##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527554_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1653042
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.43308820949498	31.0	30.0	33.0	28.0	33.0
2	31.520981318079034	31.0	31.0	33.0	30.0	33.0
3	29.3174759020037	30.0	28.0	31.0	25.0	33.0
4	34.58629423813793	35.0	35.0	35.0	33.0	35.0
5	35.404240787590396	35.0	35.0	37.0	35.0	37.0
6	34.9265535902899	35.0	35.0	37.0	33.0	37.0
7	36.07290316882451	37.0	35.0	37.0	35.0	37.0
8	36.635141151888455	37.0	37.0	37.0	35.0	37.0
9	38.75547324266413	39.0	39.0	39.0	39.0	39.0
10	37.86656842354883	39.0	38.0	39.0	35.0	39.0
11	37.73275633649962	39.0	37.0	39.0	35.0	39.0
12	37.60106155802454	39.0	37.0	39.0	35.0	39.0
13	37.60595012104955	39.0	37.0	39.0	35.0	39.0
14	38.37288647233404	40.0	38.0	40.0	35.0	40.0
15	38.43321464306412	40.0	38.0	40.0	35.0	40.0
16	38.565214313973875	40.0	38.0	40.0	35.0	40.0
17	38.475643087108494	40.0	38.0	40.0	35.0	40.0
18	38.37151324648738	40.0	38.0	40.0	35.0	40.0
19	38.30209456263059	40.0	38.0	40.0	35.0	40.0
20	38.22079838261823	40.0	38.0	40.0	34.0	40.0
21	38.18700492788447	40.0	38.0	40.0	34.0	40.0
22	38.17414681538642	40.0	38.0	40.0	34.0	40.0
23	38.05487398384312	40.0	38.0	40.0	34.0	40.0
24	38.00803125389434	40.0	38.0	40.0	34.0	40.0
25	38.01870248910796	40.0	38.0	40.0	34.0	40.0
26	37.95927084732269	40.0	38.0	40.0	34.0	40.0
27	37.92085319066303	40.0	37.0	40.0	34.0	40.0
28	37.777369238047186	40.0	37.0	40.0	34.0	40.0
29	37.695394309400484	40.0	37.0	40.0	34.0	40.0
30	37.55155041432704	39.0	37.0	40.0	33.0	40.0
31	37.50700587159915	39.0	37.0	40.0	33.0	40.0
32	37.3960117165807	39.0	36.0	40.0	33.0	40.0
33	37.1983168001781	39.0	36.0	40.0	33.0	40.0
34	36.81198783817955	39.0	35.0	40.0	31.0	40.0
35	36.66342234498579	39.0	35.0	40.0	31.0	40.0
36	36.59324324487823	39.0	35.0	40.0	31.0	40.0
37	36.45709606894441	38.0	35.0	40.0	31.0	40.0
38	36.61720633837495	39.0	35.0	40.0	31.0	40.0
39	36.5395882258285	39.0	35.0	40.0	31.0	40.0
40	36.29758711514892	38.0	35.0	40.0	31.0	40.0
41	36.16162081786186	38.0	35.0	40.0	31.0	40.0
42	36.057558126169816	38.0	35.0	40.0	30.0	40.0
43	36.00316265406445	38.0	35.0	40.0	30.0	40.0
44	35.861947851294765	38.0	35.0	40.0	30.0	40.0
45	35.71084945210104	38.0	34.0	40.0	30.0	40.0
46	35.553431794231486	37.0	34.0	40.0	30.0	40.0
47	35.39939033611971	37.0	34.0	40.0	29.0	40.0
48	35.13335656323312	37.0	34.0	40.0	29.0	40.0
49	34.95441676618017	36.0	34.0	40.0	28.0	40.0
50	35.43014091595979	36.0	34.0	40.0	30.0	40.0
51	35.52284817929611	36.0	35.0	40.0	31.0	40.0
52	35.16993639605043	36.0	34.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	4.0
14	14.0
15	35.0
16	53.0
17	90.0
18	208.0
19	356.0
20	585.0
21	929.0
22	1559.0
23	2373.0
24	3459.0
25	4803.0
26	6576.0
27	9065.0
28	11849.0
29	16021.0
30	21114.0
31	30071.0
32	42378.0
33	65271.0
34	119047.0
35	120063.0
36	213299.0
37	360998.0
38	591650.0
39	31168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.69849525904363	0.2471201578665273	0.04174122617574145	0.012643356914101395
8	99.0092205763677	0.908990818140132	0.0705366227839341	0.011251982708243348
9	98.49265777881023	0.9369997858493615	0.17688600773604057	0.3934564276043803
10	63.903760461016724	26.842330684882782	3.4456474790114227	5.808261375089079
11	35.51174138346152	25.772182436985876	19.916009393590723	18.80006678596188
12	29.44547083498181	18.14128134675344	26.8531592058762	25.560088612388554
