##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527552_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	360424
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.740333607084988	31.0	30.0	33.0	25.0	33.0
2	30.60521774354649	31.0	31.0	33.0	28.0	34.0
3	30.96382593833929	31.0	31.0	33.0	28.0	34.0
4	35.57290579983575	37.0	35.0	37.0	33.0	37.0
5	35.56340587752203	37.0	35.0	37.0	33.0	37.0
6	36.12777451002153	37.0	36.0	37.0	35.0	37.0
7	36.373229862606266	37.0	37.0	37.0	35.0	37.0
8	36.5742014960158	37.0	37.0	37.0	35.0	37.0
9	38.55439981799214	39.0	39.0	39.0	38.0	39.0
10	36.75324340221517	39.0	37.0	39.0	32.0	39.0
11	36.8135945442035	38.0	37.0	39.0	33.0	39.0
12	36.60802554768828	38.0	35.0	39.0	33.0	39.0
13	36.80952433800191	39.0	37.0	39.0	33.0	39.0
14	37.72265720373782	39.0	37.0	40.0	33.0	40.0
15	37.81169955385879	40.0	38.0	40.0	33.0	40.0
16	37.91789115042283	40.0	38.0	40.0	34.0	40.0
17	37.81431314229907	40.0	37.0	40.0	34.0	40.0
18	37.64511797216611	39.0	37.0	40.0	33.0	40.0
19	37.522343129203385	39.0	37.0	40.0	33.0	40.0
20	37.358730273233746	39.0	36.0	40.0	33.0	40.0
21	37.22133931147759	38.0	36.0	40.0	33.0	40.0
22	36.954683927818344	38.0	36.0	40.0	33.0	40.0
23	36.631284265198765	38.0	35.0	40.0	32.0	40.0
24	36.50428661798326	38.0	35.0	40.0	32.0	40.0
25	36.221089605575656	38.0	35.0	39.0	31.0	40.0
26	35.72054857612146	37.0	34.0	39.0	31.0	40.0
27	35.49228686214015	37.0	34.0	39.0	30.0	40.0
28	35.26448016780237	37.0	34.0	38.0	30.0	40.0
29	34.93123931813642	36.0	33.0	38.0	30.0	40.0
30	34.643755687745546	36.0	33.0	38.0	29.0	39.0
31	34.44059774043904	36.0	33.0	38.0	29.0	39.0
32	34.375285774532216	36.0	33.0	38.0	29.0	39.0
33	33.81765642687501	35.0	32.0	38.0	27.0	39.0
34	33.32268383903403	34.0	31.0	38.0	26.0	39.0
35	33.08961112467538	34.0	31.0	37.0	26.0	38.0
36	32.76884169755621	34.0	31.0	37.0	26.0	38.0
37	32.57907353561361	34.0	31.0	36.0	26.0	38.0
38	32.09652520364904	34.0	30.0	36.0	24.0	38.0
39	31.33191463387566	33.0	29.0	36.0	23.0	38.0
40	31.037070228397663	33.0	29.0	35.0	23.0	38.0
41	30.66516103256165	32.0	29.0	35.0	22.0	37.0
42	30.419270081903537	32.0	28.0	34.0	22.0	37.0
43	29.945744456528978	31.0	27.0	34.0	21.0	37.0
44	29.59343162497503	31.0	27.0	34.0	21.0	36.0
45	29.148106119459303	31.0	26.0	34.0	20.0	36.0
46	28.937179544092512	31.0	26.0	34.0	20.0	36.0
47	28.34796794886023	30.0	26.0	33.0	20.0	35.0
48	27.813602867733557	29.0	24.0	33.0	18.0	35.0
49	27.119548087807694	29.0	24.0	32.0	18.0	34.0
50	30.832633231971233	32.0	29.0	34.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	6.0
14	27.0
15	51.0
16	62.0
17	95.0
18	172.0
19	229.0
20	388.0
21	594.0
22	934.0
23	1292.0
24	1877.0
25	2559.0
26	3521.0
27	4553.0
28	5863.0
29	8068.0
30	11077.0
31	16712.0
32	28077.0
33	43197.0
34	61472.0
35	89117.0
36	71329.0
37	8944.0
38	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	98.54421459170311	1.320666770248374	0.04661176836170732	0.08850686968681332
8	99.45064701573702	0.3812176769582492	0.1348411870463676	0.03329412025836237
9	97.69216256409118	1.63723836370497	0.24831864692695269	0.42228042527689613
10	49.40986171842053	37.15651565933456	4.791301356180498	8.642321266064414
11	34.21553503651255	24.713115663773777	24.27224602135263	16.79910327836104
12	27.069784476061525	19.548365258695313	27.929327680731582	25.452522584511577
13	23.01650278560806	19.10055934122034	31.249028921492467	26.633908951679135
14	22.9926419994229	21.85953210662997	28.888753246176723	26.259072647770402
15	22.85641355736577	23.13774887354893	26.50378443166937	27.50205313741593
16	27.24430115641578	23.814174416797993	24.882360775087122	24.05916365169911
