##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2219036
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.186175888989634	30.0	28.0	31.0	23.0	33.0
2	30.47073774377703	31.0	30.0	33.0	28.0	33.0
3	27.74733397745688	30.0	26.0	30.0	25.0	31.0
4	33.992910434981674	35.0	33.0	35.0	32.0	35.0
5	34.89323021348009	35.0	35.0	35.0	35.0	37.0
6	34.1648594254442	35.0	33.0	35.0	32.0	37.0
7	35.70651354912674	35.0	35.0	37.0	35.0	37.0
8	36.420192822468856	37.0	36.0	37.0	35.0	37.0
9	38.635234399081405	39.0	39.0	39.0	37.0	39.0
10	37.37301738232277	39.0	37.0	39.0	34.0	39.0
11	37.05925320724855	39.0	37.0	39.0	34.0	39.0
12	36.94010101683794	39.0	35.0	39.0	33.0	39.0
13	36.91744388103663	39.0	37.0	39.0	33.0	39.0
14	37.682961880744614	40.0	37.0	40.0	33.0	40.0
15	37.747333977456876	40.0	37.0	40.0	33.0	40.0
16	37.85723981044021	40.0	37.0	40.0	33.0	40.0
17	37.79631831119459	40.0	37.0	40.0	33.0	40.0
18	37.73400251280286	40.0	37.0	40.0	33.0	40.0
19	37.62836835454675	40.0	37.0	40.0	33.0	40.0
20	37.50256282457788	39.0	37.0	40.0	33.0	40.0
21	37.545545002424475	39.0	37.0	40.0	33.0	40.0
22	37.526873381053754	39.0	37.0	40.0	33.0	40.0
23	37.462569782554226	39.0	36.0	40.0	33.0	40.0
24	37.4423839901651	39.0	36.0	40.0	33.0	40.0
25	37.44191667012162	39.0	36.0	40.0	33.0	40.0
26	37.31818771754942	39.0	36.0	40.0	33.0	40.0
27	37.2437333148268	39.0	36.0	40.0	33.0	40.0
28	37.04688297080354	39.0	36.0	40.0	32.0	40.0
29	36.992791915047796	39.0	35.0	40.0	32.0	40.0
30	36.884713452147686	39.0	35.0	40.0	32.0	40.0
31	36.80143494742762	39.0	35.0	40.0	32.0	40.0
32	36.72699226150455	38.0	35.0	40.0	31.0	40.0
33	36.59039060204521	38.0	35.0	40.0	31.0	40.0
34	36.28393590730389	38.0	35.0	40.0	30.0	40.0
35	36.124114255018846	38.0	35.0	40.0	30.0	40.0
36	36.08810582613351	38.0	35.0	40.0	30.0	40.0
37	35.98825751362303	38.0	35.0	40.0	30.0	40.0
38	35.905575213741464	38.0	34.0	40.0	30.0	40.0
39	35.73915790460362	38.0	34.0	40.0	30.0	40.0
40	36.00242627879854	38.0	35.0	40.0	30.0	40.0
41	36.08339927788463	38.0	35.0	40.0	31.0	40.0
42	36.16279186097026	38.0	35.0	40.0	31.0	40.0
43	36.10977469495763	38.0	35.0	40.0	31.0	40.0
44	35.99498926560903	38.0	35.0	40.0	31.0	40.0
45	35.88200642080615	38.0	35.0	40.0	31.0	40.0
46	35.6359171279781	37.0	34.0	40.0	30.0	40.0
47	35.55063324795091	37.0	34.0	40.0	30.0	40.0
48	35.39913683239028	37.0	34.0	40.0	30.0	40.0
49	35.216000551590874	36.0	34.0	40.0	30.0	40.0
50	35.09625531086472	36.0	34.0	39.0	29.0	40.0
51	34.923133288509064	36.0	34.0	39.0	29.0	40.0
52	34.48990913171305	35.0	33.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	4.0
14	8.0
15	28.0
16	66.0
17	143.0
18	319.0
19	575.0
20	1092.0
21	1764.0
22	2800.0
23	4255.0
24	6371.0
25	8924.0
26	12538.0
27	17051.0
28	22765.0
29	30438.0
30	40355.0
31	55054.0
32	78437.0
33	116924.0
34	177111.0
35	194204.0
36	332558.0
37	515109.0
38	589499.0
39	10638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.71136114961632	0.24515149821814516	0.03334781409585063	0.010139538069684314
8	99.0278661544923	0.8958394546100198	0.06381149291854661	0.012482897979122467
9	98.4768160588652	0.9546487754141889	0.17174124259363074	0.3967939231269794
10	63.844525280346964	26.949405056970683	3.366011186839691	5.840058475842663
11	35.39235055222177	25.916253724590316	19.84352664850863	18.847869074679274
12	29.32877159270963	18.179065143602898	26.75332892300981	25.738834340677663
13	24.791756420355508	17.36537847966414	29.721915282131523	28.120949817848835
14	26.0442822919502	17.62999789097608	30.49774767061012	25.8279721464636