13	25.099664739310917	17.294841873346233	29.692530498317648	27.912962889025206
14	26.24053109358383	17.575355012153352	30.369343307671553	25.81477058659127
15	25.345333028440898	17.423513740122758	29.032898135679552	28.198255095756792
16	30.68512475787064	17.524721089966256	25.38719524367802	26.402958908485086
17	31.313178975488825	18.22815149282353	23.832243826835615	26.626425704852025
18	28.82679326962049	18.56492454517187	23.67568398141124	28.932598203796395
19	30.140613487134626	18.903330949848822	23.389484356719308	27.56657120629724
20	29.395925814347123	21.654319732952942	22.00645839609641	26.943296056603522
21	30.60127933833502	19.31675057258073	24.429445833802166	25.652524255282078
22	30.858683566418758	18.479264289715566	24.18432199544839	26.477730148417283
23	29.92247020946836	19.086508388776572	23.72910065201005	27.261920749745016
24	29.90637866430496	19.66162989204146	22.927427131313056	27.504564312340523
25	30.19784131316688	19.617105917454005	22.619207497450155	27.565845271928964
26	29.167619455525024	19.247726313064035	22.431795441374145	29.152858790036795
27	28.253789074929735	18.260879033926543	22.747395407981163	30.73793648316256
28	30.767336825077646	19.632048066534306	21.511794618648526	28.088820489739525
29	31.2960590233037	20.426401749017874	20.97175994318354	27.30577928449489
30	29.610862881886852	19.037084357203266	23.02815052491104	28.323902235998844
31	32.39113101784468	19.972511285254697	21.040723708169544	26.595633988731077
32	33.067338881891686	20.26361096693248	21.90016950567499	24.76888064550084
33	33.93809715663607	19.374643838450567	21.478522626769315	25.208736378144053
34	30.55736030905446	21.70924876681899	23.951236568701823	23.782154355424726
35	29.775287016300855	23.268616284401727	23.780883970280247	23.17521272901717
36	30.958136574872263	22.75804244538251	21.09135763035664	25.192463349388582
37	30.17503487509694	23.81578931448808	20.894205954839624	25.11496985557536
38	32.29464224139495	22.535785539629362	20.421985648277538	24.747586570698143
39	31.852366727524167	20.98301192589178	20.77690705983272	26.387714286751336
40	30.212783462247177	21.42401705461809	21.815658646301788	26.547540836832944
41	27.91550365931416	22.377047891100165	22.538205320856942	27.16924312872873
42	28.081198178872647	19.75134328105396	22.81412087533166	29.35333766474173
43	27.492888867917454	18.684401243283595	25.721548514798776	28.101161374000178
44	27.670440315491074	18.123253976607977	24.916910762098	29.28939494580295
45	28.110054070011532	19.245064553713696	24.31620007235146	28.328681303923315
46	28.76442340847964	20.029678616756257	23.17769300477544	28.028204969988664
47	28.088518017086074	21.234729668090708	23.861583674220014	26.815168640603204
48	28.58027805706086	21.715056241765183	24.386373727951256	25.3182919732227
49	28.69437074194122	20.450962528477802	24.18916155790355	26.665505171677427
50	26.719466293052445	21.34065559132799	24.060852658311163	27.879025457308405
51	26.785465826034667	22.380556573880156	23.485549671454205	27.348427928630976
52	28.060630038438223	21.69757332239592	23.12252199278663	27.11927464637922
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	1.5
4	1.0
5	4.0
6	7.0
7	187.0
8	367.0
9	821.5
10	1276.0
11	1457.0
12	1638.0
13	1442.5
14	1535.5
15	1824.0
16	1920.0
17	2016.0
18	1999.5
19	1983.0
20	2495.0
21	3007.0
22	3382.5
23	3758.0
24	4925.0
25	6092.0
26	5564.5
27	5037.0
28	7108.0