17	28.990577763966886	23.36192928328857	22.92633120990833	24.721161742836216
18	27.7195747231039	22.213837036379374	24.44537544669611	25.621212793820614
19	28.237298293121434	22.155849776929397	22.689110603067498	26.91774132688167
20	29.552416043326744	22.252125274676494	22.409439992897255	25.786018689099503
21	30.049330788182804	20.6906310345593	24.814662730561782	24.44537544669611
22	29.23667680287661	19.87769959825095	25.744678489778707	25.14094510909373
23	23.890750893392227	23.892415599405144	27.397176658602092	24.819656848600538
24	26.016580471888663	23.69709009388942	26.326770692295742	23.959558741926177
25	26.755987392626462	25.068807848533947	23.24789692140368	24.927307837435908
26	26.057088318203004	25.799891239207156	21.948871329323243	26.194149113266597
27	25.774643198011233	22.87639002952079	25.923634386167404	25.425332386300582
28	29.12153463698311	21.522429139014047	23.283133198677113	26.07290302532573
29	25.994661842718575	23.808070494750627	24.414300934454975	25.782966728075824
30	28.08969435997603	20.842396732736997	23.49038909728542	27.577519810001554
31	28.863782655982956	23.0658890639913	22.00019976472155	26.070128515304198
32	29.228908174816326	23.917941091603222	22.66802432690387	24.18512640667658
33	29.82542782944532	21.818469358311322	23.438228308880653	24.917874503362704
34	26.74821876456618	25.013595099105494	26.12811577475418	22.110070361574145
35	28.23174927307838	23.98036756708765	25.717765742569863	22.07011741726411
36	26.55677757308059	24.73531174394602	23.843861674028368	24.86404900894502
37	27.380807049475063	26.413890306971787	22.28014782589395	23.9251548176592
38	29.15455130623932	25.39148336403791	22.692994917097643	22.76097041262513
39	28.988358155949662	23.97731560606397	23.032040041728628	24.002286196257742
40	28.564967926664153	24.97059019377178	23.50592635340599	22.95851552615808
41	25.014150001109805	24.35131955696624	25.19976472155017	25.434765720373782
42	25.61150200874526	23.711240094999223	24.304152886600228	26.373105009655294
43	24.997502940980624	23.005959647526247	26.024904001953253	25.971633409539873
44	25.47860297871396	23.159390051716866	25.03773333629281	26.324273633276363
45	24.711728408763012	22.7717910017091	25.221683350720266	27.29479723880763
46	28.005904157325816	23.475406743169156	24.67454997447451	23.84413912503052
47	26.16973342507713	25.08434510465452	25.09100392870619	23.65491754156216
48	27.090315850220854	23.64215479546312	25.01109804008612	24.256431314229907
49	26.3045746121235	24.578551927729563	25.062149024482277	24.054724435664664
50	23.953732270880963	23.66684793465474	26.931058974985017	25.448360819479284
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	3.0
4	6.0
5	3.0
6	0.0
7	8.0
8	16.0
9	79.0
10	142.0
11	216.0
12	290.0
13	304.5
14	319.0
15	420.0
16	521.0
17	464.5
18	408.0
19	536.5
20	665.0
21	541.0
22	417.0
23	1138.0
24	1859.0
25	2021.0
26	2183.0
27	2374.5
28	2566.0
29	3188.5
30	3811.0
31	4721.0
32	5631.0
33	6367.0
34	7103.0
35	8762.0
36	10421.0
37	10876.5
38	11332.0
39	12189.0
40	13046.0
41	13093.5
42	13141.0
43	13599.5
44	14058.0
45	16167.5
46	18277.0
47	19028.5
48	19780.0
49	19349.5
50	18919.0
51	20009.5
52	21100.0
53	23249.0
54	25398.0
55	26364.0
56	27330.0
57	27147.0
58	26964.0
59	27704.5
60	28445.0
61	26029.0
62	23613.0
63	22181.5
64	20750.0
65	15959.0
66	11168.0
67	10478.0
68	9788.0
69	8752.5
70	7717.0
71	5765.5
72	3814.0
73	4913.0
74	6012.0
75	4269.0
76	2526.0
77	1527.5
78	529.0
79	376.0
80	223.0
81	178.5
82	134.0
83	68.0
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	360424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.576365613832596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.20122711149457	8.242514371961912