15	25.34167990064154	17.492686013205734	28.952617262631158	28.213016823521563
16	30.642360015790644	17.431308009423912	25.391566427944383	26.53476554684106
17	31.17578984748332	18.332374959216523	23.777397031864286	26.714438161435865
18	28.758614100897866	18.554182987567575	23.714847348127748	28.972355563406815
19	29.94318253511885	19.013932175953883	23.427605500766997	27.61527978816026
20	29.271990179519396	21.688922577191178	21.991621587031485	27.04746565625794
21	30.515412999158194	19.285446473153208	24.438765301689564	25.760375225999038
22	30.801663425018795	18.43219307843586	24.163600770785152	26.6025427257602
23	29.74431239511211	19.196533990435487	23.6931262043518	27.366027410100603
24	29.759363976068887	19.792603635091996	22.925811027851733	27.52222136098738
25	30.127136288009748	19.666783233800622	22.551864863841775	27.654215614347855
26	29.124133182156577	19.30090363563277	22.372282378474257	29.202680803736396
27	28.15303582276268	18.333050928421173	22.769031236987594	30.74488201182856
28	30.837444728251366	19.628388182976753	21.560578557535795	27.97358853123609
29	31.346990314713235	20.405752768319214	20.98415708442765	27.263099832539893
30	29.569236371108897	19.066838032370814	23.02517850093464	28.33874709558565
31	32.24066666786839	20.200979163925236	20.93314394178373	26.625210226422645
32	32.92006078315089	20.416342952525333	21.924114795794207	24.73948146852958
33	33.80715770271415	19.450788540609526	21.43038688872105	25.311666867955275
34	30.43803705753309	21.889415043289066	23.856305170353252	23.816242728824587
35	29.648234638825148	23.33990976261764	23.75369304508805	23.258162553469163
36	30.878768978962036	22.86348666718341	21.067346361212707	25.19039799264185
37	30.006272994219113	23.912185291270625	20.88663726050411	25.194904454006156
38	32.2043445892721	22.54104935656745	20.483218839171606	24.771387214988852
39	31.737430127316546	21.0978100400354	20.787404981262135	26.377354851385913
40	30.208522980249082	21.46256302286218	21.829479107143822	26.499434889744915
41	27.915770631932062	22.443529532643904	22.51581317292734	27.12488666249669
42	27.822081300168183	19.832035172029656	22.858754882750887	29.487128645051275
43	27.378059661943293	18.740480100367908	25.82842279260003	28.05303744508877
44	27.557236565788028	18.107592666365036	24.937810833172602	29.397359934674338
45	28.03812105797292	19.348897449162607	24.368915150542847	28.244066342321624
46	28.830852676567666	20.234281913407443	23.141805721042832	27.793059688982062
47	28.071784324364273	21.329171766478776	23.775324059636706	26.823719849520245
48	28.495301563381574	21.722360520514314	24.422046330027992	25.360291586076116
49	28.671954848862296	20.506111662902267	24.187485016015962	26.634448472219468
50	26.678161147453217	21.41353272321855	24.056977894905714	27.851328234422517
51	26.747560652463502	22.441411495802683	23.43468064510896	27.376347206624857
52	27.989406210624797	21.818528406028566	23.08592560012546	27.106139783221185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	2.5
2	5.0
3	5.0
4	5.0
5	13.0
6	21.0
7	258.0
8	495.0
9	994.0
10	1493.0
11	1814.0
12	2135.0
13	1943.5
14	2095.5
15	2439.0
16	2576.0
17	2713.0
18	2736.0
19	2759.0
20	3463.5
21	4168.0
22	4624.5
23	5081.0
24	6600.5
25	8120.0
26	7589.0
27	7058.0
28	9750.0
29	12442.0
30	14187.5
31	15933.0
32	17521.5
33	19110.0
34	22684.5
35	26259.0
36	29789.5
37	33320.0
38	35986.0
39	42374.5
40	46097.0
41	52519.5
42	58942.0
43	64233.5
44	69525.0
45	76776.0
46	84027.0
47	90643.5
48	97260.0
49	104651.0
50	112042.0
51	126983.5
