
35	0.3121421924722083	0.0	0.0	0.0	0.0
36	0.3254492818001141	0.0	0.0	0.0	0.0
37	0.3354056651821444	0.0	0.0	0.0	0.0
38	0.34651086203133197	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATT	20	7.857961E-4	44.000004	29
CCGTATA	25	4.4440952E-5	44.0	29
ATATCGT	45	4.802132E-10	44.0	23
GAACGTA	50	2.7284841E-11	44.0	10
CGGTATA	25	4.4440952E-5	44.0	18
GTACGAT	30	2.5287154E-6	44.0	19
ACGGTAT	25	4.4440952E-5	44.0	17
TAGGGAT	16550	0.0	42.989727	5
TAGGGGC	26810	0.0	42.98247	5
TAGGGGA	39520	0.0	42.95901	5
AGTTTAG	213845	0.0	42.852905	1
TTAGGGG	133155	0.0	42.850063	4
TTTAGGG	208750	0.0	42.836502	3
GTTTAGG	211925	0.0	42.825905	2
TAGGGGG	47410	0.0	42.607887	5
AGGGATT	5530	0.0	42.488247	6
TAGGGGT	19685	0.0	42.44653	5
TTAGGGC	11940	0.0	42.323284	4
TTAGGGA	57430	0.0	42.23402	4
GTTTAGA	1960	0.0	42.091835	2
>>END_MODULE