2	9.407055891093854	2.1779903669012053
3	3.1708369283865405	1.1012030275453355
4	1.3996740485092511	0.6481255410294542
5	0.7070271306682006	0.40924022817570416
6	0.45058000191736175	0.3129647304286063
7	0.34752180998945453	0.2816127671853151
8	0.29239766081871343	0.2707921781013473
9	0.30198446937014667	0.3146294364415244
>10	8.3525069504362	26.857534459414467
>50	2.8928194803949765	23.035369453754466
>100	1.4308311763014092	32.465929016935604
>500	0.04074393634359122	2.9573502319490377
>1k	0.004793404275716614	0.924744190176015
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2274	0.630923578895967	No Hit
ATTTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1059	0.29382061128004794	No Hit
ATTTGCGGGGGCGGAGTCCGGCTCTCGCAACCCAAGATGGCGGATGAGAG	825	0.2288970767762413	No Hit
ATTTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	813	0.22556766475040507	No Hit
ATTTGCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTG	682	0.1892215834683595	No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	665	0.18450491643175815	No Hit
ATTTGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	657	0.182285308414534	No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGCGCTTAAGAGCGCAGACTGC	646	0.17923334739085078	No Hit
ATTTGCGGGATCCTCACCCTGCCACCCTAACCCACTAAGTGCCTGTGCGC	628	0.17423922935209643	No Hit
ATTTGCGGGGGACTCTGAGGCTCTTCCGCTGAGTTTAACTAGCTGTGGCT	620	0.17201962133487225	No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT	615	0.17063236632410717	No Hit
ATTTGCGGGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGAA	613	0.17007746431980114	No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	601	0.1667480522939649	No Hit
ATTTGCGGGATGGTGACACCCATTTGTAATCCCAGCAAGTGGGAGGTAGA	596	0.16536079728319977	No Hit
ATTTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	591	0.16397354227243469	No Hit
ATTTGCGGGGGTCCTCCCGCGCCTCCTCCACGACCTCCAGTGAGCCTCCG	572	0.15870197323152732	No Hit
ATTTGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCT	517	0.14344216811311122	No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	512	0.14205491310234614	No Hit
ATTTGCGGGAGCGTCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACG	506	0.140390207089428	No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	494	0.13706079506359176	No Hit
ATTTGCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA	490	0.13595099105497968	No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACT	486	0.1348411870463676	No Hit
ATTTGCGGGGGTATCAATGAGAGTGCAGTGCTATTTGCGGGTGCAAAGCT	478	0.13262157902914345	No Hit
ATTTGCGGGTCTCGCAACCCAAGATGGCGGATGAGAGTGAGACAGCAGTG	478	0.13262157902914345	No Hit
ATTTGCGGGATCTGACACTGCCTTCCTTCTTGGCCCTGTGGTACTACTGT	476	0.1320666770248374	No Hit
ATTTGCGGGTGCTGTGCTGCCCATGGTGGCGCATGCCTTTAATCCCAGCA	460	0.1276274609903891	No Hit
ATTTGCGGACTCGCCCTGTATTGACCAAGGAGCAGCTGGACAACCAATTG	458	0.12707255898608305	No Hit
ATTTGCGGGAGTGAAAATATTTTTAAGGTCTAATCAAGTGATTTTTTCCC	458	0.12707255898608305	No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	454	0.12596275497747098	No Hit
ATTTGCGGGATGTGAACCCACTTCCGACCGGCTATGAAGATGAGTAAAGA	450	0.1248529509688589	No Hit
ATTTGCGGGGATCTCAGCCATGGGCAAAAAGCACAAGAAGCACAAGGCGG	450	0.1248529509688589	No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	428	0.11874902892149246	No Hit
ATTTGCGGCACTTCAAAGCAGCGAGGATTGCTATTGAGATCTATTTGAAG	428	0.11874902892149246	No Hit
ATTTGCGGGACCAAAGTGATCCTTCACCTCAAAGAAGACCAGACAGAGTA	427	0.11847157791933945	No Hit
ATTTGCGGGAGACGCGGGCCCGCCATGGAGCACATCCGCACGCCCAAGGT	426	0.11819412691718642	No Hit
