FastQCFastQC Report
Fri 17 Jun 2016
SRR1527551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310188
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG21710.6998981262976002No Hit
ATTTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC9030.29111377616155365No Hit
ATTTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC7180.23147252633886548No Hit
ATTTGCGGGGGCGGAGTCCGGCTCTCGCAACCCAAGATGGCGGATGAGAGTG7100.2288934452654519No Hit
ATTTGCGGGGGTCCTCCCGCGCCTCCTCCACGACCTCCAGTGAGCCTCCGCG5580.1798909048705946No Hit
ATTTGCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT5510.17763420893135776No Hit
ATTTGCGGGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGAATC5450.1756998981262976No Hit
ATTTGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA5430.17505512785794422No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC5410.17441035758959084No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT5350.17247604678453066No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGCGCTTAAGAGCGCAGACTGCTC5340.17215366165035398No Hit
ATTTGCGGGATCCTCACCCTGCCACCCTAACCCACTAAGTGCCTGTGCGCAT5280.17021935084529383No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5200.1676402697718803No Hit
ATTTGCGGGAGCGTCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCG5090.16409403329593664No Hit
ATTTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT4840.15603440494151932No Hit
ATTTGCGGGGGACTCTGAGGCTCTTCCGCTGAGTTTAACTAGCTGTGGCTGT4640.14958670225798548No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT4590.14797477658710204No Hit
ATTTGCGGGTCTCGCAACCCAAGATGGCGGATGAGAGTGAGACAGCAGTGAA4560.14700762118457195No Hit
ATTTGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAA4550.14668523605039524No Hit
ATTTGCGGGAGACGCGGGCCCGCCATGGAGCACATCCGCACGCCCAAGGTTG4280.13798083742762454No Hit
ATTTGCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT4270.13765845229344784No Hit
ATTTGCGGGATCTGACACTGCCTTCCTTCTTGGCCCTGTGGTACTACTGTCT4220.1360465266225644No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG4060.1308883644757373No Hit
ATTTGCGGGATGGTGACACCCATTTGTAATCCCAGCAAGTGGGAGGTAGAGT4010.12927643880485382No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACTCT4010.12927643880485382No Hit
ATTTGCGGGATGTGAACCCACTTCCGACCGGCTATGAAGATGAGTAAAGAGA3990.12863166853650043No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT3960.12766451313397037No Hit
ATTTGCGGAGAGTGCAGTGCTATTTGGGGGGATGCAAGAGCCTAGTGCGGTG3880.1250854320605568No Hit
ATTTGCGGACTCGCCCTGTATTGACCAAGGAGCAGCTGGACAACCAATTGGA3840.12379589152385007No Hit
ATTTGCGGGGGTATCAATGAGAGTGCAGTGCTATTTGCGGGTGCAAAGCTGC3830.12347350638967336No Hit
ATTTGCGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAATG3820.12315112125549667No Hit
ATTTGCGGGGATTGTTACCCAACAGTAAGGGCCTTTTCTTCCCTGTCAGTCA3720.11992726991372975No Hit
ATTTGCGGGGATCTCAGCCATGGGCAAAAAGCACAAGAAGCACAAGGCGGAA3720.11992726991372975No Hit
ATTTGCGGGAGATTATTGAAGTTTCCACAGACGGTCAGCTGTTGAGAGGTTC3690.11896011451119966No Hit
ATTTGCGGGTGCTGTGCTGCCCATGGTGGCGCATGCCTTTAATCCCAGCACT3670.11831534424284627No Hit
ATTTGCGGGGAAACGCTGGCTAAAGCGGTGTTGGCGGAGCTGCCTCAGCAAG3630.11702580370613949No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC3610.11638103343778612No Hit
ATTTGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT3570.11509149290107935No Hit
ATTTGCGGGGGAGACTCGCCATATTGTGCGGCGGCGCCGGCGGTTGATTCTC3560.11476910776690265No Hit
ATTTGCAGGGGCCTGGTGCTGGAACCAGGAAGGCGCTGAGCTTAAATTGAAG3560.11476910776690265No Hit
ATTTGCGGGGGCCCCAGCCGCCGCCAGCACCATGGCCAGCACCGTATCTCCC3520.11347956723019588No Hit
ATTTGCGGGGCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGA3500.11283479696184248No Hit
ATTTGCGGGGAGTAGCCGGACCCCGCCTGCGCCCCAGGGGGTGCCTCCTCTC3490.11251241182766582No Hit
ATTTGCGGCACTTCAAAGCAGCGAGGATTGCTATTGAGATCTATTTGAAGCT3480.11219002669348913No Hit
ATTTGCGGGAGTGAAAATATTTTTAAGGTCTAATCAAGTGATTTTTTCCCCC3470.11186764155931242No Hit
ATTTGCGGGACCAAAGTGATCCTTCACCTCAAAGAAGACCAGACAGAGTACT3450.11122287129095904No Hit
ATTTGCGGGGGAGTACCAGCTCCCTATTACCCTGAGGGCGGGCGAGTGCAGC3450.11122287129095904No Hit
ATTTGCAGGGGAGCTAGTGCGTCCGCAGTGATGAGCTACGACCGCGCCATCA3380.10896617535172218No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTC3320.10703186454666203No Hit
ATTTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA3310.10670947941248533No Hit
ATTTGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG3260.10509755374160186No Hit
ATTTGCGGAACACAAGTGTAACATCGATGGAAGAAGACACCAACCCAGAGGT3260.10509755374160186No Hit
ATTTGCGGGGGACTGGGGCTCGCCGTCGCCAGCAGTCAGCACTGCGACCTCG3250.10477516860742517No Hit
ATTTGCGGGGCCAGACCCGCCCCGACTGGGGCTCGCCGTCGCCAGCAGTCAG3230.1041303983390718No Hit
ATTTGCGGGGGCTTGGGACGCCGCGGATCTGCGGCGGCGACAGTTGGAGAGG3220.1038080132048951No Hit
ATTTGCGGGGGCCAGTACTTATTTTTTTTAAAGAATTATTATTTTTTTTAAT3190.10284085780236502No Hit
ATTTGCGGGAGCTATGTCCGCAAGCCGACCAGGGAAACCCTAGAGCTTTGAG3190.10284085780236502No Hit
ATTTGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA3180.10251847266818832No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3120.10058416186312817No Hit
ATTTGCGGGACCAGTTTTGGCTAAGTAGTGGGTTCTAGATCAGCCTAAACTT3120.10058416186312817No Hit
ATTTGCGGGGAGTTGGAGAGGGACGTTCCCGGGAAGTTACCGGCCAGAGAAA3110.10026177672895148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCAT551.8189894E-1246.00000427
GCGAGAC206.304293E-446.05
CGGACAA206.304293E-446.032
AGCAATA253.411348E-546.010
AACGTAA253.411348E-546.026
CGTGGAA253.411348E-546.020
TCGTTTA206.304293E-446.013
GATACGA206.304293E-446.09
CCCTCGA206.304293E-446.014
ACGCCCA405.5879354E-946.040
CTATCTC206.304293E-446.015
CGACGCT206.304293E-446.025
CGACGAA253.411348E-546.038
ACCATCG253.411348E-546.042
CTATCAA206.304293E-446.027
GACACGA301.856979E-646.014
CCCTAAA206.304293E-446.023
GGCACGT253.411348E-546.015
ACCGCTA206.304293E-446.027
TTAGATT253.411348E-546.038
Produced by FastQC (version 0.11.5)