29	9179.0
30	10487.0
31	11795.0
32	12954.0
33	14113.0
34	16756.5
35	19400.0
36	22124.0
37	24848.0
38	26886.0
39	31413.5
40	33903.0
41	38606.0
42	43309.0
43	47536.0
44	51763.0
45	57047.5
46	62332.0
47	66915.0
48	71498.0
49	76881.0
50	82264.0
51	93998.5
52	105733.0
53	126409.5
54	147086.0
55	155548.5
56	164011.0
57	158056.0
58	152101.0
59	159126.0
60	166151.0
61	144731.0
62	123311.0
63	111904.0
64	80962.0
65	61427.0
66	55486.5
67	49546.0
68	43012.0
69	36478.0
70	36966.0
71	37454.0
72	27942.5
73	18431.0
74	11695.0
75	4959.0
76	3609.5
77	2260.0
78	1982.5
79	1705.0
80	958.0
81	211.0
82	135.0
83	59.0
84	40.5
85	22.0
86	15.0
87	8.0
88	8.5
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1653042.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.338227458037812
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.89354795863296	6.1532893885483775
2	9.798223101069581	1.8299607200477672
3	3.607424118187561	1.0106084085974079
4	1.6636546780381178	0.6214234318059443
5	0.9496090581845347	0.4433832690770125
6	0.661860697846041	0.3708363445213181
7	0.5342258146638057	0.3492105519900319
8	0.4737170830886158	0.35389422981117535
9	0.4468905894968473	0.37558493762203476
>10	11.357258406638957	28.65569738430851
>50	3.0596814149860103	19.318110550104333
>100	1.4446327429175019	25.514541787875615
>500	0.06764601767826213	4.427080920602629
>1k	0.03707522122750905	7.122956117211403
>5k	0.003902654866053584	2.674251828503314
>10k+	6.504424776755974E-4	0.7791701293731378
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	12828	0.776023839684654	No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	8660	0.5238826357709	No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8392	0.5076701015461192	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8392	0.5076701015461192	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	6930	0.4192270976780989	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	6051	0.3660524052020457	No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5603	0.3389508554531585	No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4747	0.2871675371829633	No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4544	0.27488714745299875	No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3825	0.2313915798872624	No Hit
AGTTTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3710	0.22443470885797215	No Hit
AGTTTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3336	0.20180975438010648	No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	3322	0.20096283095045378	No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3314	0.20047887470493794	No Hit
AGTTTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3297	0.19945046768321678	No Hit
AGTTTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3171	0.19182815681634224	No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	3126	0.18910590293531562	No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3048	0.18438732954153614	No Hit
AGTTTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2929	0.177188480389488	No Hit
AGTTTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2880	0.17422424838570344	No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	2821	0.1706550710750241	No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2809	0.16992913670675036	No Hit
AGTTTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	2731	0.16521056331297088	No Hit
AGTTTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2600	0.15728577979264893	No Hit
AGTTTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	2500	0.1512363267237009	No Hit
AGTTTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2473	0.14960297439508494	No Hit
AGTTTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2413	0.1459733025537161	No Hit
AGTTTAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTTAC	2190	0.132483022209962	No Hit
AGTTTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	2147	0.12988175739031435	No Hit
AGTTTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2098	0.1269175253865298	No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2068	0.1251026894658454	No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2026	0.1225619191768872	No Hit
AGTTTAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	2006	0.12135202856309761	No Hit
AGTTTAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	1929	0.11669394970000761	No Hit
AGTTTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	1919	0.11608900439311282	No Hit
AGTTTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1916	0.11590752080104438	No Hit
AGTTTAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	1805	0.10919262789451205	No Hit
AGTTTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1799	0.10882966071037517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.017059457654433462	0.0	0.0	0.0	0.0
7	0.017482919369259824	0.0	0.0	0.0	0.0
8	0.01814835920684411	0.0	0.0	0.0	0.0
9	0.018874293575117874	0.0	0.0	0.0	0.0
10	0.03442138796231433	0.0	0.0	0.0	0.0
11	0.06025255256672244	0.0	0.0	0.0	0.0
12	0.07489222899357668	0.0	0.0	0.0	0.0
13	0.08487382655734095	0.0	0.0	0.0	0.0
14	0.09781965612488974	0.0	0.0	0.0	0.0
15	0.10864817711830674	0.0	0.0	0.0	0.0
16	0.11850878562069203	0.0	0.0	0.0	0.0
17	0.12758296522411408	0.0	0.0	0.0	0.0
18	0.13653615576615719	0.0	0.0	0.0	0.0
19	0.1485140728426743	0.0	0.0	0.0	0.0
20	0.1577697360381648	0.0	0.0	0.0	0.0
21	0.16666243204951842	0.0	0.0	0.0	0.0
22	0.1752526554074246	0.0	0.0	0.0	0.0
23	0.18468980219498354	0.0	0.0	0.0	0.0
24	0.19491337788150573	0.0	0.0	0.0	0.0
25	0.2033221176473435	0.0	0.0	0.0	0.0
26	0.21221481365869713	0.0	0.0	0.0	0.0
27	0.22171245497694553	0.0	0.0	0.0	0.0
28	0.2384694399779316	0.0	0.0	0.0	0.0
29	0.24705966333583781	0.0	0.0	0.0	0.0
30	0.2588560968202865	0.0	0.0	0.0	0.0
31	0.26726483658612427	0.0	0.0	0.0	0.0
32	0.2803921497457415	0.0	0.0	0.0	0.0
33	0.2871675371829633	0.0	0.0	0.0	0.0
34	0.29872199254465404	0.0	0.0	0.0	0.0
35	0.31342216350219776	0.0	0.0	0.0	0.0
36	0.32606552041629916	0.0	0.0	0.0	0.0
37	0.3377409648393689	0.0	0.0	0.0	0.0
38	0.34729910068830677	0.0	0.0	0.0	0.0
39	0.35480042249380234	0.0	0.0	0.0	0.0
40	0.3636326239744665	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGGTA	85	0.0	46.0	4
TACGGTA	55	1.8189894E-12	46.0	43
CACTACG	20	6.312262E-4	46.0	46
CGTGTAT	20	6.312262E-4	46.0	15
CGCGTAA	20	6.312262E-4	46.0	33
ACGAACG	30	1.8619012E-6	46.0	45
CGATAAC	35	1.01992555E-7	45.999996	40
TAGGGAT	12685	0.0	44.912098	5
TAGGGGA	31290	0.0	44.8092	5
TAGGGGC	22065	0.0	44.769997	5
AGTTTAG	169320	0.0	44.74758	1
TTAGGGG	105845	0.0	44.739666	4
GTTTAGG	167855	0.0	44.724316	2
TTTAGGG	165405	0.0	44.717937	3
TAGGGGG	37290	0.0	44.59989	5
TAGGGGT	15425	0.0	44.24052	5
TTAGGGC	9715	0.0	44.224392	4
TTAGGGA	44740	0.0	44.21614	4
AGGGATT	4125	0.0	44.16	6
AGGGGAG	14635	0.0	43.81551	6
>>END_MODULE