FastQCFastQC Report
Fri 17 Jun 2016
SRR1527553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219036
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC169060.7618623582492579No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT120190.5416315913757145No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG115200.5191443491678369No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT111390.5019747313698381No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA86640.3904398126033106No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC75530.34037302684589166No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG69950.31522697243307457No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC65760.2963448993166402No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC63190.28476329361037855No Hit
AGTTTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG50160.22604410203349562No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA49320.22225867448748016No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG46020.2073873519852765No Hit
AGTTTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC45290.20409763518933446No Hit
AGTTTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT43650.19670703855187566No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT42770.19274135255128805No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA42460.19134434952835377No Hit
AGTTTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC40840.18404388211818104No Hit
AGTTTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC39720.1789966453901604No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG39710.17895158077651738No Hit
AGTTTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG38010.17129059645720032No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT37480.16890217193411913No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA35350.15930340922815134No Hit
AGTTTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC34940.15745576006878662No Hit
AGTTTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG33570.15128190799968996No Hit
AGTTTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG33340.1502454218859No Hit
AGTTTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC32710.1474063512263884No Hit
AGTTTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA32400.14600934820345413No Hit
AGTTTAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTTAC30450.13722174854306105No Hit
AGTTTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT30350.13677110240663062No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT28200.12708221047337673No Hit
AGTTTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA27790.12523456131401203No Hit
AGTTTAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG25860.11653709088090504No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT25650.11559073399440119No Hit
AGTTTAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA25550.11514008785797075No Hit
AGTTTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT25100.1131121802440339No Hit
AGTTTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT25090.11306711563039086No Hit
AGTTTAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG24800.11176024183474265No Hit
AGTTTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG24470.11027310958452229No Hit
AGTTTAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC22360.10076447610584055No Hit
AGTTTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA22280.10040395919669622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT253.4181914E-546.024
CACTACG206.3127314E-446.046
CGTGTAT253.4181914E-546.015
ACCGTTT453.110472E-1046.019
ACGTATA206.3127314E-446.040
CGACTAA206.3127314E-446.023
AATAACG453.110472E-1046.046
TAGGGGC279100.044.9122165
TAGGGGA426500.044.8136025
AGTTTAG2273950.044.762991
TAGGGAT172500.044.765
TTAGGGG1418200.044.7269064
GTTTAGG2256400.044.7105562
TTTAGGG2222450.044.710523
TAGGGGT214600.044.4566655
TAGGGGG500950.044.4022335
AGGGATT58450.044.1899036
TTAGGGA611200.044.125984
TAGGGAG228350.044.0459825
CGACAAA1150.044.043
Produced by FastQC (version 0.11.5)