52	141925.0
53	169723.5
54	197522.0
55	208863.5
56	220205.0
57	211603.0
58	203001.0
59	212991.0
60	222981.0
61	193748.0
62	164515.0
63	149148.5
64	107696.5
65	81611.0
66	73750.5
67	65890.0
68	57412.0
69	48934.0
70	49782.0
71	50630.0
72	37500.5
73	24371.0
74	15326.0
75	6281.0
76	4657.5
77	3034.0
78	2614.5
79	2195.0
80	1200.0
81	205.0
82	126.0
83	47.0
84	34.0
85	21.0
86	21.0
87	21.0
88	16.0
89	6.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2219036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.891067479028662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.26426891776343	6.069422213543935
2	10.028151058857276	1.783219355083855
3	3.850713169866186	1.0271085190699385
4	1.8217593034204358	0.6478953958903738
5	0.9879608609008874	0.4392013340454519
6	0.6240251594711828	0.3328949880881944
7	0.4672857337938214	0.2908268293803803
8	0.3610033366953618	0.25677640213703723
9	0.30922805462765873	0.24744307500929474
>10	7.826361845773501	19.845565740946764
>50	3.593104006449939	22.14629754270202
>100	1.7254416609787615	29.182075983853473
>500	0.09091965184433272	5.470470719310085
>1k	0.04469792939833117	8.074108412941802
>5k	0.003047586095340762	1.85713480435046
>10k+	0.002031724063560508	2.3295586836469653
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGTTTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	16906	0.7618623582492579	No Hit
AGTTTAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	12019	0.5416315913757145	No Hit
AGTTTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	11520	0.5191443491678369	No Hit
AGTTTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	11139	0.5019747313698381	No Hit
AGTTTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8664	0.3904398126033106	No Hit
AGTTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7553	0.34037302684589166	No Hit
AGTTTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	6995	0.31522697243307457	No Hit
AGTTTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	6576	0.2963448993166402	No Hit
AGTTTAGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6319	0.28476329361037855	No Hit
AGTTTAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	5016	0.22604410203349562	No Hit
AGTTTAGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4932	0.22225867448748016	No Hit
AGTTTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	4602	0.2073873519852765	No Hit
AGTTTAGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	4529	0.20409763518933446	No Hit
AGTTTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	4365	0.19670703855187566	No Hit
AGTTTAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	4277	0.19274135255128805	No Hit
AGTTTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	4246	0.19134434952835377	No Hit
AGTTTAGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4084	0.18404388211818104	No Hit
AGTTTAGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3972	0.1789966453901604	No Hit
AGTTTAGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	3971	0.17895158077651738	No Hit
AGTTTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3801	0.17129059645720032	No Hit
AGTTTAGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	3748	0.16890217193411913	No Hit
AGTTTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	3535	0.15930340922815134	No Hit
AGTTTAGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	3494	0.15745576006878662	No Hit
AGTTTAGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	3357	0.15128190799968996	No Hit
AGTTTAGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3334	0.1502454218859	No Hit
AGTTTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	3271	0.1474063512263884	No Hit
AGTTTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	3240	0.14600934820345413	No Hit
AGTTTAGGGGACCTTGCCTGCGCCCGCGCGAGCTTAGCAGATCTCCACTTAC	3045	0.13722174854306105	No Hit
AGTTTAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3035	0.13677110240663062	No Hit
AGTTTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2820	0.12708221047337673	No Hit
AGTTTAGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA	2779	0.12523456131401203	No Hit
AGTTTAGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	2586	0.11653709088090504	No Hit
AGTTTAGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2565	0.11559073399440119	No Hit
AGTTTAGGGGCTTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGA	2555	0.11514008785797075	No Hit
AGTTTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	2510	0.1131121802440339	No Hit
AGTTTAGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	2509	0.11306711563039086	No Hit
AGTTTAGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	2480	0.11176024183474265	No Hit
AGTTTAGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2447	0.11027310958452229	No Hit
AGTTTAGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC	2236	0.10076447610584055	No Hit
AGTTTAGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	2228	0.10040395919669622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.014150288683914998	0.0	0.0	0.0	0.0
7	0.014330547138487163	0.0	0.0	0.0	0.0
8	0.014736128661274536	0.0	0.0	0.0	0.0
9	0.015682485547778405	0.0	0.0	0.0	0.0
10	0.027669672776827414	0.0	0.0	0.0	0.0
11	0.05033717343927724	0.0	0.0	0.0	0.0
12	0.06209903760011104	0.0	0.0	0.0	0.0
13	0.07106689571507628	0.0	0.0	0.0	0.0
14	0.08228798451219359	0.0	0.0	0.0	0.0
15	0.0922021995136627	0.0	0.0	0.0	0.0
16	0.10184602683327355	0.0	0.0	0.0	0.0
17	0.11054349726638053	0.0	0.0	0.0	0.0
18	0.11797915851748236	0.0	0.0	0.0	0.0
19	0.12838908426902493	0.0	0.0	0.0	0.0
20	0.13659084395205845	0.0	0.0	0.0	0.0
21	0.14330547138487162	0.0	0.0	0.0	0.0
22	0.1505608741814013	0.0	0.0	0.0	0.0
23	0.15898795693265003	0.0	0.0	0.0	0.0
24	0.16827126734311657	0.0	0.0	0.0	0.0
25	0.17633783318522098	0.0	0.0	0.0	0.0
26	0.1844043990273254	0.0	0.0	0.0	0.0
27	0.19274135255128805	0.0	0.0	0.0	0.0
28	0.20878435500821077	0.0	0.0	0.0	0.0
29	0.21770714850953296	0.0	0.0	0.0	0.0
30	0.22901836653393637	0.0	0.0	0.0	0.0
31	0.2369046739214686	0.0	0.0	0.0	0.0
32	0.24740472890029724	0.0	0.0	0.0	0.0
33	0.2556515531969738	0.0	0.0	0.0	0.0
34	0.26470954053922513	0.0	0.0	0.0	0.0
35	0.2796259276550718	0.0	0.0	0.0	0.0
36	0.29071182261126005	0.0	0.0	0.0	0.0
37	0.2992740992034379	0.0	0.0	0.0	0.0
38	0.30774624656832966	0.0	0.0	0.0	0.0
39	0.3165789108423658	0.0	0.0	0.0	0.0
40	0.3251862520481867	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGT	25	3.4181914E-5	46.0	24
CACTACG	20	6.3127314E-4	46.0	46
CGTGTAT	25	3.4181914E-5	46.0	15
ACCGTTT	45	3.110472E-10	46.0	19
ACGTATA	20	6.3127314E-4	46.0	40
CGACTAA	20	6.3127314E-4	46.0	23
AATAACG	45	3.110472E-10	46.0	46
TAGGGGC	27910	0.0	44.912216	5
TAGGGGA	42650	0.0	44.813602	5
AGTTTAG	227395	0.0	44.76299	1
TAGGGAT	17250	0.0	44.76	5
TTAGGGG	141820	0.0	44.726906	4
GTTTAGG	225640	0.0	44.710556	2
TTTAGGG	222245	0.0	44.71052	3
TAGGGGT	21460	0.0	44.456665	5
TAGGGGG	50095	0.0	44.402233	5
AGGGATT	5845	0.0	44.189903	6
TTAGGGA	61120	0.0	44.12598	4
TAGGGAG	22835	0.0	44.045982	5
CGACAAA	115	0.0	44.0	43
>>END_MODULE