FastQCFastQC Report
Fri 17 Jun 2016
SRR1527552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences360424
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT22740.630923578895967No Hit
ATTTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC10590.29382061128004794No Hit
ATTTGCGGGGGCGGAGTCCGGCTCTCGCAACCCAAGATGGCGGATGAGAG8250.2288970767762413No Hit
ATTTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC8130.22556766475040507No Hit
ATTTGCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTG6820.1892215834683595No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT6650.18450491643175815No Hit
ATTTGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA6570.182285308414534No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGCGCTTAAGAGCGCAGACTGC6460.17923334739085078No Hit
ATTTGCGGGATCCTCACCCTGCCACCCTAACCCACTAAGTGCCTGTGCGC6280.17423922935209643No Hit
ATTTGCGGGGGACTCTGAGGCTCTTCCGCTGAGTTTAACTAGCTGTGGCT6200.17201962133487225No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT6150.17063236632410717No Hit
ATTTGCGGGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGAA6130.17007746431980114No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT6010.1667480522939649No Hit
ATTTGCGGGATGGTGACACCCATTTGTAATCCCAGCAAGTGGGAGGTAGA5960.16536079728319977No Hit
ATTTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG5910.16397354227243469No Hit
ATTTGCGGGGGTCCTCCCGCGCCTCCTCCACGACCTCCAGTGAGCCTCCG5720.15870197323152732No Hit
ATTTGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCT5170.14344216811311122No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC5120.14205491310234614No Hit
ATTTGCGGGAGCGTCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACG5060.140390207089428No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG4940.13706079506359176No Hit
ATTTGCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA4900.13595099105497968No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACT4860.1348411870463676No Hit
ATTTGCGGGGGTATCAATGAGAGTGCAGTGCTATTTGCGGGTGCAAAGCT4780.13262157902914345No Hit
ATTTGCGGGTCTCGCAACCCAAGATGGCGGATGAGAGTGAGACAGCAGTG4780.13262157902914345No Hit
ATTTGCGGGATCTGACACTGCCTTCCTTCTTGGCCCTGTGGTACTACTGT4760.1320666770248374No Hit
ATTTGCGGGTGCTGTGCTGCCCATGGTGGCGCATGCCTTTAATCCCAGCA4600.1276274609903891No Hit
ATTTGCGGACTCGCCCTGTATTGACCAAGGAGCAGCTGGACAACCAATTG4580.12707255898608305No Hit
ATTTGCGGGAGTGAAAATATTTTTAAGGTCTAATCAAGTGATTTTTTCCC4580.12707255898608305No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG4540.12596275497747098No Hit
ATTTGCGGGATGTGAACCCACTTCCGACCGGCTATGAAGATGAGTAAAGA4500.1248529509688589No Hit
ATTTGCGGGGATCTCAGCCATGGGCAAAAAGCACAAGAAGCACAAGGCGG4500.1248529509688589No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC4280.11874902892149246No Hit
ATTTGCGGCACTTCAAAGCAGCGAGGATTGCTATTGAGATCTATTTGAAG4280.11874902892149246No Hit
ATTTGCGGGACCAAAGTGATCCTTCACCTCAAAGAAGACCAGACAGAGTA4270.11847157791933945No Hit
ATTTGCGGGAGACGCGGGCCCGCCATGGAGCACATCCGCACGCCCAAGGT4260.11819412691718642No Hit
ATTTGCGGGGAAACGCTGGCTAAAGCGGTGTTGGCGGAGCTGCCTCAGCA4210.11680687190642133No Hit
ATTTGCGGGGCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGT4180.11597451889996226No Hit
ATTTGCGGGGGAGTACCAGCTCCCTATTACCCTGAGGGCGGGCGAGTGCA4050.112367655871973No Hit
ATTTGCGGGAGATTATTGAAGTTTCCACAGACGGTCAGCTGTTGAGAGGT4040.11209020486981999No Hit
ATTTGCGGGGGCCCCAGCCGCCGCCAGCACCATGGCCAGCACCGTATCTC4030.11181275386766697No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC4000.11098040086120792No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGC3990.11070294985905489No Hit
ATTTGCGGGAAGGCTGAGCTGGAGAAGCTGGACCTCAATGGCAATGCCCT3850.1068186358289126No Hit
ATTTGCGGAGAGTGCAGTGCTATTTGGGGGGATGCAAGAGCCTAGTGCGG3840.1065411848267596No Hit
ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG3840.1065411848267596No Hit
ATTTGCGGGGAGTAGCCGGACCCCGCCTGCGCCCCAGGGGGTGCCTCCTC3830.10626373382460656No Hit
ATTTGCAGGGGCCTGGTGCTGGAACCAGGAAGGCGCTGAGCTTAAATTGA3770.10459902781168845No Hit
ATTTGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG3760.10432157680953544No Hit
ATTTGCGGGAGCATCAAATTAAAACATTCCAAGAAGAAGAGGAAGAAGAA3750.10404412580738243No Hit
ATTTGCGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAA3730.10348922380307639No Hit
ATTTGCGGGGATTGTTACCCAACAGTAAGGGCCTTTTCTTCCCTGTCAGT3690.1023794197944643No Hit
ATTTGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC3690.1023794197944643No Hit
ATTTGCAGGGGAGCTAGTGCGTCCGCAGTGATGAGCTACGACCGCGCCAT3620.10043726277939316No Hit
ATTTGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC3620.10043726277939316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAC207.8492804E-444.00000410
AACCGCA207.8492804E-444.00000431
AAATCGA207.8492804E-444.00000415
TACCATA207.8492804E-444.00000412
AATCAAC207.8492804E-444.00000412
TACCAAT207.8492804E-444.00000438
GCAACCG207.8492804E-444.00000429
GGCGTTA408.285497E-944.00000443
CGCATGA207.8492804E-444.00000422
TTACTCG207.8492804E-444.00000438
AGGGTAA207.8492804E-444.00000415
ATCAATC207.8492804E-444.00000425
CGTTACC207.8492804E-444.00000439
CGTTAAA408.285497E-944.00000431
TCAATTA207.8492804E-444.00000441
GTTACAC207.8492804E-444.00000436
TCGGATA207.8492804E-444.00000436
CCACTCG207.8492804E-444.00000429
CACTCGG207.8492804E-444.00000430
TCGGAAT207.8492804E-444.00000435
Produced by FastQC (version 0.11.5)