ATTTGCGGGGAAACGCTGGCTAAAGCGGTGTTGGCGGAGCTGCCTCAGCA	421	0.11680687190642133	No Hit
ATTTGCGGGGCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGT	418	0.11597451889996226	No Hit
ATTTGCGGGGGAGTACCAGCTCCCTATTACCCTGAGGGCGGGCGAGTGCA	405	0.112367655871973	No Hit
ATTTGCGGGAGATTATTGAAGTTTCCACAGACGGTCAGCTGTTGAGAGGT	404	0.11209020486981999	No Hit
ATTTGCGGGGGCCCCAGCCGCCGCCAGCACCATGGCCAGCACCGTATCTC	403	0.11181275386766697	No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	400	0.11098040086120792	No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGC	399	0.11070294985905489	No Hit
ATTTGCGGGAAGGCTGAGCTGGAGAAGCTGGACCTCAATGGCAATGCCCT	385	0.1068186358289126	No Hit
ATTTGCGGAGAGTGCAGTGCTATTTGGGGGGATGCAAGAGCCTAGTGCGG	384	0.1065411848267596	No Hit
ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	384	0.1065411848267596	No Hit
ATTTGCGGGGAGTAGCCGGACCCCGCCTGCGCCCCAGGGGGTGCCTCCTC	383	0.10626373382460656	No Hit
ATTTGCAGGGGCCTGGTGCTGGAACCAGGAAGGCGCTGAGCTTAAATTGA	377	0.10459902781168845	No Hit
ATTTGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG	376	0.10432157680953544	No Hit
ATTTGCGGGAGCATCAAATTAAAACATTCCAAGAAGAAGAGGAAGAAGAA	375	0.10404412580738243	No Hit
ATTTGCGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAA	373	0.10348922380307639	No Hit
ATTTGCGGGGATTGTTACCCAACAGTAAGGGCCTTTTCTTCCCTGTCAGT	369	0.1023794197944643	No Hit
ATTTGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	369	0.1023794197944643	No Hit
ATTTGCAGGGGAGCTAGTGCGTCCGCAGTGATGAGCTACGACCGCGCCAT	362	0.10043726277939316	No Hit
ATTTGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	362	0.10043726277939316	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	5.549020043060396E-4	0.0	0.0	0.0	0.0
9	0.0013872550107650989	0.0	0.0	0.0	0.0
10	0.007491177058131534	0.0	0.0	0.0	0.0
11	0.013317648103344949	0.0	0.0	0.0	0.0
12	0.021918629170088563	0.0	0.0	0.0	0.0
13	0.028022551217454998	0.0	0.0	0.0	0.0
14	0.03301666925620935	0.0	0.0	0.0	0.0
15	0.042727454331565044	0.0	0.0	0.0	0.0
16	0.05105098439615564	0.0	0.0	0.0	0.0
17	0.05881961245644019	0.0	0.0	0.0	0.0
18	0.06464608350165361	0.0	0.0	0.0	0.0
19	0.070195103544714	0.0	0.0	0.0	0.0
20	0.07546667258562138	0.0	0.0	0.0	0.0
21	0.07907353561361063	0.0	0.0	0.0	0.0
22	0.08295784964375291	0.0	0.0	0.0	0.0
23	0.08822941868466029	0.0	0.0	0.0	0.0
24	0.0940558897298737	0.0	0.0	0.0	0.0
25	0.09988236077508712	0.0	0.0	0.0	0.0
26	0.10792843983752469	0.0	0.0	0.0	0.0
27	0.112367655871973	0.0	0.0	0.0	0.0
28	0.11874902892149246	0.0	0.0	0.0	0.0
29	0.1273500099882361	0.0	0.0	0.0	0.0
30	0.13567354005282667	0.0	0.0	0.0	0.0
31	0.1417774621001931	0.0	0.0	0.0	0.0
32	0.15093334517124277	0.0	0.0	0.0	0.0
33	0.15675981621645618	0.0	0.0	0.0	0.0
34	0.16647060129181188	0.0	0.0	0.0	0.0
35	0.17285197434133132	0.0	0.0	0.0	0.0
36	0.18034315139946286	0.0	0.0	0.0	0.0
37	0.18727942645328835	0.0	0.0	0.0	0.0
38	0.19671276052649103	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAAC	20	7.8492804E-4	44.000004	10
AACCGCA	20	7.8492804E-4	44.000004	31
AAATCGA	20	7.8492804E-4	44.000004	15
TACCATA	20	7.8492804E-4	44.000004	12
AATCAAC	20	7.8492804E-4	44.000004	12
TACCAAT	20	7.8492804E-4	44.000004	38
GCAACCG	20	7.8492804E-4	44.000004	29
GGCGTTA	40	8.285497E-9	44.000004	43
CGCATGA	20	7.8492804E-4	44.000004	22
TTACTCG	20	7.8492804E-4	44.000004	38
AGGGTAA	20	7.8492804E-4	44.000004	15
ATCAATC	20	7.8492804E-4	44.000004	25
CGTTACC	20	7.8492804E-4	44.000004	39
CGTTAAA	40	8.285497E-9	44.000004	31
TCAATTA	20	7.8492804E-4	44.000004	41
GTTACAC	20	7.8492804E-4	44.000004	36
TCGGATA	20	7.8492804E-4	44.000004	36
CCACTCG	20	7.8492804E-4	44.000004	29
CACTCGG	20	7.8492804E-4	44.000004	30
TCGGAAT	20	7.8492804E-4	44.000004	35
>>END_MODULE